Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00865431
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4155nts
Open reading frame: 1 to 4155
ATGATGCATCTCCGGCTCTTCTGCATCCTGCTCGCCGCGGTCTCAGGAGCCGAGGGCTGG
GGCTACTACGGCTGCGACGAGGAGCTGGTGGGTCCCCTGTATGCACGCTCCCTGGGCGCC
TCCTCCTACTACAGTCTCCTTACTGCGCCGAGATTCGCCAGGCTGCACGGCATAAGCGGG
TGGTCACCACGGATTGGGGATCCGAATCCCTGGCTCCAGATAGACTTAATGAAGAAGCAC
CGGATCCGGGCCGTGGCCACACAGGGCTCCTTTAATTCTTGGGACTGGGTCACACGTTAC
ATGCTACTCTACGGCGACCGAGTGGACAGCTGGACACCGTTCTACCAGCGAGGGCACAAC
TCGACCTTCTTTGGTAACGTGAACGAGTCGGCGGTGGTGCGCCATGACCTGCACTTCCAC
TTCACTGCGCGCTACATCCGCATCGTGCCCCTGGCCTGGAACCCACGCGGCAAGATCGGC
CTGAGGCTCGGCCTCTATGGCTGCCCATACAAGGCCGACATACTCTATTTCGACGGCGAC
GATGCCATCTCCTACCGCTTCCCGCGAGGGGTCAGCCGAAGCCTGTGGGACGTGTTCGCC
TTCAGCTTCAAGACCGAGGAGAAGGACGGTCTTCTGCTGCACGCCGAGGGCGCCCAGGGC
GACTACGTGACGCTCGAGCTGGAGGGGGCACACCTGCTGCTGCACATGAGCCTGGGCAGC
AGCCCTATCCAGCCAAGACCAGGTCACACCACCGTGAGCGCAGGCGGAGTCCTCAATGAC
CAGCACTGGCACTATGTGCGGGTGGACCGATTTGGCCGCGATGTAAATTTCACCCTGGAC
GGCTATGTGCAGCGCTTTATTCTCAATGGAGACTTCGAGAGGCTGAACCTGGACACTGAG
ATGTTCATCGGAGGTCTGGTGGGCGCCGCGCGGAAGAACCTGGCCTATCGGCATAACTTC
CGCGGCTGCATAGAAAACGTAATCTTCAACCGCGTCAACATCGCAGACCTGGCCGTGCGG
CGCCATTCCCGGATCACCTTCGAGGGTAAGGTGGCTTTTCGTTGCCTGGACCCGGTACCG
CACCCTATCAACTTCGGAGGCCCTCACAACTTCGTTCAAGTGCCCGGTTTCCCACGCCGT
GGCCGCCTGGCAGTCTCATTTCGCTTCCGCACCTGGGACCTCACCGGGCTTCTCCTTTTC
TCCCGTCTGGGGGACGGGCTGGGCCACGTGGAGCTGACGCTCAGCGAAGGGCAGGTCAAC
GTGTCCATCGCGCAGAGCGGCCGAAAGAAGCTTCAGTTCGCTGCTGGGTACCGACTGAAT
GACGGCTTTTGGCACGAGGTGAATTTTGTGGCACAGGAAAACCATGCAGTTATCAGCATT
GATGATGTGGAAGGGGCAGAGGTCAGGGTCTCATACCCGTTGCTGATCCGGACAGGGACC
TCATATTTCTTTGGGGGTTGTCCCAAGCCAGCCAGTCGATGGGACTGCCACTCCAACCAG
ACGGCATTCCATGGCTGCATGGAGCTGCTCAAGGTGGATGGTCAACTGGTCAACCTGACT
CTGGTGGAGGGCCGGCGGCTTGGATTCTATGCTGAGGTCCTCTTTGATACATGTGGCATC
ACTGATAGGTGCAGCCCTAACATGTGTGAGCATGATGGACGCTGCTACCAGTCTTGGGAT
GACTTCATTTGCTACTGCGAACTGACGGGCTACAAGGGAGAGACCTGCCACACACCTTTG
TATAAGGAATCCTGTGAGGCTTATCGGCTCAGTGGGAAAACTTCTGGAAACTTCACCATT
GATCCTGATGGCAGTGGCCCCCTGAAGCCATTTGTAGTGTACTGTGATATCCGAGAGAAC
CGAGCGTGGACAGTTGTGCGGCATGACAGGCTGTGGACAACTCGAGTGACAGGTTCCAGC
ATGGAGCGGCCATTCCTGGGGGCTATCCAGTACTGGAATGCATCCTGGGAGGAAGTCAGT
GCCCTTGCCAATGCTTCCCAGCATTGTGAACAGTGGATCGAGTTCTCCTGCTACAATTCC
CGGCTGCTCAACACTGCAGGAGGCTACCCCTACAGCTTTTGGATTGGCCGAAATGAGGAG
CAGCACTTCTACTGGGGAGGCTCCCAGCCTGGGATCCAGCGCTGTGCCTGTGGTCTGGAC
CGGAGCTGTGTGGACCCTGCCTTGTACTGCAACTGTGACGCTGACCAGCCCCAGTGGAGA
ACTGACAAGGGACTGCTGACCTTTGTGGACCATCTGCCTGTCACTCAGGTAGTGATAGGG
GATACGAACCGCTCCACTTCTGAGGCCCAGTTCTTCCTGAGGCCTCTGCGCTGCTATGGC
GATCGAAATTCCTGGAACACCATTTCCTTCCACACCGGGGCTGCACTACGCTTCCCCCCA
ATCCGTGCCAACCACAGCCTGGATGTCTCCTTCTACTTCAGGACCTCTGCTCCCTCGGGG
GTCTTCCTAGAGAATATGGGGGGCCCTTACTGCCAGTGGCGCCGACCTTATGTGCGGGTG
GAACTCAACACATCCCGGGATGTGGTCTTCGCCTTTGATGTGGGGAATGGGGATGAGAAC
CTCACAGTACACTCAGACGACTTTGAGTTCAATGATGACGAGTGGCACCTGGTCCGGGCT
GAAATCAACGTGAAGCAGGCCCGGCTCCGAGTGGATCACCGGCCCTGGGTTCTGCGGCCT
ATGCCACTGCAGACCTACATCTGGATGGAGTATGACCAGCCCCTCTATGTGGGATCTGCA
GAGCTTAAGAGACGCCCCTTTGTGGGTTGCTTGAGGGCCATGCGTCTGAACGGAGTGACT
CTGAACCTGGAGGGCCGTGCCAATGCCTCTGAGGGTACCTCACCCAACTGCACAGGCCAC
TGTGCCCACCCTCGGCTCCCCTGTTTCCATGGAGGCCGCTGCGTGGAGCGCTATAGCTAC
TACACGTGTGACTGTGACCTCACGGCTTTTGATGGGCCATACTGCAACCACGATATTGGT
GGTTTCTTTGAGCCGGGCACCTGGATGCGCTATAACCTACAGTCAGCGCTGCGCTCTGCA
GCCAGGGAGTTCTCCCACATGCTGAGCCGGCCAGTGCCAGGCTATGAGCCTGGCTACATC
CCGGGCTATGATACTCCGGGCTATGTGCCTGGCTACCATGGCCCCGGGTACCGCCTGCCC
GACTACCCCCGGCCTGGTCGGCCTGTGCCCGGTTACCGTGGGCCTGTCTACAACGTTACG
GGAGAGGAGGTCTCCTTCAGCTTCAGCACCAGCTCCGCCCCTGCTGTCCTGCTCTACGTC
AGTTCCTTTGTTCGTGACTACATGGCTGTGCTCATCAAGGATGATGGGACCCTTCAGCTG
CGATATCAGCTGGGCACCAGTCCCTACGTGTACCAGCTAACCACTCGACCAGTGACCGAT
GGCCAGCCCCATAGCATCAATATCACCCGTGTTTACCGGAACCTCTTCATCCAGGTGGAC
TACTTCCCACTGACAGAGCAGAAGTTCTCGCTGTTGGTGGACAGCCAGTTGGACTCACCC
AAGGCCTTGTATTTAGGGCGTGTGATGGAGACAGGAGTCATTGACCCGGAGATCCAGCGC
TACAACACCCCAGGTTTCTCAGGCTGCCTGTCTGGTGTTCGATTCAACAACGTGGCTCCC
CTCAAGACCCACTTCCGAACCCCTCGACCCATGACTGCTGAGCTAGCTGAGGCCCTTCGA
GTTCAGGGAGAACTGTCCGAATCTAATTGCGGAGCTATGCCACGTCTTGTTTCAGAGGTG
CCACCTGAGCTTGATCCCTGGTATCTGCCCCCAGACTTCCCCTACTACCATGATGAAGGA
TGGGTTGCCATACTTTTAGGCTTTTTGGTGGCCTTTCTGCTGCTGGGGCTGGTGGGAATG
TTGGTGCTCTTCTATCTGCAAAATCATCGCTATAAGGGCTCCTACCATACCAATGAGCCC
AAGGCTGCCCACGAGTACCATCCTGGCAGCAAACCTCCCCTACCCACTTCAGGCCCTGCC
CAGGTCCCCACCCCTACAGCAGCTCCCAACCAAGCTCCAGCCTCAGCCCCAGCCCCAGCC
CCAACTCCAGCCCCAGCCCCTGGCCCCCGGGATCAGAACCTACCCCAGATCCTGGAGGAG
TCCAGGTCTGAATTG
Protein sequence predicted based on nucleotide sequence
MMHLRLFCILLAAVSGAEGWGYYGCDEELVGPLYARSLGASSYYSLLTAPRFARLHGISG WSPRIGDPNPWLQIDLMKKHRIRAVATQGSFNSWDWVTRYMLLYGDRVDSWTPFYQRGHN STFFGNVNESAVVRHDLHFHFTARYIRIVPLAWNPRGKIGLRLGLYGCPYKADILYFDGD DAISYRFPRGVSRSLWDVFAFSFKTEEKDGLLLHAEGAQGDYVTLELEGAHLLLHMSLGS SPIQPRPGHTTVSAGGVLNDQHWHYVRVDRFGRDVNFTLDGYVQRFILNGDFERLNLDTE MFIGGLVGAARKNLAYRHNFRGCIENVIFNRVNIADLAVRRHSRITFEGKVAFRCLDPVP HPINFGGPHNFVQVPGFPRRGRLAVSFRFRTWDLTGLLLFSRLGDGLGHVELTLSEGQVN VSIAQSGRKKLQFAAGYRLNDGFWHEVNFVAQENHAVISIDDVEGAEVRVSYPLLIRTGT SYFFGGCPKPASRWDCHSNQTAFHGCMELLKVDGQLVNLTLVEGRRLGFYAEVLFDTCGI TDRCSPNMCEHDGRCYQSWDDFICYCELTGYKGETCHTPLYKESCEAYRLSGKTSGNFTI DPDGSGPLKPFVVYCDIRENRAWTVVRHDRLWTTRVTGSSMERPFLGAIQYWNASWEEVS ALANASQHCEQWIEFSCYNSRLLNTAGGYPYSFWIGRNEEQHFYWGGSQPGIQRCACGLD RSCVDPALYCNCDADQPQWRTDKGLLTFVDHLPVTQVVIGDTNRSTSEAQFFLRPLRCYG DRNSWNTISFHTGAALRFPPIRANHSLDVSFYFRTSAPSGVFLENMGGPYCQWRRPYVRV ELNTSRDVVFAFDVGNGDENLTVHSDDFEFNDDEWHLVRAEINVKQARLRVDHRPWVLRP MPLQTYIWMEYDQPLYVGSAELKRRPFVGCLRAMRLNGVTLNLEGRANASEGTSPNCTGH CAHPRLPCFHGGRCVERYSYYTCDCDLTAFDGPYCNHDIGGFFEPGTWMRYNLQSALRSA AREFSHMLSRPVPGYEPGYIPGYDTPGYVPGYHGPGYRLPDYPRPGRPVPGYRGPVYNVT GEEVSFSFSTSSAPAVLLYVSSFVRDYMAVLIKDDGTLQLRYQLGTSPYVYQLTTRPVTD GQPHSINITRVYRNLFIQVDYFPLTEQKFSLLVDSQLDSPKALYLGRVMETGVIDPEIQR YNTPGFSGCLSGVRFNNVAPLKTHFRTPRPMTAELAEALRVQGELSESNCGAMPRLVSEV PPELDPWYLPPDFPYYHDEGWVAILLGFLVAFLLLGLVGMLVLFYLQNHRYKGSYHTNEP KAAHEYHPGSKPPLPTSGPAQVPTPTAAPNQAPASAPAPAPTPAPAPGPRDQNLPQILEE SRSEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
