Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00866757
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5820nts
Open reading frame: 1 to 5820
ATGAGTTCTGACTCTGAGATGGCCATTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCT
GAAAAGGAGCGAATTGAAGCTCAGAACAAGCCTTTTGATGCCAAGACATCAGTCTTTGTG
GTGGACCCTAAGGAGTCCTACGTGAAAGCAATAGTGCAGAGCAGGGAAGGGGGGAAGGTG
ACAGCCAAGACCGAAGCTGGAGCTACTGTAACTGTGAAAGAAGACCAAGTCTTCTCCATG
AACCCTCCCAAATATGACAAGATCGAGGACATGGCCATGATGACTCACCTGCATGAGCCT
GCTGTGCTGTATAACCTCAAAGAGCGTTACGCAGCCTGGATGATCTACACCTACTCGGGC
CTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAGGTGGTG
ACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCATATCTTCTCCATCTCTGAC
AATGCCTATCAGTTCATGCTAACTGATCGTGAAAACCAGTCAATCTTGATTACTGGAGAA
TCTGGTGCAGGGAAGACTGTGAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCA
GTTACTGGAGAGAAGAAAAAAGAGGAACCTGCCTCTGGCAAAATGCAGGGGACCCTTGAA
GATCAAATCATCAGTGCTAACCCCCTACTGGAAGCCTTCGGCAATGCCAAGACCGTGAGG
AATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATCCATTTTGGTGCCACAGGCAAA
CTGGCTTCTGCAGATATTGAAACATATCTGCTAGAGAAGTCCCGAGTTACTTTTCAGCTA
AAGGCTGAAAGAAGCTACCACATATTTTATCAAATCCTGTCCAATAAGAAACCAGAGCTC
ATTGAAATGCTTCTGATCACCACCAACCCATATGACTTCGCATTTGTCAGCCAAGGGGAA
ATTACTGTGCCCAGCATTGATGACCAGGAAGAGCTGATGGCCACAGATAGTGCTGTGGAC
ATCCTGGGTTTCACTGCTGATGAAAAGGTGGCCATTTACAAGCTCACTGGAGCCGTGATG
CATTATGGGAACATGAAATTCAAGCAAAAGCAAAGGGAAGAGCAGGCAGAGCCAGATGGC
ACGGAAGTTGCTGACAAAGCTGCTTATCTGACAAGTCTGAACTCTGCTGACCTGCTCAAA
TCTCTCTGCTATCCCAGAGTCAAGGTCGGCAATGAGTTCGTAACCAAAGGCCAGACTGTG
CAGCAGGTGTACAATGCAGTGGGTGCTCTGGCCAAAGCCATCTACGAGAAGATGTTCCTG
TGGATGGTCACCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATC
GGGGTCTTGGACATTGCTGGCTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGC
ATCAACTTCACCAACGAGAAACTGCAACAGTTTTTCAACCACCACATGTTCGTGCTGGAG
CAGGAAGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCATTGACTTCGGGATGGACCTG
GCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCTAGAAGAGGAG
TGCATGTTCCCCAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGAACAACATCTT
GGAAAATCCAACAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAGCCTGAGGCTCACTTC
TCACTGGTGCACTATGCCGGCACCGTGGACTACAACATCGCCGGCTGGCTGGACAAAAAC
AAGGACCCCCTGAATGAGACTGTGGTGGGGCTGTACCAGAAGTCTGCAATGAAGACTCTG
GCTTTCCTCTTCTCTGGGGCACAAACTGCTGAAGCAGAGGGTGGTGGTGGAAAGAAAGGT
GGCAAAAAGAAGGGTTCTTCTTTCCAGACAGTGTCAGCTCTTTTCAGGGAGAATTTGAAT
AAGCTGATGACCAACTTGAGGAGCACTCACCCCCACTTTGTGCGGTGCATCATCCCCAAT
GAAACTAAAACTCCTGGTGCCATGGAGCATGAGCTTGTCCTGCATCAGCTGAGGTGTAAC
GGTGTGCTGGAAGGCATCCGCATCTGCAGGAAAGGCTTCCCAAGCAGAATCCTTTATGCA
GACTTCAAACAGAGATACAAGGTTCTAAATGCGAGTGCTATCCCAGAGGGTCAGTTCATT
GACAGCAAGAAGGCTTCTGAGAAACTTCTAGGGTCTATTGAAATTGACCACACCCAGTAC
AAATTCGGTCATACCAAGGTTTTCTTCAAAGCTGGCCTGCTGGGAACTCTAGAGGAAATG
CGAGATGAAAAGCTAGCTCAACTCATCACGCGCACTCAAGCCATATGCAGAGGGTTCCTG
ATGAGAGTGGAGTTCAGAAAGATGATGGAGAGGAGAGAGTCCATCTTCTGCATTCAGTAC
AACATCCGTGCTTTCATGAATGTGAAGCACTGGCCCTGGATGAAGCTGTATTTCAAGATC
AAGCCCCTCCTCAAGAGTGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAAGAATTT
GAGAAAACCAAAGAAGAGCTGGCTAAGACAGAGGCAAAAAGGAAAGAACTAGAAGAAAAG
ATGGTGACGCTAATGCAAGAGAAAAATGACTTACAACTCCAAGTTCAAGCTGAAGCAGAT
GCCTTGGCTGATGCAGAGGAAAGATGTGATCAGTTGATTAAAACCAAAATCCAACTTGAG
GCCAAAATCAAAGAGGTAACTGAAAGAGCTGAGGATGAGGAAGAGATCAATGCTGAGCTG
ACAGCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAGCTCAAGAAAGACATTGATGAC
CTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAGAACAAGGTGAAA
AACCTCACAGAAGAGATGGCAGGTCTGGATGAAACCATTGCTAAGCTGACCAAGGAGAAG
AAGGCTCTCCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGATGGAGGAGGACAAA
GTCAACACCCTGACCAAAGCTAAAACCAAGCTAGAACAGCAAGTGGACGATCTTGAAGGA
TCTCTGGAACAAGAAAAGAAACTTTGCATGGACTTAGAAAGAGCCAAGAGAAAACTGGAG
GGTGACCTAAAATTGGCCCAAGAATCCACAATGGATACAGAAAATGACAAACAGCAACTT
AATGAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAATCTGCAAGGCAAGATTGAAGAT
GAACAAGCCCTTGCAATACAGCTACAAAAGAAGATCAAAGAATTACAGGCCCGCATTGAG
GAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGC
TCTGACCTCTCCCGGGAGCTGGAGGAGATCAGTGAGAGGCTGGAAGAAGCCGGTGGGGCC
ACTTCAGCCCAGATTGAGATGAACAAGAAGCGGGAGGCTGAGTTCCAGAAAATGCGCAGG
GACCTGGAAGAGTCCACCCTGCAGCACGAAGCCACGGCAGCTGCTCTTCGGAAGAAGCAC
GCAGATAGTGTGGCTGAGCTTGGGGAGCAGATTGACAGCCTTCAGCGGGTCAAGCAGAAG
CTGGAGAAGGAAAAGAGTGAGCTGAAGATGGAGATCAATGACCTTGCTAGTAACATGGAG
ACTGTCTCCAAAGCCAAGGCAAACTTTGAGAAAATGTGCCGCACCCTAGAGGACCAGCTT
AGTGAAATAAAAACAAAGGAAGAAGAGCAACAACGCTTAATAAATGAGTTGTCAGCCCAG
AAGGCACGTTTACACACAGAATCAGGTGAGTTTTCACGACAGCTAGATGAAAAAGATGCT
ATGGTTTCTCAGCTATCCCGAGGCAAACAAGCATTTACACAACAGATTGAAGAATTAAAG
AGGCAGCTAGAAGAGGAGACTAAGGCCAAGAGCACTCTGGCCCATGCCCTGCAGTCAGCC
CGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGGAGCAGGAAGCCAAGGCTGAG
CTGCAGAGGGGAATGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGACCAAGTACGAG
ACGGACGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTAGCCCAGCGT
CTGCAGGATGCAGAAGAACATGTAGAAGCTGTGAATTCCAAATGTGCTTCTCTTGAAAAG
ACAAAGCAGAGGCTACAGAATGAAGTAGAGGACCTCATGATTGATGTGGAACGATCTAAT
GCTGCCTGCATAGCTCTCGATAAGAAGCAAAGAAACTTTGACAAGGTTCTGGCAGAATGG
AAACAGAAGTATGAGGAAACTCAGGCTGAACTTGAGGCCTCCCAGAAGGAGTCGCGTTCT
CTCAGCACTGAGCTGTTCAAGGTGAAGAATGCCTACGAGGAATCCCTGGATCATCTTGAA
ACTCTAAAGCGAGAGAATAAGAACTTACAACAGGAGATTTCTGACCTGACAGAGCAAATT
GCAGAGGGTGGAAAGCATATCCATGAACTGGAGAAAGTAAAGAAACAACTTGATCATGAG
AAGAGTGAACTACAGACTTCCCTAGAGGAAGCAGAGGCATCTCTTGAGCATGAAGAAGGC
AAAATTCTTCGCATTCAACTTGAGCTAAATCAGGTGAAATCTGAGATTGACCGAAAAATT
GCTGAAAAAGATGAAGAACTCGATCAGCTAAAGAGGAACCATCTCAGAGTTGTGGAGTCA
ATGCAGAGTACACTGGATGCTGAGATCAGGAGCAGAAATGATGCTCTGAGGATCAAGAAG
AAGATGGAGGGAGATCTTAATGAAATGGAAATCCAGCTGAACCATGCCAACCGCCAGGCT
GCTGAGGCACTAAGGAATCTTAGAAACACACAAGGAATACTGAAGGACACTCAGCTACAT
TTGGATGATGCCATCAGAGGCCAAGATGACCTTAAGGAACAACTGGCAATGGTTGAGCGC
AGAGCTAACCTGATGCAGGCTGAAGTTGAAGAGCTCAGGGCATCCCTGGAACGGACTGAG
AGAGGCAGGAAAATGGCAGAGCAAGAGCTTCTGGATGCCAGTGAACGTGTGCAACTTCTG
CACACTCAGAACACCAGCCTGATCAACACCAAGAAGAAGCTGGAAACAGACATTTCCCAA
ATCCAGGGAGAGATGGAGGACATCGTCCAGGAAGCCCGCAATGCAGAGGAGAAGGCCAAG
AAGGCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGAAGAAGGAACAGGACACCAGC
GCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTGAAGGATCTGCAGCTCCGT
CTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGTGGGAAGAAGCAGATCCAGAAACTGGAG
GCCAGGGTGAGAGAGCTTGAAAGTGAGGTGGAAAGTGAACAGAAGCACAATGTTGAGGCT
GTCAAGGGTCTTCGCAAACATGAGAGAAGAGTGAAGGAACTCACTTACCAGACTGAGGAG
GACCGCAAGAATATTCTCAGGCTGCAGGACTTGGTGGACAAATTGCAAACCAAAGTCAAA
GCTTACAAGAGACAAGCTGAAGAGGCTGAGGAACAATCCAATGTCAACCTTGCCAAGTTC
CGCAAGCTCCAGCACGAGCTGGAGGAGGCCAAGGAACGGGCTGACATTGCTGAGTCCCAA
GTCAACAAGCTGAGAGTGAAGAGTCGGGAGGTTCACACAAAAGTCATAAGTGAAGAGTTG
Protein sequence predicted based on nucleotide sequence
MSSDSEMAIFGEAAPFLRKSEKERIEAQNKPFDAKTSVFVVDPKESYVKAIVQSREGGKV TAKTEAGATVTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSG LFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGE SGAGKTVNTKRVIQYFATIAVTGEKKKEEPASGKMQGTLEDQIISANPLLEAFGNAKTVR NDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPEL IEMLLITTNPYDFAFVSQGEITVPSIDDQEELMATDSAVDILGFTADEKVAIYKLTGAVM HYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKSLCYPRVKVGNEFVTKGQTV QQVYNAVGALAKAIYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLC INFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEE CMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLVHYAGTVDYNIAGWLDKN KDPLNETVVGLYQKSAMKTLAFLFSGAQTAEAEGGGGKKGGKKKGSSFQTVSALFRENLN KLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYA DFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIEIDHTQYKFGHTKVFFKAGLLGTLEEM RDEKLAQLITRTQAICRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKI KPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEAD ALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDD LELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDK VNTLTKAKTKLEQQVDDLEGSLEQEKKLCMDLERAKRKLEGDLKLAQESTMDTENDKQQL NEKLKKKEFEMSNLQGKIEDEQALAIQLQKKIKELQARIEELEEEIEAERASRAKAEKQR SDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEESTLQHEATAAALRKKH ADSVAELGEQIDSLQRVKQKLEKEKSELKMEINDLASNMETVSKAKANFEKMCRTLEDQL SEIKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELK RQLEEETKAKSTLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTKYE TDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSN AACIALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDHLE TLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAEASLEHEEG KILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVESMQSTLDAEIRSRNDALRIKK KMEGDLNEMEIQLNHANRQAAEALRNLRNTQGILKDTQLHLDDAIRGQDDLKEQLAMVER RANLMQAEVEELRASLERTERGRKMAEQELLDASERVQLLHTQNTSLINTKKKLETDISQ IQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLR LDEAEQLALKGGKKQIQKLEARVRELESEVESEQKHNVEAVKGLRKHERRVKELTYQTEE DRKNILRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKFRKLQHELEEAKERADIAESQ VNKLRVKSREVHTKVISEEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
