Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00867405
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 9693nts
Open reading frame: 1 to 9693
ATGCAGAGCAGCCCCTCCCCTGCTCACCCTCAGCTCCCAGTCCTACAGACACAGATGGTG
TCGGACGGCATGACAGGCAGCAATCCTGTGTCCCCTGCCTCATCCAGTTCCCCAGCCTCT
AGTGGGGCAGGCGGCATCTCCCCGCAGCACATAGCTCAAGATTCCTCACTGGATGGACCT
CCAGGCCCCCCAGATGGTGCCACAGTGCCCCTGGAGGGGTTCAGCTTATCCCAGGCTGCT
GACCTGGCTAACAAGGGCCCGAAGTGGGAGAAGAGCCATGCCGAAATTGCAGAACAGGCC
AAGCATGAGGCCGAGATCGAGACTCGGATTGCTGAGCTGCGGAAGGAGGGTTTCTGGTCA
CTGAAGAGGCTGCCTAAGGTGCCAGAGCCCCCTCGCCCCAAAGGTCACTGGGACTATTTG
TGCGAAGAGATGCAGTGGCTCTCTGCTGACTTTGCTCAGGAGCGCCGTTGGAAACGGGGT
GTGGCCCGGAAGGTGGTGCGCATGGTGATCCGGCACCACGAGGAGCAGCGGCAGAAAGAG
GAACGGGCCCGGAGGGAGGAGCAGGCCAAGCTGCGTCGAATTGCTTCCACCATGGCCAAG
GATGTCAGGCAGTTCTGGAGCAATGTGGAGAAGGTGGTGCAATTCAAGCAACAGTCCCGG
CTTGAGGAAAAGCGCAAAAAAGCCCTGGACCTGCATTTGGACTTCATTGTGGGGCAAACT
GAAAAGTACTCGGACCTTCTGTCTCAGAGCCTCAACCAGCCATTAACCTCCAGCAAAGCA
GGCTCTTCCCCTTGCCTCGGCTCTTCCTCAGCTGCCTCCAGTCCTCCACCCCCTGCTTCT
CGCCTGGATGATGAAGATGGGGACTTTCAACCCCAAGAGGATGAGGAAGAGGATGATGAG
GAAACGATTGAAGTTGAAGAACAACAGGAAGGCAATGATGCAGAGGCCCAGAGGCGTGAG
ATTGAGCTGCTTCGCCGTGAGGGAGAATTGCCACTGGAAGAGCTGCTCCGTTCCCTTCCC
CCTCAGCTGTTGGAAGGGCCTTCCAGCCCCTCTCAAACCCCCTCATCTCATGATAGTGAC
ACCCGAGATGGGCCTGAAGAAGGTGCTGAAGAAGAGCCCCCTCAGGTGTTGGAGATAAAG
CCCCCACCCTCTGCTGTCACACAGCGCAACAAACAGCCTTGGCATCCAGATGAAGATGAT
GAAGAGTTTACTGCCAACGAAGAGGAAGCGGAGGATGAAGAGGATACTATAGCAGCTGAG
GAACAGTTGGAAGGGGAGGTGGATCATGCCATGGAGCTGAGCGAGTTGGCTCGAGAAGGT
GAGCTTTCCATGGAGGAGCTATTGCAGCAGTATGCAGGAGCCTATGCCCCAGGCTCTGGG
AGCAGTGAAGATGAGGATGAAGATGAGGTTGATGCTAATAGCTCTGACTGTGAACCAGAG
GGGCCCGTGGAAGCGGAAGAGCCTCCTCAGGAGGATAGTAGCAGTCAGTCAGACTCTGTG
GAGGACCGGAGTGAGGATGAGGAAGATGAACATTCAGAGGAGGAAGAAACAAGTGGAAGT
TCAGCATCAGAGGAATCTGAGTCTGAAGAGTCTGAGGATGCCCAATCACAGAGCCAAGCA
GATGAAGAGGAGGAAGATGATGATTTTGGGGTGGAGTACTTGCTTGCCAGGGATGAAGAG
CAGAGTGAGGCAGATGCAGGCAGTGGGCCTCCTACTCCAGGGCCCACTACTCTAGGTCCA
AAGAAAGAAATTACTGACATTGCTGCAGCAGCTGAAAGTCTCCAGCCCAAGGGTTACACG
CTGGCCACGACCCAGGTAAAGACGCCCATTCCCCTGCTTCTGCGGGGCCAGCTCCGGGAG
TACCAGCACATTGGGCTAGACTGGCTGGTTACCATGTATGAGAAGAAGCTTAATGGCATT
CTTGCTGATGAGATGGGGCTTGGGAAGACCATCCAGACCATCTCTCTGCTTGCCCACTTG
GCTTGTGAGAAAGGTAACTGGGGTCCCCATTTAATCATTGTTCCCACCAGCGTGATGTTG
AACTGGGAGATGGAGTTGAAACGTTGGTGCCCCAGCTTTAAAATCCTCACTTACTATGGA
GCCCAGAAAGAGAGGAAGCTCAAGCGGCAGGGCTGGACCAAGCCCAATGCCTTTCATGTG
TGTATCACATCTTACAAGCTGGTGCTGCAGGACCACCAGGCCTTCCGTCGCAAGAACTGG
CGCTATCTCATTCTGGATGAGGCGCAGAACATCAAGAACTTCAAGTCACAGCGCTGGCAG
TCACTCCTCAACTTCAACAGCCAGAGACGCCTGCTCCTGACAGGAACTCCCTTGCAGAAC
AGCCTCATGGAGCTGTGGTCCTTGATGCACTTTTTGATGCCCCATGTCTTCCAGTCTCAT
CGCGAGTTCAAGGAGTGGTTCTCTAATCCCCTAACTGGCATGATTGAGGGCAGCCAAGAG
TATAATGAAGGTCTAGTCAAACGCCTCCACAAGGTTTTGAGGCCTTTTTTACTGCGCCGA
GTTAAGGTGGATGTTGAGAAGCAGATGCCCAAAAAGTACGAGCATGTTATCCGCTGCAGG
CTCTCCAAGCGTCAACGCTGTCTCTATGATGACTTCATGGCACAGACCACAACTAAGGAG
ACACTAGCCACAGGCCATTTCATGAGCGTCATCAACATTTTGATGCAGCTGAGAAAAGTT
TGCAATCATCCAAATCTGTTCGACCCTCGACCGGTTACCTCCCCTTTCATCACCCCAGGC
ATCTGCTTCAGCACCGCCTCTCTGGTGCTAAGGGCCACGGATGTCCATCCCCTCCAGCGG
ATAGACATGGGTCGATTTGACCTTATTGGCCTGGAAGGTCGTGTCTCTCGATATGAGGCA
GACACATTTCTGCCCCGGCACCGCCTCTCTCGCCGGGTACTGTTAGAAGTGGCTACTGCT
CCTGACCCCCCACCCCGGCCCAAGCCAGTCAAGATGAAGGTCAACAGGATGCTGCAGCCA
GTACCTAAGCAAGAAGGCCGGACAGTGGTGGTGGTGAACAACCCACGGGCGCCCCTGGGC
CCTGTCCCAGTTCGACCTCCTCCAGGTCCTGAGCTCTCAGCCCAGCCCACCCCTGGCCCA
GTCCCCCAAGTGCTGCCAGCATCACTGATGGTTTCAGCCTCACCTGCCGGGCCCCCGCTT
ATTCCTGCATCTCGGCCTCCTGGCCCTGTCCTCTTGCCTCCACTGCAGCCCAACAGTGGT
TCTCTCCCCCAGGTGTTGCCATCCCCCCTGGGGGTCCTGAGTGGGACCTCACGGCCTCCC
ACGCCAACCTTGTCCCTAAAGCCAACACCACCTGCCCCAGTTCGCCTGAGCCCAGCCCCA
CCTCCAGGCTCCTCTAGCCTGTTGAAGCCCCTGACAGTGCCACCAGGCTACACCTTCCCT
CCTGCTGCTGCCACCACCACTTCTACCACCACGGCAACTGCTACCACCACAGCAGTGCCA
GCTCCGACTCCTGCACCACAGCGCCTCATTCTATCTCCCGATATGCAGGCTCGCCTGCCC
TCAGGCGAAGTGGTCAGCATCGGGCAGTTAGCCTCACTGGCACAACGTCCAGTGGCTAAT
GCAGGGGGAAGCAAACCTCTCACCTTCCAAATCCAGGGCAACAAGCTGACTTTGACTGGT
GCCCAGGTGCGCCAGCTTGCTGTGGGGCAGCCCCGCCCGCTGCAAAGGAATGTGGTGCAC
CTCGTGTCAGCAGGGGGGCAGCACCATCTCATCAGCCAGCCTGCCCATGTGGCCCTCATC
CAGGCCGTGGCCCCGACCCCTGGCCCTACCCCTGTCTCTGTGCTGCCTTCTTCGACCCCC
AGCACCACCCCTGCCCCTACTGGCCTCAGCCTTCCGCTTGCTGCTAACCAGGTGCCACCA
ACCATGGTGAATAATACAGGCGTGGTGAAGATTGTAGTGAGACAAGCCCCTCGGGATGGA
CTGACTCCTGTTCCTCCATTGGCCCCAGCACCCCGGCCTCCGAGCTCTGGGCTTCCAGCT
GTGTTGAATCCACGCCCCACGTTAACCCCTGGCCGGCTACCCACACCTACTCTGGGTACT
GCTCGAGCCCCCATGCCCACACCCACTCTGGTGAGGCCTCTTCTCAAGCTGGTCCACAGT
CCTTCACCTGAAGTCAGTGCTTCAGCCCCCGGAGCTGCCCCCTTGACCATCTCTTCTCCT
CTCCACGTGCCATCCTCACTCCCTGGGCCAGCCTCTTCTCCAATGCCAATTCCCAACTCC
TCTCCCCTTGCTAGTCCTGTGTCCTCTACAGTCTCAGTTCCATTGTCATCTTCACTCCCC
ATCTCTGTCCCCACCACACTTCCTGCCCCAGCCTCGGCTCCACTCACCATCCCCATCTCA
GCCCCCTTGACTGTTTCTGCTTCGGGCCCAGCTCTGTTGACCAGTGTGACTCCACCATTG
GCACCTGTTGTCCCAGCGGCTCCTGGACCTCCCTCCTTGGCACCATCTGGTGCTTCCCCG
TCAGCATCAGCCTTGACTCTAGGTTTGGCCACAGCTCCATCCCTGTCTTCATCTCAGACA
CCTGGTCACCCTCTGTTGTTGGCTCCCACCTCTTCACATGTTCCAGGGTTGAACTCAACC
GTGGCCCCAGCATGCTCACCTGTCCTGGTGCCAGCTTCGGCTCTGGCCAGTCCTTTTCCG
TCAGCACCAAATCCAGCTCCAGCTCAGGCTTCCCTTCTGGCTCCAGCATCTTCTGCATCT
CAGGCTCTAGCCACCCCTCTGGCTCCTATGGCGGCTCCACAGACAGCAATTCTGGCTCCT
TCTCCAGCTCCTCCTCTGGCTCCTCTTCCGGTCCTGGCACCATCGCCAGGTGCTGCTCCT
GTCCTGGCTTCATCACAGACTCCGGTTCCAGTTATGGCTCCATCGTCTACTCCAGGAACC
TCTTTAGCCTCAGCTTCACCGGTACCAGCTCCAACCCCTGTGTTGGCTCCATCATCAACT
CAAACTATGCTACCAGCCCCGGTTCCGTCACCTCTCCCGAGCCCGGCTTCTACGCAGACA
CTGGCCCTAGCCCCAGCTTTAGCACCCACTCTTGGAGGCTCATCTCCATCTCAGACACTC
TCTTTGGGAACGGGGAACCCCCAGGGACCCTTTCCAACTCAGACATTGTCATTAACTCCA
GCATCATCCCTGGTACCAACTCCAGCCCAGACACTGTCTTTGGCACCAGGACCACCACTG
GGTCCAACTCAGACGCTGTCTCTGGCTCCAGCACCCCCTCTGGCTCCAGCTTCTCCAGTG
GGCCCAGCCCCAGCTCACACGCTGACTTTGGCTCCAGCATCGTCATCTGCTTCACTCCTG
GCCCCAGCTTCAGTGCAGACACTGACCTTGAGCCCTGCCCCAGTTCCTACCCTGGGCCCG
GCCGCAGCTCAGACCTTGGCGCTGGCCCCAGCCTCCACACAGTCCCCAGCTTCCCAGGCA
TCTTCCCTTGTGGTTTCGGCATCTGGTGCCGCTCCCTTGCCTGTCACCATGGTATCCCGG
CTGCCTGTTTCCAAGGATGAGCCTGACACACTGACATTGCGCTCTGGTCCCCCCAGCCCT
CCCTCCACTGCTACCTCGTTTGGTGGCCCCCGGCCTCGACGCCAGCCCCCCCCACCACCT
CGTTCCCCTTTTTATCTGGACTCCCTGGAGGAAAAGCGGAAGCGGCAGCGGTCTGAACGC
CTGGAACGGATTTTCCAACTTAGTGAGGCTCATGGGGCCCTGGCACCTGTGTATGGGACT
GAAGTCCTGGATTTCTGTACCCTGCCCCAACCTGTTGCCAGCCCCATCGGCCCTCGTTCT
CCTGGCCCCAGCCACCCCACCTTTTGGACTTATACCGAGGCTGCCCACCGGGCTGTACTG
TTTCCCCAGCAGCGACTAGACCAGCTGTCAGAAATCATTGAGAGGTTCATCTTTGTCATG
CCTCCTGTGGAGGCACCTCCCCCTTCCCTGCATGCCTGCCACCCACCTCCTTGGCTGGCC
CCACGTCAGGCAGCCTTCCAGGAGCAATTGGCCTCTGAGCTCTGGCCCCGGGCTCGTCCT
TTGCACCGTATTGTGTGTAACATGCGCACCCAGTTCCCTGACTTAAGACTCATCCAGTAT
GATTGCGGAAAGTTGCAGACGTTGGCAGTGCTGTTGCGGCAGCTCAAGGCAGAGGGCCAC
CGAGTGCTCATCTTCACCCAGATGACCCGAATGCTGGATGTATTGGAGCAGTTTCTCACC
TACCATGGCCATCTCTACCTGCGCCTGGATGGATCTACTAGAGTTGAACAGAGACAGGCC
TTGATGGAACGGTTCAATGCAGACAAACGCATATTCTGCTTCATCCTTTCAACTCGGAGT
GGGGGTGTGGGCGTGAACCTGACAGGAGCAGACACTGTTGTTTTTTATGACAGCGACTGG
AATCCCACCATGGATGCTCAGGCCCAGGACCGCTGTCACCGAATTGGCCAGACCCGGGAT
GTCCACATATATAGGCTTATCAGTGAACGGACAGTGGAGGAGAACATCCTAAAAAAGGCA
AATCAGAAGAGAATGTTGGGGGACATGGCCATTGAGGGAGGCAACTTCACCACAGCCTAT
TTCAAACAGCAGACCATCCGAGAGCTGTTTGATATGCCCCTGGAGGAACCTTCTAGCTCA
TCCGTGCCCTCTGCCCCTGAAGAGGAGGAAGAGACTGTGGCCAGCAAGCAGACTCATATT
CTGGAGCAGGCATTGTGTCGGGCAGAAGATGAAGAGGATATCCGTGCAGCCACCCAGGCC
AAGGCTGAACAGGTGGCTGAGCTTGCAGAATTTAATGAGAACGATGGGTTTCCTGCTGGT
GAGGGAGAGGAAGCTGGCCGGCCTGGGGCTGAGGATGAGGAGATGTCCCGGGCTGAGCAG
GAAATTGCTGCCCTCGTAGAACAGCTGACCCCCATTGAGCGCTATGCCATGAAATTCCTG
GAGGCCTCACTGGAGGAGGTGAGCCGAGAGGAGCTCAAACAGGCAGAAGAGCAAGTGGAA
GCTGCCCGCAAAGACCTGGACCAAGCCAAGGAGGAGGTGTTCCGCCTACCCCAAGAGGAG
GAGGAGGGGCCGGGGGCTGGGGATGAGAGTTCCTGTGGGACTGGTGGAGGCACCCACCGG
CGCAGTAAAAAGGCCAAAGCCCCTGAGAGGCCGGGGACTCGTGTCAGTGAGCGTCTTCGT
GGAGCCCGGGCTGAGACTCAAGGGGCAAACCACACTCCTGTCATATCCGCCCATCAAACT
CGCAGCACCACCACACCACCCCGCTGCAGTCCTGCCAGGGAGCGAGTTCCCAGGCCAGCA
CCTAGGCCTCGACCCACTCCAGCTTCAGCTCCGGCTGCAATTCCTGCCCTTGTTCCTGTC
CCAGTTTCTGCCCCAGTACCCATTTCAGCCCCAAATCCAATAACCATTCTCCCTGTCCAT
ATCTTGCCTTCTCCTCCCCCTCCTTCACAGATTCCTCCTTGTTCTTCTCCTGCCTGCACC
CCTCCTCCTGCCTGTACCCCTCCACCAGCTCATACACCGCCTCCAGCCCAAACCTGTCTT
GTAACTCCTTCCTCTCCTCTCTTGCTTGGTCCACCTTCTGTGCCCATCTCTGCCTCAGTC
ACTAATCTCCCCTTGGGCTTGAGGCCTGAGGCAGAGCTGTGTGCCCAGGCATTGGCATCT
CCAGAGTCCCTGGAGCTGGCTTCTGTGGCCAGTTCAGAAACCTCCTCACTTTCTCTTGTG
CCCCCTAAAGATCTGTTGCCAGTTGCTGTGGAGATCCTGCCTGTGTCAGAGAAGAACCTT
TCTCTCACCCCTTCTGCACCCAGCCTGACCTTGGAGGCTGGCAGCATCCCCAATGGTCAA
GAGCAGGAGGCACCAGATTCTGCTGAGGGGACCACCCTTACAGTGCTGCCTGAAGGTGAG
GAGTTGCCCCTGTGTGTGAGTGAGAGCAATGGCCTGGAGCTCCCACCCTCAGCAGCATCT
GATGAGCCACTTCAGGAGCCACTGGAGGCTGACAGGACCTCGGAAGAGCTGACAGAGGCC
AAGACCCCAACCTCCAGCCCAGAGAAGCCACAGGAACTCGTTACAGCTGAGGTTGCAGCT
CCATCCACCTCATCTTCAGCCACTTCCTCGCCTGAGGGTCCTTCACCTGCCCGACCTCCT
CGGCGTCGCACCAGTGCTGATGTGGAAATTAGGGGTCAAGGGACTGGTCGGCCAGGACAA
CCACCAGGCCCCAAAGTGCTTCGAAAGCTGCCAGGACGGCTGGTAACTGTGGTAGAGGAA
AAGGAACTGGTGCGGCGGCGGCGGCAGCAGCGGGGAGCTGCCAGCACCCTAGTGCCTGGG
GTCTCTGAGACTAGTGCCAGCCCGGGAAGCCCGTCTGTCCGCAGCATGTCAGGGCCAGAA
TCCTCCCCTCCCATTGGTGGGCCCTGTGAAGCTGCTCCTTCATCCTCACTGCCCACTCCA
CCCCAGCAGCCCTTCATTGCTCGCCGTCACATTGAGCTGGGGGTGACTGGTGGTGGCAGC
CCCGAGAATGGAGACGGAGCACTGCTCGCCATCACCCCACCTGCTGTGAAACGTCGGAGG
GGGAGGCCCCCCAAGAAGAACAGGTCTCCAGCAGATGCTGGGAGAGGTGTGGATGAGGCA
CCCTCATCCACCTTGAAGGGAAAAACCAATGGGGCTGACCCAGTCCCTGGGCCTGAGACC
CTAATTGTTGCAGATCCTGTCCTGGAACCACAGCTTATTCCTGGGCCCCAGCCTCTTGGA
CCCCAGCCAGTTCACAGACCCAATCCCCTCCTGTCACCTGTGGAGAAAAGAAGGCGAGGA
CGACCCCCTAAAGCACGAGATTTGCCCATCCCTGGGACCATTTCCTCTGCAGGGGATGGC
AACTCCGAAAGTCGGACACAGCCACCCCCACACCCATCACCCCTAACCCCACTCCCACCA
CTGCTAGTTTGTCCCACTGCTACTGTTGCCAACACTGTCACCACTGTCACCATTTCAACG
TCCCCACCCAAACGGAAGAGGGGCCGACCTCCCAAGAATCCTCCATCACCTCGGCCCAGC
CAGCTCCCCGTCTTGGACCGTGACAGCACTTCTGTTCTCGAGAGCTGTGGATTGGGGAGG
CGACGGCAACCCCAGGGCCAAGGGGAGAGTGAGGGTAGTTCCTCTGATGAGGATGGAAGC
CGCCCCCTCACCCGCCTGGCCCGCCTTCGGCTTGAAGCAGAAGGAATGCGAGGACGGAAG
AGTGGAGGGTCCATGGTGGTGGCTGTAATTCAGGATGACCTGGACTTAGCAGATAGCGGG
CCAGGCGGGTTGGAATTGACACCACCTGTGGTCTCACTAACCCCAAAACTGCGCTCGACC
CGGCTGCGTCCAGGGTCTCTAGTCCCCCCACTAGAGACTGAGAAGTTGCCTCGCAAACGA
GCAGGGGCCCCAGTTGGTGGGAGTCCTGGGCTGGCAAAGCGGGGCCGCCTACAGCCCCCA
AGTCCCCTGGGGCCTGAGGGTTCAGTAGAGGAGTCTGAGGCTGAAGCCTCAGGTGAGGAG
GAGGAAGGGGATGGGACCCCACGCCGACGTCCTGGCCCCCGCCGGCTTGTTGGGACCACC
AACCAAGGGGACCAGCGCATCCTGCGCAGCAGCGCCCCTCCCTCCCTGGCTGGCCCTGCT
GTTAGTCACAGAGGCCGCAAGGCCAAGACGTTG
Protein sequence predicted based on nucleotide sequence
MQSSPSPAHPQLPVLQTQMVSDGMTGSNPVSPASSSSPASSGAGGISPQHIAQDSSLDGP PGPPDGATVPLEGFSLSQAADLANKGPKWEKSHAEIAEQAKHEAEIETRIAELRKEGFWS LKRLPKVPEPPRPKGHWDYLCEEMQWLSADFAQERRWKRGVARKVVRMVIRHHEEQRQKE ERARREEQAKLRRIASTMAKDVRQFWSNVEKVVQFKQQSRLEEKRKKALDLHLDFIVGQT EKYSDLLSQSLNQPLTSSKAGSSPCLGSSSAASSPPPPASRLDDEDGDFQPQEDEEEDDE ETIEVEEQQEGNDAEAQRREIELLRREGELPLEELLRSLPPQLLEGPSSPSQTPSSHDSD TRDGPEEGAEEEPPQVLEIKPPPSAVTQRNKQPWHPDEDDEEFTANEEEAEDEEDTIAAE EQLEGEVDHAMELSELAREGELSMEELLQQYAGAYAPGSGSSEDEDEDEVDANSSDCEPE GPVEAEEPPQEDSSSQSDSVEDRSEDEEDEHSEEEETSGSSASEESESEESEDAQSQSQA DEEEEDDDFGVEYLLARDEEQSEADAGSGPPTPGPTTLGPKKEITDIAAAAESLQPKGYT LATTQVKTPIPLLLRGQLREYQHIGLDWLVTMYEKKLNGILADEMGLGKTIQTISLLAHL ACEKGNWGPHLIIVPTSVMLNWEMELKRWCPSFKILTYYGAQKERKLKRQGWTKPNAFHV CITSYKLVLQDHQAFRRKNWRYLILDEAQNIKNFKSQRWQSLLNFNSQRRLLLTGTPLQN SLMELWSLMHFLMPHVFQSHREFKEWFSNPLTGMIEGSQEYNEGLVKRLHKVLRPFLLRR VKVDVEKQMPKKYEHVIRCRLSKRQRCLYDDFMAQTTTKETLATGHFMSVINILMQLRKV CNHPNLFDPRPVTSPFITPGICFSTASLVLRATDVHPLQRIDMGRFDLIGLEGRVSRYEA DTFLPRHRLSRRVLLEVATAPDPPPRPKPVKMKVNRMLQPVPKQEGRTVVVVNNPRAPLG PVPVRPPPGPELSAQPTPGPVPQVLPASLMVSASPAGPPLIPASRPPGPVLLPPLQPNSG SLPQVLPSPLGVLSGTSRPPTPTLSLKPTPPAPVRLSPAPPPGSSSLLKPLTVPPGYTFP PAAATTTSTTTATATTTAVPAPTPAPQRLILSPDMQARLPSGEVVSIGQLASLAQRPVAN AGGSKPLTFQIQGNKLTLTGAQVRQLAVGQPRPLQRNVVHLVSAGGQHHLISQPAHVALI QAVAPTPGPTPVSVLPSSTPSTTPAPTGLSLPLAANQVPPTMVNNTGVVKIVVRQAPRDG LTPVPPLAPAPRPPSSGLPAVLNPRPTLTPGRLPTPTLGTARAPMPTPTLVRPLLKLVHS PSPEVSASAPGAAPLTISSPLHVPSSLPGPASSPMPIPNSSPLASPVSSTVSVPLSSSLP ISVPTTLPAPASAPLTIPISAPLTVSASGPALLTSVTPPLAPVVPAAPGPPSLAPSGASP SASALTLGLATAPSLSSSQTPGHPLLLAPTSSHVPGLNSTVAPACSPVLVPASALASPFP SAPNPAPAQASLLAPASSASQALATPLAPMAAPQTAILAPSPAPPLAPLPVLAPSPGAAP VLASSQTPVPVMAPSSTPGTSLASASPVPAPTPVLAPSSTQTMLPAPVPSPLPSPASTQT LALAPALAPTLGGSSPSQTLSLGTGNPQGPFPTQTLSLTPASSLVPTPAQTLSLAPGPPL GPTQTLSLAPAPPLAPASPVGPAPAHTLTLAPASSSASLLAPASVQTLTLSPAPVPTLGP AAAQTLALAPASTQSPASQASSLVVSASGAAPLPVTMVSRLPVSKDEPDTLTLRSGPPSP PSTATSFGGPRPRRQPPPPPRSPFYLDSLEEKRKRQRSERLERIFQLSEAHGALAPVYGT EVLDFCTLPQPVASPIGPRSPGPSHPTFWTYTEAAHRAVLFPQQRLDQLSEIIERFIFVM PPVEAPPPSLHACHPPPWLAPRQAAFQEQLASELWPRARPLHRIVCNMRTQFPDLRLIQY DCGKLQTLAVLLRQLKAEGHRVLIFTQMTRMLDVLEQFLTYHGHLYLRLDGSTRVEQRQA LMERFNADKRIFCFILSTRSGGVGVNLTGADTVVFYDSDWNPTMDAQAQDRCHRIGQTRD VHIYRLISERTVEENILKKANQKRMLGDMAIEGGNFTTAYFKQQTIRELFDMPLEEPSSS SVPSAPEEEEETVASKQTHILEQALCRAEDEEDIRAATQAKAEQVAELAEFNENDGFPAG EGEEAGRPGAEDEEMSRAEQEIAALVEQLTPIERYAMKFLEASLEEVSREELKQAEEQVE AARKDLDQAKEEVFRLPQEEEEGPGAGDESSCGTGGGTHRRSKKAKAPERPGTRVSERLR GARAETQGANHTPVISAHQTRSTTTPPRCSPARERVPRPAPRPRPTPASAPAAIPALVPV PVSAPVPISAPNPITILPVHILPSPPPPSQIPPCSSPACTPPPACTPPPAHTPPPAQTCL VTPSSPLLLGPPSVPISASVTNLPLGLRPEAELCAQALASPESLELASVASSETSSLSLV PPKDLLPVAVEILPVSEKNLSLTPSAPSLTLEAGSIPNGQEQEAPDSAEGTTLTVLPEGE ELPLCVSESNGLELPPSAASDEPLQEPLEADRTSEELTEAKTPTSSPEKPQELVTAEVAA PSTSSSATSSPEGPSPARPPRRRTSADVEIRGQGTGRPGQPPGPKVLRKLPGRLVTVVEE KELVRRRRQQRGAASTLVPGVSETSASPGSPSVRSMSGPESSPPIGGPCEAAPSSSLPTP PQQPFIARRHIELGVTGGGSPENGDGALLAITPPAVKRRRGRPPKKNRSPADAGRGVDEA PSSTLKGKTNGADPVPGPETLIVADPVLEPQLIPGPQPLGPQPVHRPNPLLSPVEKRRRG RPPKARDLPIPGTISSAGDGNSESRTQPPPHPSPLTPLPPLLVCPTATVANTVTTVTIST SPPKRKRGRPPKNPPSPRPSQLPVLDRDSTSVLESCGLGRRRQPQGQGESEGSSSDEDGS RPLTRLARLRLEAEGMRGRKSGGSMVVAVIQDDLDLADSGPGGLELTPPVVSLTPKLRST RLRPGSLVPPLETEKLPRKRAGAPVGGSPGLAKRGRLQPPSPLGPEGSVEESEAEASGEE EEGDGTPRRRPGPRRLVGTTNQGDQRILRSSAPPSLAGPAVSHRGRKAKTL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
