Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00867705
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5289nts
Open reading frame: 1 to 5289
ATGGAATGCTGCCGTCGGGCAACTCCTGGCACACTGCTCCTCTTTCTGGCTTTCCTGCTC
CTGAGTTCCAGGACCGCACGCTCCGAGGAGGACCGGGACGGCCTATGGGATGCCTGGGGC
CCATGGAGTGAATGCTCACGCACCTGCGGGGGTGGGGCCTCCTACTCTCTGAGGCGCTGC
CTGAGCAGCAAGAGCTGTGAAGGAAGAAATATCCGATACAGAACATGCAGTAATGTGGAC
TGCCCACCAGAAGCAGGTGATTTCCGAGCTCAGCAATGCTCAGCTCATAATGATGTCAAG
CACCATGGCCAGTTTTATGAATGGCTTCCTGTGTCTAATGACCCTGACAACCCATGTTCA
CTCAAGTGCCAAGCCAAAGGAACAACCCTGGTTGTTGAACTAGCACCTAAGGTCTTAGAT
GGTACGCGTTGCTATACAGAATCTTTGGATATGTGCATCAGTGGTTTATGCCAAATTGTT
GGCTGCGATCACCAGCTGGGAAGCACCGTCAAGGAAGATAACTGTGGGGTCTGCAACGGA
GATGGGTCCACCTGCCGGCTGGTCCGAGGGCAGTATAAATCCCAGCTCTCCGCAACCAAA
TCGGATGATACTGTGGTTGCAATTCCCTATGGAAGTAGACATATTCGCCTTGTCTTAAAA
GGTCCTGATCACTTATATCTGGAAACCAAAACCCTCCAGGGGACTAAAGGTGAAAACAGT
CTCAGCTCCACAGGAACTTTCCTTGTGGACAATTCTAGTGTGGACTTCCAGAAATTTCCA
GACAAAGAGATACTGAGAATGGCTGGACCACTCACAGCAGATTTCATTGTCAAGATTCGT
AACTCGGGCTCCGCTGACAGTACAGTCCAGTTCATCTTCTATCAACCCATCATCCACCGA
TGGAGGGAGACGGATTTCTTTCCTTGCTCAGCAACCTGTGGAGGAGGTTATCAGCTGACA
TCGGCTGAGTGCTACGATCTGAGGAGCAACCGTGTGGTTGCTGACCAATACTGTCACTAT
TACCCAGAGAACATCAAACCCAAACCCAAGCTTCAGGAGTGCAACTTGGATCCTTGTCCA
GCCAGTGACGGATACAAGCAGATCATGCCTTATGACCTCTACCATCCCCTTCCTCGGTGG
GAGGCCACCCCATGGACCGCGTGCTCCTCCTCGTGTGGGGGGGGCATCCAGAGCCGGGCA
GTTTCCTGTGTGGAGGAGGACATCCAGGGGCATGTCACTTCAGTGGAAGAGTGGAAATGC
ATGTACACCCCTAAGATGCCCATCGCGCAGCCCTGCAACATTTTTGACTGCCCTAAATGG
CTGGCACAGGAGTGGTCTCCGTGCACAGTGACATGTGGCCAGGGCCTCAGATACCGTGTG
GTCCTCTGCATCGACCATCGAGGAATGCACACAGGAGGCTGTAGCCCAAAAACAAAGCCC
CACATAAAAGAGGAATGCATCGTACCCACTCCCTGCTATAAACCCAAAGAGAAACTTCCA
GTCGAGGCCAAGTTGCCATGGTTCAAACAAGCTCAAGAGCTAGAAGAAGGAGCTGCTGTG
TCAGAGGAGCCCTCGTTCATCCCAGAGGCCTGGTCGGCCTGCACAGTCACCTGTGGTGTG
GGGACCCAGGTGCGAATAGTCAGGTGCCAGGTGCTCCTGTCTTTCTCTCAGTCCGTGGCT
GACCTGCCTATTGACGAGTGTGAAGGGCCCAAGCCAGCATCCCAGCGTGCCTGTTATGCA
GGCCCATGCAGCGGGGAAATTCCTGAGTTCAACCCAGACGAGACAGATGGGCTCTTTGGT
GGCCTGCAGGATTTCGACGAGCTGTATGACTGGGAGTATGAGGGGTTCACCAAGTGCTCC
GAGTCCTGTGGAGGAGGTGTCCAGGAGGCTGTGGTGAGCTGCTTGAACAAACAGACTCGG
GAGCCTGCTGAGGAGAACCTGTGCGTGACCAGCCGCCGGCCCCCACAGCTCCTGAAGTCC
TGCAATTTGGATCCCTGCCCAGCAAGGTGGGAAATTGGCAAGTGGAGTCCATGTAGTCTC
ACATGTGGGGTCGGCCTACAGACCAGAGACGTCTTCTGCAGCCACCTGCTTTCCAGAGAG
ATGAATGAAACAGTCATCCTGGCTGATGAGCTGTGTCGCCAGCCCAAGCCCAGCACGGTG
CAAGCTTGTAACCGCTTTAATTGCCCCCCAGCCTGGTACCCTGCACAGTGGCAGCCGTGT
TCCAGAACGTGTGGCGGGGGTGTTCAGAAACGTGAGGTTCTTTGCAAGCAGCGCATGGCT
GATGGCAGCTTCCTGGAGCTTCCTGAGACCTTCTGTTCAGCTTCAAAACCTGCCTGCCAG
CAAGCATGCAAGAAAGATGACTGTCCCAGCGAGTGGCTTCTCTCAGACTGGACAGAGTGT
TCCACAAGCTGCGGGGAAGGCACCCAGACTCGAAGCGCCATTTGCCGAAAGATGCTGAAA
ACCGGCCTCTCAACGGTTGTCAATTCCACCCTGTGCCCGCCCCTGCCTTTCTCTTCCTCC
ATCAGGCCCTGTATGCTGGCAACCTGTGCAAGGCCCGGGCGGCCATCCACGAAGCACAGC
CCGCACATCGCGGCCGCCAGGAAGGTCTACATACAGACTCGCAGGCAGAGGAAGCTGCAC
TTCGTGGTGGGGGGCTTCGCCTACCTGCTCCCCAAGACGGCGGTGGTGCTGCGCTGCCCG
GCGCGCAGGGTCCGCAAGCCCCTCATCACCTGGGAGAAGGACGGCCAGCACCTCATCAGC
TCGACGCACGTCACGGTGGCCCCCTTCGGCTATCTCAAGATCCACCGCCTCAAGCCCTCG
GATGCAGGCGTCTACACCTGCTCAGCGGGCCCGGCCCGGGAGCACTTTGTGATTAAGCTC
ATCGGAGGCAACCGCAAGCTCGTGGCCCGGCCCTTGAGCCCGAGAAGTGAGGAAGAGGTG
CTTGCGGGGAGGAAGGGCGGCCCGAAGGAGGCCCTGCAGACCCACAAACACCAGAACGGG
ATCTTCTCCAACGGCAGCAAGGCGGAGAAGCGGGGCCTGGCCGCCAACCCGGGGAGCCGC
TACGACGACCTCGTCTCCCGGCTGCTGGAGCAGGGCGGCTGGCCCGGAGAGCTGCTGGCC
TCGTGGGAGGCGCAGGACTCTGCGGAAAGGAACACGACCTCGGAGGAGGACCCGGGTGCA
GAGCAAGTGCTCCTGCACCTGCCCTTCACCATGGTGACCGAGCAGCGGCGCCTGGACGAC
ATCCTGGGGAACCTCTCCCAGCAGCCCGAGGAGCTGCGCGACCTCTACAGCAAGCACCTG
GTGGCCCAGCTGGCCCAGGAGATCTTCCGCAGCCACCTGGAGCACCAGGACACGCTCCTG
AAGCCCTCGGAGCGCAGGACTTCCCCAGTGACTCTCTCGCCTCATAAACACGTGTCTGGC
TTCAGCAGCTCCCTGCGGACCTCCTCCACCGGGGACGCCGGGGGAGGCTCTCGAAGGCCA
CACCGCAAGCCCACCATCCTGCGCAAGATCTCAGCGGCCCAGCAGCTCTCAGCCTCGGAG
GTGGTCACCCACCTGGGGCAGACGGTGGCCCTGGCCAGCGGGACACTGAGTGTTCTTCTG
CACTGTGAGGCCATCGGCCACCCAAGGCCTACCATCAGCTGGGCCAGGAATGGAGAAGAA
GTTCAGTTCAGTGACAGGATTCTTCTACAGCCAGATGATTCCTTACAGATCTTGGCACCA
GTGGAAGCAGATGTGGGTTTCTACACTTGCAATGCCACCAATGCCTTGGGATACGACTCT
GTCTCCATTGCCGTCACATTAGCAGGAAAGCCACTAGTGAAAACGTCACGAATGACAGTG
ATCAACACGGAGAAGCCTGCAGTCACAGTCGATATAGGAAGCACCATCAAAACAGTGCAG
GGAGTGAATGTGACAATCAACTGCCAGGTTGCAGGAGTGCCTGAAGCTGAAGTCACTTGG
TTCAGGAATAAAAGCAAACTGGGCTCCCCGCACCATCTGCACGAAGGCTCCTTGCTGCTC
ACAAACGTGTCCTCCTCGGATCAGGGCCTGTACTCCTGCAGGGCGGCCAATCTTCATGGA
GAGCTGACTGAGAGCACCCAGCTGCTGATCCTAGATCCCCCCCAAGTCCCCACACAGTTG
GAAGACATCAGGGCCTTGCTCGCTGCCACTGGACCGAACCTTCCTTCAGTGCTGACGTCT
CCTCTGGGAACACAGCTGGTCCTGGATCCTGGGAATTCTGCTCTCCTTGGCTGCCCCATC
AAAGGTCACCCTGTCCCTAATATCACCTGGTTTCATGGTGGTCAGCCAATTGTCACTGCC
ACAGGACTGACGCATCACATCTTGGCAGCTGGACAGATCCTTCAAGTTGCAAACCTTAGC
GGTGGGTCTCAAGGGGAATTCAGCTGCCTTGCTCAGAATGAGGCAGGGGTGCTCATGCAG
AAGGCATCTTTAGTGATCCAAGATTACTGGTGGTCTGTGGACAGACTGGCAACCTGCTCA
GCCTCCTGTGGTAACCGGGGGGTTCAGCAGCCCCGCTTGAGGTGCCTGCTGAACAGCACG
GAGGTCAACCCTGCCCACTGCGCAGGGAAGGTTCGCCCTGCGGTGCAGCCCATCGCGTGC
AACCGGAGAGACTGCCCTTCTCGGTGGATGGTGACCTCCTGGTCTGCCTGTACCCGGAGC
TGTGGGGGAGGTGTCCAGACCCGCAGGGTGACCTGTCAAAAGCTGAAAGCCTCTGGGATC
TCCACCCCTGTGTCCAATGACATGTGCACCCAGGTCGCCAAGCGGCCTGTGGACACCCAG
GCCTGTAACCAGCAGCTGTGTGTGGAGTGGGCCTTCTCCAGCTGGGGCCAGTGCAATGGG
CCTTGCATCGGGCCTCACCTAGCTGTGCAACACAGACAAGTCTTCTGCCAGACACGGGAT
GGCATCACCTTACCATCAGAGCAGTGCAGTGCTCTTCCGAGGCCTGTGAGCACCCAGAAC
TGCTGGTCAGAGGCCTGCAGTGTACACTGGAGAGTCAGCCTGTGGACCCTGTGCACAGCT
ACCTGTGGCAACTACGGCTTCCAGTCCCGGCGTGTGGAGTGTGTGCATGCCCGCACCAAC
AAGGCAGTGCCTGAGCACCTGTGCTCCTGGGGGCCCCGGCCTGCCAACTGGCAGCGCTGC
AACATCACCCCATGTGAAAACATGGAGTGCAGAGACACCACCAGGTACTGCGAGAAGGTG
AAACAGCTGAAACTCTGCCAACTCAGCCAGTTTAAATCTCGCTGCTGTGGAACTTGTGGC
AAAGCGTTG
Protein sequence predicted based on nucleotide sequence
MECCRRATPGTLLLFLAFLLLSSRTARSEEDRDGLWDAWGPWSECSRTCGGGASYSLRRC LSSKSCEGRNIRYRTCSNVDCPPEAGDFRAQQCSAHNDVKHHGQFYEWLPVSNDPDNPCS LKCQAKGTTLVVELAPKVLDGTRCYTESLDMCISGLCQIVGCDHQLGSTVKEDNCGVCNG DGSTCRLVRGQYKSQLSATKSDDTVVAIPYGSRHIRLVLKGPDHLYLETKTLQGTKGENS LSSTGTFLVDNSSVDFQKFPDKEILRMAGPLTADFIVKIRNSGSADSTVQFIFYQPIIHR WRETDFFPCSATCGGGYQLTSAECYDLRSNRVVADQYCHYYPENIKPKPKLQECNLDPCP ASDGYKQIMPYDLYHPLPRWEATPWTACSSSCGGGIQSRAVSCVEEDIQGHVTSVEEWKC MYTPKMPIAQPCNIFDCPKWLAQEWSPCTVTCGQGLRYRVVLCIDHRGMHTGGCSPKTKP HIKEECIVPTPCYKPKEKLPVEAKLPWFKQAQELEEGAAVSEEPSFIPEAWSACTVTCGV GTQVRIVRCQVLLSFSQSVADLPIDECEGPKPASQRACYAGPCSGEIPEFNPDETDGLFG GLQDFDELYDWEYEGFTKCSESCGGGVQEAVVSCLNKQTREPAEENLCVTSRRPPQLLKS CNLDPCPARWEIGKWSPCSLTCGVGLQTRDVFCSHLLSREMNETVILADELCRQPKPSTV QACNRFNCPPAWYPAQWQPCSRTCGGGVQKREVLCKQRMADGSFLELPETFCSASKPACQ QACKKDDCPSEWLLSDWTECSTSCGEGTQTRSAICRKMLKTGLSTVVNSTLCPPLPFSSS IRPCMLATCARPGRPSTKHSPHIAAARKVYIQTRRQRKLHFVVGGFAYLLPKTAVVLRCP ARRVRKPLITWEKDGQHLISSTHVTVAPFGYLKIHRLKPSDAGVYTCSAGPAREHFVIKL IGGNRKLVARPLSPRSEEEVLAGRKGGPKEALQTHKHQNGIFSNGSKAEKRGLAANPGSR YDDLVSRLLEQGGWPGELLASWEAQDSAERNTTSEEDPGAEQVLLHLPFTMVTEQRRLDD ILGNLSQQPEELRDLYSKHLVAQLAQEIFRSHLEHQDTLLKPSERRTSPVTLSPHKHVSG FSSSLRTSSTGDAGGGSRRPHRKPTILRKISAAQQLSASEVVTHLGQTVALASGTLSVLL HCEAIGHPRPTISWARNGEEVQFSDRILLQPDDSLQILAPVEADVGFYTCNATNALGYDS VSIAVTLAGKPLVKTSRMTVINTEKPAVTVDIGSTIKTVQGVNVTINCQVAGVPEAEVTW FRNKSKLGSPHHLHEGSLLLTNVSSSDQGLYSCRAANLHGELTESTQLLILDPPQVPTQL EDIRALLAATGPNLPSVLTSPLGTQLVLDPGNSALLGCPIKGHPVPNITWFHGGQPIVTA TGLTHHILAAGQILQVANLSGGSQGEFSCLAQNEAGVLMQKASLVIQDYWWSVDRLATCS ASCGNRGVQQPRLRCLLNSTEVNPAHCAGKVRPAVQPIACNRRDCPSRWMVTSWSACTRS CGGGVQTRRVTCQKLKASGISTPVSNDMCTQVAKRPVDTQACNQQLCVEWAFSSWGQCNG PCIGPHLAVQHRQVFCQTRDGITLPSEQCSALPRPVSTQNCWSEACSVHWRVSLWTLCTA TCGNYGFQSRRVECVHARTNKAVPEHLCSWGPRPANWQRCNITPCENMECRDTTRYCEKV KQLKLCQLSQFKSRCCGTCGKAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
