Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868069
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6486nts
Open reading frame: 1 to 6486
ATGACCCACAGCCCCGCGACAAGCGAGGACGAGGAACGCCACAGTGCCAGCGAGTGTCCC
GAGGGGGGCTCAGAGTCCGACAGCTCCCCAGACGGGCCAGGTCGAGGCCCCCGGGGGACC
CGGGGCCAGGGCAGTGGGGCACCTGGTAGCCTGGCCTCTGTTAGAGGCCTCCAGGGCCGC
TCAATGTCCGTCCCAGACGACGCCCACTTCAGCATGATGGTCTTCAGGATTGGCATCCCG
GACCTGCACCAGACAAAATGCCTTCGCTTCAACCCCGATGCCACCATCTGGACGGCCAAG
CAGCAGGTGCTCTGTGCCCTGAGCGAGAGCCTGCAGGATGTGCTCAACTATGGCCTGTTC
CAACCGGCCACCTCCGGCCGCGATGCCAACTTCCTGGAGGAGGAGAGGCTGCTGCGGGAG
TACCCCCAGTCCTTTGAGAAGGGGGTCCCCTACCTGGAGTTCCGATACAAGACCCGAGTT
TACAAACAGACCAACCTGGATGAGAAGCAGCTGGCCAAGTTGCACACGAAGACGGGGTTG
AAGAAGTTCCTGGAGTATGTGCAGCTCGGGACATCTGACAAGGTGGCGCGGCTGCTGGAC
AAGGGGCTGGACCCCAATTACCATGACTCGGATTCGGGAGAGACCCCCTTGACACTGGCG
GCCCAGACCGAAGGCTCTGTAGAGGTGATTCGAACCCTGTGCCTGGGCGGGGCCCACATT
GACTTCCGGGCCCGGGATGGCATGACCGCACTGCATAAGGCCGCATGCGCCCGACACTGC
CTGGCACTCACGGCGCTCCTGGACCTTGGGGGTTCCCCCAACTACAAGGACCGTCGGGGG
CTGACCCCTCTGTTCCACACGGCCATGGTGGGTGGTGACCCCCGATGCTGCGAGCTGCTC
CTGTTCAACAGGGCCCAGCTGGGCATAGCTGATGAGAACGGCTGGCAGGAAATCCACCAG
GCCTGCCAGCGGGGTCACTCTCAGCACCTGGAGCATCTGCTTTTCTACGGGGCTGAGCCT
GGAGCCCAGAACGCCTCGGGGAACACGGCTCTGCACATCTGCGCCCTCTACAACAAGGAG
ACCTGTGCCAGGATCCTCCTGTATCGAGGTGCCGACAAGGATGTGAAGAACAACAACGGA
CAGACCCCCTTCCAGGTGGCAGTGATTGCTGGGAATTTTGAGCTGGGGGAGCTGATCCGA
AACCACCGAGAACAGGATGTGGTGCCCTTCCAGGAGTCCCCCAAGTACGCGGCCCGGCGA
CGGGGGCCCCCAGGCACAGGGCTGACGGTGCCCCCGGCGCTGCTGCGGGCCAACAGTGAC
ACCAGCATGGCGCTGCCCGACTGGATGGTGTTCTCCGCCCCGGGGGCCGCGTCCTCTGGG
GCCCCTGGCCCTACCTCAGGGTCCCAGGGCCAGTCGCAGCCCTCGGCCCCCACCACCAAG
CTCAGCAGCGGGACCCTCCGAAGTGCCAGCAGCCCCCGGGGTGCCAGGGCCCGCTCTCCA
TCCCGAGGGAGGCACCCTGAGGACGCCAAGAGGCAGCCCCGAGGCCGGCCCAGCTCCAGC
GGGACACCCCGGGAAGGGCCAGCCGGGGGCACGGGGGGCTCAGGGGGCCCCGGGGGCTCC
CTGGGCAGCCGCGGGAGGCGGAGGAAGCTCTACTCAGCGGTACCCGGACGCTCCTTCATG
GCTGTGAAGTCCTACCAGGCCCAAGCCGAGGGGGAGATCTCCCTGAGCAAGGGCGAGAAG
ATCAAAGTACTTAGCATCGGGGAAGGAGGCTTCTGGGAAGGCCAGGTCAAAGGTCGTGTT
GGCTGGTTCCCCTCTGACTGCCTGGAAGAAGTGGCGAATCGCTCTCAGGAGAGCAAGCAA
GAAAGCCGCAGTGACAAGGCAAAGAGACTCTTCCGGCATTATACCGTGGGCTCCTACGAC
AGCTTTGATGCCCCAAGCTTAATGGATGGGATTGGCCCAGGGAGCGATTACATCATTAAG
GAGAAGACAGTCTTGCTGCAGAAGAAGGACAGTGAGGGGTTTGGGTTCGTGCTCCGGGGG
GCCAAGGCGCAGACCCCCATCGAGGAGTTCACCCCCACCCCGGCCTTCCCGGCGCTGCAG
TACCTGGAGTCGGTGGACGAGGGTGGCGTGGCATGGCGAGCTGGACTGCGAATGGGAGAC
TTCCTCATCGAGGTGAACGGGCAGAATGTGGTGAAGGTCGGCCACCGACAGGTGGTGAAC
ATGATCCGCCAAGGGGGCAACACGCTGATGGTGAAGGTGGTGATGGTCACCAGGCACCCG
GACATGGATGAGGCAGTGCACAAGAAAGCACCCCAGCAGGCCAAGCGGCTGCCGCCCCCA
ACCATCTCCCTGCGTTCCAAATCTATGACCTCAGAGCTGGAGGAGATGGAGTACGAGCAG
CAGCCGGCGCCGGTGCCCAGCATGGAGAAAAAGCGGACCGTGTATCAGATGGCTCTCAAC
AAACTGGACGAAATCCTGGCCGCAGCTCAACAGACCATCAGTGCAAGCGAAAGCCCTGGT
CCCGGTGGCCTCGCGTCCCTGGGCAAACACCGACCCAAAGGTTTCTTTGCCACTGAGTCG
AGCTTCGATCCCCACCACCGTGCCCAGCCAAGTTACGAGCGTCCTTCTTTCCTGCCTCCA
GGACCTGGGTTGATGCTCCGGCAAAAATCTATCGGTGCGGCAGAAGATGACAGACCTTAC
CTAGCACCCCCAGCCATGAAATTCAGCCGCAGCCTGTCTGTGCCTGGTTCGGAGGACATT
CCCCCGCCACCCACCACGTCCCCACCGGAGCCTCCCTACAGCACACCTCCAGTCCCCTCC
TCCTCAGGGCGCCTCACCCCCTCCCCTCGGGGAGGGCCCTTCAACCCTGGCTCTGGTGGC
CCCCTCCCCGCCTCCTCCCCTGCATCCTTTGACGGGCCCTCCCCTCCCGACACTCGCGTG
GGGAGCCGCGAGAAGAGCCTGTACCACAGTGGGCCCCTGCCCCCGGCCCACCACCACCCG
CCCCACCACCACCACCACCACGCCCCGCCCCCTCAGCCCCACCACCACCACGCCCACCCC
CCTCATCCTCCCGAGATGGAGACAGGCGGCTCTCCCGACGACCCTCCACCCCGCCTGGCT
CTGGGGCCCCAGCCCAGCCTGCGAGGCTGGAGGGGCGGCGGGCCCAGCCCGACCCCGGGG
GCCCCGTCCCCATCGCACCACGGCAGCGCGGGCGGGGGCGGCGGCTCCTCCCAGGGCCCG
GCTCTACGCTATTTCCAGCTGCCCCCGCGGGCGGCCAGCGCAGCCATGTACGTGCCCGCC
CGCTCGGGCCGCGGCCGCAAGGGCCCGCTGGTCAAGCAGACCAAGGTGGAAGGCGAGCCC
CAGAAGGGCGGCGGCCTCCCGCCCGCGCCGTCGCCCACGTCCCCGGCCTCCCCGCAGCCG
CCGCCCGCCGTGGCCGCGCCCTCGGAGAAGAACTCCATCCCCATCCCCACCATCATCATC
AAGGCCCCGTCCACCAGTAGCAGCGGCCGCAGCAGCCAGGGCAGCAGCACCGAGGCGGAG
CCCCCCACCCAGCCGGAGCCCACGGGAGGCGGCGGCGGCGGCGGCTCCTCGCCCAGCCCC
GCCCCGGCCATGTCACCCGTGCCCCCGTCCCCCTCGCCCGTGCCCACCCCCGCCTCGCCC
AGCGGCCCGGCCACGCTGGACTTCACGAGCCAGTTCGGGGCCGCCCTGGTGGGGGCGGCC
CGGAGGGAGGGGGGCTGGCAGAATGAGGCGCGCCGGCGCTCCACGCTGTTCCTGTCCACC
GACGCGGGGGACGAGGACGGCGGGGACGGCGGGCTGGGCACAGGGGCGGCCCCGGGCCCG
CGGCTGCGCCACTCCAAATCCATCGACGAGGGCATGTTCTCCGCCGAGCCCTACCTCCGA
CTGGAGTCTGCGGGCAGCGGCGCGGGCTACGGCGGCTACGGGGCCGGTAGCCGAGCCTAC
GGGGGTGGCGGGGGCAGCAGCGCCTTCACCAGCTTCCTGCCCCCGCGACCCCTGGTGCAC
CCGCTGACCGGCAAGGCCCTGGATCCCGCCTCCCCGCTGGGGCTGGCCCTGGCCGCCCGC
GAGCGAGCGCTGAAGGAGTCCTCGGAGGGCGGCGGGGCCCCCCAGCCGCCTCCCAGGCCC
CCATCGCCCCGCTACGAGGCCCCGCCGCCCACCCCGCACCACCACTCGCCCCACGCCCAC
CACGAGCCAGTGCTGCGTCTCTGGGGGGCCTCCCCGCCGGACCCTGCGCGCCGGGAGCTG
GGGTACAGGGCCGGGCTGGGCAGCCAGGAGAAGTCCCTTCCCGCCAGCCCGCCCGCCGCC
CGGCGTTCCCTGCTACACCGCCTGCCGCCCACCGCTCCCGGGGTGGGGCCCCTCCTGCTG
CAGCTGGGGACGGAGCCCCCGGCCCCGCACCCCGGAGTAAGCAAGCCCTGGAGGTCCGCA
GCCCCCGAAGAACCCGAGCGGCTGCCGCTGCACGTGCGGTTCCTTGAAAACTGCCAGCCC
CGGGCCCCTGTGACGAGCGGAAGGGGTCCCCCCTCGGAGGACGGGCCGGGGGTCCCGCCG
CCCAGCCCACGCCGGTCCGTGCCCCCCTCCCCGACCTCCCCGAGGGCCAGCGAAGAGAAC
GGGCTGCCCCTGCTGGTCCTGCCGCCTCCCGCCCCCTCGGTGGATGTGGAAGATGGCGAA
TTCCTTTTCGTGGAACCGCTGCCTCCGCCTCTGGAATTCTCCAACAGCTTCGAAAAGCCA
GAGTCGCCCCTCACGCCTGGGCCTCCCCACCCGCTGCCCGACACACCTGCCCCTGCCACC
CCGTTACCCCCTGTGCCACCCCCGGCTGTGGCCGCAGCCCCTCCCACCCTGGACTCCACC
GCATCCAGCCTGACATCCTATGACAGCGAGGTGGCCACCCTGACCCAGGGGGCCTCCGCC
GCTCCTGGGGACCCCCATCCACCAGGCCCGCCTGCCCCAGCAGCACCGGCTCCCGCTGCC
CCACAGCCTGGCCCGGACCCTCCGCCTGGCACGGATTCTGGCATCGAGGAGGTGGACAGT
CGGAGCAGCAGTGACCACCCACTGGAGACCATCAGCAGCGCCTCCACGCTGAGCAGCCTA
TCTGCCGAAGGTGGTGGCAGCGCAGGGGGTGGGGGCGGGGCTGGGGCCGGTGTGGCCAGT
GGGCCGGAGCTTCTGGACACCTATGTGGCCTACCTGGACGGCCAGGCCTTTGGGGGCAGC
AGTACTCCCGGCCCGCCATACCCTCCTCAGCTCATGACTCCCTCTAAGCTCCGGGGCCGG
GCGCTAGGAGCCAGCGGAGGCCTGCGGCCTGGCCCCAGCGGGGGACTCCGAGACCCTGTT
ACCCCCACCAGCCCCACCGTCTCGGTGACAGGGGCTGGAACCGATGGGCTGCTGGCCCTG
CGTGCTTGTTCAGGACCCCCCACGGCAGGCGTGGCGGGGGGTCCGGTGGCTGTAGAGCCA
GAAGTCCCACCGGTGCCCTTGCCGACGGCCTCCTCTCTGCCCCGGAAGCTGCTGCCCTGG
GAGGAGGGCCCGGGCCCACCGCCACCACCTCTGCCCGGGCCCTTGGCCCAGCCTCAGGCC
TCAGCCTTGGCCACAGTAAAAGCCAGCATCATCAGTGAACTCAGCTCCAAGCTTCAGCAG
TTTGGGGGCTCCTCGGCAGCTGGCGGCGCTCTGCCCTGGGCCCGAGGAGGCAGTGGGGGA
GGCGGAGACAGCCACCACGGGGGAGCCAGCTATGTCCCCGAGAGGACCTCCTCCCTGCAG
CGGCAGAGACTCTCCGACGACTCCCAGTCCTCACTCCTCTCCAAGCCTGTCAGCAGCCTG
TTTCAGAACTGGCCCAAACCACCTCTGCCGCCACTCCCCACCGGAACAGGGGTCTCCCCT
ACAGCCGCTGCGGCCCCAGGGGCCACCTCACCCTCAGCCTCCTCCTCCTCCACGTCCACC
CGCCACCTCCAGGGCGTGGAGTTCGAGATGCGGCCCCCTCTGCTCCGCCGGGCCCCCAGC
CCCTCGCTGCTGCCCGCCTCGGAGCACAAGGTCAGCCCTGCGCCCAGGCCCTCGTCCCTG
CCCATCCTGCCTTCCGGACCCCTCTACCCAGGCCTCTTTGACATCCGTGGCTCCCCAACT
GGAGGGGCAGGAGGCTCGGCTGACCCCTTCGCCCCAGTCTTTGTGCCGCCACACCCGGGG
ATATCCGGGGGGCTCGGGGGAGCCTTGTCAGGGGCCTCGCGCTCCCTCTCACCGACCCGC
CTGCTCTCGCTGCCCCCGGACAAGCCGTTTGGCGCTAAACCTCTGGGGTTCTGGACCAAG
TTCGACGTGGCTGATTGGCTGGAGTGGCTGGGTTTGGCGGAGCACCGAGCCCAGTTCCTG
GACCACGAGATCGATGGCTCCCACCTGCCCGCCTTGACCAAGGAGGACTACGTCGATCTA
GGTGTGACCAGGGTGGGCCACCGCATGAACATCGACCGGGCTCTCAAATTCTTCCTGGAG
AGGTTG
Protein sequence predicted based on nucleotide sequence
MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPRGTRGQGSGAPGSLASVRGLQGR SMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLF QPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGL KKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHI DFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELL LFNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKE TCARILLYRGADKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARR RGPPGTGLTVPPALLRANSDTSMALPDWMVFSAPGAASSGAPGPTSGSQGQSQPSAPTTK LSSGTLRSASSPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPREGPAGGTGGSGGPGGS LGSRGRRRKLYSAVPGRSFMAVKSYQAQAEGEISLSKGEKIKVLSIGEGGFWEGQVKGRV GWFPSDCLEEVANRSQESKQESRSDKAKRLFRHYTVGSYDSFDAPSLMDGIGPGSDYIIK EKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGD FLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKAPQQAKRLPPP TISLRSKSMTSELEEMEYEQQPAPVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPG PGGLASLGKHRPKGFFATESSFDPHHRAQPSYERPSFLPPGPGLMLRQKSIGAAEDDRPY LAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPVPSSSGRLTPSPRGGPFNPGSGG PLPASSPASFDGPSPPDTRVGSREKSLYHSGPLPPAHHHPPHHHHHHAPPPQPHHHHAHP PHPPEMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTPGAPSPSHHGSAGGGGGSSQGP ALRYFQLPPRAASAAMYVPARSGRGRKGPLVKQTKVEGEPQKGGGLPPAPSPTSPASPQP PPAVAAPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPEPTGGGGGGGSSPSP APAMSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLST DAGDEDGGDGGLGTGAAPGPRLRHSKSIDEGMFSAEPYLRLESAGSGAGYGGYGAGSRAY GGGGGSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGAPQPPPRP PSPRYEAPPPTPHHHSPHAHHEPVLRLWGASPPDPARRELGYRAGLGSQEKSLPASPPAA RRSLLHRLPPTAPGVGPLLLQLGTEPPAPHPGVSKPWRSAAPEEPERLPLHVRFLENCQP RAPVTSGRGPPSEDGPGVPPPSPRRSVPPSPTSPRASEENGLPLLVLPPPAPSVDVEDGE FLFVEPLPPPLEFSNSFEKPESPLTPGPPHPLPDTPAPATPLPPVPPPAVAAAPPTLDST ASSLTSYDSEVATLTQGASAAPGDPHPPGPPAPAAPAPAAPQPGPDPPPGTDSGIEEVDS RSSSDHPLETISSASTLSSLSAEGGGSAGGGGGAGAGVASGPELLDTYVAYLDGQAFGGS STPGPPYPPQLMTPSKLRGRALGASGGLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLAL RACSGPPTAGVAGGPVAVEPEVPPVPLPTASSLPRKLLPWEEGPGPPPPPLPGPLAQPQA SALATVKASIISELSSKLQQFGGSSAAGGALPWARGGSGGGGDSHHGGASYVPERTSSLQ RQRLSDDSQSSLLSKPVSSLFQNWPKPPLPPLPTGTGVSPTAAAAPGATSPSASSSSTST RHLQGVEFEMRPPLLRRAPSPSLLPASEHKVSPAPRPSSLPILPSGPLYPGLFDIRGSPT GGAGGSADPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTK FDVADWLEWLGLAEHRAQFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLE RL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
