Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868185
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3057nts
Open reading frame: 1 to 3057
ATGGTTTCCCATTTCATGGGGTCTCTCAGTGTCCTGTGTTTCCTTCTGCTGCTTGGATTC
CAGTTCGTCTGCCCACAGCCCTCCACTCAACACAGGAAGGTCCCGCAGCGGATGGCGGCG
GAGGGCGCCCCCGAGGACGACGGCGGCGGCGGCGCCCCGGGAGTGTGGGGCGCCTGGGGC
CCCTGGTCGGCCTGCTCGCGTAGCTGCAGCGGCGGCGTGATGGAGCAGACGCGGCCCTGC
CTGCCCCGCTCCTACCGCCTGCGCGGCGGCCAGCGGCCTGGCGCCCCTGCGCGCGCCTTC
GCGGACCACGTGGTGTCGGCGGTGCGCACGTCGGTGCCACTGCACCGGAGCCGCGACGAG
ACGCCAGCGCTGGCCGGTACGGACGCCAGCCGCCAGGGCCCCACGGTGCTGCGAGGCAGC
CGGCACCCACAGCCCCAGGGCCTCGAAGTCACTGGGGACAGAAGGAGCAGGACCCGTGGT
ACCATTGGCCCTGGCAAGTATGGCTATGGTAAGGCCCCATATATCTTACCACTGCAGACA
GACACTGCACACACGCCACAGAGGCTCCGGAGACAGAAGCTCTCATCCCGCCATTCCAGG
TCCCAGGGAGCATCTTCTGCTAGGCATGGCTACAGTTCACCAGCCCACCAGGTCCCCCAA
CATGGGCCTTTGTACCAAAGTGACAGTGGCCCTCGCTCTGGACTGCAGGCTGCGGAGGCC
CCCATCTACCAGCTACCTTTGACCCATGATCAAGGCTACCCTGCAGCTTCAAGTCTCTTT
CACAGCCCAGAAACAAGCAACAACCACGGTGTGGGGACCCATGGGGCAACTCAGAGCTTC
TCTCAGCCTGCCCGATCTACAGCAATCTCATGCATCGGGGCCTATCGGCAGTACAAGCTG
TGCAACACCAACGTATGTCCAGAAAGCAGTAGAAGTATCCGGGAGGTACAGTGTGCATCC
TACAACAACAAGCCATTCATGGGCCGGTTTTATGAGTGGGAACCATTTGCAGAAGTAAAA
GGCAATCGCAAATGTGAGTTGAACTGCCAGGCAATGGGCTACCGCTTCTATGTACGGCAA
GCTGAGAAAGTCATCGATGGCACCCCCTGTGACCAGAACGGCACGGCCATCTGTGTGTCT
GGGCAGTGCAAGAGCATTGGCTGTGATGACTACTTAGGCTCCGACAAAGTCGTGGACAAA
TGTGGGGTGTGTGGAGGAGACAACACGGGCTGTCAGGTTGTGTCGGGCGTGTTTAAGCAT
GCCCTCACCAGCCTGGGCTACCACCGCGTCGTGGAGATTCCCGAGGGAGCCACGAAAATC
AACATCACGGAGATGTACAAGAGCAACAACTATTTGGCCCTGAGAAGTCGTTCTGGACGC
TCCATCATCAATGGGAACTGGGCAATTGATCGACCAGGAAAATACGAGGGCGGAGGGACC
ATGTTCACCTACAAGCGTCCAAATGAGATTTCGAGCACTGCCGGAGAGTCCTTTTTGGCG
GAAGGTCCCACCAACGAGATCTTGGATGTCTACATGATACACCAGCAGCCAAACCCAGGC
GTGCACTACGAGTACGTGATCATGGGGACCAACGCCATCAGCCCCCAGGTGCCACCCCAC
AGGAGACCAGGGGAACCCTTCAATGGCCAGATGGTGACAGAAGGCAGGAGCCAGGAGGAG
GGAGAACAGAAAGGGAGGAACGAGGAGAAGGAAGACTTGCGTGGGGAGGCCCCTGAGATG
TTCACCTCAGAATCGGCACAGACCTTCCCAGTCAGGCATCCAGACAGATTTTCTCCCCAT
CGACCGGACAACTTGGTGCCACCAGCACCGCAGCCCCCACGGCGCAGCCGGGATCACAAC
TGGAAGCAGCTTGGGACAACAGAATGTTCCACGACCTGTGGGAAAGGATCGCAGTACCCT
ATTTTCCGCTGTGTGCACAGAAGCACTCATGAAGAGGCTCCTGAGAGTTACTGTGACTCC
AGCATGAAGCCGACCCCCGAGGAGGAGCCCTGCAACATCTTCCCTTGCCCAGCCTTCTGG
GACATCGGGGAGTGGTCTGAGTGCAGCAAGACCTGTGGCCTGGGCATGCAGCACCGCCAG
GTTCTGTGCCGCCAGGTGTACGCCAACCGCAGCCTGACGGTGCAGCCCTACCGCTGCCAG
CACCTGGAGAAACCTGAGACCACCAGCACCTGCCAACTCAAGATCTGCAGCGAGTGGCAG
ATCCGGACCGACTGGACCTCGTGCTCGGTGCCCTGCGGCGTGGGACAGAGGACCCGTGAT
GTGAAGTGTGTGAGCAACATTGGGGATGTGGTTGACGATGAGGAATGCAACATGAAGCTC
CGGCCGAATGACATTGAGAACTGCGACATGGGACCCTGTGCCAAGAGCTGGTTCCTCACC
GAGTGGAGCGAAAGGTGCTCAGCGGAGTGTGGGGCCGGAGTGCGGACACGCTCGGTGGTG
TGCATGACCAACCATGTCAGCAGCCTGCCCCTGGAGGGCTGTGGGAACAACCGGCCGGCA
GAGGCCACCCCATGTGACAACGGACCCTGCACGGGCAAGGTGGAGTGGTTTGCCGGGAGC
TGGAGTCAGTGTTCCATCGAGTGTGGGAGCGGGACGCAACAGAGGGAGGTGATTTGTGTT
AGAAAGAATGCAGACACCTTTGAAGTGTTGGACCCCTCTGAATGTTCTTTCCTGGAGAAA
CCCCCCAGCCAGCAATCCTGCCACCTCAAGCCTTGCGGAGCCAAATGGTTTAGCACCGAA
TGGAGCATGTGTTCCAAGAGCTGCCAGGGTGGCTTTCGGGTCCGGGAAGTGCGGTGTCTG
TCTGATGACATGACTCTAAGTAACCTCTGTGACCCTCAGTTGAAACCAGAAGAGAGAGAA
TCTTGTAACCCTCAGGACTGTGTCCCTGAAGTTGATGAAAACTGCAAGGACAAGTACTAC
AACTGCAACGTGGTGGTCCAGGCAAGACTCTGTGTCTACAACTACTACAAGACCGCCTGC
TGTGCCTCCTGCACCCGTGTGGCCAACAGGCAGACGGGCTTCCTGGGGAGCAGATTG
Protein sequence predicted based on nucleotide sequence
MVSHFMGSLSVLCFLLLLGFQFVCPQPSTQHRKVPQRMAAEGAPEDDGGGGAPGVWGAWG PWSACSRSCSGGVMEQTRPCLPRSYRLRGGQRPGAPARAFADHVVSAVRTSVPLHRSRDE TPALAGTDASRQGPTVLRGSRHPQPQGLEVTGDRRSRTRGTIGPGKYGYGKAPYILPLQT DTAHTPQRLRRQKLSSRHSRSQGASSARHGYSSPAHQVPQHGPLYQSDSGPRSGLQAAEA PIYQLPLTHDQGYPAASSLFHSPETSNNHGVGTHGATQSFSQPARSTAISCIGAYRQYKL CNTNVCPESSRSIREVQCASYNNKPFMGRFYEWEPFAEVKGNRKCELNCQAMGYRFYVRQ AEKVIDGTPCDQNGTAICVSGQCKSIGCDDYLGSDKVVDKCGVCGGDNTGCQVVSGVFKH ALTSLGYHRVVEIPEGATKINITEMYKSNNYLALRSRSGRSIINGNWAIDRPGKYEGGGT MFTYKRPNEISSTAGESFLAEGPTNEILDVYMIHQQPNPGVHYEYVIMGTNAISPQVPPH RRPGEPFNGQMVTEGRSQEEGEQKGRNEEKEDLRGEAPEMFTSESAQTFPVRHPDRFSPH RPDNLVPPAPQPPRRSRDHNWKQLGTTECSTTCGKGSQYPIFRCVHRSTHEEAPESYCDS SMKPTPEEEPCNIFPCPAFWDIGEWSECSKTCGLGMQHRQVLCRQVYANRSLTVQPYRCQ HLEKPETTSTCQLKICSEWQIRTDWTSCSVPCGVGQRTRDVKCVSNIGDVVDDEECNMKL RPNDIENCDMGPCAKSWFLTEWSERCSAECGAGVRTRSVVCMTNHVSSLPLEGCGNNRPA EATPCDNGPCTGKVEWFAGSWSQCSIECGSGTQQREVICVRKNADTFEVLDPSECSFLEK PPSQQSCHLKPCGAKWFSTEWSMCSKSCQGGFRVREVRCLSDDMTLSNLCDPQLKPEERE SCNPQDCVPEVDENCKDKYYNCNVVVQARLCVYNYYKTACCASCTRVANRQTGFLGSRL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
