Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868225
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4812nts
Open reading frame: 1 to 4812
ATGAGAACAACAAAGGTCTACAAACTCGTCATCCACAAGAAGGGCTTTGGGGGCAGTGAT
GATGAGCTAGTTGTGAACCCCAAAGTGTTCCCTCACATCAAGCTTGGAGACATTGTAGAG
ATTGCACACCCCAACGATGAATACAGCCCTCTGCTTTTGCAGGTCAAGTCTCTTAAGGAA
GATTTACAGAAGGAAACTATCAGTGTGGACCAGACTGTGACTCAAGTGTTCCGGCTGAGA
CCTTATCAGGATGTCTATGTTAATGTCGTAGACCCTAAGGATGTGACCCTTGACCTAGTG
GAATTAACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGGCGACTAAAGAAAAGT
TTGGTCAGCACATGTGCCTATATCACCCAGAAGGTGGAGTTTGCTGGCATCAGAGCACAG
GCTGGTGAACTGTGGGTTAAGAATGAGAAGGTCATGTGTGGCTACATCAGTGAAGATACC
AGGGTGGTGTTTCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAA
ATGTGGGATTTTGATATTTATGGGGATTTGTATTTTGAGAAAGCTGTGAATGGTTTCCTT
GCTGATCTATTTACCAAGTGGAAGGAGAAGAACTGTAGTCATGAAGTGACAGTGGTCCTG
TTTTCTAGAACTTTCTATGATGCAAAATCTGTTGATGAATTTCCTGAAATAAACCGAGCC
TCAATTCGACAGGATCACAAGGGGAGATTCTATGAAGACTTTTACAAAGTGGTGGTGCAG
AATGAGAGAAGAGAAGAATGGACTTCACTTCTCGTAACCATTAAAAAACTCTTCATCCAG
TATCCAGTGTTGGTGCGACTGGAACAGGCAGAGGGCTTTCCTCAAGGAGATAATTCTACC
TCAGCACAAGGAAACTACCTGGAGGCCATCAATCTGTCATTCAATGTGTTTGATAAGCAC
TACATCAACCGCAACTTTGACCGAACTGGGCAGATGTCAGTGGTGATCACGCCCGGGGTG
GGTGTCTTTGAAGTGGACCGCCTACTCATGATCCTGACCAAGCAGCGGATGATAGATAAT
GGAATTGGTGTGGATTTGGTGTGCATGGGAGAGCAACCGTTACATGCTGTCCCATTGTTC
AAGCTCCATAATCGGAGTGCTCCCCGTGATTCTCGTCTGGGCGATGACTATAATATCCCT
CACTGGATAAACCACAGTTTCTACACATCCAAAAGCCAGCTCTTTTGTAATAGTTTCACC
CCACGAATAAAACTGGCAGGAAAGAAGCCCGCCTCTGAGAAAGCAAAAAATGGCCGTGAT
ACATCTCTCGGGAGTCCAAAAGAATCTGAGAACGCCCTTCCCATCCAAGTAGATTATGAC
GCCTATGACGCTCAAGTGTTCAGGCTGCCCGGCCCATCCCGGGCCCAGTGCCTCACCACC
TGCAGATCTGTGCGAGAGCGAGAGAGTCACAGTCGAAAGAGTGCCAGCTCCTGTGATGTT
TCATCCAGCCCTTCCCTACCAAGCCGCACACTGCCCACTGAGGAAGTGAGGAGCCAGGCT
TCTGACGACAGCTCCCTAGGCAAGAGTGCCAACATCCTGATGATCCCACACCCCCACCTG
CACCAGTATGAAGTCAGCAGCTCCTTGGGATACACCAGCACTCGAGATGTCCTGGAGAAC
ATGATGGAGCCACCACAGCGAGACTCCAGTGCACCAGGGAGGTTTCACGTTGGCAGTGCA
GAATCCATGCTGCATGTTCGACCTGGTGGATACACGCCCCAGAGAGCACTGATTAACCCC
TTCGCTCCCTCTCGGATGCCCATGAAGCTTACGTCCAACAGAAGGCGCTGGATGCACACT
TTTCCTGTGGGGCCATCCGGAGAAGCCATCCAGATCCACCACCAGACCCGACAGAATATG
GCGGAGCTACAAGGCAGCGGGCAGAGGGATCCAACTCACTCCTCTGCAGAGCTGCTGGAG
TTAGCATATCATGAAGCTGCTGGAAGGCACAGCAATTCCCGCCAGCCTGGTGACGGCATG
TCCTTCTTGAACTTCAGTGGAACAGAGGAGCTTTCTGTCGGCCTGCTTAGCAACAGTGGT
GCAGGTATGAATCCTAGGACCCAGAATAAGGATTCTCTAGAGGACAGTGTTTCTACCTCT
CCAGACCCAATTCTGACACTGTCTGCTCCCCCTGTAGTGCCAGGCTTCTGTTGCACAGTT
GGAGTGGACTGGAAGTCTCTCACTACTCCGGCGTGCCTCCCCCTTACCACCGACTACTTC
CCTGACCGCCAGGGCCTGCAGAATGACTACACAGAGGGCTGTTATGATCTCCTTCCAGAA
GCAGACATCGACAGGAGGGACGAAGATGGTGTGCAGATGACAGCCCAGCAGGTATTTGAA
GAGTTTATTTGCCAACGTCTCATGCAGGGCTACCAAATCATAGTGCAGCCCAAGACACAG
AAACCCAATCCTGCTGTCCCGCCCCCGCTGAGCAGTAGCCCACTCTATAGCCGAGGCCTT
GTGTCCCGAAACCGCCCTGAGGAGGAGGACCAGTATTGGCTGAGTATGGGCAGAACGTTC
CACAAAGTGACGCTGAAGGATAAGATGATCACAGTGACGCGATACCTTCCCAAGTATCCT
TATGAATCTGCCCAGATCCACTACACCTACAGCCTCTGTCCTTCCCACTCAGACTCAGAG
TTCGTCTCCTGCTGGGTGGAATTCTCCCACGAACGGCTGGAGGAGTACAAGTGGAATTAC
TTAGATCAGTATATCTGTTCTGCCGGCTCTGAAGACTTCAGCTTAATTGAGTCCCTGAAG
TTCTGGAGGACCCGCTTCCTGCTGCTGCCAGCCTGTGTCACCGCCACCAAGCGCATCACG
GAGGGGGAGGCCCACTGCGACATCTATGGGGACAGGCCCCGTGCAGACGAGGACGAGTGG
CAACTCCTGGATGGTTTTGTCCGCTTTGTGGAGGGCTTGAATCGCATTCGCAGGCGGCAT
CGCTCGGATCGCATGATGCGGAAAGGGACCGCCATGAAAGGCTTGCAGATGACTGGGCCC
ATTTCCACGCATTCTCTGGAGTCAACTGCACCCCCAGTGGGGAAGAAGGGAACCTCAGCT
CTCTCTGCCCTGTTGGAGATGGAGGCCAGTCAGAAGTGCCTGGGAGAACAGCAGGCAGCT
GTGCATGGTGGGAAGAGCTCCGCCCAGTCAGCCGAGAGCAGCAGCGTTGCCATGACTCCC
ACCTACATGGACAGCCCACGAAAGGACGGGGCCTTCTTTATGGAGTTTGTCCGCAGCCCA
CGCACAGCATCGTCCGCCTTCTACCCTCAGGTATCTGTGGACCAAACAGCCACTCCTATG
TTGGACGGCACCAGTTTGGGCATATGCACAGGCCAATCCATGGACAGAGGCAACAGCCAG
ACCTTTGGGAACTCCCAGAACATAGGAGAACAGGGCTACTCCTCCACAAACTCCAGTGAC
AGCAGCTCTCAGCAGCTGGTGGCAAGCTCCTTGACCTCATCCTCTACCCTGACAGAGATC
CTGGAAGCCATGAAGCACCCCTCGACAGGAGTCCAGCTGCTCTCTGAACAGAAGGGCCTC
TCACCGTACTGCTTCATCAGCGCGGAGGTGGTACACTGGTTGGTGAACCACGTGGAGGGG
ATCCAGACACAGGCGATGGCCATTGACATCATGCAGAAAATGCTGGAAGAGCAGCTCATC
ACACATGCATCTGGCGAAGCCTGGCGGACCTTCATCTACGGCTTCTATTTCTACAAGATA
GTAACGGACAAAGAGCCCGACCGAGTGGCCATGCAGCAGCCCGCCACCACCTGGCACACA
GCAGGAGTGGACGACTTCGCCAGCTTCCAGCGCAAGTGGTTTGAGGTGGCCTTTGTGGCA
GAAGAGCTCGTGCACTCTGAGATTCCTGCCTTTCTCCTGCCCTGGCTGCCTAGCCGGCCA
GCCTCCTATGCAAGTAGGCACAGCTCCTTTAGCCGAAGTTTTGGAGGACGGAGCCAGGCG
GCAGCACTTTTAGCTGCCACTGTCCCAGAGCAGAGGACTGTGACCCTGGATGTTGACGTG
AACAACCGCACAGACCGGCTGGAGTGGTGCAGCTGTTATTACCATGGCAACTTTTCTCTG
AATGCAGCCTTTGAGATCAAGCTGCACTGGATGGCGGTGACCGCAGCAGTACTCTTCGAG
ATGGTCCAAGGTTGGCATCGGAAAGCCACCTCCTGTGGCTTCTTGTTAGTCCCAGTTTTG
GAGGGGCCTTTTGCACTGCCCAGTTACCTGTATGGCGACCCCCTTCGTGCCCAGCTCTTC
ATCCCACTCAACATCAGCTGCTTGCTCAAGGAGGGCAGCGAGCACCTGTTTGATAGCTTT
GAACCCGAAACGTACTGGGATCGAATGCACCTCTTCCAGGAAGCCATTGCACACAGGTTT
GGGTTTGTACAAGATAAATATTCTGCCTCTGCTTTTAACTTCCCTGCTGAGAACAAGCCT
CAGTATATCCACGTTACAGGAACAGTGTTTCTGCAGCTGCCCTACTCCAAGCGCAAGTTC
TCAGGGCAGCAGCGGCGGCGGCGGAACTCCACCAGCTCCACCAACCAGAACATGTTCTGC
GAGGAGCGGGTCGGCTACAACTGGGCCTACAACACCATGCTCACCAAAACATGGCGCTCC
AGCGCCACAGGGGATGAAAAGTTTGCTGATCGGCTGCTGAAGGACTTCACGGACTTCTGC
ATCAACCGTGACAACCGGCTGGTCACGTTCTGGACAAGTTGCCTGGAGAAGATGCATGCC
AGTGCCCCGTTG
Protein sequence predicted based on nucleotide sequence
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKE DLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKS LVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCE MWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRA SIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNST SAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDN GIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFT PRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTT CRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHL HQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINP FAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLE LAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTS PDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPE ADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGL VSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSE FVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRIT EGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGP ISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTP TYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQ TFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGL SPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKI VTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRP ASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSL NAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLF IPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKP QYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRS SATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
