Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868564
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5799nts
Open reading frame: 1 to 5799
ATGGAGCTCACCCCTGCCTCTTCGCTGACTTGCTCCTTGCTCAGCCCGCGGCTGCCTGGC
TCTTTCCCCCAGCTGCGGAGGGTTCCTCCTTGCAGCCGGCCCTGGCTGCCCAAGGCCCCC
GTGATGGCTCGCTGGCCTCCCTTCGGCCTCTGCCTCCTCCTGCTGCTGCTGTCCCCACCG
CCACTGCCCTTGACAGGGGCCCATCGCTTCTCCGCACCTAATACCACTCTCAACCACTTG
GCACTGGCACCTGGCCGAGGCACACTCTATGTCGGCGCAGTGAACCGCCTCTTCCAGCTC
AGCCCCGAGCTGCAGCTCGAGGCCGTGGCTGTCACTGGCCCTGTAATCGACAGCCCTGAC
TGCGTGCCCTTCCGTGACCCAGCCGAGTGCCCACAGGCCCAGCTCACTGACAATGCCAAC
CAGCTGCTGCTGGTGAGCAGCCGCGCCCAGGAGCTGGTGGCCTGCGGGCAGGTGCGGCAG
GGCGTGTGTGAGACACGGCGCCTTGGGGATGTGGCCGAGGTGCTGTACCAGGCTGAGGAC
CCTGGTGACGGGCAGTTTGTGGCTGCCAATACCCCGGGAGTGGCAACGGTGGGGCTGGTG
GTGCCCTTGCCCGGCCGGGACCTCCTGCTTGTGGCCAGAGGCCTGGCGGGCAAGCTGTCG
GCAGGGGTGCCACCCCTGGCCATCCGCCAGCTGGCCGGGTCTCAGCCCTTCTCCAGCGAG
GGCCTGGGCCGCCTGGTGGTGGGCGACTTCTCCGACTACAACAACAGCTACGTCGGGGCC
TTTGCCGACGCCCGCTCCGCCTACTTCGTGTTCCGCCGCCGCGGGGCCCGGGCCCAGGCT
GAGTACCGCTCCTACGTGGCCCGCGTCTGCCTGGGGGACACCAACCTGTACTCCTACGTG
GAGGTCCCCCTCGCCTGCCAGGGCCAGGGCCTCATCCAGGCCGCCTTCCTTGCCCCGGGC
ACCTTGCTAGGGGTGTTTGCCGCGGGCCCAAGGGGCACCCAGGCGGCGCTCTGTGCCTTC
CCCATGGTGGAGCTGGGTGCCAGCATGGAGCAGGCCCGGAGACTCTGCTACACGGCGGGC
GGCCGGGGCCCCAGCGGCGCAGAGGAAGCCACCGTGGAGTACGGCGTCACGTCGCGCTGC
GTCACCCTGCCCCTTGATTCCCCCGAGTCGTACCCCTGTGGCGACGAGCACACCCCCAGC
CCCATTGCTGGCCGCCAGCCCCTGGAGGTCCAGCCTCTGCTGAAGCTCGGGCAGCCGGTC
AGCGCCGTGGCAGCTCTCCAGGCAGATGGGCACATGATAGCCTTCCTGGGGGACACCCAG
GGCCAGCTGTACAAGGTCTTTCTCCACGGCTCCCAGGGCCAGGTTTACCACTCCCAGCAA
GTGGGGCCTCCAGGCTCAGCCATCAGCCCAGACCTGCTGCTGGACAGCAGTGGCAGTCAC
CTCTATGTCCTGACTGCCCACCAGGTGGACCGGATACCTGTGGCAGCCTGCCCCCAGTTC
CCTGACTGTGCCAGCTGCCTCCAGGCCCAGGACCCGCTGTGTGGCTGGTGTGTCCTCCAG
GGCAGGTGTACCCGGAAGGGCCAGTGCGGGCGGGCAGGCCAGCTGAACCAGTGGCTGTGG
AGTTATGAGGAGGACAGCCACTGCCTGCACATCCAGAGCCTGCTGCCGGGCCACCACCCC
CGCCAGGAGCAGGGCCAGGTCACTTTGTCTGTCCCCCGGCTGCCCATCCTGGATGCAGAT
GAATACTTCCATTGTGCGTTCGGGGACTATGACAGCTTGGCTCATGTGGAAGGGCCCCAC
GTGGCCTGTGTCACCCCTCCCCAAGACCAGGTGCCACTTAACCCTCCAGGCACAGACCAC
GTCACTGTGCCCCTGGCCCTGATGTTCGAGGACGTGACTGTGGCTGCCACCAACTTCTCC
TTTTATGACTGCAGTGCCGTCCAGGCCTTGGAGGCGGCTGCCCCGTGTCGCGCTTGCGTG
GGCAGCATCTGGCGGTGTCACTGGTGCCCGCAGAGTAGCCACTGCGTGTACGGAGAGCAC
TGCCCAGAGGGCGAGAGGACCATCTACAGCGCCCAGGAGGTGGACATCCAGGTGCGTGGC
CCAGGGGCTTGCCCACAGGTCGAAGGCCTGGCAGGTCCCCACCTGGTGCCTGTGGGCTGG
GAGAGCCATTTGGCCCTACGCGTGCGGAACCTTCAACATTTCCGAGGCCTGCCTGCCTCC
TTCCACTGCTGGCTGGAGCTGCCTGGAGAACTTCGGGGACTGCCGGCCACCCTGGAGGAG
ACAGCAGGGGATTCAGGCCTCATCCACTGCCAGGCCCACCAGTTTTATCCCTCCATGTCC
CAGCGGGAGCTCCCAGTGCCCATCTACGTCACCCAGGGTGAAGCCCAGAGGCTGGACAAC
ACCCATGCTCTTTATGTGATCCTGTACGACTGCGCCATGGGCCACCCGGACTGCAGCCAC
TGCCAAGCGGCCAACAGGAGCCTGGGCTGCCTGTGGTGTGCTGACGGCCAGCCTGCCTGT
CGCTATGGGCCCTTGTGCCCGCCGGGGGCTGTGGAGCTGCTGTGTCCTGCGCCCAGCATT
GATGCAGTCGAGCCCCTGACCGGTCCCCCTGAGGGAGGCTTGGCCCTCACCATCCTGGGC
TCCAACCTGGGCCGGGCCTTCGCCGATGTGCAGTACGCCGTGAGCGTGGCCAGCCGGCCC
TGCAACCCTGAGCCCTCTCTCTACCGCACGTCGGCCCGGATTGTGTGTGTGACATCTCCT
GCCCCCAATGGCACCACTGGGCCCGTCCGGGTGGCCATTAAGAGCCAGCCACCAGGCATC
TCAAGCCAGCACTTCACCTACCAGGACCCTGTCCTGCTGAGCCTGAGTCCTCGCTGGGGC
CCCCAGGCAGGGGGCACCCAGCTCACCATCCGAGGTCAGCACCTCCAGACAGGTGGCAAC
ACCAGTGCCTTCGTGGGTGGCCAACCCTGTCCCATCCTGGAGCCAGTGTGTCCGGAGGCC
ATCGTGTGCCGTACCAGGCCCCAGGCTGCCCCAGGAGAAGCAGCGGTCCTTGTGGTCTTT
GGCCATGCCCAGCGCACACTGCTCGCCAGCCCCTTCCGCTACACCGCCAACCCCCAGCTT
GTAGCGGCGGAGCCCAGTGCCAGCTTCCGGGGGGGTGGGCGACTGATCCGTGTCAGGGGC
ACCGGCCTAGACGTGGTGCAGCGGCCCCTACTGTCTGTGTGGCTGGAGGCTGACGCAGAG
GTGCAGGCTTCCAGGGCCCAGCCCCAGGACCCACAGCCAAGGAGGAGCTGTGGAGCCCCT
GCTGCGGACCCCCAGGCTTGTATCCAGCTCGGTGGGGGGCTGCTGCAGTGCTCCACCGTC
TGCTCCGTCAACTCGTCCAGCCTCCTCCTGTGCCGGAGCCCTGCTGTACCAGACAGAGCC
CACCCGCAGCGGGTCTTCTTCACCCTAGACAACGTGCAAGTGGACTTCGCCAGTGCCAGT
GGGGGCCAGGGCTTCCTGTACCAGCCCAACCCCCGCCTGGCACCCCTCAGCCGCGAGGGG
CCTGCCCGCCCCTACCGCCTCAAGCCAGGCCATGTCCTGGATGTGGAGGGCGAGGGCCTC
AACCTGGGCATCAGCAAGGAGGAGGTGCGCGTGCACATCGGCCGCGGCGAGTGCCTGGTG
AAGACGCTCACGCGCACCCACCTGTACTGCGAGCCGCCTGCGCACGCCCCGCAGCCTGCC
AATGGCTCCGGCCTGCCACAGTTCGTGGTGCAGATGGGCAATGTGCAGCTGGCCCTGGGC
CCTGTGCAGTACGAGGCTGAACCCCCGCTGTCTGCCTTTCCCGTGGAGGCCCAGGCAGGC
GTGGGCATGGGTGCTGCAGTGCTGATTGCCGCCGTGCTCCTCCTCACCCTCATGTACAGG
CACAAGAGCAAGCAGGCCCTGCGGGACTACCAGAAGGTGCTAGTGCAGCTGGAGAGCCTG
GAGACCGGCGTGGGAGACCAGTGCCGCAAGGAGTTCACAGACCTCATGACGGAGATGACC
GACCTCAGCAGCGACCTGGAGGGCAGCGGGATCCCCTTCCTGGACTACCGCACCTACGCC
GAGCGCGCCTTCTTCCCTGGCCATGGCGGTTGCCCGCTGCAGCCCAAGCCTGAGGGGCCA
GGGGAGGACGGCCACTGTGCCACTGTGCGCCAGGGCCTCACGCAGCTCTCCAACCTGCTC
AACAGCAAGCTCTTCCTCCTCACGCTCATCCACACCCTGGAGGAGCAGCCCAGCTTTTCC
CAGAGGGATCGCTGCCATGTGGCTTCGCTGCTGTCGCTAGCGCTACACGGCAAGCTGGAG
TACCTGACGGACATCATGAGGACCCTGCTGGGTGACCTGGCGGCCCATTACGTGCACAGG
AACCCCAAGCTCATGCTACGCAGGACAGAGACCATGGTGGAGAAACTGCTCACCAACTGG
CTGTCCATCTGCCTGTACGCCTTCCTGAGGGAGGTGGCTGGTGAACCACTGTACATGCTC
TTCCGGGCCATCCAGTACCAGGTGGACAAAGGCCCCGTGGACGCCGTGACAGGCAAGGCC
AAACGGACCCTGAATGATAGCCGCTTGCTGCGGGAGGACGTGGAGTTCCAGCCCCTGACG
CTGATGGTGCTGGTGGGGCCCGGGGCTGGCGGGGCCGCAGGCAGCAGCGAGATGCAGCGC
GTGCCAGCCCGGGTGCTCGACACGGACACCATCACCCAGGTCAAGGAGAAGGTGTTGGAC
CAAGTCTACAAGGGCACCCCCTTCTCCCAGAGGCCCTCAGTGCATGCCCTAGACCTTGAG
TGGCGCTCAGGCCTGGCTGGTCACCTGACCCTATCGGACGAAGACTTGACCTCCGTGACC
CAAAACCACTGGAAGAGACTCAACACCTTGCAACACTACAAGGTCCCAGATGGAGCAACA
GTGGGGCTCGTCCCTCAGCTGCACCGTGGCAGCACCATCTCCCAGAGCCTGGCCCAGAGG
TGCCCCTTGGGAGAGAACATACCCACGCTGGAGGATGGCGAGGAGGGGGGGGTGTGCCTC
TGGCACCTGGTGAAAGCCACCGAGGAGCCAGAAGGGGCCAAGGTGCGGTGCAGCAGCCTG
CGGGAGCGCGAGCCAGCAAGGGCCAAGGCCATTCCGGAAATCTACCTCACCCGTCTGCTG
TCCATGAAGGGCACGCTGCAGAAGTTTGTGGACGACACCTTCCAGGCCATTCTCAGCGTG
AACCGGCCCATCCCCATCGCCGTCAAGTACCTGTTTGACCTTCTGGATGAGCTAGCAGAG
AAGCACGGCATCGAGGACCCAGGGACCCTGCACATCTGGAAGACCAACAGTCTGCTGCTG
CGGTTCTGGGTGAATGCCTTGAAGAACCCACAGCTCATCTTTGATGTACGGGTGTCGGAC
AATGTGGACGCCATCCTTGCTGTCATCGCCCAGACCTTCATTGACTCCTGTACCACCTCG
GAGCATAAAGTGGGCCGGGATTCCCCAGTGAACAAACTGCTCTACGCCCGGGAGATCCCA
CGCTACAAGCAGATGGTGGAGAGGTACTATGCGGACATTCGCCAGAGCTCTCCGGCGAGC
TACCAGGAGATGAACTCTGCTTTGGCTGAGCTCTCCGGGAACTACACTTCTGCTCCCCAC
TGTCTGGAGGCTCTGCAAGAACTCTACAACCACATCCACAGGTACTATGATCAGATTATC
AGTGCCCTGGAGGAGGACCCTGTGGGCCAGAAGCTGCAGCTGGCCTGCCGCCTGCAGCAG
GTCGCCGCCCTGGTGGAAAACAAAGTGACTGACCTGTTG
Protein sequence predicted based on nucleotide sequence
MELTPASSLTCSLLSPRLPGSFPQLRRVPPCSRPWLPKAPVMARWPPFGLCLLLLLLSPP PLPLTGAHRFSAPNTTLNHLALAPGRGTLYVGAVNRLFQLSPELQLEAVAVTGPVIDSPD CVPFRDPAECPQAQLTDNANQLLLVSSRAQELVACGQVRQGVCETRRLGDVAEVLYQAED PGDGQFVAANTPGVATVGLVVPLPGRDLLLVARGLAGKLSAGVPPLAIRQLAGSQPFSSE GLGRLVVGDFSDYNNSYVGAFADARSAYFVFRRRGARAQAEYRSYVARVCLGDTNLYSYV EVPLACQGQGLIQAAFLAPGTLLGVFAAGPRGTQAALCAFPMVELGASMEQARRLCYTAG GRGPSGAEEATVEYGVTSRCVTLPLDSPESYPCGDEHTPSPIAGRQPLEVQPLLKLGQPV SAVAALQADGHMIAFLGDTQGQLYKVFLHGSQGQVYHSQQVGPPGSAISPDLLLDSSGSH LYVLTAHQVDRIPVAACPQFPDCASCLQAQDPLCGWCVLQGRCTRKGQCGRAGQLNQWLW SYEEDSHCLHIQSLLPGHHPRQEQGQVTLSVPRLPILDADEYFHCAFGDYDSLAHVEGPH VACVTPPQDQVPLNPPGTDHVTVPLALMFEDVTVAATNFSFYDCSAVQALEAAAPCRACV GSIWRCHWCPQSSHCVYGEHCPEGERTIYSAQEVDIQVRGPGACPQVEGLAGPHLVPVGW ESHLALRVRNLQHFRGLPASFHCWLELPGELRGLPATLEETAGDSGLIHCQAHQFYPSMS QRELPVPIYVTQGEAQRLDNTHALYVILYDCAMGHPDCSHCQAANRSLGCLWCADGQPAC RYGPLCPPGAVELLCPAPSIDAVEPLTGPPEGGLALTILGSNLGRAFADVQYAVSVASRP CNPEPSLYRTSARIVCVTSPAPNGTTGPVRVAIKSQPPGISSQHFTYQDPVLLSLSPRWG PQAGGTQLTIRGQHLQTGGNTSAFVGGQPCPILEPVCPEAIVCRTRPQAAPGEAAVLVVF GHAQRTLLASPFRYTANPQLVAAEPSASFRGGGRLIRVRGTGLDVVQRPLLSVWLEADAE VQASRAQPQDPQPRRSCGAPAADPQACIQLGGGLLQCSTVCSVNSSSLLLCRSPAVPDRA HPQRVFFTLDNVQVDFASASGGQGFLYQPNPRLAPLSREGPARPYRLKPGHVLDVEGEGL NLGISKEEVRVHIGRGECLVKTLTRTHLYCEPPAHAPQPANGSGLPQFVVQMGNVQLALG PVQYEAEPPLSAFPVEAQAGVGMGAAVLIAAVLLLTLMYRHKSKQALRDYQKVLVQLESL ETGVGDQCRKEFTDLMTEMTDLSSDLEGSGIPFLDYRTYAERAFFPGHGGCPLQPKPEGP GEDGHCATVRQGLTQLSNLLNSKLFLLTLIHTLEEQPSFSQRDRCHVASLLSLALHGKLE YLTDIMRTLLGDLAAHYVHRNPKLMLRRTETMVEKLLTNWLSICLYAFLREVAGEPLYML FRAIQYQVDKGPVDAVTGKAKRTLNDSRLLREDVEFQPLTLMVLVGPGAGGAAGSSEMQR VPARVLDTDTITQVKEKVLDQVYKGTPFSQRPSVHALDLEWRSGLAGHLTLSDEDLTSVT QNHWKRLNTLQHYKVPDGATVGLVPQLHRGSTISQSLAQRCPLGENIPTLEDGEEGGVCL WHLVKATEEPEGAKVRCSSLREREPARAKAIPEIYLTRLLSMKGTLQKFVDDTFQAILSV NRPIPIAVKYLFDLLDELAEKHGIEDPGTLHIWKTNSLLLRFWVNALKNPQLIFDVRVSD NVDAILAVIAQTFIDSCTTSEHKVGRDSPVNKLLYAREIPRYKQMVERYYADIRQSSPAS YQEMNSALAELSGNYTSAPHCLEALQELYNHIHRYYDQIISALEEDPVGQKLQLACRLQQ VAALVENKVTDLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
