Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868705
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7092nts
Open reading frame: 1 to 7092
ATGGCGGCGGTGTTGCAGCAAGTCCTGGAGCGCACGGAGCTGAACAAGCTGCCCAAGTCT
GTCCAGAACAAACTTGAAAAGTTCCTTGCTGATCAGCAATCCGAGATCGATGGCCTGAAG
GGGCGGCATGAGAAATTTAAGGTGGAGAGCGAACAACAGTATTTTGAAATAGAAAAGAGG
TTGTCCCACAGTCAGGAGAGACTTGTGAATGAAACCCGAGAGTGTCAAAGCTTGCGGCTT
GAGCTAGAGAAACTCAACAATCAACTGAAGGCACTAACTGAGAAAAACAAAGAACTTGAA
ATTGCTCAGGATCGCAATATTGCCATTCAGAGCCAATTTACAAGAACAAAGGAAGAATTA
GAAGCTGAGAAAAGAGACTTAATTAGAACCAATGAGAGACTATCTCAAGAACTTGAATAC
TTAACAGAGGATGTTAAACGTCTGAATGAAAAACTTAAAGAAAGCAATACAACAAAGGGT
GAACTTCAGTTAAAATTGGATGAACTTCAAGCTTCTGATGTTTCTGTTAAGTATCGAGAA
AAACGCTTGGAGCAAGAAAAGGAATTGCTACATAGTCAGAATACATGGCTGAATACAGAG
TTGAAAACCAAAACTGATGAACTTCTGGCTCTTGGAAGAGAAAAAGGGAATGAGATTCTA
GAGCTTAAATGTAATCTTGAAAATAAAAAAGAAGAGGTTTCTAGACTGGAAGAACAAATG
AATGGCTTAAAAACATCAAATGAACATCTTCAAAAGCATGTGGAGGATCTGTTGACCAAA
TTAAAAGAGGCCAAGGAACAACAGGCCAGTATGGAAGAGAAATTCCACAATGAATTAAAT
GCCCACATAAAACTTTCTAATTTGTACAAGAGTGCCGCTGATGACTCAGAAGCAAAGAGC
AATGAACTAACCCGGGCAGTAGAGGAACTACACAAACTTTTGAAAGAAGCTGGTGAAGCC
AACAAAGCAATACAAGATCATCTTCTAGAGGTGGAGCAATCCAAAGATCAAATGGAAAAA
GAAATGCTTGAGAAAATAGGGAGATTGGAGAAGGAATTAGAGAATGCAAATGACCTTCTT
TCTGCCACAAAACGTAAAGGAGCCATATTGTCTGAAGAAGAGCTTGCCGCCATGTCTCCT
ACTGCAGCAGCTGTAGCTAAGATAGTGAAACCTGGGATGAAACTAACTGAGCTCTATAAT
GCTTATGTGGAAACTCAGGATCAGTTGCTTTTGGAGAAACTAGAGAACAAAAGAATTAAT
AAGTACCTAGATGAAATAGTGAAAGAAGTGGAAGCCAAAGCACCAATTTTGAAACGCCAG
CGTGAGGAATATGAACGTGCACAGAAAGCTGTAGCAAGTTTATCTGTTAAGCTTGAACAA
GCTATGAAGGAGATTCAGCGATTGCAGGAGGACACTGATAAAGCCAACAAGCAATCATCT
GTACTTGAGAGAGATAATCGAAGAATGGAAATACAAGTAAAAGATCTTTCACAACAGATT
AGAGTGCTTTTGATGGAACTTGAAGAAGCAAGGGGTAACCACGTAATTCGTGATGAGGAA
GTAAGCTCTGCTGATATAAGTAGTTCATCTGAGGTAATATCACAGCATCTAGTATCTTAC
AGAAATATTGAAGAGCTTCAACAACAAAATCAACGTCTCTTAGTGGCCCTTAGAGAGCTT
GGGGAAACCAGAGAAAGAGAAGAACAAGAAACAACTTCATCCAAAATCACTGAGCTTCAG
CTCAAACTTGAGAGTGCCCTTACTGAACTAGAACAACTCCGCAAATCACGACAGCATCAA
ATGCAGCTTGTTGATTCCATAGTTCGTCAGCGTGATATGTACCGTATTTTATTGTCACAA
ACAACAGGAGTTGCCATTCCATTACATGCTTCAAGCTTAGATGATGTTTCTCTTGCATCA
ACTCCAAAACGTCCAAGTACATCACAGACTGTTTCCACTCCTGCTCCAGTACCTGTTATT
GAATCAACAGAGGCTATAGAGGCTAAGGCTGCCCTTAAACAGTTGCAGGAAATTTTTGAG
AACTACAAAAAAGAAAAAGCAGAAAATGAAAAAATACAAAATGAGCAGCTTGAGAAACTT
CAAGAACAAGTTACAGATTTGCGATCACAAAATACCAAAATTTCTACCCAGCTAGATTTT
GCTTCTAAACGTTATGAAATGCTGCAAGATAATGTTGAAGGATATCGTCGAGAAATAACA
TCACTTCATGAGAGAAATCAGAAACTCACTGCCACAACTCAAAAGCAAGAACAGATTATC
AATACGATGACTCAAGATTTGAGAGGAGCAAATGAGAAGCTAGCTGTCGCAGAAGTAAGA
GCAGAAAATTTGAAGAAGGAAAAGGAAATGCTTAAATTGTCTGAAGTTCGTCTTTCTCAG
CAAAGAGAGTCTTTGTTAGCTGAACAAAGGGGGCAAAACTTACTGCTAACTAATCTGCAA
ACAATTCAGGGAATACTGGAGCGATCTGAAACAGAAACCAAACAAAGGCTTAGTAGCCAG
ATAGAAAAACTGGAACATGAGATCTCTCATCTAAAGAAGAAGTTGGAAAATGAGGTGGAA
CAAAGGCATACACTTACTAGAAATCTAGATGTTCAACTTTTAGATACAAAGAGACAACTG
GATACAGAGACAAATCTTCATCTTAACACAAAAGAACTATTAAAAAATGCTCAAAAAGAA
ATTGCCACATTGAAACAGCACCTCAGTAATATGGAAGTCCAAGTTGCTTCTCAGTCTTCA
CAGAGAACTGGTAAAGGTCAGCCTAGCAACAAAGAAGATGTGGATGATCTTGTGAGTCAG
CTAAGACAGACAGAAGAGCAGGTGAATGACTTAAAGGAGAGACTCAAAACAAGTACGAGC
AATGTGGAACAATATCAAGCAATGGTTACTAGTTTAGAAGAATCCCTGAACAAGGAAAAA
CAGGTGACAGAAGAAGTGCGTAAGAATATTGAAGTTCGTTTAAAAGAGTCAGCTGAATTT
CAGACACAGTTGGAAAAGAAGTTGATGGAAGTAGAGAAGGAAAAACAAGAACTTCAGGAT
GATAAAAGAAGAGCCATAGAGAGCATGGAACAACAGTTATCTGAATTGAAGAAAACACTT
TCTAGTGTTCAGAATGAAGTACAAGAAGCTCTTCAGAGAGCAAGCACAGCTTTAAGTAAT
GAGCAGCAAGCCAGACGTGACTGTCAGGAACAAGCTAAAATAGCTGTGGAAGCTCAGAAT
AAGTATGAGAGAGAATTGATGCTGCATGCTGCTGATGTTGAAGCTCTACAAGCTGCGAAG
GAGCAGGTTTCAAAAATGGCATCAGTCCGTCAGCATTTGGAAGAAACAACACAGAAAGCA
GAATCACAGTTGTTGGAGTGTAAAGCATCTTGGGAGGAAAGAGAGAGAATGTTAAAGGAT
GAAGTTTCCAAATGTGTATGTCGCTGTGAAGATCTGGAGAAACAAAACAGATTACTTCAT
GATCAGATCGAAAAATTAAGTGACAAGGTCGTTGCCTCTGTGAAGGAAGGTGTACAAGGT
CCACTGAATGTATCTCTCAGTGAAGAAGGAAAATCTCAAGAACAAATTTTGGAAATTCTC
AGATTTATACGACGAGAAAAAGAAATTGCTGAAACTAGGTTTGAGGTGGCTCAGGTTGAG
AGTCTGCGTTATCGACAAAGGGTTGAACTTTTAGAAAGAGAGCTGCAGGAACTGCAAGAT
AGTCTAAATGCTGAAAGGGAGAAAGTCCAGGTAACTGCAAAAACAATGGCTCAGCATGAA
GAACTGATGAAGAAAACTGAAACAATGAATGTAGTTATGGAGACCAATAAAATGCTAAGA
GAAGAGAAGGAGAGACTAGAACAGGATCTACAGCAAATGCAAGCAAAGGTGAGGAAACTG
GAGTTAGATATTTTACCCTTACAAGAAGCAAATGCTGAGCTGAGTGAGAAAAGCGGTATG
TTGCAGGCAGAGAAGAAGCTCTTAGAAGAGGATGTCAAACGTTGGAAAGCACGTAACCAG
CATCTAGTAAGTCAACAGAAAGATCCAGATACAGAAGAATATCGGAAGCTCCTTTCTGAA
AAGGAAGTTCATACTAAGCGTATTCAACAATTGACAGAAGAAATTGGTAGACTTAAAGCT
GAAATTGCAAGATCAAATGCATCTTTGACTAACAACCAGAACTTAATTCAGAGTCTGAAG
GAAGATCTAAATAAAGTAAGAACTGAAAAGGAAACCATCCAGAAGGACTTAGATGCCAAA
ATAATTGATATCCAAGAAAAAGTCAAAACTATTACTCAAGTTAAGAAAATTGGACGTAGG
TACAAGACTCAATATGAAGAACTTAAAGCACAACAGGATAAGGTTATGGAGACATCGGCT
CAGTCCTCTGGAGACCATCAGGAGCAGCATGTTTCAGTCCAGGAAATGCAGGAACTCAAA
GAAACGCTCAACCAAGCTGAAACAAAATCAAAATCACTTGAAAGTCAAGTAGAGAATCTG
CAGAAGACATTATCTGAAAAAGAGACAGAAGCAAGAAATCTCCAGGAACAGACTGTGCAA
CTTCAGTCTGAACTTTCACGACTTCGTCAGGATCTTCAAGATAGAACCACACAGGAGGAG
CAGCTCCGACAACAGATAACTGAAAAGGAAGAAAAAACCAGAAAGGCTATTGTAGCAGCA
AAGTCAAAAATTGCACACTTAGCTGGTGTAAAAGATCAGCTAACTAAAGAAAATGAGGAG
CTTAAACAAAGGAATGGAGCCTTAGATCAGCAGAAAGATGAATTGGATGTTCGCATTACT
GCGCTAAAGTCCCAATATGAAGGTCGAATTAGTCGCTTGGAAAGAGAACTCAGGGAGCAT
CAAGAGAGACACCTTGAGCAGAGAGATGAGCCTCAAGAACCTTCTAATAAGGTCCCTGAA
CAGCAGAGACAGATCACATTGAAAACAACTCCAGCTTCTGGTGAAAGAGGAATTGCCAGC
ACATCAGACCCACCAACAGCCAATATCAAGCCAACTCCTGTTGTGTCTACTCCAAGTAAA
GTGACAGCTGCAGCTATGGCTGGAAATAAGTCAACACCCAGGGCTAGTATCCGCCCAATG
GTTACACCTGCAACTGTTACAAATCCCACTACTACCCCAACAGCTACAGTGATGCCCACT
ACACAAGTGGAATCACAGGAAGCTATGCAGTCAGAAGGGCCTGTGGAACATGTTCCAGTT
TTTGGAAGCACAAGTGGATCCGTTCGTTCTACTAGTCCTAATGTCCAGCCTTCTATCTCT
CAACCTATTTTAACTGTTCAGCAACAAACACAGGCTACAGCTTTTGTGCAACCCACTCAA
CAGAGTCATCCTCAGATTGAGCCTGCCAATCAAGAGTTATCTTCAAACATAGTAGAGGTT
GTTCAGAGTTCACCAGTTGAGCGGCCTTCTACTTCCACAGCAGTATTTGGCACAGTTTCG
GCTACCCCCAGTTCTTCTTTGCCAAAGCGTACACGTGAAGAGGAAGAGGATAGCACCATA
GAAGCATCAGACCAAGTCTCTGATGATACAGTGGAAATGCCTCTTCCAAAGAAGTTGAAA
AGTGTCACACCTGTAGGAACTGAGGAAGAAGTTATGGCAGAAGAAAGTACTGATGGAGAG
GTAGAGACTCAGGTATACAACCAGGATTCTCAAGATTCCATTGGAGAAGGAGTTACCCAG
GGAGATTATACACCTATGGAAGACAGTGAAGAAACCTCTCAGTCTCTACAAATAGATCTT
GGGCCACTTCAATCAGATCAGCAGACGACAACTTCATCCCAGGATGGTCAAGGCAAAGGA
GATGATGTCATTGTAATTGACAGTGATGATGAAGAAGAGGATGATGATGAAAATGATGGA
GAACATGAGGATTATGAAGAGGATGAGGAAGATGATGATGATGATGAAGATGACACAGGG
ATGGGAGATGAGGGTGAAGATAGTAATGAAGGAACTGGTAGTGCCGATGGCAATGATGGT
TATGAAGCTGATGATGCTGAGGGTGGTGATGGGACTGATCCAGGTACAGAAACAGAAGAA
AGTATGGGTGGAGGTGAAGGTAATCACAGAGCTGCTGATTCTCAAAACAGTGGTGAAGGA
AATACAGGTGCTGCAGAATCTTCTTTTTCTCAGGAGGTTTCTAGAGAACAACAGCCATCA
TCAGCATCTGAAAGACAGGCCCCTCGAGCACCTCAGTCACCGAGACGCCCACCACATCCA
CTTCCCCCAAGACTGACCATTCATGCCCCACCTCAGGAGTTGGGACCACCAGTTCAGAGA
ATTCAGATGACCCGAAGGCAGTCTGTAGGACGTGGCCTTCAGTTGACTCCAGGAATAGGT
GGCATGCAACAGCATTTTTTTGATGATGAAGACAGAACAGTTCCAAGTACTCCAACTCTT
GTGGTGCCACATCGTACTGATGGATTTGCTGAAGCAATTCATTCGCCGCAGGTTGCTGGT
GTCCCTAGATTCCGGTTTGGGCCACCTGAAGATATGCCACAAACAAGTTCTAGTCACTCT
GATCTTGGCCAGCTTGCTTCTCAAGGAGGTTTAGGAATGTATGAAACACCCCTGTTCCTA
GCTCATGAAGAAGAGTCAGGTGGCCGAAGTGTTCCCACTACTCCACTACAAGTAGCAGCC
CCAGTGACTGTATTTACTGAGAGCACCACCTCTGATGCTTCGGAACATGCCTCTCAATCT
GTTCCAATGGTGACTACATCCACTGGCACTTTATCTACAACAAATGAAACAGCAACAGGT
GATGATGGAGATGAAGTATTTGTGGAGGCAGAATCTGAAGGTATTAGTTCAGAAGCAGGC
CTAGAAATTGATAGCCAGCAGGAAGAAGAGCCGGTTCAAGCATCTGATGAGTCAGATCTC
CCCTCCACCAGCCAGGATCCTCCTTCTAGCTCATCTGTAGATACTAGTAGTAGTCAACCA
AAGCCTTTCAGACGAGTAAGACTTCAGACAACATTGAGACAAGGTGTCCGTGGTCGTCAG
TTTAACAGACAGAGAGGTGTGAGCCATGCAATGGGAGGGAGAGGAGGAATAAACAGAGGA
AATATTAATTTG
Protein sequence predicted based on nucleotide sequence
MAAVLQQVLERTELNKLPKSVQNKLEKFLADQQSEIDGLKGRHEKFKVESEQQYFEIEKR LSHSQERLVNETRECQSLRLELEKLNNQLKALTEKNKELEIAQDRNIAIQSQFTRTKEEL EAEKRDLIRTNERLSQELEYLTEDVKRLNEKLKESNTTKGELQLKLDELQASDVSVKYRE KRLEQEKELLHSQNTWLNTELKTKTDELLALGREKGNEILELKCNLENKKEEVSRLEEQM NGLKTSNEHLQKHVEDLLTKLKEAKEQQASMEEKFHNELNAHIKLSNLYKSAADDSEAKS NELTRAVEELHKLLKEAGEANKAIQDHLLEVEQSKDQMEKEMLEKIGRLEKELENANDLL SATKRKGAILSEEELAAMSPTAAAVAKIVKPGMKLTELYNAYVETQDQLLLEKLENKRIN KYLDEIVKEVEAKAPILKRQREEYERAQKAVASLSVKLEQAMKEIQRLQEDTDKANKQSS VLERDNRRMEIQVKDLSQQIRVLLMELEEARGNHVIRDEEVSSADISSSSEVISQHLVSY RNIEELQQQNQRLLVALRELGETREREEQETTSSKITELQLKLESALTELEQLRKSRQHQ MQLVDSIVRQRDMYRILLSQTTGVAIPLHASSLDDVSLASTPKRPSTSQTVSTPAPVPVI ESTEAIEAKAALKQLQEIFENYKKEKAENEKIQNEQLEKLQEQVTDLRSQNTKISTQLDF ASKRYEMLQDNVEGYRREITSLHERNQKLTATTQKQEQIINTMTQDLRGANEKLAVAEVR AENLKKEKEMLKLSEVRLSQQRESLLAEQRGQNLLLTNLQTIQGILERSETETKQRLSSQ IEKLEHEISHLKKKLENEVEQRHTLTRNLDVQLLDTKRQLDTETNLHLNTKELLKNAQKE IATLKQHLSNMEVQVASQSSQRTGKGQPSNKEDVDDLVSQLRQTEEQVNDLKERLKTSTS NVEQYQAMVTSLEESLNKEKQVTEEVRKNIEVRLKESAEFQTQLEKKLMEVEKEKQELQD DKRRAIESMEQQLSELKKTLSSVQNEVQEALQRASTALSNEQQARRDCQEQAKIAVEAQN KYERELMLHAADVEALQAAKEQVSKMASVRQHLEETTQKAESQLLECKASWEERERMLKD EVSKCVCRCEDLEKQNRLLHDQIEKLSDKVVASVKEGVQGPLNVSLSEEGKSQEQILEIL RFIRREKEIAETRFEVAQVESLRYRQRVELLERELQELQDSLNAEREKVQVTAKTMAQHE ELMKKTETMNVVMETNKMLREEKERLEQDLQQMQAKVRKLELDILPLQEANAELSEKSGM LQAEKKLLEEDVKRWKARNQHLVSQQKDPDTEEYRKLLSEKEVHTKRIQQLTEEIGRLKA EIARSNASLTNNQNLIQSLKEDLNKVRTEKETIQKDLDAKIIDIQEKVKTITQVKKIGRR YKTQYEELKAQQDKVMETSAQSSGDHQEQHVSVQEMQELKETLNQAETKSKSLESQVENL QKTLSEKETEARNLQEQTVQLQSELSRLRQDLQDRTTQEEQLRQQITEKEEKTRKAIVAA KSKIAHLAGVKDQLTKENEELKQRNGALDQQKDELDVRITALKSQYEGRISRLERELREH QERHLEQRDEPQEPSNKVPEQQRQITLKTTPASGERGIASTSDPPTANIKPTPVVSTPSK VTAAAMAGNKSTPRASIRPMVTPATVTNPTTTPTATVMPTTQVESQEAMQSEGPVEHVPV FGSTSGSVRSTSPNVQPSISQPILTVQQQTQATAFVQPTQQSHPQIEPANQELSSNIVEV VQSSPVERPSTSTAVFGTVSATPSSSLPKRTREEEEDSTIEASDQVSDDTVEMPLPKKLK SVTPVGTEEEVMAEESTDGEVETQVYNQDSQDSIGEGVTQGDYTPMEDSEETSQSLQIDL GPLQSDQQTTTSSQDGQGKGDDVIVIDSDDEEEDDDENDGEHEDYEEDEEDDDDDEDDTG MGDEGEDSNEGTGSADGNDGYEADDAEGGDGTDPGTETEESMGGGEGNHRAADSQNSGEG NTGAAESSFSQEVSREQQPSSASERQAPRAPQSPRRPPHPLPPRLTIHAPPQELGPPVQR IQMTRRQSVGRGLQLTPGIGGMQQHFFDDEDRTVPSTPTLVVPHRTDGFAEAIHSPQVAG VPRFRFGPPEDMPQTSSSHSDLGQLASQGGLGMYETPLFLAHEEESGGRSVPTTPLQVAA PVTVFTESTTSDASEHASQSVPMVTTSTGTLSTTNETATGDDGDEVFVEAESEGISSEAG LEIDSQQEEEPVQASDESDLPSTSQDPPSSSSVDTSSSQPKPFRRVRLQTTLRQGVRGRQ FNRQRGVSHAMGGRGGINRGNINL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
