Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868729
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7746nts
Open reading frame: 1 to 7746
ATGGCAGACCCCATCATGGATCTGTTCGATGACCCAAATTTATTTGGCCTGGACTCTCTG
ACTGATGACAGCTTTAACCAGGTCACACAAGACCCCATTGAGGAAGCCCTTGGACTGCCA
AGCTCTCTGGACTCCTTGGATCAGATGAACCAGGATGGTGGAGGTGGTGATGTGGGGAAT
TCATCAGCAAGTGAACTGGTCCCTCCACCAGAGGAAACAGCTCCCACAGAACTTTCCAAA
GAATCCACAGCTCCAGCTCCAGAATCCATAACCTTGCATGATTATACCACTCAGCCTGCC
AGCCAGGAGCAGCCAGCCCAACCTGTCTTACAGACATCGACGCCAACATCAGGACTTTTG
CAAGTCTCCAAGAGCCAGGAGATCCTGAGCCAAGGGAATCCTTTCATGGGTGTCTCTGCC
ACAGCTGTCTCCTCCAGTAGTGCTGGAGGGCAGCCACCTCAGTCAGCCCCTAAGATTGTT
ATCCTTAAGGCCCCACCAAGCTCCTCAGTCACTGGTGCCCATGTGGCACAAATTCAGGCC
CAAGGTATCACCAGCACAGCTCAGCCCCTGGTGGCAGGCACAGCCAATGGTGGAAAAGTC
ACTTTTACCAAAGTGCTAACCGGCACACCCCTTCGACCAGGTGTTTCCATTGTCTCTGGT
AATACAGTGTTGGCCGCCAAGGTCCCTGGGAACCAGGCTGCTGTTCAGCGCATTGTCCAG
CCCAGCCGACCAGTAAAGCAGCTGGTCCTCCAGCCAGTTAAGGGTTCAGCTCCTGCTGGA
AACCCTGGGGCCACAGGGCCCCCACTGAAGCCTGCAGTTACACTGACCTCTACACCTACC
CAGGGTGAATCGAAACGCATCACCCTGGTCCTCCAGCAGCCACAGTCTGGAGGTCCCCAA
GGACATCGGCATGTTGTGCTAGGGAGTCTACCAGGCAAGATAGTGTTACAGGGCAACCAG
CTGGCAGCCCTGACTCAAGCCAAGAATGCCCAAGGGCAGCCTGCCAAGGTAGTAACTATC
CAGCTGCAGGTGCAGCAGCCACAGCAAAAAATCCAGATTGTACCACAACCACCATCATCG
CAGCCACAGCCCCAGCAGCCACCCTCCACCCAGCCAGTGACTCTGTCCTCTGTACAGCAG
GCTCAGATAATGGGACCAGGACAAAGCCCAGGACAAAGACTTTCAGTACCAGTCAAGGTG
GTACTGCAGCCACAGGCTGGCTCTTCCCAAGGGGCCTCTTCTGGGCTCTCTGTAGTTAAA
GTTCTGAGTGCCAGTGAAGTGGCAGCTTTGTCATCACCAGCAAGCTCTGCTCCTCATTCG
GGGGGAAAGACAGGAATGGAGGAAAACCGCAGATTGGAACACCAGAAGAAGCAAGAGAAA
GCAAATCGGATTGTAGCAGAGGCCATTGCGAGAGCCCGTGCCCGCGGTGAGCAGAACATA
CCTCGAGTCTTAAATGAGGACGAGTTGCCCAGCGTTCGGCCAGAGGAGGAAGGCGAGAAG
AAACGCAGGAAGAAGAGTGCTGGGGAGAGGCTGAAAGAGGAGAAGCCAAAGAAGAGTAAA
ACATCTGGTGCCTCCAAAACAAAGGGCAAGAGCAAGCTCAACACCATCACTCCTGTAGTG
GGTAAGAAGAGAAAACGTAATACCTCATCTGATAATTCAGATGTGGAAGTCATGCCTGCA
CAGTCACCTCGAGAAGATGAAGAAAGCAGCATTCAGAAGAGACGCTCAAACCGCCAAGTT
AAGCGAAAAAAATATACAGAGGACCTGGATATAAAGATCACAGATGATGAAGAAGAAGAA
GAGGTGGATGTAACTGGTCCAATAAAACCTGAGCCTATCCTCCCTGAACCAGTGCAAGAA
CCAGATGGCGAGACTTTGCCTTCCATGCAGTTCTTTGTGGAGAATCCCAGTGAAGAAGAT
GCAGCCATTGTAGACAAAGTGCTTTCTATGCGGATTGTGAAGAAGGAGCTCCCTTCTGGA
CAATATACTGAAGCAGAAGAATTCTTTGTCAAGTACAAGAACTACTCCTATCTGCATTGT
GAATGGGCTACTATCTCCCAACTAGAGAAGGATAAGAGGATACATCAAAAATTAAAGCGC
TTCAAAACCAAAATGGCTCAGATGAGACACTTCTTCCATGAGGATGAAGAGCCCTTTAAT
CCAGACTACGTAGAGGTGGATAGGATATTGGATGAGTCTCACAGTATTGACAAGGACAAT
GGGGAGCCCGTTATTTACTACCTGGTGAAATGGTGCTCTCTGCCCTATGAGGATAGCACA
TGGGAGCTAAAAGAAGATGTTGATGAGGGCAAGATTCGAGAATTTAAACGGATTCAGTCA
AGGCACCCAGAACTCAAAAGGGTGAATCGTCCGCAGGCAAGTGCCTGGAAGAAATTGGAG
CTATCACATGAATATAAAAACAGAAACCAGCTACGGGAATATCAGTTGGAAGGGGTTAAT
TGGCTGCTCTTTAATTGGTATAACAGGCAGAACTGCATCCTGGCTGATGAGATGGGATTG
GGCAAAACTATTCAGTCCATTGCCTTCTTGCAGGAAGTATATAATGTGGGCATCCATGGT
CCCTTCTTGGTCATTGCCCCACTGTCCACAATTACTAACTGGGAGCGAGAATTTAATACA
TGGACAGAAATGAACACTATTGTGTACCATGGCAGTCTGGCCAGCAGGCAGATGATTCAA
CAGTATGAAATGTACTGCAAAGATTCACGGGGACGCCTCATCCCAGGCGCATACAAGTTT
GACGCTCTGATCACCACTTTTGAGATGATTTTGTCAGATTGTCCTGAGCTTCGTGAAATT
GAATGGCGTTGTGTTATCATTGATGAAGCCCATCGACTGAAAAACCGTAATTGCAAGCTG
CTTGATAGTCTCAAGCACATGGACCTGGAACACAAGGTGCTACTCACAGGAACACCATTG
CAAAATACTGTAGAAGAACTGTTTAGCTTGCTTCATTTCTTGGAACCGTCACAATTTCCC
TCAGAATCAGAGTTTCTCAAGGACTTTGGGGATCTCAAGACAGAGGAACAGGTTCAAAAG
CTACAGGCCATTCTTAAGCCAATGATGCTGAGAAGACTCAAAGAGGATGTTGAAAAAAAC
TTGGCACCCAAACAGGAAACAATTATTGAAGTAGAGCTGACTAATATCCAGAAGAAATAC
TATCGGGCTATTTTGGAGAAGAATTTCTCCTTCCTTTCCAAAGGGGCAGGTCATACCAAC
ATGCCTAATCTACTTAACACAATGATGGAGTTGCGCAAGTGCTGCAACCACCCATATCTC
ATCAATGGTGCTGAAGAAAAAATCCTAACAGAATTCCGTGAAGCTTGCCATATTATACCT
CATGACTTTCACCTGCAGGCCATGGTTCGTTCAGCCGGCAAACTGGTTCTTATTGACAAG
TTGCTTCCAAAGCTTAAAGCTGGTGGCCATAAAGTTCTGATCTTCTCTCAGATGGTGCGC
TGCCTAGACATCCTAGAGGATTATTTAATCCAGAGGAGGTACTTATATGAACGTATTGAT
GGGCGAGTTAGAGGCAACCTTCGACAGGCTGCCATTGACCGCTTCAGCAAGCCTGACTCA
GACCGCTTTGTCTTCTTACTGTGTACCCGGGCTGGTGGACTTGGTATTAATCTTACAGCT
GCTGATACCTGCATCATCTTTGATTCAGACTGGAATCCACAAAATGACCTGCAGGCCCAG
GCACGATGTCATCGAATTGGGCAGAGCAAAGCTGTGAAGGTGTACCGCCTCATCACTCGT
AATTCCTACGAGAGAGAGATGTTTGATAAGGCCAGCCTCAAGTTGGGGTTGGATAAGGCT
GTGCTTCAATCCATGAGTGGTCGGGATGGCAACATTACTGGAATCCAACAGTTCTCTAAG
AAGGAGATTGAAGATCTTTTAAGAAAAGGAGCATATGCAGCCATCATGGAGGAAGATGAT
GAAGGCTCCAAGTTTTGTGAAGAGGACATTGACCAGATCTTGTTAAGACGAACTACAACC
ATCACCATTGAATCTGAAGGAAAAGGTTCCACCTTTGCTAAGGCAAGCTTTGTTGCTTCT
GAAAACAGGACAGATATTTCTTTGGATGACCCCAACTTTTGGCAAAAGTGGGCCAAAAAG
GCTGACCTAGACATGGATCTGCTCAACAGCAAGAATAATTTGGTAATTGACACACCTAGA
GTACGAAAACAAACGCGCCACTTTAGCACTCTGAAAGATGATGACCTGGTGGAATTCTCT
GATTTGGAAAGTGAGGATGATGAGCGGCCACGCTCCCGCAGACATGACCGTCATCATGCC
TATGGGCGCACTGACTGCTTTCGGGTGGAAAAGCATCTCCTGGTATATGGTTGGGGACGA
TGGCGAGATATTTTATCTCATGGACGCTTCAAGCGACGTATGACTGAACGAGATGTGGAG
ACCATTTGTCGGGCCATTCTCGTGTACTGTCTTCTACACTACCGTGGGGATGAAAATATT
AAAGGCTTCATCTGGGACTTGATTAGCCCAGCTGAAAATGGCAAGACAAAAGAATTGCAG
AATCATTCAGGTCTATCTATCCCTGTGCCTCGTGGACGCAAAGGAAAAAAAGTAAAGTCA
CAAAGCACTTTTGATATCCATAAGGCAGATTGGATCCGGAAATATAACCCTGACACTTTG
TTCCAAGATGAAAGTTATAAGAAGCACTTGAAACATCAGTGTAACAAGGTACTGTTGCGG
GTACGAATGCTATACTACCTGAGGCAGGAGGTTATTGGAGACCAAGCAGAAAAGGTGTTA
GGGGGTGCGATTGCCAGTGAGATTGACATATGGTTCCCAGTAGTGGATCAACTGGAGGTT
CCAACAACTTGGTGGGACAGTGAGGCTGACAAGTCGCTGCTCATTGGAGTCTTTAAACAT
GGCTATGAGAAATATAATACCATGAGGGCAGACCCAGCCTTATGTTTCCTAGAAAAGGCT
GGCCGACCAGATGACAAAGCAATTGCAGCAGAACATCGAGTGTTGGATAACTTCTCTGAC
ATAGTAGAAGGGGTTGACTTTGATAAAGATTGTGAAGATCCTGAATATAAACCACTCCAA
GGTCCCCCAAAGGACCAAGATGATGAGGGTGATCCCTTGATGATGATGGATGAGGAGATC
TCAGTGATTGATGGAGATGAAGCCCAGGTGACCCAACAGCCAGGCCATTTATTCTGGCCT
CCGGGCTCTGCCCTAACAGCTAGGCTTCGGCGTCTAGTAACAGCGTATCAGCGCAGCTAC
AAGAGAGAACAAATGAAGATAGAGGCTGCAGAACGTGGGGACCGGCGAAGGCGGCGTTGT
GAAGCAGCCTTCAAGCTGAAAGAAATTGCACGGCGGGAGAAACAACAACGATGGACAAGG
CGTGAACAAACTGATTTTTATCGAGTGGTGTCTACGTTTGGTGTGGAATATGACCCTGAC
ACCATGCAGTTCCATTGGGATCGCTTCCGCACTTTTGCTCGACTAGACAAAAAGACAGAT
GAAAGCCTTACCAAGTACTTCCATGGCTTTGTGGCCATGTGCCGCCAAGTATGCCGCCTT
CCCCCAGCAGCTGGAGATGAACCCCCCGACCCTAACCTGTTCATTGAGCCCATCACTGAG
GAGAGAGCCTCACGGACTCTCTACCGTATAGAATTGCTTCGGCGCTTACGGGAACAAGTT
TTATGCCACCCCCTTTTGGAAGATCGGCTGGCATTGTGTCAGCCTCCTGGTCCTGAATTG
CCCAAATGGTGGGAGCCTGTTCGGCATGATGGGGAGCTTCTAAGAGGGGCAGCCCGCCAT
GGGGTGAGCCAAACAGACTGCAACATCATGCAGGACCCAGACTTCTCTTTTCTGGCTGCC
CGTATGAATTATATGCAGAACCATCAAGCAGGAGCACCAGCTCCATCCTTGTCACGCTGC
TCTACTCCACTGCTGCACCAGCAGTATACCTCACGCACTGCCTCACCACTGCCCCTGCGC
CCAGATGCTCCTGTTGAAAAGTCACCCGAGGAGACAGCTACCCAGGTCCCCAGTCTGGAG
AGTCTGACTTTAAAGCTAGAGCACGAGGTGGTGGCCAGGAGCCGACCAACCCCACAAGAC
TATGAGATGCGAGTATCCCCCTCTGATACTACCCCTCTGGTTTCCCGGAGTGTTCCACCA
GTCAAACTGGAGGATGAGGATGATTCGGACTCTGAGCTGGACTTGAGCAAGCTGTCACCA
TCTTCTTCTTCTTCCTCATCCTCATCCAGCTCCAGCTCCAGCACTGATGAGAGTGAGGAT
GAGAAGGAAGAGAAGCTAACTGACCAGTCCCGCTCAAAGCTCTATGATGAAGAGAGTCTC
CTGTCCCTCACTATGTCCCAAGATGGATTCCCAAATGAAGATGGAGAACAAATGACCCCT
GAGCTTCTGCTACTGCAGGAAAGACAAAGAGCCTCTGAGTGGCCCAAGGATCGTGTCCTG
ATAAACCGTATTGACCTCGTCTGCCAGGCTGTACTCTCAGGGAAGTGGCCTTCTAGCCGT
AGGAGCCAGGAAATGGTAACAGGAGGAATTTTGGGGCCAGGCAACCACTTGCTAGACAGT
CCCTCATTGACTCCTGGAGAATATGGTGACTCTCCAGTCCCCACACCACGAAGTAGTAGT
GCAGCTTCCATGGCAGAGGAGGAAGCATCTGCAGTCAGCACAGCGGCAGCCCAGTTCACC
AAACTTCGCCGAGGCATGGATGAAAAGGAGTTTACAGTTCAAATCAAAGATGAGGAAGGA
TTGAAGTTAACATTCCAGAAGCACAAGTTGATGGCGAATGGAGTAATGGGAGATGGACAT
CCACTGTTTCATAAGAAGAAGGGGAACAGAAAGAAGCTAGTAGAGCTGGAGGTGGAGTGC
ATGGAAGAGCCTAATCACCTTGATGTGGACCTGGAGACCCGGATCCCTGTCATCAATAAG
GTGGATGGTACTTTGCTGGTGGGTGAGGATGCCCCTCGCCGGGCTGAACTGGAGATGTGG
TTACAGGGTCATCCAGAGTTTGCTGTTGATCCCCGATTTCTAGCGTATATGGAGGATCGC
AGAAAACAGAAGTGGCAAAGATGTAAAAAAAATAATAAGGCAGAATTGAACTGTTTGGGA
ATGGAACCAGTACAGACAGCTAACTCTAGAAATGGGAAAAAGGGTCATCACACTGAAACG
GTGTTCAACCGGGTTTTGCCAGGGCCTATTGCACCAGAGAGCAGCAAGAAGCGGGCCCGT
AGGATGCGACCAGACCTTTCTAAGATGATGGCCCTCATGCAGGGTGGAAGCACTGGGTCT
CTATCTCTGCATAACACGTTCCAACACAGCAGTAGTGGCCTACAGTCTGTGTCATCTTTG
GGTCACAGCAGTGCCACTTCTGCATCTTTGCCTTTTATGCCATTTGTGATGGGTGGTGCA
CCATCATCCCCTCATGTAGACTCCAGCACCATGCTTCATCACCACCACCACCACCCCCAC
CCCCACCATCACCACCATCACCATCCAGGCTTGAGAGCCCCTGGCTACCCCTCTTCACCA
GTGACTACCGCCTCTGGTACTACCTTGCGGTTGCCACCACTGCAACCTGAGGAGGATGAC
GATGAGGATGAAGAAGATGATGATGACTTATCTCAGGGCTATGATAGCTCAGAAAGGGAC
TTCTCACTCATTGATGATCCTATGATGCCAGCTAACTCAGACTCCAGTGAAGATGCTGAT
GACTTG
Protein sequence predicted based on nucleotide sequence
MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGN SSASELVPPPEETAPTELSKESTAPAPESITLHDYTTQPASQEQPAQPVLQTSTPTSGLL QVSKSQEILSQGNPFMGVSATAVSSSSAGGQPPQSAPKIVILKAPPSSSVTGAHVAQIQA QGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLAAKVPGNQAAVQRIVQ PSRPVKQLVLQPVKGSAPAGNPGATGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQ GHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSS QPQPQQPPSTQPVTLSSVQQAQIMGPGQSPGQRLSVPVKVVLQPQAGSSQGASSGLSVVK VLSASEVAALSSPASSAPHSGGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNI PRVLNEDELPSVRPEEEGEKKRRKKSAGERLKEEKPKKSKTSGASKTKGKSKLNTITPVV GKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEE EVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRIVKKELPSG QYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFN PDYVEVDRILDESHSIDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQS RHPELKRVNRPQASAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGL GKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQ QYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKL LDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQK LQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTN MPNLLNTMMELRKCCNHPYLINGAEEKILTEFREACHIIPHDFHLQAMVRSAGKLVLIDK LLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDS DRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITR NSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDD EGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKK ADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHA YGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENI KGFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTL FQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEV PTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSD IVEGVDFDKDCEDPEYKPLQGPPKDQDDEGDPLMMMDEEISVIDGDEAQVTQQPGHLFWP PGSALTARLRRLVTAYQRSYKREQMKIEAAERGDRRRRRCEAAFKLKEIARREKQQRWTR REQTDFYRVVSTFGVEYDPDTMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRL PPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGPEL PKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGAPAPSLSRC STPLLHQQYTSRTASPLPLRPDAPVEKSPEETATQVPSLESLTLKLEHEVVARSRPTPQD YEMRVSPSDTTPLVSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESED EKEEKLTDQSRSKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVL INRIDLVCQAVLSGKWPSSRRSQEMVTGGILGPGNHLLDSPSLTPGEYGDSPVPTPRSSS AASMAEEEASAVSTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHKLMANGVMGDGH PLFHKKKGNRKKLVELEVECMEEPNHLDVDLETRIPVINKVDGTLLVGEDAPRRAELEMW LQGHPEFAVDPRFLAYMEDRRKQKWQRCKKNNKAELNCLGMEPVQTANSRNGKKGHHTET VFNRVLPGPIAPESSKKRARRMRPDLSKMMALMQGGSTGSLSLHNTFQHSSSGLQSVSSL GHSSATSASLPFMPFVMGGAPSSPHVDSSTMLHHHHHHPHPHHHHHHHPGLRAPGYPSSP VTTASGTTLRLPPLQPEEDDDEDEEDDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDAD DL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
