Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868778
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5061nts
Open reading frame: 1 to 5061
ATGCCCGGCGGCCCCAGTCCCCGCAGCCCCGCGCCTTTGCTGCGCCCCCTCCTCCTGCTC
CTCTGCGCTCTGGCTCCCGGCGCCCCCGGACCCGCACCAGGACGTGCAACCGAGGGCCGG
GCGGCACTGGACATCGTGCACCCGGTTCGAGTCGACGCGGGGGGCTCCTTCCTGTCCTAC
GAGCTGTGGCCCCGCGCACTGCGCAAGCGGGATGTATCTGTGCGCCGAGACGCGCCCGCC
TTCTACGAGCTACAATACCGCGGGCGCGAGCTGCGCTTCAACCTGACCGCCAATCAGCAC
CTGCTGGCGCCCGGCTTTGTGAGCGAGACGCGGCGGCGCGGCGGCCTGGGCCGCGCGCAC
ATCCGGGCCCACACCCCGGCCTGCCACCTGCTTGGCGAGGTGCAGGACCCTGAGCTCGAG
GGTGGCCTGGCGGCCATCAGCGCCTGCGACGGCCTGAAAGGTGTGTTCCAGCTCTCCAAC
GAGGACTACTTCATTGAGCCCCTGGACAGTGCCCCGGCCCGGCCTGGCCACGCCCAGCCC
CATGTGGTGTACAAGCGTCAGGCCCCGGAGAGGCTGGCACAGCGGGGTGATTCCAGTGCT
CCAAGCACCTGTGGAGTGCAAGTGTACCCAGAGCTGGAGTCTCGACGGGAGCGTTGGGAG
CAGCGGCAGCAGTGGCGGCGGCCACGGCTGAGGCGTCTACACCAGCGGTCGGTCAGCAAA
GAGAAGTGGGTGGAGACCCTGGTAGTAGCTGATGCCAAAATGGTGGAGTACCACGGACAG
CCGCAGGTTGAGAGCTATGTGCTGACCATCATGAACATGGTGGCTGGCCTGTTTCATGAC
CCCAGCATTGGGAACCCCATCCACATCACCATTGTGCGCCTGGTCCTGCTGGAAGATGAG
GAGGAGGACCTAAAGATCACGCACCATGCAGACAACACCCTGAAGAGCTTCTGCAAGTGG
CAGAAAAGCATCAACATGAAGGGGGATGCCCATCCCCTGCACCATGACACTGCCATCCTG
CTCACCAGAAAGGACCTGTGTGCAGCCATGAACCGGCCCTGTGAGACCCTGGGACTGTCC
CATGTGGCGGGCATGTGCCAGCCGCACCGCAGCTGCAGCATCAACGAGGACACGGGCCTG
CCGCTGGCCTTCACTGTAGCCCACGAGCTCGGGCACAGTTTTGGCATTCAGCATGACGGA
AGCGGCAATGACTGTGAGCCCGTTGGGAAACGACCTTTCATCATGTCTCCACAGCTCCTG
TACGACGCCGCTCCCCTCACCTGGTCCCGCTGCAGCCGCCAGTATATCACCAGGTTCCTT
GACCGTGGGTGGGGCCTGTGCCTGGACGACCCTCCTGCCAAGGACATTATCGACTTCCCC
TCGGTGCCACCTGGCGTCCTCTATGATGTAAGCCACCAGTGCCGCCTCCAGTACGGGGCC
TACTCTGCCTTCTGCGAGGACATGGATAATGTCTGCCACACACTCTGGTGCTCTGTGGGG
ACCACCTGTCACTCCAAGCTGGATGCAGCTGTGGACGGCACCCGGTGTGGGGAGAATAAG
TGGTGTCTCAGTGGGGAGTGCGTACCCGTGGGCTTCCGGCCCGAGGCCGTGGATGGTGGC
TGGTCTGGCTGGAGCGCCTGGTCCATCTGCTCACGGAGCTGTGGCATGGGCGTACAGAGC
GCCGAGCGGCAGTGCACGCAGCCTACGCCCAAATACAAAGGCAGATACTGTGTGGGTGAG
CGCAAGCGCTTCCGCCTCTGCAACCTGCAGGCCTGCCCTGCTGGCCGCCCCTCCTTCCGC
CACGTCCAGTGCAGCCACTTTGACGCTATGCTCTACAAGGGCCAGCTGCACACATGGGTG
CCCGTGGTCAATGACGTGAACCCCTGCGAGCTGCACTGCCGGCCCGCGAATGAGTACTTT
GCCGAGAAGCTGCGGGACGCCGTGGTCGATGGCACCCCCTGCTACCAGGTCCGAGCCAGC
CGGGACCTCTGCATCAACGGCATCTGTAAGAACGTGGGCTGTGACTTCGAGATTGACTCC
GGTGCTATGGAGGACCGCTGTGGTGTGTGCCACGGCAACGGCTCCACCTGCCACACCGTG
AGCGGGACCTTCGAGGAGGCCGAGGGCCTGGGGTATGTGGATGTGGGGCTGATCCCAGCG
GGCGCACGCGAGATCCGCATCCAAGAGGTTGCCGAGGCTGCCAACTTCCTGGCACTGCGG
AGCGAGGACCCGGAGAAGTACTTCCTCAATGGTGGCTGGACCATCCAGTGGAACGGGGAC
TACCAGGTGGCAGGGACCACCTTCACATACGCACGCAGGGGCAACTGGGAGAACCTCACG
TCCCCGGGTCCCACCAAGGAGCCTGTCTGGATCCAGCTGCTGTTCCAGGAGAGCAACCCT
GGGGTGCACTACGAGTACACCATCCACAGGGAGGCAGGTGGCCACGACGAGGTCCCGCCG
CCCGTGTTCTCCTGGCATTATGGGCCCTGGACCAAGTGCACAGTCACCTGCGGCAGAGGT
GTGCAGAGGCAGAATGTGTACTGCTTGGAGCGGCAGGCAGGGCCCGTGGACGAGGAGCAC
TGTGACCCCCTGGGCCGGCCTGATGACCAACAGAGGAAGTGCAGCGAGCAGCCCTGCCCT
GCCAGGTGGTGGGCAGGTGAGTGGCAGCTGTGCTCCAGCTCCTGCGGGCCTGGGGGCCTC
TCCCGCCGGGCCGTGCTCTGCATCCGCAGCGTGGGGCTGGATGAGCAGAGCGCCCTGGAG
CCACCCGCCTGTGAACACCTTCCCCGGCCCCCTACTGAAACCCCTTGCAACCGCCATGTA
CCCTGTCCGGCCACCTGGGCTGTGGGGAACTGGTCTCAGTGCTCAGTGACATGTGGGGAG
GGCACTCAGCGCCGAAATGTCCTCTGCACCAATGACACCGGTGTCCCCTGTGACGAGGCC
CAGCAGCCAGCCAGCGAAGTCACCTGCTCTCTGCCACTCTGTCGGTGGCCCCTGGGCACA
CTGGGCCCTGAAGGCTCAGGCAGCGGCTCCTCCAGCCACGAGCTCTTCAACGAGGCTGAC
TTCATCCCGCACCACCTGGCCCCACGCCCTTCACCCGCCTCATCACCCAAGCCAGGCACC
ATGGGCAACGCCATTGAGGAGGAGGCTCCAGAGCTGGACCTGCCGGGGCCCGTGTTTGTG
GACGACTTCTACTACGACTACAATTTCATCAATTTCCACGAGGATCTGTCCTACGGGCCC
TCTGAGGAGCCCGATCTAGACCTGGCGGGGACAGGGGACCGGACACCCCCACCACACAGC
CATCCTGCTGCGCCCTCCACGGGTAGCCCCGTGCCTGCCACAGAGCCTCCTGCAGCCAAG
GAGGAGGGGGTACTGGGACCTTGGTCCCCGAGCCCTTGGCCTAGCCAGGCCGGCCGCTCC
CCACCCCCACCCTCAGAGCAGACCCCTGGGAACCCTTTGATCAATTTCCTGCCTGAGGAA
GACACCCCCATAGGGGCCCCAGATCTTGGGCTCCCCAGCCTGTCCTGGCCCAGGGTTTCC
ACTGATGGCCTGCAGACACCTGCCACCCCTGAGAGCCAAAATGATTTCCCAGTTGGCAAG
GACAGCCAGAGCCAGCTGCCCCCTCCATGGCGGGACAGGACCAATGAGGTTTTCAAGGAT
GATGAGGAACCCAAGGGCCGAGGAGCACCCCACCTGCCCCCGAGACCCAGCTCCACGCTG
CCCCCTTTGTCCCCTGTTGGCAGCACCCACTCCTCTCCTAGTCCTGACGTGGCGGAGCTG
TGGACAGGAGGCACAGTGGCCTGGGAGCCAGCTCTGGAGGGTGGCCTGGGGCCTGTGGAC
AGTGAACTGTGGCCCACTGTTGGGGTGGCTTCTCTCCTTCCTCCTCCCATAGCCCCTCTG
CCAGAGATGAAGGTCAGGGACAGTTCCCTGGAGCCGGGGACTCCCTCCTTCCCAACCCCA
GGACCAGGCTCATGGGACCTGCAGACTGTGGCAGTGTGGGGGACCTTCCTCCCCACAACC
CTGACTGGCCTCGGGCACATGCCTGAGCCTGCCCTGAACCCAGGACCCAAGGGTCAGCCT
GAGTCCCTCAGCCCTGAGGTGCCCCTGAGCTCTAGGCTGCTGTCCACACCAGCTTGGGAC
AGCCCCGCCAACAGCCACAGAGTCCCTGAGACCCAGCCGCTGGCTCCCAGCCTGGCTGAA
GCGGGGCCCCCCGCGGACCCGTTGGTTGTCAGGAACGCCGGCTGGCAAGCGGGAAACTGG
AGCGAGTGCTCTACCACCTGTGGCCTGGGTGCGGTCTGGAGGCCGGTGCGCTGTAGCTCC
GGCCGGGATGAGGACTGCGCCCCCGCTGGCCGGCCCCAGCCTGCCCGCCGCTGCCACCTG
CGGCCCTGTGCCACCTGGCACTCAGGCAACTGGAGTAAGTGCTCCCGCAGCTGCGGCGGA
GGTTCCTCAGTGCGGGACGTGCAGTGTGTGGACACACGGGACCTCCGGCCACTGCGGCCC
TTCCATTGTCAGCCCGGGCCTGCCAAGCCGCCTGCGCACCGGCCCTGCGGGGCCCAGCCC
TGCCTCAGCTGGTACACATCTTCCTGGAGGGAGTGCTCCGAGGCCTGTGGCGGTGGTGAG
CAGCAGCGTCTAGTGACCTGCCCGGAGCCAGGCCTCTGCGAGGAGGCGCTGAGACCCAAC
ACCACCCGGCCCTGCAACACCCACCCCTGCACGCAGTGGGTGGTGGGGCCCTGGGGCCAG
TGCTCAGGCCCCTGTGGTGGTGGTGTCCAGCGGCGCCTGGTCAAGTGTGTCAACACCCAG
ACAGGGCTGCCCGAGGAAGACAGTGACCAGTGTGGCCACGAGGCCTGGCCTGAGAGCTCC
CGGCCGTGTGGCACCGAGGATTGTGAGCCCGTCGAGCCTCCCCGCTGTGAGCGGGACCGC
CTGTCCTTCGGGTTCTGCGAGACGCTGCGCCTACTGGGCCGCTGCCAGCTGCCCACCATC
CGCACCCAGTGCTGCCGCTCGTGCTCTCCGCCCAGCCACGGCGCCCCCTCCCGAGGCCAT
CAGCGGGTTGCCCGCCGCTTG
Protein sequence predicted based on nucleotide sequence
MPGGPSPRSPAPLLRPLLLLLCALAPGAPGPAPGRATEGRAALDIVHPVRVDAGGSFLSY ELWPRALRKRDVSVRRDAPAFYELQYRGRELRFNLTANQHLLAPGFVSETRRRGGLGRAH IRAHTPACHLLGEVQDPELEGGLAAISACDGLKGVFQLSNEDYFIEPLDSAPARPGHAQP HVVYKRQAPERLAQRGDSSAPSTCGVQVYPELESRRERWEQRQQWRRPRLRRLHQRSVSK EKWVETLVVADAKMVEYHGQPQVESYVLTIMNMVAGLFHDPSIGNPIHITIVRLVLLEDE EEDLKITHHADNTLKSFCKWQKSINMKGDAHPLHHDTAILLTRKDLCAAMNRPCETLGLS HVAGMCQPHRSCSINEDTGLPLAFTVAHELGHSFGIQHDGSGNDCEPVGKRPFIMSPQLL YDAAPLTWSRCSRQYITRFLDRGWGLCLDDPPAKDIIDFPSVPPGVLYDVSHQCRLQYGA YSAFCEDMDNVCHTLWCSVGTTCHSKLDAAVDGTRCGENKWCLSGECVPVGFRPEAVDGG WSGWSAWSICSRSCGMGVQSAERQCTQPTPKYKGRYCVGERKRFRLCNLQACPAGRPSFR HVQCSHFDAMLYKGQLHTWVPVVNDVNPCELHCRPANEYFAEKLRDAVVDGTPCYQVRAS RDLCINGICKNVGCDFEIDSGAMEDRCGVCHGNGSTCHTVSGTFEEAEGLGYVDVGLIPA GAREIRIQEVAEAANFLALRSEDPEKYFLNGGWTIQWNGDYQVAGTTFTYARRGNWENLT SPGPTKEPVWIQLLFQESNPGVHYEYTIHREAGGHDEVPPPVFSWHYGPWTKCTVTCGRG VQRQNVYCLERQAGPVDEEHCDPLGRPDDQQRKCSEQPCPARWWAGEWQLCSSSCGPGGL SRRAVLCIRSVGLDEQSALEPPACEHLPRPPTETPCNRHVPCPATWAVGNWSQCSVTCGE GTQRRNVLCTNDTGVPCDEAQQPASEVTCSLPLCRWPLGTLGPEGSGSGSSSHELFNEAD FIPHHLAPRPSPASSPKPGTMGNAIEEEAPELDLPGPVFVDDFYYDYNFINFHEDLSYGP SEEPDLDLAGTGDRTPPPHSHPAAPSTGSPVPATEPPAAKEEGVLGPWSPSPWPSQAGRS PPPPSEQTPGNPLINFLPEEDTPIGAPDLGLPSLSWPRVSTDGLQTPATPESQNDFPVGK DSQSQLPPPWRDRTNEVFKDDEEPKGRGAPHLPPRPSSTLPPLSPVGSTHSSPSPDVAEL WTGGTVAWEPALEGGLGPVDSELWPTVGVASLLPPPIAPLPEMKVRDSSLEPGTPSFPTP GPGSWDLQTVAVWGTFLPTTLTGLGHMPEPALNPGPKGQPESLSPEVPLSSRLLSTPAWD SPANSHRVPETQPLAPSLAEAGPPADPLVVRNAGWQAGNWSECSTTCGLGAVWRPVRCSS GRDEDCAPAGRPQPARRCHLRPCATWHSGNWSKCSRSCGGGSSVRDVQCVDTRDLRPLRP FHCQPGPAKPPAHRPCGAQPCLSWYTSSWRECSEACGGGEQQRLVTCPEPGLCEEALRPN TTRPCNTHPCTQWVVGPWGQCSGPCGGGVQRRLVKCVNTQTGLPEEDSDQCGHEAWPESS RPCGTEDCEPVEPPRCERDRLSFGFCETLRLLGRCQLPTIRTQCCRSCSPPSHGAPSRGH QRVARRL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
