Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868779
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3864nts
Open reading frame: 1 to 3864
ATGCATCCGGATCTTGGACCCTTATGTACACTGCTGTATGTTACTCTTACAATTCTGTGC
TCTTCTGTGAGTTCAGACTTGGCACCTTATTTTACTTCTGAGCCGCTCTCTGCTGTCCAG
AAACTTGGTGGACCTGTAGTACTGCATTGTTCTGCTCAACCTGTGACCACTCGTATCTCA
TGGCTGCATAACGGAAAAACATTGGATGGAAACCTGGAACATGTTAAGATTCATCAGGGG
ACTCTGACAATTCTTTCTCTCAACTCCTCTCTTTTGGGTTACTACCAGTGCCTTGCCAAC
AATAGCATCGGTGCCATTGTGAGTGGCCCTGCGACAGTATCTGTGGCAGTTCTTGGTGAT
TTTGGTTCATCCACAAAGCATGTTATTACAGCAGAAGAAAAAAGTGCTGGTTTCATTGGC
TGCAGGGTACCGGAGAGTAACCCCAAAGCTGAGGTGCGCTATAAAATCCGGGGAAAATGG
CTGGAACATTCCACAGAGAATTACTTAATCCTTCCATCAGGAAATCTTCAGATTTTGAAT
GTATCCTTAGAGGACAAGGGATCATACAAATGTGCAGCTTATAATCCTGTCACACATCAA
TTAAAAGTTGAACCTATTGGCCGAAAGCTCCTTGTGAGTCGTCCTTCTTCAGATGATGTT
CACATTCTTCACCCCACCCATTCACAGGCATTAGCTGTTCTTTCTCGTAGCCCTGTAACC
TTGGAGTGTGTGGTGAGTGGGGTCCCGGCTCCTCAAGTGTATTGGCTAAAGGACGGGCAG
GACATTGCACCAGGAAGCAACTGGAGAAGGTTGTATTCTCATCTTGCCACTGATAGCGTT
GACCCGGCGGACTCCGGAAACTATTCCTGCATGGCGGGAAACAAGTCTGGAGATGTAAAA
TATGTGACTTACATGGTTAATGTACTTGAACATGCTTCCATTTCTAAAGGACTACAGGAT
CAGATAGTGTCTCTGGGTGCCACAGTACACTTTACCTGCGACGTTCATGGGAACCCAGCC
CCCAACTGTACCTGGTTTCACAATGCACAGCCTATTCATCCTTCTGCACGACATCTAACT
GCAGGAAACGGACTGAAAATCAGTGGGGTTACTGTGGAAGATGTTGGGATGTATCAGTGT
GTAGCAGATAATGGGATTGGATTTATGCACTCTACTGGAAGACTTGAAATTGAAAATGAC
GGTGGATTCAAGCCAGTTATAATTACGGCACCAGTAAGTGCAAAGGTTGCAGACGGAGAC
TTTGTTACTCTGTCCTGCAATGCCAGTGGGCTGCCGGTTCCGGTCATTCGTTGGTATGAC
AGCCATGGATTGATAACCAGCCATCCATCTCAAGTCCTGAGATCGAAATCCCGAAAATCA
CAGTTATCAAGACCTGAGGGCTTGAACCTGGAGCCTGTGTACTTCGTCCTGTCCCAAGCT
GGTGCAAGCTCTCTCCATATTCAGGCTGTGACTCAGGAACATGCGGGGAAATACATCTGC
GAAGCTGCAAATGAACATGGTACCACACAGGCAGAAGCATCTCTCATGGTTGTTCCTTTT
GAAACAAATACAAAAGCAGAGACAGTCACACTTCCTGATGCTGCTCAGAATGATGACAGA
AGTAAGAGAGATGGTTCAGAAACTGGGTTACTGAGCTCATTTCCGGTGAAGGTCCATCCC
AGTGCAGTGGAATCAGCACCAGAGAAAAACGCCAGCGGCATCTCTGTTCCTGATGCCCCC
ATCATACTGAGCCCCCCACAGACCCACACACCAGACACGTACAACCTGGTGTGGAGGGCA
GGCAAGGATGGTGGGCTGCCCATCAATGCTTACTTTGTGAAGTATCGAAAGCTGGATGAT
GGGGTTGGCATGCTGGGAAGCTGGCACACGGTTCGAGTCCCAGGAAGTGAAAATGAGCTC
CATTTAGCTGAGCTGGAGCCATCTAGTCTTTATGAAGTCTTGATGGTAGCAAGAAGCGCA
GCAGGTGAAGGCCAACCTGCCATGCTTACCTTCCGAACCAGCAAAGAAAAAACAGCGTCA
TCAAAAAACACCCAGGCATCCTCTCCACCCGTGGGCATCCCTAAGTATCCCGTTGTTTCA
GAGGCTGCAAACAACAATTTTGGAGTGGTACTTACAGATTCCTCTAGGCACAGTGGAGTT
CCAGAGGCACCAGATCGGCCTACCATCTCCACTGCATCAGAGACATCAGTCTATGTCACT
TGGATTCCTCGGGCAAACGGGGGTTCTCCAATCACTGCCTTCAAAGTCGAATATAAACGG
ATGAGGACCAGCAATTGGCTGGTGGCAGCTGAAGACATCCCTCCTTCCAAACTTTCAGTG
GAAGTTCGTAGTTTAGAACCAGGTTCAACATACAAATTTAGGGTCATTGCCATCAACCAT
TATGGTGAGAGTTTTCGGAGTTCAGCATCTCGTCCTTATCAAGTGGTTGGGTTCCCCAAT
CGCTTTTCCAGCCGTCCAATAACTGGACCTCACATTGCATACACAGAGGCTGTCAGCGAT
ACTCAGATCATGCTAAAGTGGACGTACATTCCATCAAGTAACAATAACACTCCCATTCAA
GGATTTTATATCTATTACCGACCAACAGATAGTGACAATGACAGTGATTACAAGAGGGAT
GTTGTAGAAGGTTCAAAGCAGTGGCACATGATTGGCCACCTGCAGCCAGAAACCTCCTAT
GACATTAAAATGCAATGCTTCAATGAAGGAGGAGAAAGTGAATTTAGCAATGTGATGATC
TGCGAGACTAAAGTGAAACGTGTTCCTGGAGCTTCTGAATATCCTGTCAAAGACTTGAGT
ACCCCTCCAAATTCTTTGGGAAGTGGAGGAAATGTGGGGCCTGCAACCAGCCCTGCCAGA
AGCAGTGACATGTTATATCTGATCGTTGGCTGTGTGCTGGGCGTCATGGTCCTCATTCTG
ATGGTTTTCATTGCAATGTGCCTGTGGAAGAATCGCCAGCAGAATACCATACAAAAATAT
GACCCACCAGGATATCTCTACCAAGGATCAGATATGAACGGGCAGATGGTGGACTACACC
ACTCTCTCAGGAGCAAGTCAGATAAATGGAAATGTTCACGGAGGCTTCCTAACCAATGGC
GGTCTCAGCAGTGGCTATTCCCACCTTCACCATAAGGTCCCCAATGCAGTCAATGGAATT
GTGAATGGGAGCCTAAATGGAGGGCTTTACTCCGGGCACAGCAACTCTCTAACCAGGACA
CACGTGGATTTTGAACATCCTCATCATCTAGTGAATGGTGGTGGAATGTACACGGCCGTG
CCTCAGATTGACCCTCTGGAGTGTGTTAACTGCCGAAATTGTCGAAACAACAATAGGTGT
TTCACCAAAACCAACAGCACTTTCAGCAGCAGCCCTCCTCCTGTGGTCCCTGTGGTAGCA
CCTTATCCTCAGGATGGTTTGGAAATGAAGCCCCTCAGTCACGTGAAGGTGCCTGTATGC
CTGACTTCCGCAGTCCCTGATTGTGGCCAGTTGCCGGAGGAGAGCGTCAAGGACAATGTG
GAACCAGTCCCTACTCAGCGTACCTGCTGTCAGGACATTGTAAATGACGTCAGCTCTGAT
GGCTCAGAAGATCCAGCAGAGTTCAGCAGAGGTCAGGAAGGCATGATCAACCTTAGGATT
CCAGATCATCTTCAGTTAGCTAAGAGCTGTGTATGGGAAGGAGACAGCTGTGCCCATTCA
GAAACAGAGATCAACATTGTAAGTTGGAATGCTCTTATTTTGCCACCTGTCCCCGAGGGC
TGTGCTGAGAAGACAATGTGGTCTCCACCTGGCATTCCTTTAGACAGCCCGACAGAGGTC
CTTCAGCAGCCCCGGGAAACCTTG
Protein sequence predicted based on nucleotide sequence
MHPDLGPLCTLLYVTLTILCSSVSSDLAPYFTSEPLSAVQKLGGPVVLHCSAQPVTTRIS WLHNGKTLDGNLEHVKIHQGTLTILSLNSSLLGYYQCLANNSIGAIVSGPATVSVAVLGD FGSSTKHVITAEEKSAGFIGCRVPESNPKAEVRYKIRGKWLEHSTENYLILPSGNLQILN VSLEDKGSYKCAAYNPVTHQLKVEPIGRKLLVSRPSSDDVHILHPTHSQALAVLSRSPVT LECVVSGVPAPQVYWLKDGQDIAPGSNWRRLYSHLATDSVDPADSGNYSCMAGNKSGDVK YVTYMVNVLEHASISKGLQDQIVSLGATVHFTCDVHGNPAPNCTWFHNAQPIHPSARHLT AGNGLKISGVTVEDVGMYQCVADNGIGFMHSTGRLEIENDGGFKPVIITAPVSAKVADGD FVTLSCNASGLPVPVIRWYDSHGLITSHPSQVLRSKSRKSQLSRPEGLNLEPVYFVLSQA GASSLHIQAVTQEHAGKYICEAANEHGTTQAEASLMVVPFETNTKAETVTLPDAAQNDDR SKRDGSETGLLSSFPVKVHPSAVESAPEKNASGISVPDAPIILSPPQTHTPDTYNLVWRA GKDGGLPINAYFVKYRKLDDGVGMLGSWHTVRVPGSENELHLAELEPSSLYEVLMVARSA AGEGQPAMLTFRTSKEKTASSKNTQASSPPVGIPKYPVVSEAANNNFGVVLTDSSRHSGV PEAPDRPTISTASETSVYVTWIPRANGGSPITAFKVEYKRMRTSNWLVAAEDIPPSKLSV EVRSLEPGSTYKFRVIAINHYGESFRSSASRPYQVVGFPNRFSSRPITGPHIAYTEAVSD TQIMLKWTYIPSSNNNTPIQGFYIYYRPTDSDNDSDYKRDVVEGSKQWHMIGHLQPETSY DIKMQCFNEGGESEFSNVMICETKVKRVPGASEYPVKDLSTPPNSLGSGGNVGPATSPAR SSDMLYLIVGCVLGVMVLILMVFIAMCLWKNRQQNTIQKYDPPGYLYQGSDMNGQMVDYT TLSGASQINGNVHGGFLTNGGLSSGYSHLHHKVPNAVNGIVNGSLNGGLYSGHSNSLTRT HVDFEHPHHLVNGGGMYTAVPQIDPLECVNCRNCRNNNRCFTKTNSTFSSSPPPVVPVVA PYPQDGLEMKPLSHVKVPVCLTSAVPDCGQLPEESVKDNVEPVPTQRTCCQDIVNDVSSD GSEDPAEFSRGQEGMINLRIPDHLQLAKSCVWEGDSCAHSETEINIVSWNALILPPVPEG CAEKTMWSPPGIPLDSPTEVLQQPRETL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
