Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868813
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3075nts
Open reading frame: 1 to 3075
ATGGCCCAGGGTGCCATGCGCTTCTGCTCGGAAGGCGACTGTGCCATCTCCCCACCACGA
TGCCCACGCCGCTGGCTCCCCGAAGGCCCGGTGCCCCAGAGCCCCCCAGCCAGCATGTAT
GGCAGCACAGGCTCCTTGCTACGGCGAGTGGCAGGTCCAGGTCCTCGAGGCCGGGAACTG
GGACGTGTGACAGCACCCTGTACACCTCTGCGTGGCCCCCCCTCACCCCGTGTTGCTCCC
TCACCCTGGGCACCCTCTTCACCCACTGGGCAGCCCCCACCCGGGGCCCAGAGCTCTGTG
GTCATCTTCCGCTTTGTGGAGAAGGCCAGTGTGAGGCCACTGAATGGGCTACCTGCTCCA
GGGGGCTTGAGTCGGAGCTGGGATCTGGGTGGGGTTTCTCCTCCCAGGCCCACCCCAGCC
CTTGGGCCTGGCTCCAACCGGAAGTTACGGCTGGAAGCATCCACATCAGACCCACTCCCC
GCCAGAGGAGGCTCGGCCCTACCTGGCAGCCGGAACCTTGTACATGGGCCGCCAGCCCCA
CCCCAGGTTGGAGCAGATGGCCTTTACTCCTCTCTCCCCAATGGGCTGGGGGGCCCCCCT
GAGCGCCTGGCCACACTCTTCGGAGGACCTGCTGACACTGGATTCCTGAACCAGGGGGAT
ACCTGGTCCTCCCCCCGGGAAGTCTCCTCTCATGCCCAGAGAATCGCTAGAGCCAAATGG
GAATTCTTCTATGGCTCCTTGGACCCCCCCAGCTCAGGTGCTAAGCCCCCAGAGCAGGCC
CCCCCATCTCCACCTGGGGTGGGCTCAAGGCAGGGCTCTGGGGTGGCTGTGGGGCGAGCA
GCCAAGTACTCCGAGACAGACCTGGACACGGTGCCCCTGAGGTGCTACCGCGAGACTGAC
ATCGATGAGGTGCTGGCTGAGCGGGAGGAGGCCGACTCGGCCATCGAAAGTCAGCCCAGC
TCTGAGGGCCCACCAGGCACTGCCTACCCACCTGCCCCACGGCCCGGCCCACTCCCTGGC
CCTCATCCCAGCCTCGGCAGTGGCAATGAGGATGAGGACGACGATGAGGCAGGTGGGGAA
GAAGATGTGGACGACGAGGTGTTTGAGGCCTCTGAAGGGGCCCGGCCAGGGAGCCGGATG
CCTCTCAAGTCACCTGTGCCCTTTCTACCTGGGACGAGCCCCTCGGCTGATGGGCCTGAC
TCTTTCAGTTGTGTGTTCGAAGCCATCCTGGAGTCACACCGGGCCAAAGGCACCTCCTAT
ACCAGCCTCGCCTCGCTGGAGGCCTTGGCCTCACCTGGCCCAACCCAGAGCCCCTTCTTC
ACCTTTGAGCTGCCTCCCCAACCCCCTGCACCCCGGCCCGACCCACCAGCTCCCGCCCCA
CTTGCCCCTCTTGAACCGGATTCTGGTACCAGCTCTGCTGCTGATGGTCCTTGGACACAG
AGAGGGGAGGAGGAGGAGGCAGAGGCCAGAGCCAAGCTGGCCCCAGGGAGGGAGCCCCCT
AGTCCCTGCCACTCAGAGGACAGCCTTGGGCTGGGGGCAGCACCCCTTGGCAGCGAACCA
CCCCTGAGCCAGCTGGTGTCCGACTCAGACTCAGAGCTGGACAGCACAGAGCGGCTGGCC
CTGGGAAGCACAGACACCTTGTCCAATGGGCAGAAAGCGGACCTGGAGGCTGCGCAGCGC
CTGGCCAAGAGGCTGTACCGACTAGATGGCTTCAGGAAGGCCGATGTGGCCCGGCACCTG
GGCAAGAACAATGACTTCAGCAAACTGGTGGCTGGGGAGTACCTCAAGTTCTTTGTCTTC
ACGGGCATGACTCTGGACCAAGCTCTCAGGGTGTTTCTGAAGGAGCTGGCCTTAATGGGT
GAGACCCAGGAACGAGAGCGCGTGCTGGCCCACTTCTCCCAGCGATACTTCCAGTGCAAT
CCTGAAGCCCTGTCCTCAGAGGACGGCGCCCACACGCTGACCTGTGCGCTCATGCTGCTC
AACACGGATCTCCACGGCCATAACATCGGGAAGCGCATGACCTGCGGGGACTTCATCGGG
AACCTGGAGGGCCTCAATGATGGCGGCGACTTCCCTAGGGAGCTGCTCAAGGCCTTGTAC
AGCTCCATCAAGAATGAGAAGCTGCAGTGGGCCATAGACGAGGAGGAGCTGAGACGCTCT
CTGTCTGAGTTGGCCGACCCCAACCCCAAGGTCATCAAGCGGATCAGCGGGGGCAGTGGC
AGTGGCTCCAGCCCTTTCCTGGACCTGACTCCCGAGCCTGGGGCTGCCGTCTACAAGCAC
GGGGCCCTGGTGCGAAAGGTGCACGCAGACCCTGACTGCAGGAAGACACCTCGGGGCAAG
CGGGGCTGGAAGAGCTTCCACGGGATCCTCAAGGGCATGATCCTCTACCTGCAGAAGGAG
GAGTACAAGCCTGGGAAGGCCCTTTCAGAGACGGAGCTCAAGAATGCCATCAGCATCCAC
CATGCCCTGGCCACTCGTGCCAGTGACTACAGCAAGAGGCCCCACGTCTTCTACCTGCGC
ACAGCTGACTGGCGGGTCTTCCTCTTCCAGGCCCCGAGCCTGGAGCAGATGCAGTCCTGG
ATCACTCGCATCAATGTAGTAGCCGCTATGTTCTCTGCGCCCCCCTTCCCAGCTGCTGTT
AGCTCCCAAAAGAAGTTCAGCCGCCCTCTCCTGCCCAGCGCTGCCACCCGCCTCTCCCAG
GAGGAGCAGGTGCGGACCCACGAGGCCAAGCTGAAGGCCATGGCAAGTGAGCTGCGGGAG
CACCGGGCCGCCCAGCTGGGCAAGAAGGGCCGGGGCAAGGAGGCTGAAGAGCAGCGGCAG
AAGGAGGCCTACCTGGAGTTTGAGAAATCCCGCTACAGCACCTATGCAGCGCTGCTTCGG
GTCAAGCTGAAGGCAGGCAGTGAGGAGCTGGATGCAGTGGAGGCAGCACTGGCCCAGGCC
GGGAGCACAGAGGATGGACTCCCTCCTTCTCACTCCAGTCCCTCCCTGCAGCCCAAACCC
TCCAGCCAGCCCCGGGCTCAGCGTCACAGCTCAGAGCCTCGGCCAGGGGCAGGCAGTGGG
CGGCGGAAGCCCTTG
Protein sequence predicted based on nucleotide sequence
MAQGAMRFCSEGDCAISPPRCPRRWLPEGPVPQSPPASMYGSTGSLLRRVAGPGPRGREL GRVTAPCTPLRGPPSPRVAPSPWAPSSPTGQPPPGAQSSVVIFRFVEKASVRPLNGLPAP GGLSRSWDLGGVSPPRPTPALGPGSNRKLRLEASTSDPLPARGGSALPGSRNLVHGPPAP PQVGADGLYSSLPNGLGGPPERLATLFGGPADTGFLNQGDTWSSPREVSSHAQRIARAKW EFFYGSLDPPSSGAKPPEQAPPSPPGVGSRQGSGVAVGRAAKYSETDLDTVPLRCYRETD IDEVLAEREEADSAIESQPSSEGPPGTAYPPAPRPGPLPGPHPSLGSGNEDEDDDEAGGE EDVDDEVFEASEGARPGSRMPLKSPVPFLPGTSPSADGPDSFSCVFEAILESHRAKGTSY TSLASLEALASPGPTQSPFFTFELPPQPPAPRPDPPAPAPLAPLEPDSGTSSAADGPWTQ RGEEEEAEARAKLAPGREPPSPCHSEDSLGLGAAPLGSEPPLSQLVSDSDSELDSTERLA LGSTDTLSNGQKADLEAAQRLAKRLYRLDGFRKADVARHLGKNNDFSKLVAGEYLKFFVF TGMTLDQALRVFLKELALMGETQERERVLAHFSQRYFQCNPEALSSEDGAHTLTCALMLL NTDLHGHNIGKRMTCGDFIGNLEGLNDGGDFPRELLKALYSSIKNEKLQWAIDEEELRRS LSELADPNPKVIKRISGGSGSGSSPFLDLTPEPGAAVYKHGALVRKVHADPDCRKTPRGK RGWKSFHGILKGMILYLQKEEYKPGKALSETELKNAISIHHALATRASDYSKRPHVFYLR TADWRVFLFQAPSLEQMQSWITRINVVAAMFSAPPFPAAVSSQKKFSRPLLPSAATRLSQ EEQVRTHEAKLKAMASELREHRAAQLGKKGRGKEAEEQRQKEAYLEFEKSRYSTYAALLR VKLKAGSEELDAVEAALAQAGSTEDGLPPSHSSPSLQPKPSSQPRAQRHSSEPRPGAGSG RRKPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
