Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00869030
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4605nts
Open reading frame: 1 to 4605
ATGGCGCTGACTCCCGGGTGGGGGTCCTCGGCGGGGCCGGTCCGGCCGGAGCTCTGGCTG
CTGCTGTGGGCAGCCGCGTGGCGCCTGGGTGCCTCGGCGTGCCCCGCCCTCTGCACCTGC
ACCGGAACCACGGTGGACTGCCACGGCACGGGGCTGCAGGCCATTCCCAAGAATATACCT
CGGAACACCGAGCGCCTGGAACTCAATGGCAACAACATCACTCGGATCCATAAGAATGAC
TTTGCGGGGCTCAAGCAGCTGCGGGTGCTGCAGCTGATGGAGAACCAGATTGGAGCAGTG
GAACGTGGTGCTTTTGATGACATGAAGGAGCTGGAGCGGCTGCGACTGAACCGAAACCAG
CTGCACATGTTACCGGAACTGCTGTTCCAGAACAACCAGGCTTTGTCAAGACTGGACTTG
AGTGAGAACGCCATCCAGGCCATCCCCAGGAAAGCTTTTCGGGGAGCTACGGACCTTAAA
AATTTACAGCTGGACAAGAACCAGATCAGCTGCATTGAGGAAGGGGCCTTCCGTGCTCTG
CGGGGGCTGGAGGTGCTGACCCTGAACAACAACAATATCACCACCATCCCCGTGTCCAGC
TTCAACCATATGCCCAAGCTACGGACCTTCCGCCTGCACTCCAACCACCTGTTTTGCGAC
TGCCACCTGGCCTGGCTCTCGCAGTGGCTGAGGCAGCGGCCAACCATCGGGCTCTTCACC
CAGTGCTCGGGCCCAGCCAGCCTGCGTGGCCTCAATGTGGCAGAGGTCCAGAAGAGTGAG
TTCAGCTGCTCAGGCCAGGGAGAAGCGGGGCGCGTGCCCACCTGCACCCTGTCCTCCGGC
TCCTGCCCGGCCATGTGCACCTGCAGCAATGGCATCGTGGACTGTCGTGGAAAAGGCCTC
ACTGCCATCCCGGCCAACCTGCCCGAGACCATGACGGAGATACGCCTGGAGCTGAACGGC
ATCAAGTCCATCCCTCCTGGAGCCTTCTCACCCTACAGAAAGCTACGGAGGATAGACCTG
AGCAACAATCAGATCGCTGAGATTGCACCCGACGCCTTCCAGGGCCTCCGCTCCCTGAAC
TCGCTGGTCCTCTATGGAAACAAGATCACAGACCTCCCCCGTGGTGTGTTTGGAGGCCTA
TACACCCTACAGCTCCTGCTCCTGAATGCCAACAAGATCAACTGCATCCGGCCCGATGCC
TTCCAGGACCTGCAGAACCTCTCACTGCTCTCCCTGTATGACAACAAGATCCAGAGCCTC
GCCAAGGGCACTTTCACCTCCCTGCGGGCCATCCAGACTCTGCACCTGGCGCAGAACCCT
TTCATTTGCGACTGTAACCTCAAGTGGCTGGCAGACTTCCTGCGCACCAATCCCATCGAG
ACGAGTGGTGCCCGCTGTGCCAGTCCCCGGCGCCTCGCCAACAAGCGCATCGGGCAGATC
AAGAGCAAGAAGTTCCGGTGCTCAGCCAAAGAGCAGTACTTCATTCCAGGCACGGAGGAT
TACCAGCTGAACAGCGAGTGCAACAGCGACGTGGTCTGTCCCCACAAGTGCCGCTGTGAG
GCCAACGTGGTGGAGTGCTCCAGCCTGAAGCTCACCAAGATCCCTGAGCGCATCCCCCAG
TCCACGGCAGAACTGCGATTGAATAACAATGAGATTTCCATCCTGGAGGCCACTGGGATG
TTTAAAAAACTTACACATCTGAAGAAAATCAATCTGAGCAACAACAAGGTGTCAGAAATT
GAAGATGGGGCCTTCGAGGGCGCAGCCTCTGTGAGCGAGCTGCACCTAACTGCCAACCAG
CTGGAGTCCATCCGGAGCGGCATGTTCCGGGGTCTGGATGGCTTGAGGACCCTAATGCTG
CGGAACAACCGCATCAGCTGCATCCACAACGACAGCTTCACGGGCCTGCGCAACGTCCGG
CTCCTCTCGCTCTACGACAACCAGATCACCACCGTATCCCCAGGAGCCTTCGACACCCTC
CAGTCCCTCTCCACACTGAATCTCCTGGCCAACCCTTTCAACTGCAACTGCCAGCTGGCC
TGGCTAGGAGGCTGGCTACGGAAGCGCAAGATCGTGACGGGGAACCCGCGATGCCAGAAC
CCTGACTTTTTGCGGCAGATTCCCCTGCAGGACGTGGCCTTCCCTGACTTCAGGTGTGAG
GAAGGCCAGGAGGAGGGGGGCTGCCTGCCCCGCCCACAGTGCCCACAGGAGTGCGCCTGC
CTGGACACCGTGGTCCGATGCAGCAACAAGCACCTGCGGGCCCTGCCCAAGGGCATTCCC
AAGAATGTCACAGAACTCTATTTGGACGGGAACCAGTTCACGCTGGTTCCGGGACAGCTG
TCTACCTTCAAGTACCTGCAGCTCGTGGACCTGAGCAACAACAAGATCAGTTCCTTAAGC
AATTCCTCCTTCACCAACATGAGCCAGCTGACCACTCTGATCCTCAGCTACAATGCCCTG
CAGTGCATCCCGCCTTTGGCCTTCCAGGGACTCCGCTCCCTGCGCCTGCTGTCTCTCCAC
GGCAATGACATCTCCACCCTCCAAGAGGGCATCTTTGCAGACGTGACCTCCCTGTCTCAC
CTGGCCATTGGTGCCAACCCCCTATACTGTGACTGCCACCTCCGCTGGCTGTCCAGCTGG
GTGAAGACTGGCTACAAGGAACCGGGCATTGCTCGTTGTGCTGGGCCCCAGGACATGGAG
GGCAAGCTGCTCCTCACCACGCCTGCCAAGAAGTTTGAATGCCAAGGTCCTCCAACGCTG
GCTGTCCAGGCCAAGTGTGATCTCTGCTTGTCCAGTCCGTGCCAGAACCAGGGCACCTGC
CACAACGACCCCCTTGAGGTGTACAGGTGCGCCTGCCCCAGCGGCTATAAGGGTCGAGAC
TGTGAGGTGTCCCTGGACAGCTGTTCCAGTGGCCCCTGTGAAAATGGGGGCACCTGCCAT
GCACAGGAGGGCGAGGATGCCCCGTTCACGTGCTCCTGTCCCACCGGCTTTGAAGGACCA
ACCTGTGGGGTGAACACAGATGACTGTGTGGATCATGCCTGTGCCAATGGGGGCGTCTGT
GTGGATGGTGTGGGCAACTACACCTGCCAGTGCCCCCTGCAGTATGAGGGAAAGGCCTGT
GAGCAGCTGGTGGACTTGTGCTCTCCGGATCTGAACCCATGTCAACACGAGGCCCAGTGT
GTGGGCACCCCGGATGGGCCCAGGTGTGAGTGCATGCCAGGTTATGCAGGTGACAACTGC
AGTGAGAACCAGGATGACTGCAGGGACCACCGCTGCCAGAATGGGGCCCAGTGTATGGAT
GAAGTCAACAGCTACTCCTGCCTCTGTGCTGAGGGCTACAGTGGACAGCTCTGTGAGATC
CCTCCCCATCTGCCTGCCCCCAAGAGCCCCTGTGAGGGGACTGAGTGCCAGAATGGGGCC
AACTGTGTGGACCAGGGCAACAGGCCTGTGTGCCAGTGCCTCCCAGGCTTCGGTGGCCCT
GAGTGTGAGAAGTTGCTCAGTGTCAACTTTGTGGATCGGGACACTTACCTGCAGTTCACT
GACCTGCAAAACTGGCCACGGGCCAACATCACGTTGCAGGTCTCCACGGCAGAGGACAAT
GGGATCCTTCTGTACAACGGGGACAACGACCACATTGCAGTTGAGCTGTACCAGGGCCAT
GTGCGTGTCAGCTACGACCCAGGCAGCTACCCCAGCTCTGCCATCTACAGTGCTGAGACG
ATCAACGATGGGCAATTCCACACCGTTGAGCTGGTTGCCTTTGACCAGATGGTGAATCTC
TCCATTGATGGCGGGAGCCCCATGACCATGGACAACTTTGGCAAACATTACACGCTCAAC
AGCGAGGCGCCACTCTATGTGGGAGGGATGCCCGTGGATGTCAACTCAGCTGCCTTCCGC
CTGTGGCAGATCCTCAACGGCACCGGCTTCCACGGTTGCATCCGAAACCTGTACATCAAC
AACGAGCTGCAGGACTTCACCAAGACGCAGATGAAGCCAGGCGTGGTGCCAGGCTGCGAA
CCCTGCCGCAAGCTCTACTGCCTGCATGGCATCTGCCAGCCCAATGCCACCCCAGGGCCC
ATGTGCCACTGCGAGGCTGGCTGGGTGGGCCTGCACTGTGACCAGCCCGCTGACGGCCCC
TGCCATGGCCACAAGTGTGTCCATGGGCAATGCGTGCCCCTCGACGCTCTTTCCTACAGC
TGCCAGTGCCAGGATGGGTACTCGGGGGCACTGTGCAACCAGGCCGGGGCCCTGGCAGAG
CCCTGCAGAGGCCTGCAGTGCCTGCATGGCCACTGCCAGGCCTCAGGCACCAAGGGGGCA
CACTGTGTGTGTGACCCCGGCTTTTCGGGCGAGCTGTGTGAGCAAGAGTCCGAGTGCCGG
GGGGACCCTGTCCGGGACTTTCACCAGGTCCAGAGGGGCTATGCCATCTGCCAGACCACG
CGCCCCCTGTCATGGGTGGAGTGCCGGGGCTCGTGCCCAGGCCAGGGCTGCTGCCAGGGC
CTTCGGCTGAAGCGGAGGAAGTTCACCTTTGAGTGCAGCGATGGGACCTCTTTTGCCGAG
GAGGTGGAAAAGCCCACCAAGTGTGGCTGTGCCCTCTGCGCATTG
Protein sequence predicted based on nucleotide sequence
MALTPGWGSSAGPVRPELWLLLWAAAWRLGASACPALCTCTGTTVDCHGTGLQAIPKNIP RNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQ LHMLPELLFQNNQALSRLDLSENAIQAIPRKAFRGATDLKNLQLDKNQISCIEEGAFRAL RGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFT QCSGPASLRGLNVAEVQKSEFSCSGQGEAGRVPTCTLSSGSCPAMCTCSNGIVDCRGKGL TAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLN SLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSL AKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETSGARCASPRRLANKRIGQI KSKKFRCSAKEQYFIPGTEDYQLNSECNSDVVCPHKCRCEANVVECSSLKLTKIPERIPQ STAELRLNNNEISILEATGMFKKLTHLKKINLSNNKVSEIEDGAFEGAASVSELHLTANQ LESIRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNQITTVSPGAFDTL QSLSTLNLLANPFNCNCQLAWLGGWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCE EGQEEGGCLPRPQCPQECACLDTVVRCSNKHLRALPKGIPKNVTELYLDGNQFTLVPGQL STFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLH GNDISTLQEGIFADVTSLSHLAIGANPLYCDCHLRWLSSWVKTGYKEPGIARCAGPQDME GKLLLTTPAKKFECQGPPTLAVQAKCDLCLSSPCQNQGTCHNDPLEVYRCACPSGYKGRD CEVSLDSCSSGPCENGGTCHAQEGEDAPFTCSCPTGFEGPTCGVNTDDCVDHACANGGVC VDGVGNYTCQCPLQYEGKACEQLVDLCSPDLNPCQHEAQCVGTPDGPRCECMPGYAGDNC SENQDDCRDHRCQNGAQCMDEVNSYSCLCAEGYSGQLCEIPPHLPAPKSPCEGTECQNGA NCVDQGNRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQVSTAEDN GILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVAFDQMVNL SIDGGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTGFHGCIRNLYIN NELQDFTKTQMKPGVVPGCEPCRKLYCLHGICQPNATPGPMCHCEAGWVGLHCDQPADGP CHGHKCVHGQCVPLDALSYSCQCQDGYSGALCNQAGALAEPCRGLQCLHGHCQASGTKGA HCVCDPGFSGELCEQESECRGDPVRDFHQVQRGYAICQTTRPLSWVECRGSCPGQGCCQG LRLKRRKFTFECSDGTSFAEEVEKPTKCGCALCAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
