Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00869046
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4026nts
Open reading frame: 1 to 4026
ATGAAACATCTGTATGGATTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACTT
CCAGATCCCACAGACACCTGGGAAATTATAGAGACCATTGGTAAAGGCACCTATGGCAAA
GTCTACAAGGTAACTAACAAGAGAGATGGGAGCCTGGCTGCAGTGAAAATTCTGGATCCA
GTCAGTGATATGGATGAAGAAATTGAGGCAGAATACAACATTTTGCAGTTCCTTCCTAAT
CATCCCAATGTTGTAAAGTTTTATGGGATGTTTTACAAAGCGGATCACTGTGTAGGGGGA
CAGCTGTGGCTGGTCCTGGAGCTGTGTAATGGGGGCTCAGTCACTGAGCTTGTCAAAGGT
CTACTCAGATGTGGCCAGCGGTTGGATGAAGCAATGATCTCATACATCTTGTACGGGGCC
CTCTTGGGCCTTCAGCATTTGCACAACAACCGAATCATCCACCGTGATGTGAAGGGGAAT
AACATTCTTCTGACAACAGAAGGAGGAGTTAAGCTCGTTGACTTTGGTGTTTCAGCTCAA
CTCACCAGTACACGTCTGCGGAGAAACACATCTGTTGGCACCCCGTTCTGGATGGCCCCT
GAGGTCATTGCCTGTGAGCAGCAGTATGACTCTTCCTATGACGCTCGCTGTGACGTCTGG
TCCTTGGGGATCACAGCTATTGAACTGGGGGATGGAGACCCTCCCCTCTTTGACATGCAT
CCTGTGAAAACACTCTTTAAGATTCCAAGAAATCCTCCACCTACTTTACTTCATCCAGAA
AAATGGTGTGAAGAATTCAACCACTTTATTTCACAGTGTCTTATTAAGGATTTTGAAAGG
CGACCTTCCGTCACACATCTCCTTGACCACCCATTTATTAAAGGAGTACATGGAAAAGTT
CTGTTTCTGCAAAAACAGCTGGCCAAGGTTCTCCAAGACCAGAAGCATCAAAATCCTGTT
GCTAAAACCAGGCATGAGAGGATGCATACCAGAAGACCTTATCATGTGGAAGATGCTGAA
AAATACTGCCTTGAGGATGATTTGGTCAACCTAGAGGTTCTGGATGAGGATACAATTATC
CATCAGTTGCAGAAACGTTATGCAGACTTGCTAATTTACACATATGTTGGAGACATCTTA
ATTGCCTTAAACCCCTTCCAGAATCTAAGCATATACTCTCCACAGTTTTCCAGACTTTAT
CATGGGGTGAAACGCGCCTCCAATCCCCCCCACATATTTGCATCAGCAGATGCTGCTTAC
CAGTGCATGGTTACTCTCAGCAAAGACCAGTGCATTGTCATCAGCGGAGAGAGTGGCTCT
GGGAAGACAGAAAGCGCCCACCTGATTGTTCAGCATTTGACTTTCTTGGGAAAGGCCAAT
AATCAGACCTTGAGAGAGAAAATTCTACAAGTCAACTCCCTGGTGGAAGCCTTTGGGAAC
TCATGCACTGCCATCAATGACAACTCGAGCCGTTTTGGAAAATATCTGGAAATGATGTTT
ACACCAACTGGAGTTGTGATGGGGGCAAGAATCTCTGAATATCTCCTGGAAAAATCCAGA
GTTATAAAACAGGCAGCGAGAGAGAAAAATTTTCATATATTTTACTATATTTATGCTGGT
CTTCATCACCAAAAGAAGCTTTCTGATTTCAGACTTCCTGAGGAAAAACCTCCTAGGTAC
ATAGCTGATGAAACTGGAAGGGTGATGCACGACATAACTTCCAAGGAGTCTTACAGAAGA
CAATTCGAAGCAATTCAGCATTGCTTCAGGATTATAGGGTTCACGGACAAAGAGGTGCAC
TCAGTGTACAGAATTTTGGCTGGGATTTTGAATATTGGGAACATTGAGTTCGCAGCTATT
TCCTCTCAACATCAGACTGATAAAAGTGAGGTGCCCAATGCTGAAGCTTTGCAAAATGCT
GCCTCTGTTCTGTGCATTAGCCCTGAAGAGCTCCAGGAGGCCCTCACCTCCCACTGTGTG
GTCACCCGGGGCGAGACCATCATCCGTGCCAACACTGTAGACAGGGCTGCGGACGTTCGA
GACGCCATGTCCAAAGCCCTGTATGGGAGGCTCTTCAGCTGGATTGTGAATCGCATTAAT
ACACTCCTGCAGCCAGACGAAAACATATGTAGTGCAGGAGGTGGAATGAATGTGGGGATC
TTGGATATCTTTGGATTCGAGAATTTTCAGAGAAATTCATTTGAGCAGCTCTGCATAAAC
ATCGCCAATGAGCAAATCCAGTACTATTTCAATCAGCATGTTTTTGCTCTTGAGCAGATG
GAATATCAGAATGAAGGCATTGATGCTGTACCCGTGGAATATGAGGACAACCGCCCGCTC
TTGGACATGTTCCTCCAGAAACCCCTGGGACTGCTTGCACTTTTGGATGAGGAAAGTCGG
TTTCCCCAAGCAACTGACCAGACCCTGGTTGATAAATTTGAAGATAATCTACGATGCAAA
TACTTCTGGAGGCCCAAAGGAGTGGAACTGTGCTTTGGCATTCAGCATTATGCTGGAAAG
GTATTATATGATGCTTCTGGGGTTCTTGAGAAAAATAGAGACACTCTCCCTGCCGATGTG
GTTGTGGTCCTGAGAACGTCAGAAAACAAGCTTCTTCAGCAGCTCTTCTCAATCCCTCTG
ACCAAAACAGGTAATTTGGCCCAGACAAGAGCTAGGATAACAGTGGCCTCAAGTTCTTTG
CCTCCACATTTCAGTGCTGGGAAAGCCAAGGTGGACACTCTGGAGGTGATACGGCATCCG
GAAGAAACCACCAACATGAAGAGGCAAACTGTGGCTTCTTACTTCCGGTATTCTCTGATG
GACCTGCTCTCCAAAATGGTGGTTGGACAGCCCCACTTTGTGCGCTGCATTAAACCCAAT
GATGACCGAGAGGCCCTGCAGTTCTCTCGAGAGAGGGTGCTGGCCCAGCTCCGCTCCACA
GGGATTCTGGAGACAGTCAGCATCCGCCGCCAGGGCTATTCCCACCGCATCCTTTTTGAA
GAATTTGTGAAAAGGTATTATTACTTGGCATTCACAGCACATCAAACACCTCTTGCTAGC
AAAGAGAGCTGTGTGGCTATCTTGGAAAAGTCCAGATTAGATCACTGGGTACTGGGAAAA
ACAAAGGTTTTTCTCAAATATTACCATGTTGAGCAATTAAATTTGCTGCTTCGAGAAGTC
ATAGGCAGAGTGGTTGTGCTGCAGGCATATACCAAGGGGTGGCTTGGAGCCAGGAGATAC
AAAAGGGTCAGAGAGAAGAGAGAGAAGGGAGCCATTGCCATCCAGTCAGCCTGGAGAGGA
TATGATGCTCGGAGGAAATTTAAGAAAATAAGCAACAGAAGGAATGAGTCTGCTGCTCAT
AATCAAGCAGGGGACACTTCAAACCAAAGCAGTGGGCCACATTCCCCCGTCGCAGCAGGT
ACGAGGGGAAGTGCCGAGGTTCAAGACTGCAGCGAGCCTGGTGACCATAAAGTTCTCAGG
GGCTCTGTACATCGTAGGAGCCATTCACAAGCAGAATCCAACAATGGCCGTACACAGACT
TCAAGCAACTCTCCTGCTGTCACAGAGAAAAATGGGCATTCACAAGCCCAGAGTTCTCCA
AAAGGGTGCGATATCTTCGCAGGACATGCAAACAAGCACTCGGTTTCTGGGACTGATTTG
CTGTCTTCTCGGATATGCCATCCTGCTCCAGATCAGCAAGGATTGAGTCTCTGGGGAGCC
CCTCAAAAGCCTGGTTCAGAAAATGGTCTTGCACAGAAGCATCGAACACCTCGCCGACGA
TGTCAGCAGCCCAAAATGCTGAGTAGCCCTGAGGACACCATGTACTATAACCAGTTAAAT
GGAACTCTAGAATATCAAGGGAGCAAGAGGAAGCCAAGAAAACTTGGCCAAATCAAAGTA
CTTGATGGGGAAGATGAATATTACAAATCTCTGTCACCAGTGGACTGTATCCCTGAGGAG
AACAACTCAGCCCACCCTTCCTTTTTTTCTTCATCCTCAAAAGGAGACTCTTTTGCTCAA
CATTTG
Protein sequence predicted based on nucleotide sequence
MKHLYGLFHYNPMMLGLESLPDPTDTWEIIETIGKGTYGKVYKVTNKRDGSLAAVKILDP VSDMDEEIEAEYNILQFLPNHPNVVKFYGMFYKADHCVGGQLWLVLELCNGGSVTELVKG LLRCGQRLDEAMISYILYGALLGLQHLHNNRIIHRDVKGNNILLTTEGGVKLVDFGVSAQ LTSTRLRRNTSVGTPFWMAPEVIACEQQYDSSYDARCDVWSLGITAIELGDGDPPLFDMH PVKTLFKIPRNPPPTLLHPEKWCEEFNHFISQCLIKDFERRPSVTHLLDHPFIKGVHGKV LFLQKQLAKVLQDQKHQNPVAKTRHERMHTRRPYHVEDAEKYCLEDDLVNLEVLDEDTII HQLQKRYADLLIYTYVGDILIALNPFQNLSIYSPQFSRLYHGVKRASNPPHIFASADAAY QCMVTLSKDQCIVISGESGSGKTESAHLIVQHLTFLGKANNQTLREKILQVNSLVEAFGN SCTAINDNSSRFGKYLEMMFTPTGVVMGARISEYLLEKSRVIKQAAREKNFHIFYYIYAG LHHQKKLSDFRLPEEKPPRYIADETGRVMHDITSKESYRRQFEAIQHCFRIIGFTDKEVH SVYRILAGILNIGNIEFAAISSQHQTDKSEVPNAEALQNAASVLCISPEELQEALTSHCV VTRGETIIRANTVDRAADVRDAMSKALYGRLFSWIVNRINTLLQPDENICSAGGGMNVGI LDIFGFENFQRNSFEQLCINIANEQIQYYFNQHVFALEQMEYQNEGIDAVPVEYEDNRPL LDMFLQKPLGLLALLDEESRFPQATDQTLVDKFEDNLRCKYFWRPKGVELCFGIQHYAGK VLYDASGVLEKNRDTLPADVVVVLRTSENKLLQQLFSIPLTKTGNLAQTRARITVASSSL PPHFSAGKAKVDTLEVIRHPEETTNMKRQTVASYFRYSLMDLLSKMVVGQPHFVRCIKPN DDREALQFSRERVLAQLRSTGILETVSIRRQGYSHRILFEEFVKRYYYLAFTAHQTPLAS KESCVAILEKSRLDHWVLGKTKVFLKYYHVEQLNLLLREVIGRVVVLQAYTKGWLGARRY KRVREKREKGAIAIQSAWRGYDARRKFKKISNRRNESAAHNQAGDTSNQSSGPHSPVAAG TRGSAEVQDCSEPGDHKVLRGSVHRRSHSQAESNNGRTQTSSNSPAVTEKNGHSQAQSSP KGCDIFAGHANKHSVSGTDLLSSRICHPAPDQQGLSLWGAPQKPGSENGLAQKHRTPRRR CQQPKMLSSPEDTMYYNQLNGTLEYQGSKRKPRKLGQIKVLDGEDEYYKSLSPVDCIPEE NNSAHPSFFSSSSKGDSFAQHL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
