Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00869094
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5952nts
Open reading frame: 1 to 5952
ATGAGTGGCAATAAAAGGGGTAGCAGAGCTTCCTGCCCTCACCGTGGTGCCGAGTGCCTG
CTGCCTTGGGCCGCCTTGAACCTCCAGGGTTTCCAGCTCCTCCTCCTTCACCCCAGTGCC
ACTGCCATGATGGATGTGAGTGAACTTGGGGAGTCTGCCCGCTACCTCCGCCAGGGCTAC
CAGGAGATGACGAAGGTGCACACTATCCCATGGGACGGGAAGAAGCGAGTCTGGGTGCCT
GATGAACAGGACGCCTACGTGGAGGCCGAGGTCAAGTCGGAGGCTACCGGGGGCAGAGTC
ACCGTGGAGACCAAAGACCAGAAGGTGCTGATGGTGCGTGAAGCCGAGCTGCAGCCCATG
AACCCGCCTCGCTTCGACTTACTGGAGGACATGGCCATGATGACGCACCTGAACGAGGCC
TCTGTGCTGCACAACCTGCGCCAGCGCTATGCCCGCTGGATGATCTATACCTACTCAGGC
CTCTTCTGTGTCACCATCAACCCCTACAAATGGCTCCCAGTCTATACGGCCTCCGTAGTG
GCTGCTTACAAGGGAAAGCGCCGCTCAGATTCCCCGCCCCATATATATGCGGTGGCGGAC
AACGCCTACAACGACATGCTGCGCAACCGAGACAACCAGTCCATGCTGATCACCGGAGAG
TCGGGGGCCGGTAAGACGGTTAACACCAAGCGGGTCATTCAGTACTTTGCCATCGTCGCT
GCCCTGGGAGACGGGCCGGGCAAGAAGGCCCAATTTCTGGCAACAAAGACGGGGGGCACC
CTTGAGGATCAAATCATCGAGGCCAACCCTGCCATGGAGGCCTTTGGCAACGCCAAGACC
CTGAGGAATGATAACTCCTCCCGCTTTGGCAAGTTCATCCGCATTCACTTTGGTCCCTCT
GGGAAGCTGGCATCCGCGGATATTGACAGCTATCTCCTGGAGAAGTCGCGGGTGATCTTC
CAGTTGCCTGGTGAGCGCAGCTACCATGTCTACTACCAGATCCTCTCAGGGAGGAAGCCA
GAGCTGCAGGACATGCTGCTTCTGTCTATGAACCCCTATGACTACCACTTCTGCAGCCAG
GGCGTCATCACCGTGGACAACATGAATGATGGGGAGGAGCTCATCGCCACCGACCATGCC
ATGGACATCCTAGGCTTCAGCGTGGATGAGAAATGTGCCTGCTATAAGATCGTGGGCGCC
CTCCTGCACTTTGGCAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGGCGGAGGCC
GATGGCACTGAGAGTGCTGACAAGGCTGCCTACCTGATGGGGGTCAGCAGTGGGGACCTC
CTCAAAGGCCTTTTGCACCCCCGGGTGCGTGTAGGGAACGAGTACGTGACCAAGGGCCAG
AGTGTGGAGCAGGTGGTGTTTGCTGTGGGGGCTCTGGCCAAGGCCACCTATGACCGGCTG
TTCAGGTGGCTGGTGTCTCGGATCAACCAGACCCTGGACACAAAGCTGCCCCGGCAGTTC
TTCATCGGGGTTCTGGACATCGCTGGGTTTGAGATCTTTGAGTTCAACAGCTTCGAACAG
CTGTGCATCAACTTCACCAATGAGAAATTGCAGCAGTTCTTCAACCAGCACATGTTTGTG
CTGGAGCAGGAGGAGTACAAGCGGGAGGGCATCGACTGGGTCTTCATCGACTTCGGCCTT
GACCTGCAGCCTTGCATCGACCTCATCGAGAAGCCACTGGGCATCCTGTCCATCCTGGAG
GAGGAATGCATGTTCCCCAAGGCCTCAGACGCCAGCTTCCGGGCCAAGCTCTACGACAAC
CACGCGGGGAAGTCACCCAATTTCCAGCAGCCTCGGCCTGACAAGAAGCGCAAGTACCAG
GCCCACTTCGAGGTGGTCCACTACGCAGGCGTGGTGCCTTACAGCATTGTGGGCTGGCTG
GAGAAAAACAAGGATCCCCTGAATGAGACCGTGGTCCCCATCTTCCAGAAGTCACAGAAT
AGGCTCCTGGCGACTCTCTATGAGAATTATGCGGGCTCCTGCTCCACTGAGCCCCCCAAG
TCTGGGGTGAAAGAGAAGCGTAAGAAGGCAGCATCGTTCCAGACGGTGTCCCAGCTGCAC
AAGGAGAACCTCAACAAGCTGATGACCAACCTGCGGGCCACACAGCCCCACTTCGTCCGC
TGCATTGTCCCCAACGAGAACAAAACCCCAGGGGTCATGGATGCCTTCTTGGTGCTACAC
CAGCTGCGCTGCAATGGGGTCCTGGAGGGGATCCGGATCTGCCGCCAAGGGTTCCCCAAC
AGGTTGCTCTACACCGACTTCCGGCAGCGGTACCGTATCCTGAACCCCAGTGCCATCCCG
GATGACACCTTCATGGACAGCAGGAAGGCCACAGAGAAACTGCTGGGCTCGCTGGACTTG
GATCACACCCAGTACCAGTTTGGCCACACCAAGGTGTTCTTCAAGGCTGGGCTTCTAGGC
GTCCTGGAAGAGCTCCGTGACCAGCGCCTGGCCAAGGTGCTGACGCTGCTGCAGGCGCGG
AGCCGTGGCCGCCTCATGCGCCTTGAGTACCAGCGCCTGCTGGGAGGCAGGGATGCGCTG
TTCACCATCCAGTGGAACATCCGTGCCTTCAATGCCGTCAAGAACTGGTCATGGATGAAG
CTCTTTTTCAAGATGAAGCCGCTGCTGCGCTCGGCGCAGGCTGAGGAGGAGCTGGCGGCC
CTGCGGGCAGAGCTGCGGGGGTTGCGAGGGGCGCTGGCTGCGGCCGAGGCCAAGCGCCAG
GAACTGGAGGAGACGCACGTCAGCATCACCCAGGAGAAGAATGACCTGGCCCTGCAGCTG
CAGGCTGAGCAGGACAACCTGGCAGATGCCGAGGAGCGCTGCCACTTGCTGATCAAGTCC
AAGGTGCAGCTGGAGGGGAAGGTGAAGGAGCTGAGTGAGCGGCTGGAGGATGAGGAGGAG
GTGAACGCTGACCTGGCCGCCCGCCGGCGCAAGCTGGAGGACGAGTGCACGGAGCTCAAG
AAGGACATTGATGACCTGGAGCTGACACTGGCCAAAGCTGAGAAGGAGAAGCAAGCCACT
GAGAACAAGGTGAAGAACCTGACGGAAGAGATGGCTGCGCTGGACGAGTCAGTGGCCCGG
CTGACCAAGGAGAAGAAGGCGTTGCAGGAGGCCCACCAACAGGCCCTGGGTGACCTGCAG
GCCGAGGAGGACCGTGTGAGCGCGCTGACCAAGGCCAAGCTCCGGCTGGAGCAACAGGTG
GAGGACCTGGAATGCTCCCTGGAGCAGGAGAAGAAGCTGCGCATGGACACGGAGCGGGCC
AAGCGCAAGCTGGAGGGTGACCTGAAGCTGACGCAGGAGTCGGTGGCTGATGCTGCTCAA
GACAAGCAGCAGCTGGAGGAGAAGCTCAAGAAGAAGGACTCCGAGCTGAGCCAGCTGAGC
CTGCGGGTGGAAGACGAGCAGCTCTTGGGGGCCCAGATGCAGAAGAAGATCAAGGAGCTG
CAGGCTCGGGCGGAGGAGCTGGAAGAGGAGCTGGAGGCAGAGCGGGCAGCCCGGGCCCGC
GTGGAGAAGCAGCGTGCAGAGGCGGCGCGGGAGCTGGAGGAGCTGAGCGAGCGGCTGGAG
GAGGCAGGCGGCGCATCCGCGGGGCAGCGCGAGGGCTGCCGCAAGCGGGAGGCGGAGCTG
GGGAGGCTGCGGCGGGAGCTGGAGGAGGCGGCGCTGCGGCACGAGGCCACAGTGGCGGCA
CTGCGGCGCAAGCAGGCGGAGGGCGCGGCGGAGCTGGGGGAGCAGGTGGACAGCCTGCAG
CGGGTGCGGCAGAAGCTGGAGAAGGAGAAGAGTGAGCTGCGCATGGAGGTGGACGACCTG
GCTGCCAACGTGGAGACTCTGACCCGCGCCAAGGCCAGTGCAGAGAAGCTGTGCCGGACC
TATGAGGATCAGCTAAGCGAGGCCAAGATCAAGGTGGAGGAGCTGCAGCGGCAGCTGGCG
GACGCAAGCACGCAGCGTGGGCGACTACAGACGGAAAGCGGGGAGCTGAGTCGCCTGCTA
GAGGAGAAGGAGTGTCTGATCAGTCAGCTGAGCCGTGGAAAGGCCCTGGCCGCCCAAAGC
CTGGAAGAGTTGCGGCGCCAGCTAGAGGAGGAAAGCAAGGCCAAGAGTGCCCTGGCCCAC
GCCGTGCAGGCTCTGCGGCACGACTGTGACCTCCTGCGGGAGCAACACGAGGAGGAGGCT
GAGGCCCAGGCTGAGCTGCAGCGGCTGCTGTCCAAGGCCAATGCCGAGGTGGCCCAGTGG
AGGAGCAAGTACGAAGCAGATGCCATCCAGAGGACCGAGGAGCTGGAGGAGGCCAAAAAA
AAGCTGGCACTGCGGCTGCAGGAGGCAGAGGAGGGCGTGGAGGCTGCCAACGCCAAGTGC
TCATCGTTGGAGAAGGCCAAGCTGCGGCTACAGACAGAGTCAGAGGATGTAACCCTGGAG
CTGGAGCGGGCGACCTCAGCAGCTGCTGCGCTGGACAAGAAGCAGCGGCACTTGGAACGG
GCACTGGAGGAACGGCGGCGGCAGGAGGAGGAGATGCAGCGGGAGCTGGAGGCGGCACAG
AGGGAGTCCCGTGGCCTGGGCACCGAGCTCTTCCGGCTGCGGCACGGCCACGAGGAGGCA
CTTGAAGCCCTGGAGACGCTCAAGCGGGAGAACAAGAACCTGCAGGAGGAGATCAGCGAC
CTCACAGACCAGGTGAGTCTCAGTGGGAAGAGCATCCAGGAACTGGAGAAAACCAAGAAG
GCGCTGGAAGGCGAGAAGAGTGAGATCCAGGCTGCACTGGAGGAGGCAGAGGGGGCCCTG
GAGCTGGAGGAGACCAAGACGCTGCGGATCCAGCTGGAGCTCTCCCAGGTCAAAGCAGAA
GTGGACCGGAAGCTGGCAGAGAAAGACGAGGAGTGCGCTAACCTGAGGCGCAACCACCAG
CGAGCTGTGGAGTCCCTGCAGGCCTCCCTGGATGCAGAGACACGGGCCCGCAATGAGGCG
CTGCGGCTCAAGAAGAAGATGGAGGGTGACCTCAACGACCTGGAGCTGCAGCTGGGCCAT
GCCACCCGTCAGGCCACAGAGGCCCAGGCTGCCACGCGGCTGATGCAGGCACAGCTCAAG
GAGGAGCAGGCAGGGCGGGACGAGGAGCAGCGGCTGGCAGCTGAGCTCCACGAGCAGGCG
CAGGCTCTGGAGCGCCGGGCCTCGCTGCTGGCTGCGGAGCTGGAGGAGCTGCGGGCTGCC
CTGGAGCAGGGCGAGCGCAGCCGGCGACTGGCAGAGCAGGAGCTTTTGGAGGCCACCGAG
CGCCTCAACCTTCTGCATTCGCAGAACACAGGCCTCCTAAACCAGAAGAAGAAGCTGGAG
GCGGACTTGGCCCAGCTGAGCGGGGAGGTGGAGGAGGCTGCACAGGAGAGGCGGGAGGCT
GAGGAGAAGGCCAAAAAGGCCATCACTGATGCGGCCATGATGGCCGAGGAGCTGAAGAAG
GAGCAGGACACAAGTGCACACCTGGAACGGATGAAGAAGACGCTGGAGCAGACGGTGCGC
GAGCTCCAGGCCCGCCTTGAGGAGGCAGAACAGGCCGCCCTCCGTGGCGGGAAGAAGCAG
GTGCAGAAGCTGGAGGCCAAGGTACGGGAGCTGGAGGCTGAGCTTGATGCAGAGCAGAAG
AAGCACGCCGAGGCCCTTAAGGGCGTGCGCAAGCATGAGCGCCGTGTCAAGGAGCTCGCA
TACCAGGCCGAGGAGGACAGGAAGAACCTGGCTCGCATGCAGGACCTGGTGGACAAGCTG
CAGAGCAAGGTCAAGAGCTACAAGCGCCAGTTTGAGGAGGCGGAGCAGCAGGCCAACACC
AACCTGGCCAAGTATCGCAAGGCCCAGCACGAGCTGGATGATGCGGAGGAGCGGGCAGAC
ATGGCGGAAACCCAGGCCAACAAGCTGCGGGCACGGACCCGGGACGCCCTGGGCCCCAAG
CACAAGGAGTTG
Protein sequence predicted based on nucleotide sequence
MSGNKRGSRASCPHRGAECLLPWAALNLQGFQLLLLHPSATAMMDVSELGESARYLRQGY QEMTKVHTIPWDGKKRVWVPDEQDAYVEAEVKSEATGGRVTVETKDQKVLMVREAELQPM NPPRFDLLEDMAMMTHLNEASVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTASVV AAYKGKRRSDSPPHIYAVADNAYNDMLRNRDNQSMLITGESGAGKTVNTKRVIQYFAIVA ALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPS GKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQ GVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEA DGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRL FRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFV LEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDN HAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQN RLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVR CIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIP DDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQAR SRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAA LRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKS KVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQAT ENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQV EDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLS LRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLE EAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQ RVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLA DASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAH AVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKK KLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLER ALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISD LTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAE VDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGH ATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAA LEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREA EEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQ VQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKL QSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPK HKEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
