Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00877122
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4344nts
Open reading frame: 1 to 4344
ATGTTTAGAGCTGGGGAGGCCTCCAAACGCCCATTGCCTGGGCCGTCGCCCCCAAGGGTG
CGGAGTGTGGAGGTTGCCCGGGGGAGGGCCGGCTACGGATTCACGCTTTCGGGACAGGCA
CCCTGTGTGCTCAGCTGCGTCATGAGAGGGAGCCCTGCGGATTTCGTGGGCCTCCGAGCT
GGAGACCAGATACTTGCTGTCAATGAAATCAACGTGAAAAAAGCATCTCATGAAGATGTA
GTGAAATTAATTGGGAAGTGCTCTGGTGTCCTTCACATGGTGATTGCTGAAGGCGTCGGC
CGCTTCGAATCCTGTTCCAGTGATGAAGAAGGGGGACTCTATGAAGGAAAAGGCTGGCTG
AAGCCCAAGCTGGATTCTAAAGCACTAGGTATAAACAGAGCAGAGCGAGTCGTGGAGGAA
ATGCAGTCTGGTGGAATTTTCAATATGATTTTTGAAAACCCGAGCCTTTGTGCGAGCAAT
TCAGAGCCCTTGAAATTGAAACAAAGATCCCTTTCAGAGTCGGCCGCAACTCGATTTGAT
GTTGGACATGAAAGTATAAATAATCCAAATCCCAACATGCTTTCTAAGGAGGAAATATCA
AAAGTTATTCATGATGATTCGGTTTTCAGCATTGGACTAGAAAGTCATGACGATTTTGCA
TTGGATGCAAGTATTTTAAACGTGGCGATGATCGTGGGCTACTTAGGCTCCATTGAGCTT
CCTTCCACGAGCTCCAACCTGGAGTCCGACAGCTTGCAAGCCATCCGCGGCTGCATGCGG
CGCCTGCGGGCAGAGCAGAAAATCCACTCGCTGGTGACCATGAAGATCATGCACGACTGT
GTGCAGCTGAGCACTGACAAGGCTGGAGTCGTGGCCGAGTACCCGGCCGAGAAGCTGGCC
TTCAGCGCCGTGTGCCCGGACGACCGGCGATTTTTCGGGTTGGTTACCATGCAGACGAAT
GACGACGGGAGCCTGGCCCAGGAGGAGGAGGGCGCCCTGCGGACTTCCTGCCACGTGTTC
ATGGTGGACCCAGACTTGTTTAATCACAAGATCCACCAAGGCATTGCTCGGCGGTTTGGG
TTTGAGTGCACGGCCGACCCAGACACCAATGGCTGTCTGGAATTCCCGGCGTCCTCCCTC
CCCGTCCTGCAGTTCATCTCTGTCCTGTACCGAGACATGGGTGAGCTGATTGAGGGCATG
CGGGCCCGCGCCTTTCTGGACGGGGACGCCGATGCCCACCAGAACAACAGCACCAGCAGC
AACAGTGACAGCGGCATTGGGAACTTCCACCAGGAGGAGAAGAGCAACCGGGTCCTTGTG
GTGGACCTGGGTGGGAGCTCGAGCAGACACGGCCCCGGAGGCAGCGCGTGGGACGGTGTG
GGTGGGAGGGGTGCCCAGCCCTGGGGTGCTCCCTGGACTGGGCCCTTCTGTCCGGACCCC
GAAGGGAGCCCCCCATTTGAGGCCGCTCATCAGACTGACAGGTTCTGGGACCTAAACAAG
CACCTAGGGCCAGCCTCTCCTGTGGAGGTGCCCCCAGCTTCCTTGAGGAGCTCAGTCCCC
CCTTCCAAGAGGGGCACCGTGGGTGCTGGCTGTGGTTTCAACCAGCGCTGGCTCCCGGTC
CACGTGCTCCGGGAGTGGCAGTGCGGACACACCAGCGACCAGGACTCTTACACAGATTCC
ACCGATGGCTGGTCCAGCATCAACTGCGGCACACTGCCCCCTCCTATGAGCAAGATCCCC
GCAGACCGCTACAGGGTGGAGGGCAGCTTCGCGCAGCCCCCGCTGAATGCCCCGAAGAGG
GAGTGGTCCAGGAAGGCCTTTGGAATGCAAAGCATTTTTGGTCCCCATCGAAATGTTCGA
AAGACTAAGGAAGATAAAAAGGGCTCAAAATTTGGGCGGGGAACTGGACTCACTCAGCCT
TCTCAACGCACGTCTGCTCGGAGATCATTTGGGAGATCCAAGAGATTCAGTATCACTCGC
TCCCTTGATGATCTTGAGTCTGCAACTGTGTCTGATGGCGAGTTGACGGGCGCCGACCTG
AAGGACTGCGTCAGCAACAACAGCCTGAGCAGCAATGCCAGCCTCCCCAGCGTGCAGAGC
TGCCGGCGCCTGCGTGAGAGGAGGGTCGCCAGCTGGGCCGTGTCCTTTGAGCGCCTGCTG
CAGGACCCCGTCGGTGTCCGCTACTTCTCTGATTTTCTAAGGAAAGAATTCAGTGAAGAA
AACATTTTATTCTGGCAGGCCTGTGAATATTTTAATCATGTTCCTGCACATGACAAAAAG
GAGCTTTCCTACAGGGCCCGGGAGATTTTCAGTAAGTTTCTCTGCAGCAAAGCCACCACC
CCGGTCAACATCGACAGCCAGGCCCAGCTAGCAGACGACGTCCTCCGCGCACCTCACCCA
GACATGTTCAAGGAGCAGCAGCTGCAGATCTTCAATCTCATGAAGTTTGATAGCTACACT
CGCTTTCTGAAGTCCCCGCTGTACCAGGAATGCATCCTGGCGGAAGTGGAGGGCCGTGCA
CTCCCGGACTCGCAGCAGGTCCCCAGCAGCCCGGCTTCCAAGCACAGCCTCGGTTCAGAC
CACTCCAGTGTGTCCACGCCAAAAAAGTTAAGTGGAAAATCAAAATCCGGCCGATCCCTG
AATGAAGAGCTGGGGGATGAGGACAGCGAGAAGAAGCGGAAAGGCGCGTTTTTCTCGTGG
TCGCGGACCAGGAGCACCGGGAGGTCCCAGAAAAAGAGGGAGCACGGGGACCACGCAGAC
GACGCCCTGCATGCCAATGGAGGCCTGTGTCGCCGAGAGTCGCAGGGCTCTGTGTCCTCT
GCGGGGAGCCTGGACCTGTCGGAGGCCTGCAGGACTTTGGCACCCGAGAAGGACAAGGCC
ACCAAGCACTGCTGCATTCATCTCCCGGATGGGACATCCTGCGTGGTGGCTGTCAAGGCG
GGCTTCTCCATCAAAGACATCCTGTCCGGACTCTGTGAGCGGCATGGCATCAACGGGGCG
GCCGCGGACCTCTTCCTGGTGGGCGGGGACAAGCCTCTGGTGCTGCACCAAGACAGTAGC
ATCTTGGAGTCAAGGGACCTGCGCCTAGAAAAGCGCACCTTGTTTCGGCTGGATCTTGTT
CCGATTAACCGGTCAGTGGGACTCAAGGCCAAGCCCACCAAGCCCGTCACGGAGGTGCTG
CGGCCCGTGGTGGCCAGATACGGCCTGGACCTCAGTGGCCTGCTGGTGAGGCTGAGTGGA
GAGAAGGAGCCCCTGGACCTTGGCGCCCCTATATCGAGTCTGGACGGACAGCGGGTTGTC
TTGGAGGAGAAGGATCCTTCCAGAGGAAAGGCATCCGCAGATAAACAGAAAGGTGTGCCA
GTGAAACAGAACACAGCTGTAAATTCCAGCTCCAGAAACCACTCGGCTACGGGAGAGGAA
AGAACACTAGGCAAGTCTAATTCTATTAAAATAAAAGGAGAAAATGGAAAAAATGCTAGG
GATCCCCGGCTTTCAAAGAGAGAAGAATCTATTGCAAAGATTGGGAAAAAAAAATATCAG
AAAATTAATTTGGACGAAGCAGAGGAGTTTTTTGAGCTTATTTCCAAAGCTCAGAGCAAC
AGAGCAGATGACCAACGTGGGCTGCTAAGGAAGGAAGACCTGGTGTTGCCAGAGTTCCTC
CGTTTACCTCCTGGTTCCACAGAACTCACCCTCCCCACTCCAGCTGCTGTGGCCAAGGGC
TTTAGCAAGAGAAGCGCCACAGGCAACGGCCGGGAGAGCGCCTCCCAGCCTGGCGAGCAG
TGGGAGCCAGTCCAGGAGAGCAGCGACAGCCCGTCCACCAGCCCGGGCTCAGCCTCCAGC
CCCCCTGGACCTCCTGGGACGACCCCCCCCGGGCAGAAGTCTCCCAGCGGGCCCTTCTGC
ACTCCCCAGTCCCCCGTCTCCCTCGCGCAGGAGGGCACCGCCCAGATCTGGAAGAGGCAG
TCTCAGGAAGTGGAGGCCGGGGGCATCCAGACGGTGGAGGATGAGCACGTGGCCGAGCTG
ACCCTGATGGGGGAGGGGGACATCAGCAGCCCCAACAGCACCTTGCTGCCGCCGCCCTCC
ACCCCCCAGGAAGTGCCAGGACCTTCCAGACCAGGAAGTGGGACCCATGGCAGCCGAGAC
CTCCCAGTCAACAGAATCATCGATGTGGATCTTGTAACTGGCTCGGCGCCCGGGCGGGAT
GGTGGCATAGCGGGGGCACAGGCTGGCCCTGGGAGGTCGCAGGCCAGTGGTGGGCCTCCT
ACATCAGACCTCCCTGGCTTGGGCCCCGTCCCGGGTGAGCCTGCTAAGCCCAAGACCAGC
GCTCACCACGCCACCTTCGTCTTG
Protein sequence predicted based on nucleotide sequence
MFRAGEASKRPLPGPSPPRVRSVEVARGRAGYGFTLSGQAPCVLSCVMRGSPADFVGLRA GDQILAVNEINVKKASHEDVVKLIGKCSGVLHMVIAEGVGRFESCSSDEEGGLYEGKGWL KPKLDSKALGINRAERVVEEMQSGGIFNMIFENPSLCASNSEPLKLKQRSLSESAATRFD VGHESINNPNPNMLSKEEISKVIHDDSVFSIGLESHDDFALDASILNVAMIVGYLGSIEL PSTSSNLESDSLQAIRGCMRRLRAEQKIHSLVTMKIMHDCVQLSTDKAGVVAEYPAEKLA FSAVCPDDRRFFGLVTMQTNDDGSLAQEEEGALRTSCHVFMVDPDLFNHKIHQGIARRFG FECTADPDTNGCLEFPASSLPVLQFISVLYRDMGELIEGMRARAFLDGDADAHQNNSTSS NSDSGIGNFHQEEKSNRVLVVDLGGSSSRHGPGGSAWDGVGGRGAQPWGAPWTGPFCPDP EGSPPFEAAHQTDRFWDLNKHLGPASPVEVPPASLRSSVPPSKRGTVGAGCGFNQRWLPV HVLREWQCGHTSDQDSYTDSTDGWSSINCGTLPPPMSKIPADRYRVEGSFAQPPLNAPKR EWSRKAFGMQSIFGPHRNVRKTKEDKKGSKFGRGTGLTQPSQRTSARRSFGRSKRFSITR SLDDLESATVSDGELTGADLKDCVSNNSLSSNASLPSVQSCRRLRERRVASWAVSFERLL QDPVGVRYFSDFLRKEFSEENILFWQACEYFNHVPAHDKKELSYRAREIFSKFLCSKATT PVNIDSQAQLADDVLRAPHPDMFKEQQLQIFNLMKFDSYTRFLKSPLYQECILAEVEGRA LPDSQQVPSSPASKHSLGSDHSSVSTPKKLSGKSKSGRSLNEELGDEDSEKKRKGAFFSW SRTRSTGRSQKKREHGDHADDALHANGGLCRRESQGSVSSAGSLDLSEACRTLAPEKDKA TKHCCIHLPDGTSCVVAVKAGFSIKDILSGLCERHGINGAAADLFLVGGDKPLVLHQDSS ILESRDLRLEKRTLFRLDLVPINRSVGLKAKPTKPVTEVLRPVVARYGLDLSGLLVRLSG EKEPLDLGAPISSLDGQRVVLEEKDPSRGKASADKQKGVPVKQNTAVNSSSRNHSATGEE RTLGKSNSIKIKGENGKNARDPRLSKREESIAKIGKKKYQKINLDEAEEFFELISKAQSN RADDQRGLLRKEDLVLPEFLRLPPGSTELTLPTPAAVAKGFSKRSATGNGRESASQPGEQ WEPVQESSDSPSTSPGSASSPPGPPGTTPPGQKSPSGPFCTPQSPVSLAQEGTAQIWKRQ SQEVEAGGIQTVEDEHVAELTLMGEGDISSPNSTLLPPPSTPQEVPGPSRPGSGTHGSRD LPVNRIIDVDLVTGSAPGRDGGIAGAQAGPGRSQASGGPPTSDLPGLGPVPGEPAKPKTS AHHATFVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
