Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00877140
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5952nts
Open reading frame: 1 to 5952
ATGAGTGGCAATAAAAGGGGTAGCAGAGCTTCCTGCCCTCACCGTGGTGCCGAGTGCCTG
CTGCCTTGGGCCGCCTTGAACCTCCAGGGTTTCCAGCTCCTCCTCCTTCACCCCAGTGCC
ACTGCCATGATGGATGTGAGTGAACTTGGGGAGTCTGCCCGCTACCTCCGCCAGGGCTAC
CAGGAGATGACGAAGGTGCACACTATCCCATGGGACGGGAAGAAGCGAGTCTGGGTGCCT
GATGAACAGGACGCCTACGTGGAGGCCGAGGTCAAGTCGGAGGCTACCGGGGGCAGAGTC
ACCGTGGAGACCAAAGACCAGAAGGTGCTGATGGTGCGTGAAGCCGAGCTGCAGCCCATG
AACCCGCCTCGCTTCGACTTACTGGAGGACATGGCCATGATGACGCACCTGAACGAGGCC
TCTGTGCTGCACAACCTGCGCCAGCGCTATGCCCGCTGGATGATCTATACCTACTCAGGC
CTCTTCTGTGTCACCATCAACCCCTACAAATGGCTCCCAGTCTATACGGCCTCCGTAGTG
GCTGCTTACAAGGGAAAGCGCCGCTCAGATTCCCCGCCCCATATATATGCGGTGGCGGAC
AACGCCTACAACGACATGCTGCGCAACCGAGACAACCAGTCCATGCTGATCACCGGAGAG
TCGGGGGCCGGTAAGACGGTTAACACCAAGCGGGTCATTCAGTACTTTGCCATCGTCGCT
GCCCTGGGAGACGGGCCGGGCAAGAAGGCCCAATTTCTGGCAACAAAGACGGGGGGCACC
CTTGAGGATCAAATCATCGAGGCCAACCCTGCCATGGAGGCCTTTGGCAACGCCAAGACC
CTGAGGAATGATAACTCCTCCCGCTTTGGCAAGTTCATCCGCATTCACTTTGGTCCCTCT
GGGAAGCTGGCATCCGCGGATATTGACAGCTATCTCCTGGAGAAGTCGCGGGTGATCTTC
CAGTTGCCTGGTGAGCGCAGCTACCATGTCTACTACCAGATCCTCTCAGGGAGGAAGCCA
GAGCTGCAGGACATGCTGCTTCTGTCTATGAACCCCTATGACTACCACTTCTGCAGCCAG
GGCGTCATCACCGTGGACAACATGAATGATGGGGAGGAGCTCATCGCCACCGACCATGCC
ATGGACATCCTAGGCTTCAGCGTGGATGAGAAATGTGCCTGCTATAAGATCGTGGGCGCC
CTCCTGCACTTTGGCAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGGCGGAGGCC
GATGGCACTGAGAGTGCTGACAAGGCTGCCTACCTGATGGGGGTCAGCAGTGGGGACCTC
CTCAAAGGCCTTTTGCACCCCCGGGTGCGTGTAGGGAACGAGTACGTGACCAAGGGCCAG
AGTGTGGAGCAGGTGGTGTTTGCTGTGGGGGCTCTGGCCAAGGCCACCTATGACCGGCTG
TTCAGGTGGCTGGTGTCTCGGATCAACCAGACCCTGGACACAAAGCTGCCCCGGCAGTTC
TTCATCGGGGTTCTGGACATCGCTGGGTTTGAGATCTTTGAGTTCAACAGCTTCGAACAG
CTGTGCATCAACTTCACCAATGAGAAATTGCAGCAGTTCTTCAACCAGCACATGTTTGTG
CTGGAGCAGGAGGAGTACAAGCGGGAGGGCATCGACTGGGTCTTCATCGACTTCGGCCTT
GACCTGCAGCCTTGCATCGACCTCATCGAGAAGCCACTGGGCATCCTGTCCATCCTGGAG
GAGGAATGCATGTTCCCCAAGGCCTCAGACGCCAGCTTCCGGGCCAAGCTCTACGACAAC
CACGCGGGGAAGTCACCCAATTTCCAGCAGCCTCGGCCTGACAAGAAGCGCAAGTACCAG
GCCCACTTCGAGGTGGTCCACTACGCAGGCGTGGTGCCTTACAGCATTGTGGGCTGGCTG
GAGAAAAACAAGGATCCCCTGAATGAGACCGTGGTCCCCATCTTCCAGAAGTCACAGAAT
AGGCTCCTGGCGACTCTCTATGAGAATTATGCGGGCTCCTGCTCCACTGAGCCCCCCAAG
TCTGGGGTGAAAGAGAAGCGTAAGAAGGCAGCATCGTTCCAGACGGTGTCCCAGCTGCAC
AAGGAGAACCTCAACAAGCTGATGACCAACCTGCGGGCCACACAGCCCCACTTCGTCCGC
TGCATTGTCCCCAACGAGAACAAAACCCCAGGGGTCATGGATGCCTTCTTGGTGCTACAC
CAGCTGCGCTGCAATGGGGTCCTGGAGGGGATCCGGATCTGCCGCCAAGGGTTCCCCAAC
AGGTTGCTCTACACCGACTTCCGGCAGCGGTACCGTATCCTGAACCCCAGTGCCATCCCG
GATGACACCTTCATGGACAGCAGGAAGGCCACAGAGAAACTGCTGGGCTCGCTGGACTTG
GATCACACCCAGTACCAGTTTGGCCACACCAAGGTGTTCTTCAAGGCTGGGCTTCTAGGC
GTCCTGGAAGAGCTCCGTGACCAGCGCCTGGCCAAGGTGCTGACGCTGCTGCAGGCGCGG
AGCCGTGGCCGCCTCATGCGCCTTGAGTACCAGCGCCTGCTGGGAGGCAGGGATGCGCTG
TTCACCATCCAGTGGAACATCCGTGCCTTCAATGCCGTCAAGAACTGGTCATGGATGAAG
CTCTTTTTCAAGATGAAGCCGCTGCTGCGCTCGGCGCAGGCTGAGGAGGAGCTGGCGGCC
CTGCGGGCAGAGCTGCGGGGGTTGCGAGGGGCGCTGGCTGCGGCCGAGGCCAAGCGCCAG
GAACTGGAGGAGACGCACGTCAGCATCACCCAGGAGAAGAATGACCTGGCCCTGCAGCTG
CAGGCTGAGCAGGACAACCTGGCAGATGCCGAGGAGCGCTGCCACTTGCTGATCAAGTCC
AAGGTGCAGCTGGAGGGGAAGGTGAAGGAGCTGAGTGAGCGGCTGGAGGATGAGGAGGAG
GTGAACGCTGACCTGGCCGCCCGCCGGCGCAAGCTGGAGGACGAGTGCACGGAGCTCAAG
AAGGACATTGATGACCTGGAGCTGACACTGGCCAAAGCTGAGAAGGAGAAGCAAGCCACT
GAGAACAAGGTGAAGAACCTGACGGAAGAGATGGCTGCGCTGGACGAGTCAGTGGCCCGG
CTGACCAAGGAGAAGAAGGCGTTGCAGGAGGCCCACCAACAGGCCCTGGGTGACCTGCAG
GCCGAGGAGGACCGTGTGAGCGCGCTGACCAAGGCCAAGCTCCGGCTGGAGCAACAGGTG
GAGGACCTGGAATGCTCCCTGGAGCAGGAGAAGAAGCTGCGCATGGACACGGAGCGGGCC
AAGCGCAAGCTGGAGGGTGACCTGAAGCTGACGCAGGAGTCGGTGGCTGATGCTGCTCAA
GACAAGCAGCAGCTGGAGGAGAAGCTCAAGAAGAAGGACTCCGAGCTGAGCCAGCTGAGC
CTGCGGGTGGAAGACGAGCAGCTCTTGGGGGCCCAGATGCAGAAGAAGATCAAGGAGCTG
CAGGCTCGGGCGGAGGAGCTGGAAGAGGAGCTGGAGGCAGAGCGGGCAGCCCGGGCCCGC
GTGGAGAAGCAGCGTGCAGAGGCGGCGCGGGAGCTGGAGGAGCTGAGCGAGCGGCTGGAG
GAGGCAGGCGGCGCATCCGCGGGGCAGCGCGAGGGCTGCCGCAAGCGGGAGGCGGAGCTG
GGGAGGCTGCGGCGGGAGCTGGAGGAGGCGGCGCTGCGGCACGAGGCCACAGTGGCGGCA
CTGCGGCGCAAGCAGGCGGAGGGCGCGGCGGAGCTGGGGGAGCAGGTGGACAGCCTGCAG
CGGGTGCGGCAGAAGCTGGAGAAGGAGAAGAGTGAGCTGCGCATGGAGGTGGACGACCTG
GCTGCCAACGTGGAGACTCTGACCCGCGCCAAGGCCAGTGCAGAGAAGCTGTGCCGGACC
TATGAGGATCAGCTAAGCGAGGCCAAGATCAAGGTGGAGGAGCTGCAGCGGCAGCTGGCG
GACGCAAGCACGCAGCGTGGGCGACTACAGACGGAAAGCGGGGAGCTGAGTCGCCTGCTA
GAGGAGAAGGAGTGTCTGATCAGTCAGCTGAGCCGTGGAAAGGCCCTGGCCGCCCAAAGC
CTGGAAGAGTTGCGGCGCCAGCTAGAGGAGGAAAGCAAGGCCAAGAGTGCCCTGGCCCAC
GCCGTGCAGGCTCTGCGGCACGACTGTGACCTCCTGCGGGAGCAACACGAGGAGGAGGCT
GAGGCCCAGGCTGAGCTGCAGCGGCTGCTGTCCAAGGCCAATGCCGAGGTGGCCCAGTGG
AGGAGCAAGTACGAAGCAGATGCCATCCAGAGGACCGAGGAGCTGGAGGAGGCCAAAAAA
AAGCTGGCACTGCGGCTGCAGGAGGCAGAGGAGGGCGTGGAGGCTGCCAACGCCAAGTGC
TCATCGTTGGAGAAGGCCAAGCTGCGGCTACAGACAGAGTCAGAGGATGTAACCCTGGAG
CTGGAGCGGGCGACCTCAGCAGCTGCTGCGCTGGACAAGAAGCAGCGGCACTTGGAACGG
GCACTGGAGGAACGGCGGCGGCAGGAGGAGGAGATGCAGCGGGAGCTGGAGGCGGCACAG
AGGGAGTCCCGTGGCCTGGGCACCGAGCTCTTCCGGCTGCGGCACGGCCACGAGGAGGCA
CTTGAAGCCCTGGAGACGCTCAAGCGGGAGAACAAGAACCTGCAGGAGGAGATCAGCGAC
CTCACAGACCAGGTGAGTCTCAGTGGGAAGAGCATCCAGGAACTGGAGAAAACCAAGAAG
GCGCTGGAAGGCGAGAAGAGTGAGATCCAGGCTGCACTGGAGGAGGCAGAGGGGGCCCTG
GAGCTGGAGGAGACCAAGACGCTGCGGATCCAGCTGGAGCTCTCCCAGGTCAAAGCAGAA
GTGGACCGGAAGCTGGCAGAGAAAGACGAGGAGTGCGCTAACCTGAGGCGCAACCACCAG
CGAGCTGTGGAGTCCCTGCAGGCCTCCCTGGATGCAGAGACACGGGCCCGCAATGAGGCG
CTGCGGCTCAAGAAGAAGATGGAGGGTGACCTCAACGACCTGGAGCTGCAGCTGGGCCAT
GCCACCCGTCAGGCCACAGAGGCCCAGGCTGCCACGCGGCTGATGCAGGCACAGCTCAAG
GAGGAGCAGGCAGGGCGGGACGAGGAGCAGCGGCTGGCAGCTGAGCTCCACGAGCAGGCG
CAGGCTCTGGAGCGCCGGGCCTCGCTGCTGGCTGCGGAGCTGGAGGAGCTGCGGGCTGCC
CTGGAGCAGGGCGAGCGCAGCCGGCGACTGGCAGAGCAGGAGCTTTTGGAGGCCACCGAG
CGCCTCAACCTTCTGCATTCGCAGAACACAGGCCTCCTAAACCAGAAGAAGAAGCTGGAG
GCGGACTTGGCCCAGCTGAGCGGGGAGGTGGAGGAGGCTGCACAGGAGAGGCGGGAGGCT
GAGGAGAAGGCCAAAAAGGCCATCACTGATGCGGCCATGATGGCCGAGGAGCTGAAGAAG
GAGCAGGACACAAGTGCACACCTGGAACGGATGAAGAAGACGCTGGAGCAGACGGTGCGC
GAGCTCCAGGCCCGCCTTGAGGAGGCAGAACAGGCCGCCCTCCGTGGCGGGAAGAAGCAG
GTGCAGAAGCTGGAGGCCAAGGTACGGGAGCTGGAGGCTGAGCTTGATGCAGAGCAGAAG
AAGCACGCCGAGGCCCTTAAGGGCGTGCGCAAGCATGAGCGCCGTGTCAAGGAGCTCGCA
TACCAGGCCGAGGAGGACAGGAAGAACCTGGCTCGCATGCAGGACCTGGTGGACAAGCTG
CAGAGCAAGGTCAAGAGCTACAAGCGCCAGTTTGAGGAGGCGGAGCAGCAGGCCAACACC
AACCTGGCCAAGTATCGCAAGGCCCAGCACGAGCTGGATGATGCGGAGGAGCGGGCAGAC
ATGGCGGAAACCCAGGCCAACAAGCTGCGGGCACGGACCCGGGACGCCCTGGGCCCCAAG
CACAAGGAGTTG
Protein sequence predicted based on nucleotide sequence
MSGNKRGSRASCPHRGAECLLPWAALNLQGFQLLLLHPSATAMMDVSELGESARYLRQGY QEMTKVHTIPWDGKKRVWVPDEQDAYVEAEVKSEATGGRVTVETKDQKVLMVREAELQPM NPPRFDLLEDMAMMTHLNEASVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTASVV AAYKGKRRSDSPPHIYAVADNAYNDMLRNRDNQSMLITGESGAGKTVNTKRVIQYFAIVA ALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPS GKLASADIDSYLLEKSRVIFQLPGERSYHVYYQILSGRKPELQDMLLLSMNPYDYHFCSQ GVITVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEA DGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRL FRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFV LEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDN HAGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQN RLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVR CIVPNENKTPGVMDAFLVLHQLRCNGVLEGIRICRQGFPNRLLYTDFRQRYRILNPSAIP DDTFMDSRKATEKLLGSLDLDHTQYQFGHTKVFFKAGLLGVLEELRDQRLAKVLTLLQAR SRGRLMRLEYQRLLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAA LRAELRGLRGALAAAEAKRQELEETHVSITQEKNDLALQLQAEQDNLADAEERCHLLIKS KVQLEGKVKELSERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQAT ENKVKNLTEEMAALDESVARLTKEKKALQEAHQQALGDLQAEEDRVSALTKAKLRLEQQV EDLECSLEQEKKLRMDTERAKRKLEGDLKLTQESVADAAQDKQQLEEKLKKKDSELSQLS LRVEDEQLLGAQMQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLE EAGGASAGQREGCRKREAELGRLRRELEEAALRHEATVAALRRKQAEGAAELGEQVDSLQ RVRQKLEKEKSELRMEVDDLAANVETLTRAKASAEKLCRTYEDQLSEAKIKVEELQRQLA DASTQRGRLQTESGELSRLLEEKECLISQLSRGKALAAQSLEELRRQLEEESKAKSALAH AVQALRHDCDLLREQHEEEAEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKK KLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLER ALEERRRQEEEMQRELEAAQRESRGLGTELFRLRHGHEEALEALETLKRENKNLQEEISD LTDQVSLSGKSIQELEKTKKALEGEKSEIQAALEEAEGALELEETKTLRIQLELSQVKAE VDRKLAEKDEECANLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGH ATRQATEAQAATRLMQAQLKEEQAGRDEEQRLAAELHEQAQALERRASLLAAELEELRAA LEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEADLAQLSGEVEEAAQERREA EEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQ VQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQAEEDRKNLARMQDLVDKL QSKVKSYKRQFEEAEQQANTNLAKYRKAQHELDDAEERADMAETQANKLRARTRDALGPK HKEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
