Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878146
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3912nts
Open reading frame: 1 to 3912
ATGCCCGGGCCCCGAGGGGCTGCTGGCGGCCTGGCCCCTGAGATGCGCGGGGCGGGGGCG
GCGGGGCTGCTGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGGGCCTGGGCGGCAGG
GTCGAGGGGGGGCCGGCCGGCGAGCGGGGCGCAGGCGGGGGCGGGGCGCTGGCCCGCGAG
CGCTTCAAGGTGGTCTTTGCGCCGGTGATCTGCAAGCGGACCTGTCTCAAGGGCCAGTGT
CGGGACAGTTGTCAGCAGGGCTCCAACATGACGCTCATCGGAGAGAACGGCCACAGCACA
GACACGCTCACGGGCTCCGGCTTCCGCGTGGTGGTGTGCCCTCTCCCCTGCATGAATGGC
GGCCAGTGCTCCTCGCGAAACCAGTGCCTGTGTCCCCCGGACTTCACTGGGCGCTTCTGC
CAGGTGCCCGCAGGAGGAGCCGGTGGGGGTACCGGCGGCTCAGGCCCCGGCCTGAGCAGG
ACAGGGGCCCTGTCCACAGGGGCGCTGCCGCCCCTGGCTCCGGAGGGCGACTCTGTGGCC
AGCAAGCACGCCATCTACGCCGTCCAGGTGATCGCTGACCCTCCTGGGCCCGGGGAGGGG
CCTCCTGCCCAGCACGCAGCCTTCCTGGTGCCCCTAGGCCCGGGACAGATCTCAGCAGAA
GTGCAGGCCCCGCCCCCCGTGGTGAATGTGCGCGTCCATCACCCGCCCGAGGCCTCAGTC
CAGGTGCACCGCATTGAGAGCTCGAACGCCGAGAGCGCAGCCCCCTCCCAGCACCTGCTG
CCGCACCCCAAGCCCTCGCACCCCCGGCCGCCCACCCAGAAGCCCCTGGGCCGCTGCTTT
CAGGACACTCTGCCCAAGCAGCCGTGTGGCAGCAACCCCCTCCCCGGCCTCACCAAGCAG
GAAGACTGCTGCGGTAGCATCGGCACTGCCTGGGGCCAGAGCAAGTGCCACAAGTGTCCC
CAGCTGCAGTACACAGGAGTGCAGAAGCCAGGGCCTGTACGTGGGGAAGTGGGCGCTGAC
TGTCCCCAGGGCTACAAGAGGCTTAACAGCACCCACTGCCAGGACATCAACGAGTGCGCA
ATGCCGGGCGTGTGTCGCCATGGTGACTGCCTCAACAACCCTGGCTCCTATCGCTGTGTC
TGCCCACCTGGCCATAGTTTAGGCCCCTCCCGTACACAGTGCATTGCAGACAAACCGGAG
GAGAAGAGCCTGTGTTTCCGCCTGGTGAGCCCTGAGCACCAGTGCCAGCACCCACTGACC
ACCCGCCTGACCCGCCAGCTCTGCTGCTGCAGTGTCGGCAAGGCCTGGGGCGCGCGGTGT
CAGCGCTGCCCAACAGATGGCACCGCTGCGTTCAAGGAGATCTGCCCAGCTGGGAAGGGA
TACCACATTCTCACCTCCCACCAGACGCTCACCATTCAGGGCGAGAGTGACTTTTCCCTT
TTCCTGCACCCTGACGGGCCACCCAAGCCCCAGCAGCTTCCGGAGAGCCCTAGCCAGGCT
CCACCACCTGAGGACACAGAGGAAGAGAGAGGGGTGACCACGGACTCACCGGTGAGTGAG
GAGAGGTCAGTGCAGCAGAGCCACCCAACTGCCACCACGACTCCTGCCCGGCCCTACCCC
GAGCTGATCTCCCGTCCCTCGCCCCCGACCATGCGCTGGTTCCTGCCGGACTTGCCTCCT
TCCCGCAGCGCCGTAGAGATCGCTCCCACTCAGGTCACAGAGACTGATGAGTGCCGACTG
AACCAGAACATCTGTGGCCACGGAGAGTGCGTGCCGGGCCCCCCTGACTACTCCTGCCAC
TGCAACCCCGGCTACCGGTCACATCCCCAGCACCGCTACTGCGTGGATGTGAACGAGTGC
GAGGCAGAGCCCTGTGGCCCGGGGAGGGGCATCTGCATGAACACCGGCGGCTCCTACAAT
TGCCACTGCAACCGCGGCTACCGCCTGCACGTGGGCGCCGGGGGGCGCTCGTGCGTGGAC
CTGAACGAATGCGCCAAGCCCCACCTGTGCGGCGACGGCGGCTTCTGCATCAACTTTCCC
GGTCACTACAAGTGCAACTGCTACCCCGGCTACCGGCTCAAAGCCTCCCGGCCTCCTGTG
TGCGAAGACATCGACGAGTGCCGGGACCCAAGCTCTTGCCCGGATGGCAAATGCGAGAAC
AAGCCCGGGAGCTTCAAGTGCATCGCCTGTCAGCCTGGCTACCGCAGCCAGGGGGGCGGG
GCCTGTCGCGACGTGAACGAGTGCGCCGAGGGCAGCCCCTGCTCGCCTGGCTGGTGCGAG
AACCTCCCGGGCTCCTTCCGCTGCACCTGTGCCCAGGGCTACGCGCCCGCGCCCGACGGC
CGCAGTTGCTTGGATGTGGACGAGTGTGAGGCTGGGGACGTGTGTGACAATGGCATCTGC
AGCAACACGCCAGGATCTTTCCAGTGTCAGTGCCTCTCTGGCTACCATCTGTCCAGGGAC
CGGAGCCACTGCGAGGACATTGATGAGTGTGACTTCCCTGCAGCCTGCATTGGGGGTGAC
TGCATCAATACCAATGGCTCCTACAGATGTCTTTGCCCCCAGGGGCATCGGCTGGTGGGT
GGCAGGAAATGCCAAGACATAGATGAGTGCAGCCAGGACCCGAGCCTGTGCCTTCCCCAT
GGGGCCTGCAAGAACCTTCAGGGCTCCTATGTGTGTGTCTGCGATGAGGGCTTCACTCCC
ACCCAGGACCAGCACGGTTGTGAGGAGGTGGAGCAGCCCCACCACAAGAAGGAGTGCTAC
CTGAACTTCGATGACACAGTGTTCTGCGACAGCGTATTGGCCACCAACGTGACCCAGCAG
GAGTGCTGCTGCTCTCTGGGGGCCGGCTGGGGCGACCACTGCGAAATCTACCCCTGCCCA
GTCTACAGCTCAGCCGAGTTCCACAGCCTCTGCCCAGACGGAAAGGGCTACACCCAGGAC
AACAACATCGTCAACTACGGCATCCCAGCCCACCGTGACATCGACGAGTGCATGTTGTTC
GGGTCGGAGATTTGCAAGGAGGGCAAGTGCGTGAACACGCAGCCTGGCTACGAGTGCTAC
TGCAAGCAGGGCTTCTACTACGACGGGAACCTGCTGGAATGCGTGGACGTGGACGAGTGC
CTGGACGAGTCCAACTGCCGGAACGGAGTGTGTGAGAACACGCGCGGCGGCTACCGCTGT
GCCTGCACGCCCCCTGCCGAGTACAGTCCCGCGCAGCGCCAGTGCCTGAGCCCGGAAGAG
ATGGACGTGGACGAGTGCCAGGACCCGGCAGCCTGCCGCCCTGGCCGCTGCGTCAACCTG
CCGGGCTCCTACCGCTGCGAGTGTCGCCCGCCCTGGGTGCCCGGGCCCTCCGGCCGCGAT
TGCCAGCTCCCCGAGAGCCCGGCCGAGCGTGCCCCGGAGCGGCGCGACGTGTGCTGGAGC
CAGCGCGGAGAGGACGGCATGTGCGCTGGCCCCCTGGCCGGGCCTGCCCTCACCTTCGAC
GACTGCTGCTGCCGCCAGGGCCGCGGCTGGGGCGCCCAATGCCGACCGTGCCCGCCGCGC
GGCGCGGGGTCCCATTGCCCGACATCGCAGAGCGAGAGCAATTCCTTCTGGGACACAAGC
CCCCTGCTGTTGGGGAAGCCCCCAAGAGATGAGGACAGTTCAGAGGAGGATTCAGACGAG
TGTCGCTGCGTGAGTGGCCGCTGCGTGCCGCGGCCGGGCGGCGCCGTGTGCGAGTGTCCC
GGCGGCTTCCAGCTCGACGCCTCCCGCGCCCGCTGCGTGGATATCGACGAGTGCCGAGAG
CTGAACCAGCGCGGGCTGCTGTGCAAGAGCGAGCGCTGCGTGAACACCAGCGGCTCCTTC
CGCTGCGTCTGCAAAGCCGGCTTCGCGCGCAGCCGCCCGCACGGGGCCTGCGTTCCCCAG
CGCCGCCGCTTG
Protein sequence predicted based on nucleotide sequence
MPGPRGAAGGLAPEMRGAGAAGLLALLLLLLLLLLGLGGRVEGGPAGERGAGGGGALARE RFKVVFAPVICKRTCLKGQCRDSCQQGSNMTLIGENGHSTDTLTGSGFRVVVCPLPCMNG GQCSSRNQCLCPPDFTGRFCQVPAGGAGGGTGGSGPGLSRTGALSTGALPPLAPEGDSVA SKHAIYAVQVIADPPGPGEGPPAQHAAFLVPLGPGQISAEVQAPPPVVNVRVHHPPEASV QVHRIESSNAESAAPSQHLLPHPKPSHPRPPTQKPLGRCFQDTLPKQPCGSNPLPGLTKQ EDCCGSIGTAWGQSKCHKCPQLQYTGVQKPGPVRGEVGADCPQGYKRLNSTHCQDINECA MPGVCRHGDCLNNPGSYRCVCPPGHSLGPSRTQCIADKPEEKSLCFRLVSPEHQCQHPLT TRLTRQLCCCSVGKAWGARCQRCPTDGTAAFKEICPAGKGYHILTSHQTLTIQGESDFSL FLHPDGPPKPQQLPESPSQAPPPEDTEEERGVTTDSPVSEERSVQQSHPTATTTPARPYP ELISRPSPPTMRWFLPDLPPSRSAVEIAPTQVTETDECRLNQNICGHGECVPGPPDYSCH CNPGYRSHPQHRYCVDVNECEAEPCGPGRGICMNTGGSYNCHCNRGYRLHVGAGGRSCVD LNECAKPHLCGDGGFCINFPGHYKCNCYPGYRLKASRPPVCEDIDECRDPSSCPDGKCEN KPGSFKCIACQPGYRSQGGGACRDVNECAEGSPCSPGWCENLPGSFRCTCAQGYAPAPDG RSCLDVDECEAGDVCDNGICSNTPGSFQCQCLSGYHLSRDRSHCEDIDECDFPAACIGGD CINTNGSYRCLCPQGHRLVGGRKCQDIDECSQDPSLCLPHGACKNLQGSYVCVCDEGFTP TQDQHGCEEVEQPHHKKECYLNFDDTVFCDSVLATNVTQQECCCSLGAGWGDHCEIYPCP VYSSAEFHSLCPDGKGYTQDNNIVNYGIPAHRDIDECMLFGSEICKEGKCVNTQPGYECY CKQGFYYDGNLLECVDVDECLDESNCRNGVCENTRGGYRCACTPPAEYSPAQRQCLSPEE MDVDECQDPAACRPGRCVNLPGSYRCECRPPWVPGPSGRDCQLPESPAERAPERRDVCWS QRGEDGMCAGPLAGPALTFDDCCCRQGRGWGAQCRPCPPRGAGSHCPTSQSESNSFWDTS PLLLGKPPRDEDSSEEDSDECRCVSGRCVPRPGGAVCECPGGFQLDASRARCVDIDECRE LNQRGLLCKSERCVNTSGSFRCVCKAGFARSRPHGACVPQRRRL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
