Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878151
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6351nts
Open reading frame: 1 to 6351
ATGTCGGGGTTCAGGCTGGGTGACCACGTGTGGCTGGAGCCTCCCTCCACCCACAAGACC
GGCGTGGCCATCGGGGGCATCATCAAAGAGGCAAAGCCAGGCAAAGTCTTGGTTGAAGAT
GACGAGGGCAAGGAACACTGGATCCGAGCAGAGGACTTTGGTGTCCTCAGTCCCATGCAC
CCCAACTCAGTCCAGGGTGTGGACGACATGATCCGCCTGGGGGACCTGAACGAGGCAGGC
ATGGTGCACAACCTCCTGATCCGCTACCAGCAGCACAAGATCTATACATACACAGGCTCC
ATCCTGGTGGCCGTCAACCCGTTCCAGGTGCTGCCGCTCTACACCCTGGAGCAGGTACAG
CTCTACTACAGCCGCCATATGGGCGAGCTGCCCCCGCATGTCTTTGCCATCGCCAACAAC
TGCTACTTCAGCATGAAGAGGAACAAGAGGGACCAGTGCTGCATCATCAGCGGCGAGTCT
GGGGCTGGCAAGACGGAGACCACCAAGCTCATCCTGCAGTTCCTGGCCACCATCAGTGGC
CAGCATTCGTGGATTGAGCAGCAGGTCCTGGAAGCCAACCCCATCCTGGAGGCCTTTGGA
AATGCCAAGACAATCCGCAACGACAACTCAAGCCGCTTTGGGAAGTACATTGACATCTAC
TTTAACCCCAGCGGGGTGATCGAGGGCGCGCGCATCGAGCAATTTCTCCTGGAGAAGTCC
CGGGTCTGCCGGCAGGCTCCCGAGGAGCGGAACTACCATATCTTCTACTGCATGCTCATG
GGGGTGAGTGCTGAGGACAAGCAGCTGCTGAGCCTGGGCACGCCCTCCGAGTACCACTAC
CTGACCATGGGGAACTGCACTTCCTGTGAGGGGCTCAACGACGCCAAGGACTACGCCCAC
ATCCGCTCGGCCATGAAGATCCTCCAGTTCTCCGACTCCGAGAGCTGGGACGTCATCAAG
CTGCTGGCTGCCATTCTCCACCTGGGGAATGTGGGGTTCATGGCTTCGGTCTTCGAGAAC
CTGGACGCCTCAGACGTGATGGAGACGCCCGCCTTTCCCACCGTGATGAAGTTACTGGAG
GTGCAGCACCAGGAGCTCCGGGACTGTCTGATCAAGCACACCATCCTCATCCGAGGGGAA
TTTGTCACCAGGTCCCTGAACATTGCCCAGGCTGCTGACCGGAGGGACGCCTTTGTCAAG
GGCATCTATGGGCACCTCTTCCTGTGGATTGTCAAGAAGATCAATGCCGCCATCTTCACA
CCACCAGCCCAGGACCCCAAAAATGTGCGGAGGGCCATCGGCCTCCTGGACATATTTGGC
TTTGAAAATTTCGAGAACAATAGCTTCGAGCAGCTCTGCATCAACTTCGCCAACGAGCAC
CTGCAGCAGTTCTTTGTGCAGCACGTGTTCACCATGGAGCAAGAGGAGTACCGCTCGGAG
AACATCTCCTGGGACTATATCCACTACACCGACAATCGGCCCACCCTGGACCTGCTGGCC
CTCAAGCCCATGAGCATCATCTCCCTCCTGGACGAAGAAAGCCGCTTCCCGCAGGGGACA
GATCTCACCATGCTGCAAAAGCTGAACAGCGTCCATGCCAACAACAAGGCCTTCCTACAG
CCCAAGAACATCCACGATGCCAGATTTGGCATTGCCCATTTTGCCGGCGAGGTGTACTAC
CAAGCAGAAGGCTTCCTGGAGAAGAACCGAGACGTGCTGAGCACAGATATCCTCACCCTG
GTTTACTCCTCCAAAAACAAGTTTCTGAGGGAGATATTCAACTTGGAGTTAGCAGAGACC
AAGCTGGGCCATGGGACCATCCGCCAGGCAAAGGCAGGAAACCATCTCTTCAAGTCTGCA
GACTCAAATAAACGGCCCTCCACCTTAGGAAGCCAGTTCAAACAGTCTCTGGACCAGCTG
ATGAAAATCCTGACCAACTGCCAGCCTTACTTCATCCGCTGCATCAAACCTAATGAGTAC
AAGAAGCCGCTGCTGTTCGACCGGGAGCTGTGCCTGCGGCAGCTGCGATACTCGGGCATG
ATGGAGACCGTGCACATCCGCAAGTCGGGCTTCCCCATCCGCTACACGTTCGAGGAGTTC
TCGCAGAGGTTCGGCGTGTTGCTGCCCAACGCCATGCGGATGCAGCTGCAAGGCAAGCTC
CGCCAGATGACCCTGGGCATCACTGACGTGTGGCTGCGGACAGACAAAGACTGGAAAGCG
GGGAAGACAAAAATTTTCCTGAGGGATCATCAGGACACTCTGCTGGAGGTACAGAGAAGC
CAGGTGCTAGACAGAGCGGCGCTCAGCATCCAGAAAGTCCTTCGGGGCTACAGATACAGG
AAGGAGTTCCTGAGGCAGAGGCGGGCAGCTGTGACCCTGCAGGCCTGGTGGAGAGGCTAC
TGCAACAGGAGGAATTTCAAGCTGATCCTCGTGGGCTTTGAGCGCCTGCAGGCTATTGCC
CGGAGCCAGCCGCTGGCGAGGCAGTACCAGGCCATGCGGCAGAGGACAGTCCAGCTGCAG
GCCCTGTGCAGGGGATACCTGGTGCGCCAGCAAGTCCAGGCCAAGAGGAGGGCAGTGGTG
GTCATTCAGGCCCATGCCAGGGGCATGGCTGCCCGGCGCAACTTCCAGCAAAGGAAGGCC
AATGCGCCGCTGGTCATCCCGGCCGAGGGGCAGAAAAGCCAAGGCGCTCTCCCTGCCAAG
AAGCGCAGATCCATCTACGACACCGTCACTGACACGGAGATGGTGGAGAAGGTGTTCGGC
TTCCTCCCTGCCATGATTGGGGGCCAGGAGGGCCAGGCCTCGCCGCACTTTGAGGATCTG
GAATCGAAGACCCAGAAGCTGCTTGAGGTTGACCTGGACACAGTCCCCATGGCGGAGGAG
CCTGAGGAGGATGTGGATGGCCTGGCCGAGTACACCTTCCCCAAGTTTGCTGTGACTTAC
TTCCAGAAATCAGCCAGCCACACACACATCCGGCGGCCCCTCCGATACCCGTTGCTTTAC
CACGAAGATGACACTGACTGCTTGGCCGCCCTGGTCATATGGAACGTCATCCTGAGGTTC
ATGGGTGATCTCCCAGAGCCAGTGCTGTATGCCAGGAGCAGCCAGCAGGGCAGCTCAGTG
ATGCGGCAGATCCATGACACGCTGGGCAGGGAGCACGGTGCCCAGGTTCCACAGCACAGT
AGATCTGCACAGGTGGCCAGCCAGCTGAACATTGGAGAGGAGGCATTGGAGCCTGATGGC
CTTGGTGCAGACCGGCCCATGTCCAACCTGGAGAAGGTGCACTTCATCGTGGGCTACGCC
ATCCTGCGGCCCAGCCTCAGGGATGAGATTTACTGCCAGATCTGCAAGCAGCTCTCGGAG
AACTTCAAAACAAGCAGCCTGGCCCGGGGCTGGATCCTGCTCAGCCTCTGCCTCGGCTGC
TTCCCACCCTCAGAGAGGTTCATGAAGTATCTACTGAACTTCATCGGCCAAGGGCCGGCG
ACCTACGGCCCCTTCTGTGCCGAGCGCCTGAGACGCACCTATGCCAATGGGGTGCGTGCG
GAGCCCCCCACCTGGCTGGAGCTGCAGGCTGTCAAGTCCAAGAAGCACATCCCCATCCAA
GTCATCTTGGCCACTGGAGAGAGCCTAACCGTCCCCGTGGACTCAGCCTCCACATCTCGG
GAAATGTGCATGCACATCGCTCACAAGCAGGGCCTCAGCGACCACCTGGGCTTCTCCCTC
CAGGTCGCCGTGTACGACAAGTTCTGGTCCCTGGGCAGCGGGCGCGACCACATGATGGAT
GCCATCGCCCGGTGTGAGCAGATGGCCCAGGAGAGGGGCGAGAGCCAGCGCCAGTCACCC
TGGCGCATCTACTTCCGGAAGGAATTCTTCACCCCCTGGCACGACTCCCGGGAGGACCCT
GTCAGCACCGAGCTTATTTACCGCCAAGTCCTCCGAGGAGTCTGGTCTGGCGAGTACAGC
TTCGAGAAGGAGGAAGAGCTGGTTGAGCTGCTGGCCCGGCACTGCTACGTGCAGCTCGGC
GCCTCAGCAGAGAGCAAGGCTGTCCAGGAGCTGCTGCCCAGCTGCATCCCCCACAAGCTG
TACAGGACCAAGCCCCCAGACAGGTGGGCGAGCCTCGTCACTGCCGCCTGCGCCAAGGCC
CCATACACTCAGAAGCAAGTCACACCACTGGCCGTGCGAGAGCAGGTGGTGGACGCCGCC
CGCCTGCAGTGGCCGCTGCTCTTCTCCCGGCTCTTCGAAGTCATCACACTCTCAGGCCCC
CGCCTGCCCAAGACGCAGCTGATCTTGGCTGTTAACTGGAAGGGGCTTTGCTTCCTGGAC
CAGCAGGAGAAGATGCTGCTGGAACTCTCTTTCCCAGAGGTCATGGGTCTGGCCACCAAC
AGGGAGGCCCAGGGCGGGCAGAGGCTGCTGCTCTCCACGATGCATGAGGAGTACGAGTTT
GTGTCACCCAGCAGTGTGGCCATCGCTGAGCTGGTGGCCCTGTTCCTGGAGGGCCTGAAG
GAGAGGTCCATTTTCGCCATGGCCCTGCAGGACAGGAAGGCCACAGATGACACCACCCTC
CTGGCCTTCAAGAAGGGGGACCTGTTGGTCCTCACAAAGAAGCAGGGGCTGCTGGCCTCT
GAGAACTGGACCCTCGGCCAGAACGACAGGACAGGCAAGACGGGGCTGGTGCCCATGGCC
TGCCTCTACACCATCCCCACGGTCACTAAGCCCTCGGCACAGCTGCTGAGCTTGCTTGCC
ATGTCACCAGAGAAGAGGAAGCTGGCGGCTCAGGAGGGGCAGTTCACAGAGCCACGTCCT
GAGGAGCCACCCAAGGAAAAGCTGCACACCCTGGAGGAGTTCTCCTATGAGTTCTTCAGG
GCTCCAGAGAAGGACATGGTGAGCATGGCCGTGCTGCCCCTGGCCCGTGCCCGTGGCCAC
CTGTGGGCCTATTCCTGCGAGCCGCTGCGACAGCCGCTGCTCAAGCGAGTCCACGCCAAC
GTCGACCTCTGGGACATCGCCTGCCAGATCTTTGTCGCCATCCTCCGGTACATGGGCGAC
TACCCTTCTCGGCAGGCCTGGCCCACCCTGGAGCTCACCGACCAGATCTTCACACTGGCC
CTGCAGCACCCGGCCCTCCAGGACGAGGTCTACTGCCAGATCCTGAAGCAGCTGACGCAC
AACTCCAACAGGCACAGCGAAGAGCGGGGCTGGCAGCTGCTGTGGCTGTGCACGGGCCTC
TTCCCGCCCAGCAAGGGGCTGCTGCCCCATGCCCAGAAGTTTATAGACACTCGGAGGGGG
AAGCTGCTGGCCCCCGACTGCAGCCGCCGAATCCAGAAGGTCCTGAGGACGGGGCCCCGG
AAGCAGCCCCCGCACCAGGTGGAGGTGGAGGCCGCAGAGCAGAACGTCTCCCGCATCTGC
CACAAGATCTACTTCCCCAATGACACCAGTGAGATGCTGGAGGTGGTTGCCAACACACGG
GTGCGGGATGTGTGTGACAGCATTGCCACCAGGCTGCAGCTGGCCTCCTGGGAGGGCTGC
AGCCTCTTCATCAAGATTTCAGACAAGGTCATCAGCCAGAAGGAGGGAGACTTCTTCTTT
GATTCCTTGAGGGAGGTGTCTGACTGGGTGAAGAAGAACAAGCCCCAGAAAGAAGGGGCC
CCCGTGACGCTCCCCTACCAGGTGTACTTCATGCGGAAATTGTGGCTCAACATATCTCCA
GGGAAGGATGTGAATGCAGACACCATACTCCATTACCACCAGGAGCTGCCCAAGTACCTG
CGCGGATTCCACAAGTGTTCGCGGGAGGATGCCATCCACCTGGCGGGCCTCATCTACAAG
GCCCAGTTCAACAACGACCGGTCCCAGCTGGCTAGTGTCCCCAAGATCCTGAGGGAACTG
GTGCCTGAGAACCTCACACGCCTGATGTCCTCGGAGGAGTGGAAAAAGAGCATCCTTCTA
GCCTATGACAAGCATAAGGACAAGACAGTGGAGGAGGCCAAGGTGGCCTTCCTGAAGTGG
ATCTGCCGGTGGCCCACCTTCGGATCCGCCTTCTTCGAGGTGAAGCAAACCTCGGAGCCT
TCCTACCCGGACGTCATCCTCATCGCCATCAACCGACATGGGGTTCTGCTCATCCACCCC
AAGACCAAGGACCTGCTCACCACCTATCCCTTCACCAAGATCTCCAGCTGGAGCAGCGGC
AGCACCTACTTCCACATGGCGCTGGGGAGCCTGGGCCGTGGCAGCCGCCTGCTGTGCGAG
ACCTCCCTGGGCTATAAGATGGATGACCTGCTGACCTCATATGTGCAGCAGCTCCTGAGT
GCCATGAACAAGCAGCGGGGCTCCAAGGCCCCAGCCCTGGCCAGCACCTTG
Protein sequence predicted based on nucleotide sequence
MSGFRLGDHVWLEPPSTHKTGVAIGGIIKEAKPGKVLVEDDEGKEHWIRAEDFGVLSPMH PNSVQGVDDMIRLGDLNEAGMVHNLLIRYQQHKIYTYTGSILVAVNPFQVLPLYTLEQVQ LYYSRHMGELPPHVFAIANNCYFSMKRNKRDQCCIISGESGAGKTETTKLILQFLATISG QHSWIEQQVLEANPILEAFGNAKTIRNDNSSRFGKYIDIYFNPSGVIEGARIEQFLLEKS RVCRQAPEERNYHIFYCMLMGVSAEDKQLLSLGTPSEYHYLTMGNCTSCEGLNDAKDYAH IRSAMKILQFSDSESWDVIKLLAAILHLGNVGFMASVFENLDASDVMETPAFPTVMKLLE VQHQELRDCLIKHTILIRGEFVTRSLNIAQAADRRDAFVKGIYGHLFLWIVKKINAAIFT PPAQDPKNVRRAIGLLDIFGFENFENNSFEQLCINFANEHLQQFFVQHVFTMEQEEYRSE NISWDYIHYTDNRPTLDLLALKPMSIISLLDEESRFPQGTDLTMLQKLNSVHANNKAFLQ PKNIHDARFGIAHFAGEVYYQAEGFLEKNRDVLSTDILTLVYSSKNKFLREIFNLELAET KLGHGTIRQAKAGNHLFKSADSNKRPSTLGSQFKQSLDQLMKILTNCQPYFIRCIKPNEY KKPLLFDRELCLRQLRYSGMMETVHIRKSGFPIRYTFEEFSQRFGVLLPNAMRMQLQGKL RQMTLGITDVWLRTDKDWKAGKTKIFLRDHQDTLLEVQRSQVLDRAALSIQKVLRGYRYR KEFLRQRRAAVTLQAWWRGYCNRRNFKLILVGFERLQAIARSQPLARQYQAMRQRTVQLQ ALCRGYLVRQQVQAKRRAVVVIQAHARGMAARRNFQQRKANAPLVIPAEGQKSQGALPAK KRRSIYDTVTDTEMVEKVFGFLPAMIGGQEGQASPHFEDLESKTQKLLEVDLDTVPMAEE PEEDVDGLAEYTFPKFAVTYFQKSASHTHIRRPLRYPLLYHEDDTDCLAALVIWNVILRF MGDLPEPVLYARSSQQGSSVMRQIHDTLGREHGAQVPQHSRSAQVASQLNIGEEALEPDG LGADRPMSNLEKVHFIVGYAILRPSLRDEIYCQICKQLSENFKTSSLARGWILLSLCLGC FPPSERFMKYLLNFIGQGPATYGPFCAERLRRTYANGVRAEPPTWLELQAVKSKKHIPIQ VILATGESLTVPVDSASTSREMCMHIAHKQGLSDHLGFSLQVAVYDKFWSLGSGRDHMMD AIARCEQMAQERGESQRQSPWRIYFRKEFFTPWHDSREDPVSTELIYRQVLRGVWSGEYS FEKEEELVELLARHCYVQLGASAESKAVQELLPSCIPHKLYRTKPPDRWASLVTAACAKA PYTQKQVTPLAVREQVVDAARLQWPLLFSRLFEVITLSGPRLPKTQLILAVNWKGLCFLD QQEKMLLELSFPEVMGLATNREAQGGQRLLLSTMHEEYEFVSPSSVAIAELVALFLEGLK ERSIFAMALQDRKATDDTTLLAFKKGDLLVLTKKQGLLASENWTLGQNDRTGKTGLVPMA CLYTIPTVTKPSAQLLSLLAMSPEKRKLAAQEGQFTEPRPEEPPKEKLHTLEEFSYEFFR APEKDMVSMAVLPLARARGHLWAYSCEPLRQPLLKRVHANVDLWDIACQIFVAILRYMGD YPSRQAWPTLELTDQIFTLALQHPALQDEVYCQILKQLTHNSNRHSEERGWQLLWLCTGL FPPSKGLLPHAQKFIDTRRGKLLAPDCSRRIQKVLRTGPRKQPPHQVEVEAAEQNVSRIC HKIYFPNDTSEMLEVVANTRVRDVCDSIATRLQLASWEGCSLFIKISDKVISQKEGDFFF DSLREVSDWVKKNKPQKEGAPVTLPYQVYFMRKLWLNISPGKDVNADTILHYHQELPKYL RGFHKCSREDAIHLAGLIYKAQFNNDRSQLASVPKILRELVPENLTRLMSSEEWKKSILL AYDKHKDKTVEEAKVAFLKWICRWPTFGSAFFEVKQTSEPSYPDVILIAINRHGVLLIHP KTKDLLTTYPFTKISSWSSGSTYFHMALGSLGRGSRLLCETSLGYKMDDLLTSYVQQLLS AMNKQRGSKAPALASTL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
