Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878168
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4632nts
Open reading frame: 1 to 4632
ATGAATAAGATGGCCTTGGCTTCCTTTATGAAAGGAAGAACAGTCATTGGGACACCAGAT
GAAGAGACCATGGATATAGAACTTCCAAAAAAATACCATGAAATGGTGGGAGTTATATTT
AGTGATACTTTCTCATATCGCCTGAAGTTTAATTGGGGATATAGAATCCCAGTTATAAAG
GAGCACTCTGAATACACAGAACACTGTTGGGCCATGCATGGTGAAATTTTTTGTTACTTG
GCAAAGTACTGGCTAAAAGGGTTTGTAGCTTTTCAAGCTGCAATTAATGCTGCAATTATA
GAAGTCACAACAAATCATTCTGTAATGGAGGAGTTGACATCAGTTATTGGAATAAATATG
AAGATACCACCTTTCATTTCTAAGGGAGAAATTATGAATGAATGGTTTCATTTTACTTGC
TTAGTTTCTTTCTCTTCTTTTATATACTTTGCATCATTAAATGTTGCAAGGGAAAGAGGA
AAATTTAAGAAACTGATGACAGTGATGGGTCTCCGAGAGTCAGCATTCTGGCTCTCCTGG
GGATTGACATACATTTGCTTCATCTTCATTATGTCCATTTTTATGGCTCTGGTCATAACA
TCAATCCCAATTGTATTTCATACTGGCTTCATGGTGATATTCACACTCTATAGCTTATAT
GGCCTTTCTTTGATAGCATTGGCTTTCCTCATGAGTGTTTTAATAAGGAAACCTATGCTC
GCTGGTTTGGCTGGATTTCTCTTCACTGTATTTTGGGGATGTCTGGGATTCACTGTGTTA
TATAGACAACTTCCTTTATCTTTGGGATGGGTATTAAGTCTTCTTAGCCCTTTTGCCTTC
ACTGCTGGAATGGCCCAGATTACACACCTGGATAATTACTTAAGTGGTGTTATTTTTCCT
GATCCCTCTGGGGATTCATACAAAATGATAGCCACTTTTTTCATTTTGGCATTTGATACT
CTTTTCTATTTGATATTCACATTATATTTTGAGCGAGTTTTACCTGATAAAGATGGCCAT
GGGGATTCTCCATTATTTTTCCTTAAGTCCTCATTTTGGTCCAAACATCAAAATACTCAT
CATGAAATCTTTGAGAATGAAATAAATCCTGAGCATTCCTCTGATGATTCTTTTGAACCG
GTGTCTCCAGAATTCCATGGAAAAGAAGCCATAAGAATCAGAAATGTTATAAAAGAATAT
AATGGAAAGACTGGAAAAGTAGAAGCATTGCAAGGCATATTTTTTGACATATATGAAGGA
CAGATCACTGCAATACTTGGGCATAATGGAGCTGGTAAATCAACACTGCTAAACATTCTT
AGTGGATTGTCTGTTTCTACAGAAGGATCAGCCACTATTTATAATACTCAACTCTCTGAA
ATAACTGACATGGAAGAAATTAGAAAGAATATTGGATTTTGTCCACAGTTCAATTTTCAA
TTTGACTTCCTCACTGTGAGAGAAAACCTCAGGGTATTTGCTAAAATAAAAGGGATTCAG
CCAAAGGAAGTGGAACAAGAGGTAAAAAGAATTATAATGGAATTAGACATGCAAAGCATT
CAAGACATTATTGCTAAAAAATTAAGTGGTGGGCAGAAGAGAAAACTAACACTAGGGATT
GCCATCTTAGGAGATCCTCAGGTTTTGCTGCTAGATGAACCAACTGCTGGATTGGATCCC
TTTTCAAGACACCGAGTGTGGAGCCTCCTGAAGGAGCATAAAGTAGACCGACTTATCCTC
TTCAGTACCCAATTCATGGATGAGGCTGACATCTTGGCTGATAGGAAAGTATTTCTGTCT
AATGGGAAGTTGAAATGTGCAGGATCATCTTTGTTTCTGAAGCGAAAGTGGGGTATTGGA
TATCATTTAAGTTTACACAGGAATGAAATGTGTGACACAGAAAAAATCACATCCCTTATT
AAGCAGCACATTCCTGATGCCAAGTTAACAACAGAAAGTGAAGAAAAACTTGTATATAGT
TTGCCTTTGGAAAAAACGAACAAATTTCCAGATCTTTACAGTGACCTTGATAAGTGTTCT
GACCAGGGCATAAGGAATTATGCTGTTTCAGTGACATCTCTGAATGAAGTATTCTTGAAC
CTAGAAGGAAAATCAGCAATTGATGAACCAGATTTTGACATTGGGAAACAAGAGAAAATA
CATGTGACAAGAAATACTGGAGATGAGTCTGAAATGGAACAGGTTCTTTGTTCTCTTCCT
GAAACAAGAAAGGCTGTCAGTAGTGCAGCTCTCTGGAGACGACAAATCTATGCAGTGGCA
ACACTTCGCTTCTTAAAGTTAAGGCGTGAAAGGAGAGCTCTTTTGTGTTTGTTACTAGTA
CTTGGAATTGCTTTTATCCCCATCATTCTAGAGAAGATAATGTATAAAGTAACTCGTGAA
ACTCATTGTTGGGAGTTTTCACCCAGTATGTATTTCCTTTCTCTGGAACAAATCCCGAAG
ACGCCTCTTACCAGCCTGTTAATCGTTAATAATACAGGATCAAATATTGAAGACCTCGTG
CATTCACTGAAGTGTCAGGATATAGTTTTGGAAATAGATGACTTTAGAAACAGAAATGGC
TCAGATGATCCCTCCTACAATGGAGCCATCATAGTGTCTGGTGACCAGAAGGATTACAGA
TTTTCTGTTGCGTGTAATACCAAGAAATTGAATTGTTTTCCTGTTCTTATGGGAATTGTT
AGCAATGCCCTTATGGGAATTTTTAACTTCACGGAGCTTATTCAAATGGAGAGCACTTCA
TTTTCTCGTGATGACATAGTGCTGGATCTTGGTTTTATAGATGGGTCCATATTTTTGTTG
TTGATCACAAACTGCGTTTCTCCTTTTATCGGCATGAGCAGCATCAGCGATTATAAAAAA
AATGTTCAATCCCAGTTATGGATTTCAGGCCTCTGGCCTTCAGCATACTGGTGTGGACAG
GCTCTGGTGGACATTCCATTATACTTCTTGATTCTCTTTTCAATACATTTAATTTACTAC
TTCATATTTCTGGGATTCCAGCTTTCATGGGAACTCATGTTTGTTTTGGTGGTATGCATA
ATTGGTTGTGCAGTTTCTCTTATATTCCTCACATATGTGCTTTCATTCATCTTTCGCAAG
TGGAGAAAAAATAATGGCTTTTGGTCTTTTGGCTTTTTTATTATCTTAATATGTGTATCC
ACAATTATGGTATCAACTCAATATGAAAAACTCAACTTAATTTTGTGCATGATTTTCATA
CCTTCCTTCACTTTGCTGGGGTATGTCATGTTATTGATCCAGCTCGACTTTATGAGAAAC
TTGGACAGTCTGGACAATAGAATAAATGAAGTCAATAAAACCATTCTTTTAACAACCTTA
ATACCATACCTTCAGAGTGTTATTTTCCTTTTTGTCATAAGGTGTCTGGAAATGAAGTAT
GGAAATGAAATAATGAATAAAGACCCAGTTTTCAGAATCTCTCCACGGAGTAGAGAAACT
CATCCCAATCCGGAAGAGCCCGAAGAAGAAGATGAAGATGTTCAAGCTGAAAGAGTCCAA
GCAGCAAATGCACTCACTGCTCCAAACTTGGAGGAGGAACCAGTCATAACTGCAAGCTGT
TTACACAAGGAATATTATGAGACAAAGAAAAGTTGCTTTTCAACAAGAAAGAAGAAAATA
GCCATCAGAAATGTTTCCTTTTGTGTTAAAAAAGGTGAAGTTTTGGGATTACTAGGACAC
AATGGAGCTGGTAAAAGTACTTCCATTAAAATGATAACTGGGTGCACAAAGCCAACTGCA
GGAGTGGTGGTGTTACAAGGCAGCAGAGCATCAGTAAGGCAACAGCATGACAACAGCCTC
AAGTTCTTGGGGTACTGCCCTCAGGAGAACTCACTGTGGCCCAAGCTTACAATGAAAGAG
CACTTGGAGTTGTATGCAGCTGTGAAAGGACTGGGCAAAGAAGATGCTGCTCTCAGTATT
TCACGATTGGTGGAAGCTCTTAAGCTCCAGGAACAACTTAAGGCTCCTGTGAAAACTCTA
TCAGAGGGAATAAAGAGAAAGCTGTGCTTTGTGCTGAGCATCCTGGGGAACCCATCAGTG
GTGCTTCTAGATGAGCCGTTCACCGGGATGGACCCCGAGGGGCAGCAGCAAATGTGGCAG
ATACTTCAGGCTACCGTTAAAAACAAGGAGAGGGGCACCCTCTTGACCACCCATTACATG
TCAGAGGCTGAGGCTGTGTGTGACCGTATGGCCATGATGGTGTCAGGAACGCTAAGGTGT
ATTGGTTCCATTCAACATCTGAAAAACAAGTTTGGTAGAGATTATTTACTAGAAATAAAA
ATGAAAGAACCTACCCAGGTGGAAGCTCTCCACACAGAGATTTTGAAGCTTTTCCCACAG
GCTGCTTGGCAGGAAAGATATTCCTCTTTAATGGCGTATAAGTTACCTGTGGAGGATGTC
CACCCTCTATCTCGGGCCTTTTTCAAGTTAGAGGCGATGAAACAGACCTTCAACCTGGAG
GAATACAGCCTCTCTCAGGCTACCTTGGAGCAGGTATTCTTAGAACTCTGTAAAGAGCAG
GAGCTGGGAAATGTTGATGATAAAATTGATACAACAGTTGAATGGAAACTTCTCCCACAG
GAAGACCCTTTG
Protein sequence predicted based on nucleotide sequence
MNKMALASFMKGRTVIGTPDEETMDIELPKKYHEMVGVIFSDTFSYRLKFNWGYRIPVIK EHSEYTEHCWAMHGEIFCYLAKYWLKGFVAFQAAINAAIIEVTTNHSVMEELTSVIGINM KIPPFISKGEIMNEWFHFTCLVSFSSFIYFASLNVARERGKFKKLMTVMGLRESAFWLSW GLTYICFIFIMSIFMALVITSIPIVFHTGFMVIFTLYSLYGLSLIALAFLMSVLIRKPML AGLAGFLFTVFWGCLGFTVLYRQLPLSLGWVLSLLSPFAFTAGMAQITHLDNYLSGVIFP DPSGDSYKMIATFFILAFDTLFYLIFTLYFERVLPDKDGHGDSPLFFLKSSFWSKHQNTH HEIFENEINPEHSSDDSFEPVSPEFHGKEAIRIRNVIKEYNGKTGKVEALQGIFFDIYEG QITAILGHNGAGKSTLLNILSGLSVSTEGSATIYNTQLSEITDMEEIRKNIGFCPQFNFQ FDFLTVRENLRVFAKIKGIQPKEVEQEVKRIIMELDMQSIQDIIAKKLSGGQKRKLTLGI AILGDPQVLLLDEPTAGLDPFSRHRVWSLLKEHKVDRLILFSTQFMDEADILADRKVFLS NGKLKCAGSSLFLKRKWGIGYHLSLHRNEMCDTEKITSLIKQHIPDAKLTTESEEKLVYS LPLEKTNKFPDLYSDLDKCSDQGIRNYAVSVTSLNEVFLNLEGKSAIDEPDFDIGKQEKI HVTRNTGDESEMEQVLCSLPETRKAVSSAALWRRQIYAVATLRFLKLRRERRALLCLLLV LGIAFIPIILEKIMYKVTRETHCWEFSPSMYFLSLEQIPKTPLTSLLIVNNTGSNIEDLV HSLKCQDIVLEIDDFRNRNGSDDPSYNGAIIVSGDQKDYRFSVACNTKKLNCFPVLMGIV SNALMGIFNFTELIQMESTSFSRDDIVLDLGFIDGSIFLLLITNCVSPFIGMSSISDYKK NVQSQLWISGLWPSAYWCGQALVDIPLYFLILFSIHLIYYFIFLGFQLSWELMFVLVVCI IGCAVSLIFLTYVLSFIFRKWRKNNGFWSFGFFIILICVSTIMVSTQYEKLNLILCMIFI PSFTLLGYVMLLIQLDFMRNLDSLDNRINEVNKTILLTTLIPYLQSVIFLFVIRCLEMKY GNEIMNKDPVFRISPRSRETHPNPEEPEEEDEDVQAERVQAANALTAPNLEEEPVITASC LHKEYYETKKSCFSTRKKKIAIRNVSFCVKKGEVLGLLGHNGAGKSTSIKMITGCTKPTA GVVVLQGSRASVRQQHDNSLKFLGYCPQENSLWPKLTMKEHLELYAAVKGLGKEDAALSI SRLVEALKLQEQLKAPVKTLSEGIKRKLCFVLSILGNPSVVLLDEPFTGMDPEGQQQMWQ ILQATVKNKERGTLLTTHYMSEAEAVCDRMAMMVSGTLRCIGSIQHLKNKFGRDYLLEIK MKEPTQVEALHTEILKLFPQAAWQERYSSLMAYKLPVEDVHPLSRAFFKLEAMKQTFNLE EYSLSQATLEQVFLELCKEQELGNVDDKIDTTVEWKLLPQEDPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
