Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878223
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5238nts
Open reading frame: 1 to 5238
ATGGGGAACCGCCGGGACCTGGGCCAGCCGCGGGCCGGTCTCTGCCTGCTCCTGGCCGCG
CTGCAGCTTCTGCCGGGGACGCAGGCCGATCCTGTGGATGTCCTGAAGGCCCTGGGTGTG
CAGGGAGGCCAGGCTGGGGTCCCCGAGGGGCCTGGCTTCTGTCCCCAGAGGACTCCAGAG
GGTGACCGGGCATTCAGAATTGGCCAGGCCAGCACGCTCGGCATCCCCACGTGGGAACTC
TTTCCAGAAGGCCACTTTCCTGAGAACTTCTCCTTGCTGATCACCTTGCGGGGACAGCCA
GCCAATCAGTCTGTCCTGCTGTCCATTTATGATGAAAGGGGTGCCCGGCAGTTGGGCCTG
GCACTGGGGCCAGCGCTGGGTCTCCTAGGTGACCCCTTCCGCCCCCTCCCCCAGCAGGTC
AACCTCACAGATGGCAGGTGGCACCGTGTGGCCGTCAGCATAGATGGTGAGATGGTGACC
CTGGTAGCTGACTGTGAAGCTCAGCCCCCTGTTTTGGGCCATGGCCCCCGCTTCATCAGC
ATAGCTGGACTCACTGTGCTGGGGACCCAGGACCTTGGGGAAAAGACTTTCGAGGGAGAC
ATTCAGGAGCTGCTGATAAGCCCAGATCCTCAGGCTGCCTTCCAGGCTTGTGAGCGGTAC
CTCCCCGACTGTGACAACCTGGCACCGGCAGCCACAGTGGCTCCCCAGGGTGAACCAGAA
ACCCCTCGTCCTCGGCGGAAGGGGAAGGGAAAAGGGAGGAAGAAAGGGCGAGGTCGCAAG
GGGAAGGGCAGGAAAAAGAACAAGGAAATTTGGACCTCAAGTCCACCTCCTGACTCCGCA
GAGAACCAGACCTCCACTGACATCCCCAAGACAGAGACTCCAGCTCCAAATCTGCCTCCG
ACCCCCACGCCTTTGGTCGTCACCTCCACTGTGACTACTGGACTCAATGCCACGATCCTA
GAGAGGAGCTTGGACCCTGACAGTGGAACCGAGCTGGGGACCCTGGAGACCAAGGCAGCC
AGGGAGGATGAAGAAGGAGATGATTCCACCATGGGCCCTGACTTCCGGGCAGCAGAATAT
CCATCTCGGACTCAGTTCCAGATCTTTCCTGGTGCTGGAGAGAAAGGAGCAAAAGGAGAG
CCCGCAGTGATTGAAAAGGGGCAGCAGTTTGAGGGACCTCCAGGAGCCCCAGGACCCCAA
GGGGTGGTTGGCCCCTCAGGCCCTCCCGGCCCCCCAGGATTCCCTGGCGACCCTGGTCCA
CCGGGCCCTGCTGGCCTCCCAGGAATCCCCGGCATTGATGGGATCCGAGGCCCACCGGGC
ACTGTGATCATGATGCCGTTCCAGTTTGCAGGCGGCTCCTTTAAAGGCCCCCCAGTCTCA
TTCCAGCAGGCCCAGGCTCAGGCAGTTCTGCAGCAGACTCAGCTCTCTATGAAAGGCCCC
CCTGGTCCAGTGGGGCTCACTGGGCGCCCAGGCCCTGTGGGTCTCCCCGGGCATCCAGGT
CTGAAAGGAGAGGAGGGAGCAGAAGGGCCACAGGGTCCCCGAGGCCTGCAGGGACCTCAT
GGACCCCCTGGCCGAGTGGGCAAGATGGGCCGCCCTGGAGCAGATGGAGCTCGGGGCCTC
CCAGGGGACACTGGACCTAAGGGTGATCGTGGCTTCGATGGCCTCCCTGGGCTGCCTGGT
GAGAAGGGCCAAAGGGGTGACTTTGGCCATGTGGGGCAACCCGGTCCCCCAGGAGAGGAT
GGTGAGAGGGGAGCAGAGGGACCTCCAGGGCCCACTGGCCAGGCTGGGGAGCCGGGTCCA
CGAGGACTGCTTGGCCCCAGAGGCTCTCCTGGCCCCACGGGTCGCCCGGGTGTGACTGGA
ATTGATGGTGCTCCTGGTGCCAAAGGCAATGTGGGTCCTCCAGGAGAACCAGGCCCTCCG
GGACAGCAGGGAAACCATGGGTCCCAGGGACTCCCCGGTCCCCAGGGACTCATTGGCACT
CCTGGGGAGAAGGGTCCCCCTGGAAACCCAGGAATTCCAGGCCTCCCAGGATCCGATGGC
CCTCTGGGTCACCCAGGACATGAGGGCCCCACGGGAGAGAAAGGGGCTCAGGGTCCACCA
GGGTCGGCAGGCCCTCCGGGCTATCCTGGACCTCGGGGAGTGAAGGGCACTTCAGGCAAC
CGGGGCCTCCAGGGGGAGAAAGGCGAGAAGGGAGAGGACGGCTTCCCAGGCTTCAAGGGC
GATGTGGGGCTCAAAGGTGATCAGGGGAAACCCGGAGCTCCAGGTCCCCGGGGAGAGGAT
GGTCCTGAGGGGCCGAAGGGGCAGGCGGGGCAGGCTGGCGAGGAGGGGCCCCCAGGCTCA
GCTGGGGAGAAGGGCAAGCTTGGGGTGCCAGGCCTCCCAGGTTATCCAGGACGCCCTGGA
CCTAAGGGATCTATTGGATTTCCCGGTCCCCTGGGACCCATAGGAGAGAAAGGGAAGTCG
GGAAAGACAGGGCAGCCAGGCCTGGAAGGAGAGCGGGGACCACCAGGTTCCCGTGGAGAG
AGGGGGCAACCGGGTGCCACAGGGCAACCAGGCCCCAAGGGCGATGTGGGCCAGGATGGA
GCCCCTGGGATCCCTGGAGAAAAGGGCCTCCCTGGTCTGCAAGGCCCTCCAGGATTCCCT
GGGCCAAAGGGCCCCCCTGGTCACCAAGGTAAAGATGGGCGACCAGGGCACCCTGGACAG
AGAGGAGAACTGGGCTTCCAAGGTCAGACAGGCCCGCCTGGACCAGCTGGTGTCTTAGGC
CCTCAGGGAAAGACAGGAGAAGTGGGACCTCTAGGTGAAAGGGGGCCTCCAGGCCCCCCT
GGACCTCCTGGTGAACAAGGTCTTCCTGGCCTGGAAGGCAGAGAGGGGGCCAAGGGGGAA
CTGGGACCACCAGGACCCCTTGGGAAAGAAGGGCCAGCTGGACTCAGGGGCTTTCCCGGC
CCCAAAGGGGGCCCTGGGGACCCGGGACCTACTGGCTTAAAGGGTGATAAGGGCCCCCCA
GGGCCCGTGGGGGCCAATGGCTCCCCTGGTGAGCGCGGTCCTTTGGGCCCAGCAGGAGGC
ATTGGACTTCCTGGCCAAAGTGGCAGCGAAGGCCCCGTTGGCCCTGCAGGCAAGAAGGGG
TCCCGGGGAGAACGTGGCCCCCCTGGCCCCACTGGCAAAGATGGGATCCCAGGGCCCCTG
GGGCCTCTGGGACCCCCTGGAGCTGCTGGGCCTTCTGGCGAGGAAGGGGACAAGGGGGAT
GTGGGTGCCCCCGGACACAAGGGGAGTAAAGGCGATAAAGGAGACGCGGGCCCACCTGGA
CAACCAGGGATACGGGGTCCTGCAGGACACCCAGGTCCCCCGGGAGCAGACGGGGCTCAG
GGGCGCCGGGGACCCCCAGGCCTCTTTGGGCAGAAAGGAGATGACGGAGTCAGAGGCTTT
GTGGGGGTGATTGGCCCTCCTGGACTGCAGGGGCTGCCAGGCCCTCCGGGAGAGAAAGGG
GAGGTCGGAGACGTCGGGTCCATGGGTCCCCATGGAGCTCCAGGTCCTCGGGGTCCCCAA
GGCCCCACTGGATCAGAGGGCACTCCAGGGCTGCCTGGAGGAGTTGGTCAGCCAGGCGCC
GTGGGTGAGAAGGGTGAGCGAGGGGACGCTGGAGACCCAGGGCCTCCAGGAGCCCCAGGC
ATCCCGGGGCCCAAGGGAGACATTGGTGAAAAGGGGGACTCAGGCCCATCTGGAGCTGCT
GGACCCCCAGGCAAGAAAGGTCCCCCTGGAGAGGATGGAGCCAAAGGGAGCGTGGGCCCC
ACGGGGCTGCCCGGAGATCTAGGGCCCCCAGGAGACCCTGGAGTTTCAGGCATAGATGGT
TCCCCAGGGGAGAAGGGAGACCCTGGTGATGTTGGGGGACCGGGTCCGCCTGGAGCTTCT
GGGGAGCCCGGCGCCCCCGGGCCCCCCGGCAAGAGGGGTCCTTCAGGCCACATGGGTCGA
GAAGGCAGAGAAGGGGAGAAAGGTGCCAAGGGGGAGCCAGGTCCTGATGGGCCCCCAGGG
AGGACGGGTCCAATGGGGGCTAGAGGGCCCCCTGGACGTGTGGGGCCTGAGGGTCTTCGA
GGGATCCCTGGCCCTGTGGGTGAACCAGGCCTCCTGGGAGCCCCTGGACAGATGGGCCCT
CCTGGCCCCCTGGGGCCCTCTGGCCTCCCAGGGCTGAAGGGAGACACTGGCCCCAAGGGG
GAAAAGGGCCACATTGGATTGATCGGTCTCATTGGCCCCCCGGGAGAAGCTGGTGAGAAA
GGAGATCAGGGGTTGCCAGGCGTGCAGGGACCCCCTGGTCCCAAGGGAGACCCTGGTCCC
CCTGGTCCCATTGGCTCTCTGGGCCACCCTGGGCCCCCAGGTGTGGCGGGCCCTCTAGGA
CAGAAAGGCTCAAAAGGGTCTCCGGGGTCCATGGGCCCCCGTGGAGACACTGGACCTGCA
GGCCCACCAGGCCCCCCGGGTGCCCCTGCCGAGCTGCATGGGCTGCGCAGGCGCCGGCGC
TTCGTCCCAGTCCCGCTTCCAGTCGTGGAGGGCGGCCTGGAGGAGGTGCTGGCCTCGCTC
ACATCGCTGAGCTTGGAGCTGGAGCAGCTGCGGCGTCCTCCCGGCACTGCGGAGCGCCCG
GGCCTCGTGTGCCACGAGCTGCACCGCAACCACCCGCACCTGCCTGATGGGGAATACTGG
ATTGACCCCAACCAGGGCTGCGCGCGGGACTCGTTCAGGGTTTTTTGCAACTTCACGGCG
GGAGGAGAGACCTGCCTCTATCCCGACAAGAAGTTTGAGATCGTGAAATTGGCCTCCTGG
TCCAAGGAAAAGCCTGGAGGCTGGTATAGCACATTCCGTCGAGGGAAGAAGTTCTCCTAC
GTGGACGCCGACGGGTCCCCAGTGAATGTCGTGCAGCTGAACTTCCTGAAACTGCTGAGT
GCCACAGCTCGCCAGAACTTCACCTACTCCTGCCAGAATGCAGCTGCCTGGCTGGACGAA
GCCACGGGTGACTACAGCCACTCCGCCCGCTTCCTTGGCACCAATGGAGAGGAGCTGTCT
TTCAACCAGACGACAGCAGCCACTGTCAGCGTCCCCCAGGATGGCTGCCGGCTCCGGAAA
GGACAGACGAAGACCCTTTTCGAATTCAGCTCTTCTCGAGCGGGATTTCTGCCCCTGTGG
GATGTGGCGGCCACTGACTTTGGCCAGACGAACCAAAAGTTTGGGTTTGAACTGGGCCCC
GTCTGCTTCAGCAGCTTG
Protein sequence predicted based on nucleotide sequence
MGNRRDLGQPRAGLCLLLAALQLLPGTQADPVDVLKALGVQGGQAGVPEGPGFCPQRTPE GDRAFRIGQASTLGIPTWELFPEGHFPENFSLLITLRGQPANQSVLLSIYDERGARQLGL ALGPALGLLGDPFRPLPQQVNLTDGRWHRVAVSIDGEMVTLVADCEAQPPVLGHGPRFIS IAGLTVLGTQDLGEKTFEGDIQELLISPDPQAAFQACERYLPDCDNLAPAATVAPQGEPE TPRPRRKGKGKGRKKGRGRKGKGRKKNKEIWTSSPPPDSAENQTSTDIPKTETPAPNLPP TPTPLVVTSTVTTGLNATILERSLDPDSGTELGTLETKAAREDEEGDDSTMGPDFRAAEY PSRTQFQIFPGAGEKGAKGEPAVIEKGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDPGP PGPAGLPGIPGIDGIRGPPGTVIMMPFQFAGGSFKGPPVSFQQAQAQAVLQQTQLSMKGP PGPVGLTGRPGPVGLPGHPGLKGEEGAEGPQGPRGLQGPHGPPGRVGKMGRPGADGARGL PGDTGPKGDRGFDGLPGLPGEKGQRGDFGHVGQPGPPGEDGERGAEGPPGPTGQAGEPGP RGLLGPRGSPGPTGRPGVTGIDGAPGAKGNVGPPGEPGPPGQQGNHGSQGLPGPQGLIGT PGEKGPPGNPGIPGLPGSDGPLGHPGHEGPTGEKGAQGPPGSAGPPGYPGPRGVKGTSGN RGLQGEKGEKGEDGFPGFKGDVGLKGDQGKPGAPGPRGEDGPEGPKGQAGQAGEEGPPGS AGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPIGEKGKSGKTGQPGLEGERGPPGSRGE RGQPGATGQPGPKGDVGQDGAPGIPGEKGLPGLQGPPGFPGPKGPPGHQGKDGRPGHPGQ RGELGFQGQTGPPGPAGVLGPQGKTGEVGPLGERGPPGPPGPPGEQGLPGLEGREGAKGE LGPPGPLGKEGPAGLRGFPGPKGGPGDPGPTGLKGDKGPPGPVGANGSPGERGPLGPAGG IGLPGQSGSEGPVGPAGKKGSRGERGPPGPTGKDGIPGPLGPLGPPGAAGPSGEEGDKGD VGAPGHKGSKGDKGDAGPPGQPGIRGPAGHPGPPGADGAQGRRGPPGLFGQKGDDGVRGF VGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPTGSEGTPGLPGGVGQPGA VGEKGERGDAGDPGPPGAPGIPGPKGDIGEKGDSGPSGAAGPPGKKGPPGEDGAKGSVGP TGLPGDLGPPGDPGVSGIDGSPGEKGDPGDVGGPGPPGASGEPGAPGPPGKRGPSGHMGR EGREGEKGAKGEPGPDGPPGRTGPMGARGPPGRVGPEGLRGIPGPVGEPGLLGAPGQMGP PGPLGPSGLPGLKGDTGPKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGPKGDPGP PGPIGSLGHPGPPGVAGPLGQKGSKGSPGSMGPRGDTGPAGPPGPPGAPAELHGLRRRRR FVPVPLPVVEGGLEEVLASLTSLSLELEQLRRPPGTAERPGLVCHELHRNHPHLPDGEYW IDPNQGCARDSFRVFCNFTAGGETCLYPDKKFEIVKLASWSKEKPGGWYSTFRRGKKFSY VDADGSPVNVVQLNFLKLLSATARQNFTYSCQNAAAWLDEATGDYSHSARFLGTNGEELS FNQTTAATVSVPQDGCRLRKGQTKTLFEFSSSRAGFLPLWDVAATDFGQTNQKFGFELGP VCFSSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
