Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878235
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5685nts
Open reading frame: 1 to 5685
ATGAAAGCCATGCCCTGGAACTGGACCTGCCTTCTCTCCCACCTCCTCATGGTGGGCATG
GGCTCCTCCACTTTGCTCACCCGGCAGCCAGCCCCGCTGTCCCAGAAGCAGCGGTCATTT
GTCACATTCCGAGGAGAGCCCGCCGAGGGTTTCAATCACCTGGTGGTGGATGAGAGGACA
GGACACATTTACTTGGGGGCCGTCAATCGGATTTACAAGCTCTCCAGCGACCTGAAGGTC
TTGGTGACGCATGAGACAGGGCCGGACGAGGACAACCCCAAGTGTTACCCACCCCGCATC
GTCCAGACCTGCAATGAGCCCCTGACCACCACCAACAATGTCAACAAGATGCTCCTCATA
GACTACAAGGAGAACAGGCTGATTGCCTGTGGGAGCCTGTACCAAGGCATCTGCAAGCTG
CTGAGGCTGGAGGACCTCTTCAAGCTGGGGGAGCCTTATCATAAGAAGGAGCACTATCTG
TCAGGTGTCAACGAGAGCGGCTCAGTCTTTGGAGTGATCGTCTCCTACAGCAACCTGGAT
GACAAGCTGTTCATTGCCACGGCAGTGGATGGGAAGCCCGAGTATTTTCCCACCATCTCC
AGCCGGAAACTGACCAAGAACTCTGAGGCGGATGGCATGTTCGCGTACGTCTTCCATGAT
GAGTTCGTGGCCTCGATGATTAAGATCCCTTCGGACACCTTCACCATCATCCCTGACTTT
GATATCTACTATGTCTATGGTTTTAGCAGTGGCAACTTTGTCTACTTTTTGACCCTCCAA
CCTGAGATGGTGTCTCCACCAGGCTCCACCACCAAGGAGCAGGTGTATACATCCAAGCTC
GTGAGGCTTTGCAAGGAGGACACAGCCTTCAACTCCTATGTAGAGGTGCCCATTGGCTGT
GAGCGCAGTGGGGTGGAGTACCGCCTGCTGCAGGCTGCCTACCTGTCCAAAGCGGGGGCC
GTGCTTGGCAGGACCCTTGGAGTCCATCCAGATGATGACCTGCTCTTCACCGTCTTCTCC
AAGGGCCAGAAGCGGAAAATGAAATCCCTGGATGAGTCGGCCCTGTGCATCTTCATCTTG
AAGCAGATAAATGACCGCATTAAGGAGCGGCTGCAGTCTTGTTACCGGGGCGAGGGCACG
CTGGACCTGGCCTGGCTCAAGGTGAAGGACATCCCCTGCAGCAGTGCGCTCTTAACCATT
GACGATAACTTCTGTGGCCTGGACATGAATGCTCCCCTGGGAGTGTCCGACATGGTGCGT
GGAATTCCCGTCTTCACGGAGGACAGGGACCGCATGACGTCTGTCATCGCATATGTCTAC
AAGAACCACTCTCTGGCCTTTGTGGGCACCAAAAGTGGCAAGCTGAAGAAGATCCGGGTG
GATGGACCCAGGGGCAACGCCCTCCAGTATGAGACGGTGCAGGTGGTGGACCCCGGCCCA
GTCCTCCGGGATATGGCCTTCTCCAAGGACCACGAGCAACTCTACATCATGTCAGAGAGG
CAGCTCACCAGAGTCCCTGTGGAGTCCTGTGGTCAGTATCAGAGCTGCGGCGAGTGCCTT
GGCTCAGGCGACCCCCACTGTGGCTGGTGTGTGCTGCACAACACTTGCACCCGGAAGGAG
CGGTGTGAGCGGTCCAAGGAGCCCCGCAGGTTTGCCTCGGAGATGAAGCAGTGTGTCCGG
CTGACGGTCCATCCCAACAATATCTCCGTCTCTCAGTACAACGTGCTGCTGGTCCTGGAG
ACGTACAATGTCCCGGAGCTGTCAGCTGGCGTCAACTGCACCTTTGAGGACCTGTCAGAG
ATGGATGGGCTGGTCGTGGGCAATCAGATCCAGTGCTACTCCCCTGCAGCCAAGGAGGTG
CCCCGGATCATCACAGAGAATGGGGACCACCATGTCGTACAGCTTCAGCTCAAATCAAAG
GAGACCGGCATGACCTTCGCCAGCACCAGCTTTGTCTTCTACAATTGCAGCGTCCACAAT
TCGTGCCTGTCCTGCGTGGAGAGTCCATACCGCTGCCACTGGTGTAAATACCGGCATGTC
TGCACCCATGACCCCAAGACCTGCTCCTTCCAGGAAGGCCGAGTGAAGCTGCCCGAGGAC
TGCCCCCAGCTGCTGCGAGTGGACAAGATCCTGGTGCCCGTGGAGGTGATCAAGCCTATC
ACGCTGAAGGCCAAGAACCTCCCCCAGCCCCAGTCTGGGCAGCGTGGCTACGAATGCATC
CTCAACATTCAGGGCAGCGAGCAGCGAGTGCCCGCCCTGCGCTTCAACAGCTCCAGCGTA
CAGTGCCAGAACACCTCTTATTCCTATGAAGGGATGGAGATCAACAACCTGCCCGTGGAG
TTGACAGTCGTGTGGAATGGGCACTTCAACATTGACAACCCAGCTCAGAATAAAGTTCAC
CTCTACAAGTGTGGAGCCATGCGTGAGAGCTGCGGGCTGTGCCTCAAGGCTGACCCAGAC
TTCGCATGTGGCTGGTGCCAGGGCCCAGGCCAGTGCACCCTGCGCCAGCACTGCCCTGCC
CAGGAGAGCCAGTGGCTGGAGCTGTCTGGTGCCAAAAGCAAGTGCACAAACCCCCGCATC
ACAGAGATAATCCCGGTGACAGGCCCCCGGGAAGGGGGCACCAAGGTCACTATCCGAGGG
GAGAACCTGGGCCTGGAATTTCGCGACATCGCCTCCCATGTCAAGGTTGCTGGCGTGGAG
TGCAGCCCTTTAGTGGATGGTTACATCCCTGCAGAACAGATCGTGTGTGAGATGGGGGAG
GCCAAGCCCAGCCAGCATGCAGGCTTCGTGGAGATCTGCGTGGCTGTGTGTCGGCCTGAA
TTCATGGCCCGGTCCTCACAGCTCTATTACTTCATGACACTGACTCTCTCAGATCTGAAG
CCCAGCCGGGGGCCCATGTCCGGAGGGACCCAAGTGACCATCACAGGCACCAACCTGAAT
GCCGGAAGCAACGTGGTGGTGATGTTTGGAAAGCAGCCCTGTCTCTTCCACAGGCGATCT
CCATCCTACATTGTCTGCAACACCACATCCTCAGATGAGGTGCTAGAGATGAAGGTGTCG
GTGCAGGTGGACAGGGCCAAGATCCACCAGGACCTGGTCTTTCAGTATGTGGAAGACCCC
ACCATCGTGCGGATTGAGCCAGAATGGAGCATTGTCAGTGGAAACACACCCATCGCCGTA
TGGGGGACCCACCTGGACCTCATACAGAACCCCCAGATCCGTGCCAAGCATGGAGGGAAG
GAGCACATCAATATCTGTGAGGTTCTGAACGCTACTGAGATGACCTGTCAGGCGCCCGCC
CTCGCTCTGGGTCCTGACCACCAGTCAGACCTGACCGAGAGGCCCGAGGAGTTTGGCTTC
ATCCTGGACAACGTCCAGTCCCTGCTCATCCTCAACAAGACCAACTTCACCTACTATCCC
AACCCGGTGTTTGAGGCCTTTGGTCCCTCAGGAATCCTGGAGCTCAAGCCTGGCACGCCC
ATCATCCTAAAGGGCAAGAACCTGATCCCGCCTGTGGCTGGGGGCAACGTGAAGCTGAAC
TACACTGTGCTGGTTGGGGAGAAGCCGTGCACCGTGACCGTGTCAGATGTCCAGCTGCTC
TGCGAGTCCCCCAACCTCATCGGCAGGCACAAAGTGATGGCCCGTGTCGGTGGCATGGAG
TACTCCCCGGGGATGGTGTACATTGCCCCGGACAGCCCGCTCAGCCTGCCCGCCATCGTC
AGCATCGCAGTGGCTGGCGGCCTCCTCATCATTTTCATCGTGGCCGTGCTCATTGCCTAT
AAACGCAAGTCCCGCGAAAGTGACCTCACGCTGAAGCGGCTGCAGATGCAGATGGACAAC
CTGGAGTCCCGTGTGGCCCTGGAGTGCAAGGAAGCCTTTGCCGAGCTGCAGACGGACATC
CATGAGCTGACCAGTGACCTGGATGGAGCCGGGATTCCGTTCCTGGACTATAGAACTTAC
ACCATGCGGGTGCTGTTCCCAGGAATTGAAGACCACCCTGTCCTCCGGGACCTTGAGGTC
CCGGGCTACCGGCAGGAGCGTGTGGAGAAAGGCCTGAAGCTCTTCGCCCAGCTCATCAAC
AACAAGGTGTTCCTGCTGTCCTTCATCCGCACGCTTGAGTCCCAGCGTAGCTTCTCCATG
CGCGACCGTGGCAACGTGGCCTCACTCATCATGACCGTGCTGCAGAGCAAGCTGGAGTAC
GCCACTGATGTGCTGAAGCAGCTGCTGGCCGACCTCATTGACAAGAACCTGGAGAGCAAG
AACCACCCTAAGCTGCTGCTCAGGAGGACTGAGTCAGTGGCTGAGAAGATGCTGACCAAT
TGGTTTACTTTCCTCCTCTACAAGTTCCTCAAGGAGTGTGCTGGGGAGCCCCTCTTCTCC
CTGTTCTGTGCCATCAAGCAGCAGATGGAGAAGGGCCCCATTGACGCCATCACGGGCGAG
GCCCGCTACTCCTTGAGCGAGGACAAGCTCATCCGCCAGCAGATTGACTACAAAACCCTG
GTCCTGAGCTGTGTCAGCCCAGACAATGCCAACAGCCCCGAGGTCCCAGTAAAGATCCTC
AACTGTGACACCATCACTCAGGTCAAGGAGAAGATTCTGGATGCCATCTTCAAGAATGTG
CCTTGCTCCCACCGGCCCAAAGCTGCAGATATGGATCTGGAGTGGCGACAAGGAAGTGGG
GCAAGGATGATCTTGCAGGATGAAGACATCACCACCAAGATTGAGAATGATTGGAAGCGA
CTGAACACACTGGCCCACTACCAGGTGCCAGATGGTTCCGTGGTGGCATTAGTGTCCAAG
CAGGTGACAGCCTATAACGCAGTGAACAACTCCACCGTCTCCAGGACCTCAGCAAGTAAA
TATGAAAACATGATCCGGTACACGGGCAGCCCCGACAGCCTCCGCTCACGGACACCTATG
ATCACTCCTGACCTGGAGAGTGGAGTCAAGATGTGGCACCTAGTGAAGAACCACGAGCAC
GGAGACCAGAAGGAGGGGGACCGGGGGAGCAAGATGGTGTCTGAAATCTACCTGACCCGA
CTCCTGGCCACTAAGGGCACACTGCAGAAGTTTGTGGATGACCTCTTTGAGACCATCTTC
AGCACGGCACACCGTGGCTCTGCCCTGCCCCTGGCCATCAAGTACATGTTTGACTTCCTG
GATGAGCAGGCTGATAAACATGGCATTCATGACCCGCACGTCCGCCATACCTGGAAGAGC
AATTGCCTGCCCCTGAGGTTTTGGGTCAACATGATCAAGAACCCGCAGTTTGTGTTTGAC
ATCCATAAGAACAGCATCACAGACGCCTGCCTCTCTGTGGTGGCTCAGACCTTCATGGAC
TCTTGCTCCACGTCAGAGCACCGGCTGGGCAAGGACTCGCCCTCCAACAAGCTGCTGTAT
GCCAAGGACATCCCCAGCTACAAGAATTGGGTGGAGAGGTATTACTCAGACATAGGGAAG
ATGCCAGCCATCAGCGACCAAGACATGAACGCATACCTGGCTGAGCAGTCCCGGATGCAC
ATGAATGAGTTCAACACCATGAGTGCACTCTCAGAGATCTTCTCCTATGTGGGCAAATAC
AGCGAGGAGATCCTTGGACCTCTGGACCACGATGACCAGTGTGGGAAGCAGAAACTGGCC
TACAAACTAGAACAAGTCATAACCCTCATGAGCTTAGACAGCTTG
Protein sequence predicted based on nucleotide sequence
MKAMPWNWTCLLSHLLMVGMGSSTLLTRQPAPLSQKQRSFVTFRGEPAEGFNHLVVDERT GHIYLGAVNRIYKLSSDLKVLVTHETGPDEDNPKCYPPRIVQTCNEPLTTTNNVNKMLLI DYKENRLIACGSLYQGICKLLRLEDLFKLGEPYHKKEHYLSGVNESGSVFGVIVSYSNLD DKLFIATAVDGKPEYFPTISSRKLTKNSEADGMFAYVFHDEFVASMIKIPSDTFTIIPDF DIYYVYGFSSGNFVYFLTLQPEMVSPPGSTTKEQVYTSKLVRLCKEDTAFNSYVEVPIGC ERSGVEYRLLQAAYLSKAGAVLGRTLGVHPDDDLLFTVFSKGQKRKMKSLDESALCIFIL KQINDRIKERLQSCYRGEGTLDLAWLKVKDIPCSSALLTIDDNFCGLDMNAPLGVSDMVR GIPVFTEDRDRMTSVIAYVYKNHSLAFVGTKSGKLKKIRVDGPRGNALQYETVQVVDPGP VLRDMAFSKDHEQLYIMSERQLTRVPVESCGQYQSCGECLGSGDPHCGWCVLHNTCTRKE RCERSKEPRRFASEMKQCVRLTVHPNNISVSQYNVLLVLETYNVPELSAGVNCTFEDLSE MDGLVVGNQIQCYSPAAKEVPRIITENGDHHVVQLQLKSKETGMTFASTSFVFYNCSVHN SCLSCVESPYRCHWCKYRHVCTHDPKTCSFQEGRVKLPEDCPQLLRVDKILVPVEVIKPI TLKAKNLPQPQSGQRGYECILNIQGSEQRVPALRFNSSSVQCQNTSYSYEGMEINNLPVE LTVVWNGHFNIDNPAQNKVHLYKCGAMRESCGLCLKADPDFACGWCQGPGQCTLRQHCPA QESQWLELSGAKSKCTNPRITEIIPVTGPREGGTKVTIRGENLGLEFRDIASHVKVAGVE CSPLVDGYIPAEQIVCEMGEAKPSQHAGFVEICVAVCRPEFMARSSQLYYFMTLTLSDLK PSRGPMSGGTQVTITGTNLNAGSNVVVMFGKQPCLFHRRSPSYIVCNTTSSDEVLEMKVS VQVDRAKIHQDLVFQYVEDPTIVRIEPEWSIVSGNTPIAVWGTHLDLIQNPQIRAKHGGK EHINICEVLNATEMTCQAPALALGPDHQSDLTERPEEFGFILDNVQSLLILNKTNFTYYP NPVFEAFGPSGILELKPGTPIILKGKNLIPPVAGGNVKLNYTVLVGEKPCTVTVSDVQLL CESPNLIGRHKVMARVGGMEYSPGMVYIAPDSPLSLPAIVSIAVAGGLLIIFIVAVLIAY KRKSRESDLTLKRLQMQMDNLESRVALECKEAFAELQTDIHELTSDLDGAGIPFLDYRTY TMRVLFPGIEDHPVLRDLEVPGYRQERVEKGLKLFAQLINNKVFLLSFIRTLESQRSFSM RDRGNVASLIMTVLQSKLEYATDVLKQLLADLIDKNLESKNHPKLLLRRTESVAEKMLTN WFTFLLYKFLKECAGEPLFSLFCAIKQQMEKGPIDAITGEARYSLSEDKLIRQQIDYKTL VLSCVSPDNANSPEVPVKILNCDTITQVKEKILDAIFKNVPCSHRPKAADMDLEWRQGSG ARMILQDEDITTKIENDWKRLNTLAHYQVPDGSVVALVSKQVTAYNAVNNSTVSRTSASK YENMIRYTGSPDSLRSRTPMITPDLESGVKMWHLVKNHEHGDQKEGDRGSKMVSEIYLTR LLATKGTLQKFVDDLFETIFSTAHRGSALPLAIKYMFDFLDEQADKHGIHDPHVRHTWKS NCLPLRFWVNMIKNPQFVFDIHKNSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLY AKDIPSYKNWVERYYSDIGKMPAISDQDMNAYLAEQSRMHMNEFNTMSALSEIFSYVGKY SEEILGPLDHDDQCGKQKLAYKLEQVITLMSLDSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
