Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878236
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6438nts
Open reading frame: 1 to 6438
ATGGCCGCCTTCGGGCTTCTCAGCTATGAGCAGAGACCGCTGAAGCGCCCCCGGCTCGGG
CCGCCCGACGTCTACCCACAGGACCCCAAGCAGAAGGAGGATGAACTTACTGCTGTGAAT
GTAAAGCAAGGCTTCAATAATCAGCCAGCCTTCACTGGAGATGAACATGGCTCAGCCAGA
AATATTGTAATTAACCCATCAAAGATTGGAGCTTATTTTAGCAGCATATTAGCTGAGAAA
CTGAAGCTTAACACTTTCCAGGACACGGGAAAGAAGAAACCACAAGTTAATGCTAAAGAT
AATTATTGGCTGGTTACTGCTCGATCCCAGAGTGCAATTCATAGTTGGTTTTCTGACTTA
GCAGGAAATAAGCCACTTTCTATTTTGGCAAAAAAGGTTCCTATCCTTAGTAAAAAAGAG
GATGTTTTTGCATATTTAGCTAAATATTCTGTGCCAATGGTTCGAGCAACGTGGCTGATC
AAGATGACTTGTGCCTATTATTCTGCTATATCTGAAGCTAAAATTAAGAAACGTCAGGCT
CCTGATCCGAATTTGGAGTGGACACAGATATCTACCAGATATCTTCGAGAGCAGTTGGCC
AAGATTTCTGACTTTTACCACATGGCCTCCAGCACGGGCGATGGCCCTGTCCCTGTGCCA
CCAGAGGTGGAGCAAGCCATGAAGCAATGGGAATACAACGAAAAGCTAGCATTTCACATG
TTCCAGGAAGGAATGTTAGAAAAACACGAATATTTGACATGGATCCTGGATGTTTTAGAA
AAGATCAGACCAATGGATGATGATCTTCTTAAACTCTTGCTACCACTAATGCTGCAGTAT
TCAGATGAGTTTGTTCAGTCGGCCTACCTGTCTCGTCGTCTTGCCTACTTTTGTGCCCGG
CGTCTTTCCTTGCTGCTGAGCGATAGCCCCAACCTCCTTGCTGCCCACTCACCCCACATG
ATGATAGGACCAAACAACTCGAGTATCGGGGCCCCCAGCCCTGGCCCCCCCGGCCCTGGC
ATGAGCCCCGTGCAGCTGGCCTTCTCAGATTTTCTTTCCTGTGCACAGCATGGTCCCCTG
GTTTATGGACTTAGTTGTATGTTGCAGACTGTCACTCTCTGTTGCCCAAGTGCCTTGGTG
TGGAATTATTCCACAAATGAAAATAAGAGCGCAAACCCAGGCTCACCCCTGGATCTGCTG
CAGGTGGCCCCGTCCAGCCTCCCCATGCCGGGTGGGAACACGGCTTTCAATCAGCAGGTT
CGGGCAAGGATTTATGAAGTAGAACAACAGATAAAACAAAGAGGCCGTGCAGTGGAAGTT
CGGTGGTCATTTGACAAGTGCCAAGAATCCACAGCAGGGGTGACTATTAGTCGGGTTTTG
CACACGTTGGAAGTTTTGGATCGTCACTGTTTTGACCGAACTGATTCCAGCAATTCCATG
GAGACACTTTATCATAAGATTTTCTGGGCAAACCAAAACAAAGATAACCAAGAGGTTGCG
CCCAACGATGAAGCTGTGGTGACGCTGTTATGTGAATGGGCCGTGAGCTGCAAACGGTCT
GGCAAGCACAGGGCCATGGCTGTGGCAAAACTCCTGGAGAAGAGGCAAGCAGAAATTGAG
GCAGAGAGATGTGGTGAATCAGAAGTCTTAGATGAGAAGGAGTCTATTTCTTCATCCTCT
CTTGCTGGATCCAGTTTGCCTGTTTTCCAGAATGTGCTGTTAAGGTTTTTAGATACACAG
GCCCCCTCTTTGTCGGACCCAAACAGTGAATGTGAAAAGGTGGAATTTGTGAACCTGGTG
CTGCTCTTCTGCGAGTTCATCCGCCATGATGTCTTCTCCCATGACGCATACATGTGTACC
CTCATATCTCGAGGAGATTTGTCAGTCACTGCCTCAACTCGGCCGCGGTCACCAGTAGGG
GAAAATGCAGATGAACACTATTCAAAGGACCATGATGTGAAAATGGAGATTTTTTCTCCT
ATGCCTGGAGAATCCTGTGAGAATGCCAACACTTCGTTGGGCAGAAGAATGTCAGTTAAT
TGTGAGAAGTTGGTGAAGAGGGAAAAGCCAAGGGAATTAATTTTTCCATCTAATTATGAC
CTCCTTCGCCACTTACAGTATGCAACACATTTTCCTATACCTCTGGATGAATCTTCAAGT
CATGAATGTAACCAGCGCACAATCCTTCTCTATGGAGTCGGCAAAGAGCGTGATGAAGCA
AGGCATCAGCTGAAGAAGATTACCAAAGATATCCTGAAAATTCTAAATAAGAAGAGCACC
ACAGAGACAGGGGTTGGGGACGAAGGACAAAAAGCCAGGAAGAACAAACAGGAGACATTT
CCAACACTGGAGACTGTGTTCACTAAACTCCAGCTCCTTTCATATTTTGATCAACATCAA
GTGACATCTCAGATTTCTAACAATGTGCTAGAACAAATCACAAGCTTTGCGTCAGGAACA
TCCTATCATCTCCCTTTGGCTCACCACATTCAGCTCATCTTTGATCTCATGGAGCCAGCA
CTGAACATCAACGGACTAATTGACTTCGCAATACAGTTACTAAATGAACTGAGTGTTGTG
GAAGCTGAACTGCTCCTAAAATCCTCCAGCCTGGCAGGAAGTTATACAACAGGACTGTGT
GTCTGCATCGTGGCTGTTCTCAGGCGCTATCACAGTTGTCTAATCTTGAATCCTGATCAG
ACAGCCCAGGTGTTTGAAGGGTTGTGTGGTGTGGTCAAGCATGTCGTAAACCCCTCAGAA
TGTTCTTCCCCTGAAAGATGCATTTTAGCCTACCTCTATGATCTCTATGTGTCATGTAGC
CACCTCAGAAGTAAATTTGGAGACCTCTTCAGTAGTGCCTGTTCAAAAGTAAAGCAAACC
ATATATAATAACGTGATGCCTGCAAATTCGAACTTGCGATGGGATCCAGACTTCATGATG
GATTTTATTGAGAATCCCTCAGCCCGCAGCATCAACTACTCAATGCTGGGCAAGATCCTC
AGTGACAATGCGGCCAATCGCTACAGCTTTGTCTGCAATACACTCATGAATGTATGTATG
GGCCATCAGGATGCTGGCAGGATTAACGACATAGCCAATTTCTCCTCTGAGCTTACGGCT
TGCTGCACTGTTCTTAGTTCAGAATGGCTGGGGGTTCTGAAGGCTCTTTGTTGTTCTTCA
AATCACGTGTGGGGGTTTAATGATGTACTTTGCACTGTAGATGTGAGTGACCTTTCATTC
CATGATTCATTAGCTACTTTCATTGCTATTCTGATAGCACGACAGTGTTTTTCCCTGGAG
GACGTCGTGCAGCATGTCGCACTTCCCTCTCTTCTAGCAGCAGCTTGTGGGGATGCGGAC
GCCGAGCCTGGGGCGAGAATGACATGCCGACTCTTGCTTCATCTCTTCCGAGCTCCCCAG
GCCTGCTTCTTACCTCAAGCAACGGGCAAACCTTTCCCTGGAATAAGATCATCTTGTGAT
AGACACCTCTTAGCCGCTGCTCACAACAGCATTGAAGTGGGAGCCGTGTTTGCTGTCTTA
AAAGCAATTATGATGCTTGGAGATGCCAAAATTGGCAATAACAGTGTCAGCTCTTTAAAG
AATGATGACTTCACCATGAGAGGTTTGCGATGTGATGGGAATGCTGATGATATCTGGACT
GCCTCACAAAATCCAAAATCCTGTGGGAAAAGCATTTCCATAGAAACTGCCAATTTAAGA
GAATACGCTAGATATGTACTGAGGACTATCTGTCAACAGGAATGGGTAGGAGAGCATTGC
TTAAAAGAACCTGAAAGATTATGTACAGACAAAGAACTTATATTGGACCCTGTGCTTTCA
AATATGCAAGCACAGAAATTACTGCAGCTTATCTGTTATCCTCATGGCATTAAAGAATGT
ACCGAGGGGGACAATCTGCAAAGACAGCACATTAAGCGTATTCTTCAGAATCTTGAGCAG
TGGACACTGAGGCAATCCTGGTTAGAACTCCAGCTAATGATCAAACAGTGCTTGAAGGAC
CCTGGCTCTGGTTCTGTGGCCGAAATGAACAACTTACTGGACAATATTGCAAAGGCAACA
ATAGAGGTATTCCAGCAGTCTGCAGACCTAAATAATTCTTCTAATTCTGGCATGAGCCTC
TTCAACCCAAACAGTATTGGAAGTGCTGATACAAGTAGCACGAGACAGAATGGAATAAAG
ACATTCCTAAGTTCCTCCGAACGCAGGGGTGTATGGTTGGTGGCCCCCCTCATCGCCAGG
TTGCCAACTTCTGTGCAAGGAAGAGTGCTGAAAGCCGCTGGGGAAGAGCTGGAGAAGGGA
CAGCACTTGGGTTCTTCTTCCAAAAAGGAAAGGGACAGACAGAAACAGAAAAGTATGTCT
CTTTTGAGTCAACAACCCTTCCTCTCACTGGTACTTACCTGCCTTAAGGGACAAGATGAA
CAAAGGGAAGGCCTCCTAACATCTCTCCAGAATCAAGTTAACCAGATTTTAAGTAACTGG
AGAGAAGAACGATACCAAGATGACATAAAAGCGCGGCAGATGATGCACGAAGCATTGCAA
CTCCGCCTAAATTTGGTAGGAGGAATGTTTGACACGGTGCAGAGGAGCACCCAGTGGACT
ACAGACTGGGCCCTGCTACTCCTTCAGATCATTACTTCAGGAACTGTTGACATGCACACT
AACAATGAATTATTCACAACAGTTCTTGACATGCTGGGTGTTTTAATCAATGGAACGTTA
GCCTCTGACCTATCAAATGCATCCCCTGGGGGATCTGAAGAGAACAAGCGTGCATACATG
AATTTAGTAAAGAAACTGAAAAAAGAGCTAGGAGACAAGCGATCAGAAAGTATTGACAAA
GTTCGACAGTTACTACCTTTGCCGAAACAGACATGTGATGTCATCACTTGTGAACCTATG
GGTTCCTTGATTGACACAAAAGGAAACAAAATTGCTGGATTTGACTCTATAGATAAAAAA
CAGGGTCTCCAGGTCTCTACGAAGCAGAAGGTGTCCCCGTGGGACTTGTTTGAGGGTCAG
AAGAACCCAGCTCCTTTGTCCTGGGCCTGGTTTGGGACAGTCCGAGTGGACCGAAGAGTG
ATCAAGTACGAGGAGCAGCATCACCTCCTGCTGTATCACACACACCCCATGCCCAAGCCC
CGCAGTTACTACCTCCAGCCACTGCCCCTGCCTCCTGAGGAGGAAGAGGAAGAGCCCACA
TCTCCAGTTTCTCAGGAACCAGAAAGGAAGTCCGCTGAGCTGTCAGATCAGGGAAAAACC
ACAACAGATGAAGAAAAGAAAACAAAAGGAAGGAAGCGCAAGACGAAATCTAGCTCAAGA
GTTGATGAATATCCACAGAGCAACATATACCGAGTGCCTCCTAATTACTCGCCTATCTCC
TCCCAAATGATGCACCATCCACAGTCCACCTTGTGGGGTTACAACCTCGTGGGCCAGCCC
CAGCAGCCCGGCTTTTTCCTTCAGAACCAATCTCTTACTCCAGGTGGCTCCAGATTGGAC
CCTGCAGGCTCCTTTGTCCCAACCAACACCAAACAAGCTCTGTCAAACATGCTACAGCGG
CGCTCAGGCGCCATGATGCAGCCGCCTTCTCTTCATGCAATCACATCGCAGCAGCAGTTG
ATACAGATGAAGCTTCTGCAGCAGCAGCAGCAACAGCGACTTCTCAGGCAAGCCCAGACT
CGGCCTTTCCAACAGGGCCAGCCGGGGGACCAGGCTGCTCTCTTTGCTGCGCAAGCACGG
CCCTCCCCTCAGCTCCCTCAGTATCCAGGGCTGCAGCAAGCACAGACCATGCCACAGGGC
TATACAATGTATGGGACACAGATGCCTTTGCAGCAGACATCGCAGCAGCAGGCTGGCAGT
GTGGTCCTGTCTCCCAGCTATAACTCCAGAGCCTATCCGGCCGCACATTCCAACCCCGTG
CTAATGGAAAGACTCAGACAGATTCAGCAGCAGCCGAGTGGCTATGTTCAGCAGCAGGCC
TCGCCGTACCTGCAGCCCCTGACTGGCTCTCAGAGACTGAACCATCAGGCTCTACAGCAG
AGCCCTCTGGTGGGCGGGGGAATTGATGCTGTGCTGACTTCTGCACATCCAAACCTTCCC
TCCGTGCCCCTGCCTCAGGATCCCATGAGACCCAGACAGCCGCAAGTTCGACAGCAGCAG
AGACTCCTCCAGATGCAGCAGCCCCAGCAGCCCCAGCCCCAGCAGCCTCCCCAGCCCCAG
CAGTCCTCGCAGTCCCAGAGTCAGACCCTTGGTCTCCAAGCAATGCAGCCCCAGCAGCCC
TTGTTTCCCAGGCAAGGCTTGCAGCAGACCCAGCAGCAGCAGCAGACGGCCGCCTTGGTG
CGGCAGCTCCAGAAGCAGCTTTCCAGCAACCAGCCACAGCAAGGAGTGACTCCGTATGGG
CATCCTTCACACTTCTTG
Protein sequence predicted based on nucleotide sequence
MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSAR NIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDL AGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQA PDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPEVEQAMKQWEYNEKLAFHM FQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCAR RLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPL VYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQV RARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSM ETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIE AERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLV LLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRPRSPVGENADEHYSKDHDVKMEIFSP MPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSS HECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQETF PTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPA LNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQ TAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQT IYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCM GHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSF HDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQ ACFLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNSVSSLK NDDFTMRGLRCDGNADDIWTASQNPKSCGKSISIETANLREYARYVLRTICQQEWVGEHC LKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQ WTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNSSNSGMSL FNPNSIGSADTSSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKG QHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNW REERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHT NNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDK VRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQ KNPAPLSWAWFGTVRVDRRVIKYEEQHHLLLYHTHPMPKPRSYYLQPLPLPPEEEEEEPT SPVSQEPERKSAELSDQGKTTTDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPIS SQMMHHPQSTLWGYNLVGQPQQPGFFLQNQSLTPGGSRLDPAGSFVPTNTKQALSNMLQR RSGAMMQPPSLHAITSQQQLIQMKLLQQQQQQRLLRQAQTRPFQQGQPGDQAALFAAQAR PSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTSQQQAGSVVLSPSYNSRAYPAAHSNPV LMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQQSPLVGGGIDAVLTSAHPNLP SVPLPQDPMRPRQPQVRQQQRLLQMQQPQQPQPQQPPQPQQSSQSQSQTLGLQAMQPQQP LFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHFL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
