Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878263
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5778nts
Open reading frame: 1 to 5778
ATGGCTCCTCGCGCCGCGGGCGGCGCACCCCTTAGCGCCCGGGCCGCCGCCGCCAGCCCC
CCGCCGTTCCAGACGCCGCCGCGGTGCCCGGTGCCGCTGCTGTTGCTGCTGCTCCTGGGG
GCGGCGCGGGCCGGCGCCCTGGAGATCCAGCGTCGGTTCCCCTCGCCCACGCCCACCAAC
AACTTCGCCCTGGACGGCGCGGCGGGGACCGTGTACCTGGCGGCCGTCAACCGCCTCTAT
CAGCTGTCGGGCGCCAACCTGAGCCTGGAGGCCGAGGCGGCCGTGGGCCCGGTGCCCGAC
AGCCCGCTGTGTCACGCTCCGCAGCTGCCGCAGGCCTCGTGCGAGCACCCGCGGCGCCTC
ACGGACAACTACAACAAGATCCTGCAGCTGGACCCCGGCCAGGGCCTGGTAGTCGTGTGC
GGGTCCATCTACCAGGGCTTCTGCCAGCTGCGGCGCCGGGGCAACATCTCGGCCGTGGCC
GTGCGCTTCCCGCCCGCCGCGCCGCCCGCCGAGCCCGTCACGGTGTTCCCCAGCATGCTG
AACGTGGCGGCCAACCACCCGAACGCGTCCACCGTGGGGCTAGTTCTGCCTCCCGCCGCG
GGCGCGGGGGGCAGCCGCCTGCTCGTGGGCGCCACGTACACCGGTTACGGCAGCTCCTTC
TTCCCGCGCAACCGCAGCCTGGAGGACCACCGCTTCGAGAACACGCCCGAGATCGCCATC
CGCTCCCTGGACACGCGCGGCGACCTGGCCAAGCTCTTCACCTTCGACCTCAACCCCTCC
GACGACAACATCCTCAAGATCAAGCAGGGCGCCAAGGAGCAGCACAAGCTGGGCTTCGTG
AGCGCCTTCCTGCACCCGTCCGACCCGCCGCCGGGTGCACAGTCCTACGCGTACCTGGCG
CTCAACAGCGAGGCGCGCGCGGGCGACAAGGAGAGCCAGGCGCGGAGCCTGCTGGCGCGC
ATCTGCCTGCCCCACGGCGCCGGCGGCGACGCCAAGAAGCTCACCGAGTCCTACATCCAG
TTGGGCTTGCAGTGCGCGGGCGGCGCGGGCCGCGGCGACCTCTACAGCCGCCTGGTGTCG
GTCTTCCCAGCCCGGGAGCGGCTCTTTGCTGTCTTCGAGCGGCCCCAGGGGTCCCCCGCG
GCCCGCGCTGCTCCGGCCGCACTCTGCGCCTTCCGCTTCGCCGACGTGCGAGCCGCCATC
CGAGCTGCGCGCACCGCCTGCTTCGTGGAACCGGCGCCCGACGTGGTGGCGGTGCTCGAC
AGCGTGGTGCAGGGCACGGGACCGGCCTGCGAGCGCAAGCTCAACATCCAGCTCCAGCCA
GAGCAGCTGGACTGTGGAGCTGCTCACCTGCAGCACCCGCTGTCCATCCTGCAGCCCCTG
AAGGCCACGCCCGTGTTCCGCGCCCCGGGCCTCACCTCCGTGGCCGTGGCCAGCGTCAAC
AACTACACAGCGGTCTTCCTGGGCACGGTCAACGGGAGGCTTCTCAAGATCAACCTGAAC
GAGAGCATGCAGGTGGTGAGCAGGCGGGTGGTGACTGTGGCCTATGGGGAGCCCGTGCAC
CATGTCATGCAGTTTGACCCAGCAGACTCCGGTTACCTTTACCTGATGACGTCCCACCAG
ATGGCCAGGGTGAAGGTCGCCGCCTGCAACGTGCACTCCACCTGTGGGGACTGCGTGGGT
GCGGCGGACGCCTACTGCGGCTGGTGTGCCCTGGAGACGCGGTGCACCTTGCAGCAGGAC
TGCACCAATTCCAGCCAGCAGCATTTCTGGACCAGTGCCAGCGAGGGCCCCAGCCGCTGT
CCTGCCATGACCGTCCTGCCTTCCGAGATCGATGTGCGCCAGGAGTACCCAGGCATGATC
CTGCAGATCTCGGGCAGCCTGCCCAGCCTCAGTGGCATGGAGATGGCCTGTGACTATGGG
AACAACATCCGCACTGTGGCTCGGGTCCCAGGCCCTGCCTTTGGTCACCAGATTGCCTAC
TGCAACCTCCTGCCGAGGGACCAGTTTCCGCCCTTCCCCCCCAACCAGGACCACGTGACT
GTTGAGATGTCTGTGAGGGTCAATGGGCGGAACATCGTCAAGGCCAATTTCACCATCTAC
GACTGCAGCCGCACTGCACAAGTGTACCCCCACACAGCCTGTACCAGCTGCCTGTCGGCA
CAGTGGCCCTGTTTCTGGTGCAGCCAGCAGCACTCCTGTGTTTCCAACCAGTCTCGGTGC
GAGGCCTCACCAAACCCCACGAGCCCTCAGGACTGCCCCCGGACCCTGCTCTCACCCCTG
GCACCCGTGCCTACGGGTGGCTCCCAGAACATCCTGGTGCCTCTGGCCAACACTGCCTTT
TTCCAGGGTGCAGCCCTGGAGTGTAGTTTTGGGCTGGAGGAGATCTTCGAGGCTGTGTGG
GTGAATGAGTCTGTTGTACGCTGTGACCAGGTGGTGCTGCACACGACCCGGAAGAGCCAG
GTGTTCCCGCTCAGCCTCCAACTAAAGGGGCGGCCAGCCCGATTCCTGGACAGCCCTGAG
CCCATGACAGTCATGGTCTATAACTGTGCCATGGGCAGCCCCGACTGTTCCCAGTGCCTG
GGCCGCGAAGACCTGGGTCACCTGTGCATGTGGAGTGATGGCTGCCGCCTGCGGGGGCCT
CTGCAGCCCATGGCTGGCACCTGCCCCGCCCCCGAGATCCGCGCGATTGAGCCCCTGAGT
GGCCCGTTGGACGGTGGGACCCTGCTGACCATCCGAGGAAGGAACCTGGGCCGGCGGCTC
AGTGACGTGGCCCACGGCGTGTGGATTGGTGGTGTGGCCTGTGAGCCACTGCCTGACAGA
TACACGGTGTCGGAGGAGATCGTGTGTGTCACAGGGCCAGCCCCAGGACCACTCTCAGGT
GTGGTGACCGTGAACGCCTCTAAGGAGGGCAAGTCCCGGGACCGCTTCTCCTACGTGCTG
CCCCTGGTCCACTCCCTGGAGCCTACCATGGGCCCCAAGGCCGGGGGCACCAGGATCACC
ATCCATGGGAATGACCTCCATGTAGGCTCCGAGCTCCAGGTCCTGGTGAACGACACAGAC
CCCTGCACGGAGCTGATGCGCACAGATACCAGCATCGCCTGCACCATGCCTGAGGGGGCC
CTGCCGGCTCCGGTGCCTGTGTGTGTGCGCTTCGAGCGTCGGGGCTGCGTGCACGGCAAC
CTCACCTTCTGGTACATGCAGAACCCGGTCATCACGGCCATCAGTCCCCGCCGCAGCCCT
GTCAGTGGCGGCAGGACCATCACAGTGGCTGGTGAGCGTTTCCACATGGTGCAGAATGTG
TCCATGGCCGTCCACCACATTGGCCGGGAGCCCACGCTCTGCAAGGTTCTCAACTCCACC
CTCATCACCTGCCCGTCCCCCGGGGCCCTGAGCAACGCATCAGCGCCAGTGGACTTCTTC
ATCAATGGGCGGGCCTACGCAGACGAGGTGGCTGTGGCTGAGGAGCTACTGGACCCCGAG
GAGGCACAGCGGGGCAGCAGGTTCCGCCTGGACTACCTCCCCAACCCACAGTTCTCTACG
GCCAAGAGGGAGAAGTGGATCAAGCACCACCCCGGGGAGCCTCTCACCCTCGTTATCCAC
AAGGAGCAGGACAGCCTGGGGCTCCAGAGTCACGAGTACCGGGTCAAGATAGGCCAAGTA
AGCTGCGACATCCAGATTGTCTCTGACAGAATCATCCACTGCTCGGTCAACGAGTCCCTG
GGCGCGGCCGTGGGGCAGCTGCCCATCACAATCCAGGTAGGGAACTTCAACCAGACCATC
GCCACACTGCAGCTGGGGGGCAGCGAGACGGCCATCATCGTGTCCATCGTCATCTGCAGC
GTCCTGCTGCTGCTCTCCGTGGTGGCCCTGTTCGTCTTCTGTACCAAGAGCCGACGTGCT
GAGCGTTACTGGCAGAAGACGCTGCTGCAGATGGAGGAGATGGAATCTCAGATCCGAGAG
GAAATCCGCAAAGGCTTCGCTGAGCTGCAGACAGACATGACAGATCTCACCAAGGAGCTG
AACCGCAGCCAGGGCATCCCCTTCCTGGAGTATAAGCACTTCGTGACCCGCACCTTCTTC
CCCAAGTGTTCCTCCCTTTATGAAGAGCGTTACGTGCTGCCCTCCCAGACCCTCAACTCC
CAGGGCAGCTCCCAGGCACAGGAAACCCACCCACTGCTGGGAGAGTGGAAGATTCCTGAG
AGCTGCCGGCCCAACATGGAAGAGGGAATTAGCTTGTTCTCCTCACTACTCAACAACAAG
CACTTCCTCATCGTCTTTGTCCACGCGCTGGAGCAGCAGAAGGACTTTGCGGTGCGCGAC
AGGTGCAGCCTGGCCTCGCTGCTGACCATCGCGCTGCACGGCAAGCTGGAGTACTACACC
AGCATCATGAAGGAGCTGCTGGTGGACCTCATTGACGCCTCGGCCGCCAAGAACCCCAAG
CTCATGCTGCGGCGCACAGAGTCTGTGGTGGAGAAGATGCTCACCAACTGGATGTCCATC
TGCATGTACAGCTGTCTGCGGGAGACGGTGGGGGAGCCATTCTTCCTGCTGCTGTGTGCC
ATCAAGCAGCAAATCAACAAGGGCTCCATCGACGCCATCACAGGCAAGGCCCGCTACACA
CTCAATGAGGAGTGGCTGCTGCGGGAGAACATCGAGGCCAAGCCCCGGAACCTGAACGTG
TCCTTCCAGGGCTGTGGCATGGACTCGCTGAGCGTGCGGGCCATGGACACCGACACGCTG
ACACAGGTCAAGGAGAAGATCCTGGAGGCCTTCTGCAAGAATGTGCCCTACTCCCAGTGG
CCGCGTGCAGAGGACGTCGACCTTGAGTGGTTCGCCTCCAGCACACAGAGCTACATCCTT
CGGGACCTGGACGACACCTCAGTGGTGGAAGACGGCCGCAAGAAGCTTAACACGCTGGCC
CATTACAAGATCCCTGAAGGTGCCTCCCTGGCCATGAGTCTCATAGACAAGAAGGACAAC
ACACTGGGCCGAGTGAAAGACTTGGACACAGAGAAGTATTTCCATTTGGTGCTGCCTACG
GACGAGCTGGCGGAGCCCAAGAAGTCTCACCGGCAGAGCCATCGCAAGAAGGTGCTCCCG
GAAATCTACCTGACCCGCCTGCTCTCCACCAAGGGCACGTTGCAGAAGTTTCTGGATGAC
CTGTTCAAGGCCATTCTGAGTATCCGTGAAGACAAGCCCCCACTGGCTGTCAAGTACTTT
TTCGACTTCCTGGAGGAGCAGGCTGAGAAGAGGGGAATCTCCGACCCCGACACCCTACAC
ATCTGGAAGACCAACAGCCTTCCTCTCCGGTTCTGGGTGAACATCCTGAAGAACCCCCAG
TTTGTCTTTGACATCGACAAGACAGACCACATCGACGCCTGCCTTTCAGTCATCGCGCAG
GCCTTCATCGACGCCTGCTCCATCTCTGACCTGCAGCTGGGCAAGGATTCGCCAACCAAC
AAGCTCCTCTACGCCAAGGAGATTCCTGAGTACCGGAAGATCGTGCAGCGCTACTACAAG
CAGATCCAGGACATGACGCCGCTCAGCGAGCAAGAGATGAATGCCCATCTGGCCGAGGAG
TCGAGGAAATACCAGAATGAGTTCAACACCAATGTGGCCATGGCAGAGATTTATAAGTAC
GCCAAGAGGTATCGGCCGCAGATCATGGCCGCGCTGGAGGCCAACCCCACGGCCCGGAGG
ACACAACTGCAGCACAAGTTTGAGCAGGTGGTGGCTTTGATGGAGGACAACATCTACGAG
TGCTACAGTGAGGCCTTG
Protein sequence predicted based on nucleotide sequence
MAPRAAGGAPLSARAAAASPPPFQTPPRCPVPLLLLLLLGAARAGALEIQRRFPSPTPTN NFALDGAAGTVYLAAVNRLYQLSGANLSLEAEAAVGPVPDSPLCHAPQLPQASCEHPRRL TDNYNKILQLDPGQGLVVVCGSIYQGFCQLRRRGNISAVAVRFPPAAPPAEPVTVFPSML NVAANHPNASTVGLVLPPAAGAGGSRLLVGATYTGYGSSFFPRNRSLEDHRFENTPEIAI RSLDTRGDLAKLFTFDLNPSDDNILKIKQGAKEQHKLGFVSAFLHPSDPPPGAQSYAYLA LNSEARAGDKESQARSLLARICLPHGAGGDAKKLTESYIQLGLQCAGGAGRGDLYSRLVS VFPARERLFAVFERPQGSPAARAAPAALCAFRFADVRAAIRAARTACFVEPAPDVVAVLD SVVQGTGPACERKLNIQLQPEQLDCGAAHLQHPLSILQPLKATPVFRAPGLTSVAVASVN NYTAVFLGTVNGRLLKINLNESMQVVSRRVVTVAYGEPVHHVMQFDPADSGYLYLMTSHQ MARVKVAACNVHSTCGDCVGAADAYCGWCALETRCTLQQDCTNSSQQHFWTSASEGPSRC PAMTVLPSEIDVRQEYPGMILQISGSLPSLSGMEMACDYGNNIRTVARVPGPAFGHQIAY CNLLPRDQFPPFPPNQDHVTVEMSVRVNGRNIVKANFTIYDCSRTAQVYPHTACTSCLSA QWPCFWCSQQHSCVSNQSRCEASPNPTSPQDCPRTLLSPLAPVPTGGSQNILVPLANTAF FQGAALECSFGLEEIFEAVWVNESVVRCDQVVLHTTRKSQVFPLSLQLKGRPARFLDSPE PMTVMVYNCAMGSPDCSQCLGREDLGHLCMWSDGCRLRGPLQPMAGTCPAPEIRAIEPLS GPLDGGTLLTIRGRNLGRRLSDVAHGVWIGGVACEPLPDRYTVSEEIVCVTGPAPGPLSG VVTVNASKEGKSRDRFSYVLPLVHSLEPTMGPKAGGTRITIHGNDLHVGSELQVLVNDTD PCTELMRTDTSIACTMPEGALPAPVPVCVRFERRGCVHGNLTFWYMQNPVITAISPRRSP VSGGRTITVAGERFHMVQNVSMAVHHIGREPTLCKVLNSTLITCPSPGALSNASAPVDFF INGRAYADEVAVAEELLDPEEAQRGSRFRLDYLPNPQFSTAKREKWIKHHPGEPLTLVIH KEQDSLGLQSHEYRVKIGQVSCDIQIVSDRIIHCSVNESLGAAVGQLPITIQVGNFNQTI ATLQLGGSETAIIVSIVICSVLLLLSVVALFVFCTKSRRAERYWQKTLLQMEEMESQIRE EIRKGFAELQTDMTDLTKELNRSQGIPFLEYKHFVTRTFFPKCSSLYEERYVLPSQTLNS QGSSQAQETHPLLGEWKIPESCRPNMEEGISLFSSLLNNKHFLIVFVHALEQQKDFAVRD RCSLASLLTIALHGKLEYYTSIMKELLVDLIDASAAKNPKLMLRRTESVVEKMLTNWMSI CMYSCLRETVGEPFFLLLCAIKQQINKGSIDAITGKARYTLNEEWLLRENIEAKPRNLNV SFQGCGMDSLSVRAMDTDTLTQVKEKILEAFCKNVPYSQWPRAEDVDLEWFASSTQSYIL RDLDDTSVVEDGRKKLNTLAHYKIPEGASLAMSLIDKKDNTLGRVKDLDTEKYFHLVLPT DELAEPKKSHRQSHRKKVLPEIYLTRLLSTKGTLQKFLDDLFKAILSIREDKPPLAVKYF FDFLEEQAEKRGISDPDTLHIWKTNSLPLRFWVNILKNPQFVFDIDKTDHIDACLSVIAQ AFIDACSISDLQLGKDSPTNKLLYAKEIPEYRKIVQRYYKQIQDMTPLSEQEMNAHLAEE SRKYQNEFNTNVAMAEIYKYAKRYRPQIMAALEANPTARRTQLQHKFEQVVALMEDNIYE CYSEAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
