Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878291
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4551nts
Open reading frame: 1 to 4551
ATGGCGCCAGCGAAAGCCACGAACGTGGTGCGGCTGCTACTAGGCTCCACAGCGCTGTGG
CTTTCGCAGCTCGGCTCCGGGACGGTCGCCGCGTCCAAGTCGGTGACTGCCCACTTGGCC
GCGAAGTGGCCCGAGACCCCGCTGCTGCTGGAGGCAAGTGAATTTATGGCAGAAGAAAGT
AATGAAAAATTTTGGCAGTTTTTGGAAACTGTGCAAGAATTAGCAATTTATAAGCAAACA
GAATCAGATTATTCTTATTACAACTTAATCCTGAAGAAAGCTGGACAGTTTCTAGACAAT
TTACACATCAACCTTTTAAAGTTTGCTTTCTCTATAAGGGCATACTCCCCAGCTATTCAG
ATGTTTCAGCAGATTGCAGCTGATGAGCCACCACCAGATGGTTGTAATGCATTTGTGGTT
ATTCATAAGAAGCACACCTGTAAAATTAATGAGATTAAAAAGCTGCTGAAGAAAGCTGCT
TCAAGGACTAGACCTTATCTATTTAAAGGAGATCACAAATTTCCTACAAACAAAGAGAAC
TTACCAGTGGTGATTCTCTATGCCGAAATGGGTACTAGAACATTTAGTGCATTTCACAAA
GTATTGTCTGAAAAAGCTCAAAATGAGGAAATTCTGTATGTTCTTCGCCATTATATTCAG
AAACCAAGCTCACGGAAAATGTACTTATCTGGGTATGGTGTGGAGCTAGCAATTAAGAGT
ACAGAATACAAAGCACTGGATGATACCCAAGTTAAAACTGTGACTAATACTACTGTAGAG
GATGAGACTGAAACAAATGAAGTTCAAGGATTTCTCTTTGGGAAACTAAAAGAAATATAT
TCAGATCTTAGAGATAATCTGACAGCATTCCAAAAATACCTGATTGAGAGTAACAAACAA
ATGATGCCTTTGAAAGTCTGGGAACTACAAGATCTTAGTTTTCAAGCAGCTTCTCAAATA
ATGTCCGCTCCAGTTTATGATTCCATTAAATTAATGAAAGACATTTCACAGAACTTCCCC
ATAAAAGCCAGATCTCTAACCAGAATTGCTGTAAATCAACATATGAGAGAAGAAATAAAG
GAAAATCAAAAGGATCTTCAAGTTAGATTTAAAATTCAGCCAGGCGATGCTCGTCTATTT
ATAAATGGCCTTCGTGTTGATATGGATGTTTATGACGCTTTTAGTATTTTGGATATGCTG
AAATTAGAAGGAAAAATGATGAATGGCCTTCGCAATCTTGGGATCAATGGGGAAGATATG
AGCAAATTTTTAAAATTAAATTCACACATTTGGGAATATACTTATGTATTAGATATTCGA
CATTCTTCTATAATGTGGATTAATGACTTAGAAAATGATGATTTGTATATTACATGGCCT
ACAAGTTGCCAGAAACTTCTGAAGCCAGTATTTCCTGGAAGTGTACCTTCCATAAGGCGC
AATTTTCATAATTTGGTTCTGTTTATTGATCCGGCCCAAGAATATACCTTGGATTTTATA
AAACTTGCTGATGTTTTCTATTCTCACGAAGTTCCTCTTAGAATTGGTTTTGTGTTCATT
CTTAATACAGATGATGAAGTTGATGGAGCAAATGATGCTGGAGTTGCTCTCTGGCGAGCT
TTCAACTATATTGCAGAAGAATTTGATATATCAGAAGCATTTATTTCTATAGTACACATG
TACCAAAAAGTGAAGAAGGATCAAAATATACTCACTGTGGACAATGTGAAGAGTGTTCTC
CAAAATACATTTCCTCATGCTAATATTTGGGATATTTTGGGAATTCATTCTAAATATGAT
GAAGAAAGAAAGGCTGGAGCAAGCTTTTATAAGATGACTGGCCTGGGTCCTTTGCCTCAA
GCTCTTTATAATGGTGAACCCTTTAAACATGAAGAGATGAATATTAAAGAACTAAAAATG
GCTGTTCTTCAAAGAATGATGGATGCATCTGTATATTTACAAAGAGAAGTTTTTTTGGGC
ACATTAAATGATCGCACGAATGCAATTGATTTTCTAATGGATAGGAATAATGTTGTACCC
CGTATAAATACTTTGATTTTGCGTACTAACCAGCAGTACCTCAATTTAATATCTACATCA
GTAACTGCTGATGTTGAAGATTTCTCTACTTTCTTTTTCTTGGATTCACAAGATAAGAGT
GCTGTAATTGCAAAGAACATGTATTATTTAACCCAAGACGATGAGAGTATAATTTCTGCA
GTCACTCTCTGGATTATTGCAGATTTTGATAAGCCTTCTGGGAGAAAACTTCTTTTTAAT
GCATTAAAGCACATGAAAACAAGTGTTCATAGTCGGTTGGGGATTATTTATAATCCTACA
TCAAAAATAAATGAAGAGAACACAGCTATTTCTAGAGGAATTTTGGCAGCTTTTCTTACA
CAGAAGAACATGTTTTTGAGAAGCTTTCTTGGGCAACTGGCAAAGGAAGAAATTGCTACA
GCTATTTACTCTGGAGATAAAATTAAAACATTCCTTATTGAGGGGATGGATAAGAATGCT
TTTGAGAAAAAATATAATACTGTTGGAGTGAATATTTTTCGAACTCACCAGTTGTTCTGT
CAAGATGTACTTAAATTACGTCCTGGAGAAATGGGTATTGTCAGCAATGGGAGATTCTTA
GGACCTTTAGATGAAGATTTTTATGCAGAAGATTTTTACTTGTTGGAAAAGATAACATTT
AGTAATTTAGGAGAGAAAATTAAAGGCATTGTTGAAAATATGGGAATCAACGCAAATAAC
ATGAGTGACTTTATTATGAAAGTTGATGCCCTTATGTCCTCTGTGCCTAAGCGTGCATCT
CGATATGATGTCACATTTCTTAGGGAGAATCACAGTGTTATAAAGACGAATCCTCAAGAG
AATGATATGTTCTTCAATGTCATTGCTATTGTTGATCCATTAACAAGAGAAGCACAGAAA
ATGGCACAGTTGTTGGTTGTACTTGGCAAGATTATCAACATGAAGATAAAGTTGTTCATG
AACTGTAGGGGCAGGCTTTCAGAAGCCCCTTTAGAAAGCTTTTACCGTTTTGTTCTGGAA
CCAGAACTGATGTCAGGGGCTAATGACGTTTCTTCTCTTGGACCAGTGGCAAAATTTTTG
GATATTCCTGAATCACCCCTCCTAATCCTCAACATGATTACTCCAGAAGGCTGGTTGGTT
GAAACAGTGCACAGCAACTGTGACCTTGATAATATTCACTTAAAGGATACTGAGAAAACT
GTTACAGCAGAATATGAACTAGAATACTTACTACTGGAAGGACAATGCTTTGATAAAGTG
ACAGAACAGCCTCCTCGGGGTCTGCAGTTCACACTAGGCACAAAAAATAAACCTGCTGTG
GTTGATACAATAGTGATGGCACATCATGGGTATTTTCAATTAAAAGCAAACCCAGGTGCT
TGGATACTGAGGTTACACCAAGGAAAATCTGAAGATATTTATCAAATAGTTGGGCATGAA
GGAACTGACTCTCAAGCAGACCTAGAAGATATCATTGTTGTATTAAACAGCTTCAAAAGC
AAGATACTCAAAGTAAAAGTGAAAAAAGAAACAGACAAAATTAAGGAAGATATCCTTACC
GATGAAGATGAAAAAACAAAAGGACTGTGGGATTCCATTAAAAGTTTCACAGTAAGCTTG
CATAAAGAAAACAAAAAGGAAAAAGATGTCCTAAACATTTTTTCAGTTGCTTCTGGTCAT
TTATATGAACGTTTTTTAAGAATTATGATGCTTTCTGTTTTGCGTAACACCAAAACACCA
GTGAAATTCTGGTTGCTAAAAAATTATCTCTCACCGACATTTAAAGAAGTAATTCCTCAC
ATGGCTAAAGAGTATGGATTCCGATATGAACTAGTTCAATATAGGTGGCCCCGTTGGCTT
CGTCAACAGACTGAAAGACAGAGGATTATTTGGGGTTACAAAATTCTTTTCCTTGATGTT
CTTTTCCCACTAGCAGTGGACAAAATCATTTTTGTTGATGCTGACCAGATTGTGAGACAT
GATCTAAAAGAACTTCGAGATTTCGATCTGGATGGAGCTCCTTATGGGTATACTCCATTT
TGTGATAGCCGCAGGGAAATGGATGGATATCGTTTCTGGAAAACAGGATACTGGGCATCA
CATCTTTTAAGACGGAAATACCATATCAGTGCTTTATATGTAGTGGATCTCAAGAAGTTC
AGGAGAATTGGAGCAGGTGACAGGCTCAGGAGCCAGTATCAAGCTCTCAGTCAAGATCCA
AACAGTCTTTCAAACCTAGATCAGGATCTCCCCAATAATATGATTTACCAAGTCGCCATT
AAGTCTCTTCCTCAAGACTGGCTGTGGTGTGAAACCTGGTGTGATGATGAATCCAAACAA
AGAGCCAAAACAATTGATCTGTGCAATAATCCCAAAACAAAAGAATCCAAACTAAAAGCT
GCTGCCAGAATTGTCCCAGAATGGGTGGAGTATGATGCTGAGATAAGACAACTATTAGAT
CATCTTGAAAACAAGAAGCAAGATACAATTTTGACACATGATGAACTCTTG
Protein sequence predicted based on nucleotide sequence
MAPAKATNVVRLLLGSTALWLSQLGSGTVAASKSVTAHLAAKWPETPLLLEASEFMAEES NEKFWQFLETVQELAIYKQTESDYSYYNLILKKAGQFLDNLHINLLKFAFSIRAYSPAIQ MFQQIAADEPPPDGCNAFVVIHKKHTCKINEIKKLLKKAASRTRPYLFKGDHKFPTNKEN LPVVILYAEMGTRTFSAFHKVLSEKAQNEEILYVLRHYIQKPSSRKMYLSGYGVELAIKS TEYKALDDTQVKTVTNTTVEDETETNEVQGFLFGKLKEIYSDLRDNLTAFQKYLIESNKQ MMPLKVWELQDLSFQAASQIMSAPVYDSIKLMKDISQNFPIKARSLTRIAVNQHMREEIK ENQKDLQVRFKIQPGDARLFINGLRVDMDVYDAFSILDMLKLEGKMMNGLRNLGINGEDM SKFLKLNSHIWEYTYVLDIRHSSIMWINDLENDDLYITWPTSCQKLLKPVFPGSVPSIRR NFHNLVLFIDPAQEYTLDFIKLADVFYSHEVPLRIGFVFILNTDDEVDGANDAGVALWRA FNYIAEEFDISEAFISIVHMYQKVKKDQNILTVDNVKSVLQNTFPHANIWDILGIHSKYD EERKAGASFYKMTGLGPLPQALYNGEPFKHEEMNIKELKMAVLQRMMDASVYLQREVFLG TLNDRTNAIDFLMDRNNVVPRINTLILRTNQQYLNLISTSVTADVEDFSTFFFLDSQDKS AVIAKNMYYLTQDDESIISAVTLWIIADFDKPSGRKLLFNALKHMKTSVHSRLGIIYNPT SKINEENTAISRGILAAFLTQKNMFLRSFLGQLAKEEIATAIYSGDKIKTFLIEGMDKNA FEKKYNTVGVNIFRTHQLFCQDVLKLRPGEMGIVSNGRFLGPLDEDFYAEDFYLLEKITF SNLGEKIKGIVENMGINANNMSDFIMKVDALMSSVPKRASRYDVTFLRENHSVIKTNPQE NDMFFNVIAIVDPLTREAQKMAQLLVVLGKIINMKIKLFMNCRGRLSEAPLESFYRFVLE PELMSGANDVSSLGPVAKFLDIPESPLLILNMITPEGWLVETVHSNCDLDNIHLKDTEKT VTAEYELEYLLLEGQCFDKVTEQPPRGLQFTLGTKNKPAVVDTIVMAHHGYFQLKANPGA WILRLHQGKSEDIYQIVGHEGTDSQADLEDIIVVLNSFKSKILKVKVKKETDKIKEDILT DEDEKTKGLWDSIKSFTVSLHKENKKEKDVLNIFSVASGHLYERFLRIMMLSVLRNTKTP VKFWLLKNYLSPTFKEVIPHMAKEYGFRYELVQYRWPRWLRQQTERQRIIWGYKILFLDV LFPLAVDKIIFVDADQIVRHDLKELRDFDLDGAPYGYTPFCDSRREMDGYRFWKTGYWAS HLLRRKYHISALYVVDLKKFRRIGAGDRLRSQYQALSQDPNSLSNLDQDLPNNMIYQVAI KSLPQDWLWCETWCDDESKQRAKTIDLCNNPKTKESKLKAAARIVPEWVEYDAEIRQLLD HLENKKQDTILTHDELL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
