Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878313
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7521nts
Open reading frame: 1 to 7521
ATGGAGGTGCTTCAGTGTGATGGCTGTGATTTCCGAGCCCCGTCTTATGAAGATCTCAAG
GCACACATTCAGGATGTCCACACGGCATTTCTGCAGCCAACTGATGTTGCTGAGGACAAT
GTGAATGAGCTACGATGTGGGTCCGTGAATGCCAGTAATCAGACAGAGGTGGAGTTTTCT
TCTATAAAGGATGAATTTGCCATTGCAGAAGATTTATCAGGTCAAAATGCAACTTCATTG
GGGACCGGAGGTTACTATGGCCACAGTCCAGGATATTATGGTCAGCATATTGCCGCTAAT
CCCAAACCAACAAACAAGTTTTTTCAATGCAAGTTCTGTGTACGCTACTTCAGGTCAAAA
AACCTCCTCATAGAACACACTAGAAAGGTCCATGGAGCTCAAGCTGAAGGGAGTTCATCA
GGACCCCCTGTCCCGGGATCCTTAAATTATAATATCATGATGCACGAGGGATTTGGAAAG
GTCTTCTCTTGCCAGTTTTGCACATACAAGTCACCAAGAAGGGCAAGAATAATTAAGCAT
CAGAAGATGTATCACAAAAACAATTTGAAGGAGACCACTGCTCCCCCACCTGCTCCTGCT
CCAATGCCAGACCCTGTGGTTCCGCCCGTATCACTGCAGGACCCCTGCAAGGAACTGCCA
GCAGAGGTTGTGGAGCGCAGCATCTTAGAGTCTATGGTCAAGCCTTTGACCAAATCTCGA
GGCAACTTTTGTTGTGAGTGGTGCAGCTACCAGACCCCCCGCCGAGAACGCTGGTGTGAC
CACATGATGAAGAAACACCGCAGTATGGTCAAGATCCTTTCCAGTCTCAGACAGCAACAA
GAAGGAACTAATCTACCTGATGTGCCGAACAAGAGTGCCCCCAGCCCCACTTCCAACTCC
ACCTATCTGACCATGAATGCTGCAAGCCGGGAGATACCCAATACTACCGTCTCCAACTTC
AGGGGCTCCATGGGCAACTCCATCATGAGACCCAATTCTTCAGCTTCCAAGTTTTCGCCC
ATGTCTTACCCTCAGATGAAGCCGAAGTCACCTCACAATTCTGGTCTAGTTAACTTGACA
GAGAGATCCCGTTATGGAATGACTGACATGACCAATTCTTCTGCTGACCTGGAAACTAAC
AGCATGCTAAATGACTCTAGTTCTGATGAAGAGTTAAATGAAATAGACAGTGAGAATGGT
TTAAGTGCTATGGATCACCAGACATCAGGCCTGTCTGCAGAGCAGCTGATGGGCTCAGAT
GGCAACAAATTATTGGAGACCAAGGGGATTCCATTTAGAAGATTCATGAATAGGTTCCAG
TGCCCCTTTTGTCCTTTCCTCACCATGCATCGACGTAGCATCTCTCGTCACATAGAAAAC
ATCCACTTATCTGGAAAGACAGCTGTCTACAAATGTGACGAATGTCCGTTTACTTGCAAG
AGCTCGTTGAAACTTGGGGCTCACAAACAGTGTCACACGGGTACAACGTCAGATTGGGAT
GCTGTGAATTCCCAGAGTGAAAGCATTTCTTCCTCACTGAATGAAGGTGTGGTGTCTTAT
GAGAGCTCAAGCATCAATGGTAGAAAGTCAGGAGTCATGTTGGATCCCTTGCAGCAGCAA
CAGCCACCGCAGCCACCACCACCGCCGCCGCCACCACCACCATCACAGCCACAGCCACTG
CAGCAGCCACAGCCACCACAGCTGCAGCCACCACATCAGGTGCCACCCCAGCCACAAACA
CAGCCACCACCAACGCAGCAGCCACAGCCACCCACACAAGCCGCACCTCTGCACCCATAC
AAATGCACCATGTGTAATTACTCCACCACAACTCTGAAAGGGCTAAGAGTCCATCAGCAG
CATAAACATTCATTCTGTGACAACTTGCCAAAATTCGAGGGGCAGCCCTCAAGCCTACCA
TTGGAAAATGAGACAGACAGCCACCCCTCTTCCAGCAACACTGTGAAGAAAAGTCAGACC
TCAATTCTTGGGTTGTCCTCCAAGAACAATTTTGTAGCTAAAGCCTCTAGGAAGCTCGCC
AATGACTTTCCTCTAGATTTGTCACCCGTGAAGAAGAGAACCAGGATTGACGAGATAGCA
AGCAACCTTCAGAGCAAAATTAACCAAACCAAACAGCAGGAAGATGCAGTGATCAATGTT
GAGGATGATGAAGAGGAAGAGGAAGACAACGAAGTCGAGATAGAGGTTGAGTTGGACAGG
GAGGAAGAACCGACAGAACCCATCATAGAGGTTCCCACTTCCTTTTCTGCCCAACAGATA
TGGGTAAGAGATACCAGTGAGCCCCAGAAAGAGCCCAACTTCAGAAACATCACCCACGAT
TACAATGCCACCAATGGGGCTGAGATTGAGCTCACCCTTTCTGAAGATGAAGAGGATTAT
TATGGCTCCTCAACAAACTTGAAAGATCACCAAGTTTCCAATACTGCTCTGCTGAATACC
CAAACTCCCATCTATGGGACTGAGCACAATAGTGAAAACACAGACTTTGGTGACTCTGGA
AGGCTTTACTATTGTAAACACTGTGACTTTAACAACAAATCTGCCCGGAGTGTTAGCACC
CACTACCAACGAATGCACCCATACATTAAATTCAGCTTTAGGTACATCTTGGACCCCAAT
GATCACAGTGCAGTGTACAGGTGCCTGGAATGCTACATCGATTACACCAACTTCGAAGAT
CTCCAGCAGCATTATGGCGAGCACCACCCAGAAGCCATGAATGTACTCAACTTTGATCAC
TCGGACCTGATCTACCGGTGTCGGTTTTGTTCATACACGAGCCCGAATGTTAGAAGCCTG
ATGCCACATTACCAAAGAATGCATCCCACGGTGAAGATCAACAACGCGATGATATTTTCA
AGCTATGTCGTGGAGCAGCAGGAAGGGCTGAATACAGAATCCCAGACCCTGAGGGAGATT
CTGAATTCGGCTCCCAAGAACATGGCGACTTCCACACCTGTGGCTCGTGGTGGTGGTTTG
CCAGCTACGTTCAACAAAAACACTCCTAAGACCTTTACTCCTGAATGTGAAAATCAGAAG
GACCCTTTGGTCAACACTGTTGTTGTTTATGATTGTGATGTTTGTTCGTTTGCAAGCCCC
AACATGCATTCTGTCTTGGTTCATTATCAGAAGAAACACCCCGAAGAAAAGGCTTCCTAC
TTTAGGATCCAGAAAACTATGCGAATGGTGTCTGTGGACAGGGGCTCTGCCCTTTCTCAA
TTATCATTTGAGGTGGGTGCTCCAATGTCTCCCAAAATGTCCAACATGGGTTCCCCACCC
CCCCCACAACCCCCGCCACCAGACCTCAGTACTGAGCTTTACTACTGCAAACACTGTTCC
TACAGCAATCGGTCAGTTGTGGGAGTGCTTGTCCACTACCAGAAAAGACACCCAGAAATA
AAGGTTACTGCCAAATATATCAGACAGGCTCCTCCCACAGCTGCAATGATGAGAGGGGTC
GAAGGGCCCCAAGGCTCCCCCCGGCCACCCGCCCCCATACAACAGCTGAACCGAAGCAGC
TCTGAGAGAGATGGCCCTCCTGTGGAGAATGAGATGTTCTTTTGCCAGCACTGTGATTAT
GGGAACCGGACGGTCAAAGGGGTACTCATTCATTATCAGAAGAAGCACCGAGACTTCAAG
GCCAATGCAGATGTGATCCGGCAGCATACGGCCACCATTCGAAGCCTCTGCGACCGAAAT
CAGAAGAAGCCTGCCAGCTGCGTGCTTGTCTCCCCCTCTAATCTGGAGCGGGACAAAACG
AAACTCCGAGCACTCAAATGTAGGCAGTGCTCATATACCTCCCCCTACTTCTATGCACTG
AGGAAGCATATCAAGAAAGACCACCCCGCCCTGAAAGCCACAGTCACGTCCATCATGCGA
TGGGCATTTCTAGATGGCTTGATAGAAGCTGGCTACCACTGCGAGTGGTGCATCTACTCC
CATACGGAGCCCAACGGTTTGCTCCTGCATTACCAACGGAGGCATCCAGAACACTATGTT
GATTACACCTACATGGCTACTAAACTGTGGGCTGGGCCAGACCCATCCCCTCCCTCTCTC
ACAATGCCAGCCGAAGCCAAAACCTACAGATGCAGGGACTGTGTTTTCGAAGCTGTTTCC
ATCTGGGACATCACTAATCACTACCAAGCATTCCACCCCTGGGCCATGAATGGTGATGAG
TCAGTGCTACTGGACATCATCAAGGAGAAAGATGCTGTGGAGAAGCCCATTCTTTCATCC
GAAGAGTTGGCAGGCCCTGTGAATTGTGAAAACAGTATACCCACCCCTTTCCCGGAGCAG
GAAGCTGAATGTCCAGAGGATGCAAGACTGTCCCCTGAGAAAAGCCTGCAGCTAGCTTCA
GCCAACCCCGCCATATCCTCCACCCCATACCAGTGCACGGTATGCCAATCTGAGTATAAC
AACTTGCACGGCCTTCTCACTCATTATGGGAAGAAGCACCCTGGCATGAAAGTGAAGGCT
GCTGACTTTGCCCAGGACATTGACATCAACCCAGGTGCCGTCTACAAATGCAGGCATTGC
CCATACATCAACACCCGCATCCACGGCGTACTGACCCACTACCAGAAGCGACACCCGTCC
ATCAAGGTGACCGCTGAGGACTTTGTGCACGACGTAGAGCAGTCTGCTGACATATCCCAG
AATGACGTGGAGGAGACGAGCAGGATCTTCAAGCAAGGGTATGGCGCCTACCGGTGCAAA
CTGTGTCCGTACACACACGGCACTTTGGAGAAACTAAAAATCCACTACGAGAAGTATCAC
AATCAGCCTGAATTTGATGTCTTTTCCCAGTCGCCCCCGAAGCTGCCAGTCCCCCTCGAG
CCCGAGATGACCACTGAAGTGAGCCCTTCCCAAGTCTCCATCACTGAGGAGGAGGTGGGA
GAGGAGCCCGTGTCCACTTCTCACTTCTCTACCTCCCACCTGGTCTCCCACACTGTGTTC
CGGTGCCAGCTCTGCAAGTACTTCTGCTCCACGAGGAAGGGGATCGCCAGGCACTACCGC
ATCAAGCACAATAATGTCCGAGCCCAGCCAGAAGGCAAGAACAACCTCTTCAAGTGTGCC
CTGTGTGCCTACACCAACCCCATCCGCAAAGGTCTGGCAGCCCACTACCAGAAGCGCCAC
GACATTGATGCGTATTACACTCACTGCTTGGCAGCCTCCAGGACCATCAGCGACAAGCCC
AACAAAGTGATCATCCCATCCCCGCCCAAGGACGACTCCCCTCAGCTGAGCGAGGAACTC
CGGCGGGCAGTGGAGAAGAAAAAGTGCTCCTTGTGCTCTTTCCAGTCGTTCAGCAAGAAG
GGCATCGTGTCCCATTACATGAAACGCCACCCAGGGGTGTTCCCAAAGAAGCAGCACGCC
AGCAAGTTGGGGGGCTACTTCACGGCCGTCTATGCAGATGAGCATGAGAAGCCCACACTG
ATGGAAGAAGAGGAGAGAGGCAACTTTGAGAAAGCCGAGGTGGAGGGTGAAGCTCAGGAA
ATCGAGTGGCTCCCATTCCGCTGCATCAAATGCTTCAAGCTGTCCTTTAGCACTGCAGAG
CTGCTGTGCATGCATTACACTGACCACCACAGTCGGGACCTAAAGAGGGACTTCATCATT
CTGGGCAACGGCCCCCGCTTGCAGAACTCCACCTACCAGTGTAAGCACTGTGATAGCAAA
CTGCAAAGCACAGCCGAGCTGACCTCACACTTGAACATTCACAATGAGGAATTCCAGAAG
CGTGCCAAACGTCAGGAGAGGAGGAAACAGCTTTTGAGCAAGCAGAAATATGCAGATGGT
GCTTTTGCAGATTTCAAACAAGAGAGGCCTTTTGGTCACTTAGAAGAGGTGCCAAAGATC
AAGGAGAGGAAAGTGGTGGGCTACAAATGTAAATTCTGTGTGGAAGTGCACCCAACGCTC
CGAGCCATCTGCAATCACCTCCGAAAGCACGTCCAGTATGGCAATGTCCCAGCTGTGTCA
GCTGCTGTGAAGGGGCTGCGTTCTCATGAGAGGAGCCACCTGGCCCTGGCCATGTTTACC
CGCGAGGACAAGTACAGCTGCCAGTATTGCTCGTTTGTTTCTGCTTTCAGGCACAATTTG
GATCGCCATATGCAAACCCACCACGGACACCATAAACCATTCCGATGCAAACTCTGCTCC
TTCAAGTCCTCCTATAACAGCCGGCTGAAAACACATATACTCAAAGCTCATGCTGGTGAG
CATGCCTACAAGTGTTCTTGGTGCTCATTCTCCACCATGACAATCAGCCAGCTGAAGGAA
CACTCCCTCAAGGTCCACGGAAAAGCCCTGACCCTCCCCAGGCCACGGATCGTCAGTCTC
CTCTCCTCACACTCCCACCACTCCTCCCAAAAAGCTACCCCGGCTGAAGAAGTGGAAGAC
TCCAATGACTCATCATATTCAGAGCCCCCAGATGTTCAGCAGCAGTTGAACCACTATCAG
TCAGCTGCCCTGGCAAGGAACAACAGCCGTGTTAGCCCTGTGCCTCTTTCTGGGGCTGCT
GCTGGCACTGAGCAGAAAACTGAAGCCGTGCTTCACTGCGAATTCTGTGAATTCTCCTCC
GGCTACATCCAGAGCATCAGGCGTCATTACCGGGACAAGCATGGTGGGAAGAAGCTTTTC
AAGTGCAAAGACTGCTCCTTTTACACAGGCTTTAAATCTGCTTTTACTATGCACGTGGAA
GCTGGGCACTCAGCAGTTCCCGAGGAGGGCCCCAAAGATCTTCGCTGTCCTCTCTGCCTC
TATCACACCAAATACAAGCGCAACATGATTGACCACATCGTGCTGCACCGAGAAGAGCGT
GTTGTCCCCATTGAAGTTTGCCGGTCCAAACTGTCCAAATACTTGCAGGGAGTAGTTTTC
CGCTGTGATAAGTGTACCTTCACCTGCTCCAGTGATGAGAGCCTCCAGCAACATATAGAA
AAGCACAATGAACTGAAACCTTACAAATGCCAGCTCTGCTACTATGAGACCAAGCACACG
GAGGAACTGGACAGCCACCTTCGGGATGAGCATAAGGTAAGCCGTAACTTTGAGCTGGTT
GGACGGGTTAACTTGGATCAGCTGGAACAGATGAAGGAGAAAATGGAGAGCTCCAGCAGC
GATGATGAGGACAAGGAAGAAGAAATGAACAGCAAGGCTGAAGACAGAGAGCTGATGAGA
TTTTCTGACCACGGGGCTGCTCTTAACACTGAGAAGCGTTTTCCATGTGAATTTTGTGGA
CGGGCGTTTTCACAGGGCTCTGAGTGGGAAAGACATGTGCTGAGACACGGCATGGCATTG
AATGACACCAAGCAGGTGAGCAGAGAAGAAATCCACCCAAAAGAGATCATGGAGAACAGT
GTTAAAATGCCCTCCATAGAGGAAAAGGAAGATGACGAGGCCATTGGGATAGACTTTTCC
CTAAAGAATGAAACAGTAGCCATCTGTGTAGTAACTGCCGACAAATCTCTCCTGGAGAAT
GCAGAGGCCAAAAAAGAATTG
Protein sequence predicted based on nucleotide sequence
MEVLQCDGCDFRAPSYEDLKAHIQDVHTAFLQPTDVAEDNVNELRCGSVNASNQTEVEFS SIKDEFAIAEDLSGQNATSLGTGGYYGHSPGYYGQHIAANPKPTNKFFQCKFCVRYFRSK NLLIEHTRKVHGAQAEGSSSGPPVPGSLNYNIMMHEGFGKVFSCQFCTYKSPRRARIIKH QKMYHKNNLKETTAPPPAPAPMPDPVVPPVSLQDPCKELPAEVVERSILESMVKPLTKSR GNFCCEWCSYQTPRRERWCDHMMKKHRSMVKILSSLRQQQEGTNLPDVPNKSAPSPTSNS TYLTMNAASREIPNTTVSNFRGSMGNSIMRPNSSASKFSPMSYPQMKPKSPHNSGLVNLT ERSRYGMTDMTNSSADLETNSMLNDSSSDEELNEIDSENGLSAMDHQTSGLSAEQLMGSD GNKLLETKGIPFRRFMNRFQCPFCPFLTMHRRSISRHIENIHLSGKTAVYKCDECPFTCK SSLKLGAHKQCHTGTTSDWDAVNSQSESISSSLNEGVVSYESSSINGRKSGVMLDPLQQQ QPPQPPPPPPPPPPSQPQPLQQPQPPQLQPPHQVPPQPQTQPPPTQQPQPPTQAAPLHPY KCTMCNYSTTTLKGLRVHQQHKHSFCDNLPKFEGQPSSLPLENETDSHPSSSNTVKKSQT SILGLSSKNNFVAKASRKLANDFPLDLSPVKKRTRIDEIASNLQSKINQTKQQEDAVINV EDDEEEEEDNEVEIEVELDREEEPTEPIIEVPTSFSAQQIWVRDTSEPQKEPNFRNITHD YNATNGAEIELTLSEDEEDYYGSSTNLKDHQVSNTALLNTQTPIYGTEHNSENTDFGDSG RLYYCKHCDFNNKSARSVSTHYQRMHPYIKFSFRYILDPNDHSAVYRCLECYIDYTNFED LQQHYGEHHPEAMNVLNFDHSDLIYRCRFCSYTSPNVRSLMPHYQRMHPTVKINNAMIFS SYVVEQQEGLNTESQTLREILNSAPKNMATSTPVARGGGLPATFNKNTPKTFTPECENQK DPLVNTVVVYDCDVCSFASPNMHSVLVHYQKKHPEEKASYFRIQKTMRMVSVDRGSALSQ LSFEVGAPMSPKMSNMGSPPPPQPPPPDLSTELYYCKHCSYSNRSVVGVLVHYQKRHPEI KVTAKYIRQAPPTAAMMRGVEGPQGSPRPPAPIQQLNRSSSERDGPPVENEMFFCQHCDY GNRTVKGVLIHYQKKHRDFKANADVIRQHTATIRSLCDRNQKKPASCVLVSPSNLERDKT KLRALKCRQCSYTSPYFYALRKHIKKDHPALKATVTSIMRWAFLDGLIEAGYHCEWCIYS HTEPNGLLLHYQRRHPEHYVDYTYMATKLWAGPDPSPPSLTMPAEAKTYRCRDCVFEAVS IWDITNHYQAFHPWAMNGDESVLLDIIKEKDAVEKPILSSEELAGPVNCENSIPTPFPEQ EAECPEDARLSPEKSLQLASANPAISSTPYQCTVCQSEYNNLHGLLTHYGKKHPGMKVKA ADFAQDIDINPGAVYKCRHCPYINTRIHGVLTHYQKRHPSIKVTAEDFVHDVEQSADISQ NDVEETSRIFKQGYGAYRCKLCPYTHGTLEKLKIHYEKYHNQPEFDVFSQSPPKLPVPLE PEMTTEVSPSQVSITEEEVGEEPVSTSHFSTSHLVSHTVFRCQLCKYFCSTRKGIARHYR IKHNNVRAQPEGKNNLFKCALCAYTNPIRKGLAAHYQKRHDIDAYYTHCLAASRTISDKP NKVIIPSPPKDDSPQLSEELRRAVEKKKCSLCSFQSFSKKGIVSHYMKRHPGVFPKKQHA SKLGGYFTAVYADEHEKPTLMEEEERGNFEKAEVEGEAQEIEWLPFRCIKCFKLSFSTAE LLCMHYTDHHSRDLKRDFIILGNGPRLQNSTYQCKHCDSKLQSTAELTSHLNIHNEEFQK RAKRQERRKQLLSKQKYADGAFADFKQERPFGHLEEVPKIKERKVVGYKCKFCVEVHPTL RAICNHLRKHVQYGNVPAVSAAVKGLRSHERSHLALAMFTREDKYSCQYCSFVSAFRHNL DRHMQTHHGHHKPFRCKLCSFKSSYNSRLKTHILKAHAGEHAYKCSWCSFSTMTISQLKE HSLKVHGKALTLPRPRIVSLLSSHSHHSSQKATPAEEVEDSNDSSYSEPPDVQQQLNHYQ SAALARNNSRVSPVPLSGAAAGTEQKTEAVLHCEFCEFSSGYIQSIRRHYRDKHGGKKLF KCKDCSFYTGFKSAFTMHVEAGHSAVPEEGPKDLRCPLCLYHTKYKRNMIDHIVLHREER VVPIEVCRSKLSKYLQGVVFRCDKCTFTCSSDESLQQHIEKHNELKPYKCQLCYYETKHT EELDSHLRDEHKVSRNFELVGRVNLDQLEQMKEKMESSSSDDEDKEEEMNSKAEDRELMR FSDHGAALNTEKRFPCEFCGRAFSQGSEWERHVLRHGMALNDTKQVSREEIHPKEIMENS VKMPSIEEKEDDEAIGIDFSLKNETVAICVVTADKSLLENAEAKKEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
