Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878343
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6351nts
Open reading frame: 1 to 6351
ATGTCGGGGTTCAGGCTGGGTGACCACGTGTGGCTGGAGCCTCCCTCCACCCACAAGACC
GGCGTGGCCATCGGGGGCATCATCAAAGAGGCAAAGCCAGGCAAAGTCTTGGTTGAAGAT
GACGAGGGCAAGGAACACTGGATCCGAGCAGAGGACTTTGGTGTCCTCAGTCCCATGCAC
CCCAACTCAGTCCAGGGTGTGGACGACATGATCCGCCTGGGGGACCTGAACGAGGCAGGC
ATGGTGCACAACCTCCTGATCCGCTACCAGCAGCACAAGATCTATACATACACAGGCTCC
ATCCTGGTGGCCGTCAACCCGTTCCAGGTGCTGCCGCTCTACACCCTGGAGCAGGTACAG
CTCTACTACAGCCGCCATATGGGCGAGCTGCCCCCGCATGTCTTTGCCATCGCCAACAAC
TGCTACTTCAGCATGAAGAGGAACAAGAGGGACCAGTGCTGCATCATCAGCGGCGAGTCT
GGGGCTGGCAAGACGGAGACCACCAAGCTCATCCTGCAGTTCCTGGCCACCATCAGTGGC
CAGCATTCGTGGATTGAGCAGCAGGTCCTGGAAGCCAACCCCATCCTGGAGGCCTTTGGA
AATGCCAAGACAATCCGCAACGACAACTCAAGCCGCTTTGGGAAGTACATTGACATCTAC
TTTAACCCCAGCGGGGTGATCGAGGGCGCGCGCATCGAGCAATTTCTCCTGGAGAAGTCC
CGGGTCTGCCGGCAGGCTCCCGAGGAGCGGAACTACCATATCTTCTACTGCATGCTCATG
GGGGTGAGTGCTGAGGACAAGCAGCTGCTGAGCCTGGGCACGCCCTCCGAGTACCACTAC
CTGACCATGGGGAACTGCACTTCCTGTGAGGGGCTCAACGACGCCAAGGACTACGCCCAC
ATCCGCTCGGCCATGAAGATCCTCCAGTTCTCCGACTCCGAGAGCTGGGACGTCATCAAG
CTGCTGGCTGCCATTCTCCACCTGGGGAATGTGGGGTTCATGGCTTCGGTCTTCGAGAAC
CTGGACGCCTCAGACGTGATGGAGACGCCCGCCTTTCCCACCGTGATGAAGTTACTGGAG
GTGCAGCACCAGGAGCTCCGGGACTGTCTGATCAAGCACACCATCCTCATCCGAGGGGAA
TTTGTCACCAGGTCCCTGAACATTGCCCAGGCTGCTGACCGGAGGGACGCCTTTGTCAAG
GGCATCTATGGGCACCTCTTCCTGTGGATTGTCAAGAAGATCAATGCCGCCATCTTCACA
CCACCAGCCCAGGACCCCAAAAATGTGCGGAGGGCCATCGGCCTCCTGGACATATTTGGC
TTTGAAAATTTCGAGAACAATAGCTTCGAGCAGCTCTGCATCAACTTCGCCAACGAGCAC
CTGCAGCAGTTCTTTGTGCAGCACGTGTTCACCATGGAGCAAGAGGAGTACCGCTCGGAG
AACATCTCCTGGGACTATATCCACTACACCGACAATCGGCCCACCCTGGACCTGCTGGCC
CTCAAGCCCATGAGCATCATCTCCCTCCTGGACGAAGAAAGCCGCTTCCCGCAGGGGACA
GATCTCACCATGCTGCAAAAGCTGAACAGCGTCCATGCCAACAACAAGGCCTTCCTACAG
CCCAAGAACATCCACGATGCCAGATTTGGCATTGCCCATTTTGCCGGCGAGGTGTACTAC
CAAGCAGAAGGCTTCCTGGAGAAGAACCGAGACGTGCTGAGCACAGATATCCTCACCCTG
GTTTACTCCTCCAAAAACAAGTTTCTGAGGGAGATATTCAACTTGGAGTTAGCAGAGACC
AAGCTGGGCCATGGGACCATCCGCCAGGCAAAGGCAGGAAACCATCTCTTCAAGTCTGCA
GACTCAAATAAACGGCCCTCCACCTTAGGAAGCCAGTTCAAACAGTCTCTGGACCAGCTG
ATGAAAATCCTGACCAACTGCCAGCCTTACTTCATCCGCTGCATCAAACCTAATGAGTAC
AAGAAGCCGCTGCTGTTCGACCGGGAGCTGTGCCTGCGGCAGCTGCGATACTCGGGCATG
ATGGAGACCGTGCACATCCGCAAGTCGGGCTTCCCCATCCGCTACACGTTCGAGGAGTTC
TCGCAGAGGTTCGGCGTGTTGCTGCCCAACGCCATGCGGATGCAGCTGCAAGGCAAGCTC
CGCCAGATGACCCTGGGCATCACTGACGTGTGGCTGCGGACAGACAAAGACTGGAAAGCG
GGGAAGACAAAAATTTTCCTGAGGGATCATCAGGACACTCTGCTGGAGGTACAGAGAAGC
CAGGTGCTAGACAGAGCGGCGCTCAGCATCCAGAAAGTCCTTCGGGGCTACAGATACAGG
AAGGAGTTCCTGAGGCAGAGGCGGGCAGCTGTGACCCTGCAGGCCTGGTGGAGAGGCTAC
TGCAACAGGAGGAATTTCAAGCTGATCCTCGTGGGCTTTGAGCGCCTGCAGGCTATTGCC
CGGAGCCAGCCGCTGGCGAGGCAGTACCAGGCCATGCGGCAGAGGACAGTCCAGCTGCAG
GCCCTGTGCAGGGGATACCTGGTGCGCCAGCAAGTCCAGGCCAAGAGGAGGGCAGTGGTG
GTCATTCAGGCCCATGCCAGGGGCATGGCTGCCCGGCGCAACTTCCAGCAAAGGAAGGCC
AATGCGCCGCTGGTCATCCCGGCCGAGGGGCAGAAAAGCCAAGGCGCTCTCCCTGCCAAG
AAGCGCAGATCCATCTACGACACCGTCACTGACACGGAGATGGTGGAGAAGGTGTTCGGC
TTCCTCCCTGCCATGATTGGGGGCCAGGAGGGCCAGGCCTCGCCGCACTTTGAGGATCTG
GAATCGAAGACCCAGAAGCTGCTTGAGGTTGACCTGGACACAGTCCCCATGGCGGAGGAG
CCTGAGGAGGATGTGGATGGCCTGGCCGAGTACACCTTCCCCAAGTTTGCTGTGACTTAC
TTCCAGAAATCAGCCAGCCACACACACATCCGGCGGCCCCTCCGATACCCGTTGCTTTAC
CACGAAGATGACACTGACTGCTTGGCCGCCCTGGTCATATGGAACGTCATCCTGAGGTTC
ATGGGTGATCTCCCAGAGCCAGTGCTGTATGCCAGGAGCAGCCAGCAGGGCAGCTCAGTG
ATGCGGCAGATCCATGACACGCTGGGCAGGGAGCACGGTGCCCAGGTTCCACAGCACAGT
AGATCTGCACAGGTGGCCAGCCAGCTGAACATTGGAGAGGAGGCATTGGAGCCTGATGGC
CTTGGTGCAGACCGGCCCATGTCCAACCTGGAGAAGGTGCACTTCATCGTGGGCTACGCC
ATCCTGCGGCCCAGCCTCAGGGATGAGATTTACTGCCAGATCTGCAAGCAGCTCTCGGAG
AACTTCAAAACAAGCAGCCTGGCCCGGGGCTGGATCCTGCTCAGCCTCTGCCTCGGCTGC
TTCCCACCCTCAGAGAGGTTCATGAAGTATCTACTGAACTTCATCGGCCAAGGGCCGGCG
ACCTACGGCCCCTTCTGTGCCGAGCGCCTGAGACGCACCTATGCCAATGGGGTGCGTGCG
GAGCCCCCCACCTGGCTGGAGCTGCAGGCTGTCAAGTCCAAGAAGCACATCCCCATCCAA
GTCATCTTGGCCACTGGAGAGAGCCTAACCGTCCCCGTGGACTCAGCCTCCACATCTCGG
GAAATGTGCATGCACATCGCTCACAAGCAGGGCCTCAGCGACCACCTGGGCTTCTCCCTC
CAGGTCGCCGTGTACGACAAGTTCTGGTCCCTGGGCAGCGGGCGCGACCACATGATGGAT
GCCATCGCCCGGTGTGAGCAGATGGCCCAGGAGAGGGGCGAGAGCCAGCGCCAGTCACCC
TGGCGCATCTACTTCCGGAAGGAATTCTTCACCCCCTGGCACGACTCCCGGGAGGACCCT
GTCAGCACCGAGCTTATTTACCGCCAAGTCCTCCGAGGAGTCTGGTCTGGCGAGTACAGC
TTCGAGAAGGAGGAAGAGCTGGTTGAGCTGCTGGCCCGGCACTGCTACGTGCAGCTCGGC
GCCTCAGCAGAGAGCAAGGCTGTCCAGGAGCTGCTGCCCAGCTGCATCCCCCACAAGCTG
TACAGGACCAAGCCCCCAGACAGGTGGGCGAGCCTCGTCACTGCCGCCTGCGCCAAGGCC
CCATACACTCAGAAGCAAGTCACACCACTGGCCGTGCGAGAGCAGGTGGTGGACGCCGCC
CGCCTGCAGTGGCCGCTGCTCTTCTCCCGGCTCTTCGAAGTCATCACACTCTCAGGCCCC
CGCCTGCCCAAGACGCAGCTGATCTTGGCTGTTAACTGGAAGGGGCTTTGCTTCCTGGAC
CAGCAGGAGAAGATGCTGCTGGAACTCTCTTTCCCAGAGGTCATGGGTCTGGCCACCAAC
AGGGAGGCCCAGGGCGGGCAGAGGCTGCTGCTCTCCACGATGCATGAGGAGTACGAGTTT
GTGTCACCCAGCAGTGTGGCCATCGCTGAGCTGGTGGCCCTGTTCCTGGAGGGCCTGAAG
GAGAGGTCCATTTTCGCCATGGCCCTGCAGGACAGGAAGGCCACAGATGACACCACCCTC
CTGGCCTTCAAGAAGGGGGACCTGTTGGTCCTCACAAAGAAGCAGGGGCTGCTGGCCTCT
GAGAACTGGACCCTCGGCCAGAACGACAGGACAGGCAAGACGGGGCTGGTGCCCATGGCC
TGCCTCTACACCATCCCCACGGTCACTAAGCCCTCGGCACAGCTGCTGAGCTTGCTTGCC
ATGTCACCAGAGAAGAGGAAGCTGGCGGCTCAGGAGGGGCAGTTCACAGAGCCACGTCCT
GAGGAGCCACCCAAGGAAAAGCTGCACACCCTGGAGGAGTTCTCCTATGAGTTCTTCAGG
GCTCCAGAGAAGGACATGGTGAGCATGGCCGTGCTGCCCCTGGCCCGTGCCCGTGGCCAC
CTGTGGGCCTATTCCTGCGAGCCGCTGCGACAGCCGCTGCTCAAGCGAGTCCACGCCAAC
GTCGACCTCTGGGACATCGCCTGCCAGATCTTTGTCGCCATCCTCCGGTACATGGGCGAC
TACCCTTCTCGGCAGGCCTGGCCCACCCTGGAGCTCACCGACCAGATCTTCACACTGGCC
CTGCAGCACCCGGCCCTCCAGGACGAGGTCTACTGCCAGATCCTGAAGCAGCTGACGCAC
AACTCCAACAGGCACAGCGAAGAGCGGGGCTGGCAGCTGCTGTGGCTGTGCACGGGCCTC
TTCCCGCCCAGCAAGGGGCTGCTGCCCCATGCCCAGAAGTTTATAGACACTCGGAGGGGG
AAGCTGCTGGCCCCCGACTGCAGCCGCCGAATCCAGAAGGTCCTGAGGACGGGGCCCCGG
AAGCAGCCCCCGCACCAGGTGGAGGTGGAGGCCGCAGAGCAGAACGTCTCCCGCATCTGC
CACAAGATCTACTTCCCCAATGACACCAGTGAGATGCTGGAGGTGGTTGCCAACACACGG
GTGCGGGATGTGTGTGACAGCATTGCCACCAGGCTGCAGCTGGCCTCCTGGGAGGGCTGC
AGCCTCTTCATCAAGATTTCAGACAAGGTCATCAGCCAGAAGGAGGGAGACTTCTTCTTT
GATTCCTTGAGGGAGGTGTCTGACTGGGTGAAGAAGAACAAGCCCCAGAAAGAAGGGGCC
CCCGTGACGCTCCCCTACCAGGTGTACTTCATGCGGAAATTGTGGCTCAACATATCTCCA
GGGAAGGATGTGAATGCAGACACCATACTCCATTACCACCAGGAGCTGCCCAAGTACCTG
CGCGGATTCCACAAGTGTTCGCGGGAGGATGCCATCCACCTGGCGGGCCTCATCTACAAG
GCCCAGTTCAACAACGACCGGTCCCAGCTGGCTAGTGTCCCCAAGATCCTGAGGGAACTG
GTGCCTGAGAACCTCACACGCCTGATGTCCTCGGAGGAGTGGAAAAAGAGCATCCTTCTA
GCCTATGACAAGCATAAGGACAAGACAGTGGAGGAGGCCAAGGTGGCCTTCCTGAAGTGG
ATCTGCCGGTGGCCCACCTTCGGATCCGCCTTCTTCGAGGTGAAGCAAACCTCGGAGCCT
TCCTACCCGGACGTCATCCTCATCGCCATCAACCGACATGGGGTTCTGCTCATCCACCCC
AAGACCAAGGACCTGCTCACCACCTATCCCTTCACCAAGATCTCCAGCTGGAGCAGCGGC
AGCACCTACTTCCACATGGCGCTGGGGAGCCTGGGCCGTGGCAGCCGCCTGCTGTGCGAG
ACCTCCCTGGGCTATAAGATGGATGACCTGCTGACCTCATATGTGCAGCAGCTCCTGAGT
GCCATGAACAAGCAGCGGGGCTCCAAGGCCCCAGCCCTGGCCAGCACCTTG
Protein sequence predicted based on nucleotide sequence
MSGFRLGDHVWLEPPSTHKTGVAIGGIIKEAKPGKVLVEDDEGKEHWIRAEDFGVLSPMH PNSVQGVDDMIRLGDLNEAGMVHNLLIRYQQHKIYTYTGSILVAVNPFQVLPLYTLEQVQ LYYSRHMGELPPHVFAIANNCYFSMKRNKRDQCCIISGESGAGKTETTKLILQFLATISG QHSWIEQQVLEANPILEAFGNAKTIRNDNSSRFGKYIDIYFNPSGVIEGARIEQFLLEKS RVCRQAPEERNYHIFYCMLMGVSAEDKQLLSLGTPSEYHYLTMGNCTSCEGLNDAKDYAH IRSAMKILQFSDSESWDVIKLLAAILHLGNVGFMASVFENLDASDVMETPAFPTVMKLLE VQHQELRDCLIKHTILIRGEFVTRSLNIAQAADRRDAFVKGIYGHLFLWIVKKINAAIFT PPAQDPKNVRRAIGLLDIFGFENFENNSFEQLCINFANEHLQQFFVQHVFTMEQEEYRSE NISWDYIHYTDNRPTLDLLALKPMSIISLLDEESRFPQGTDLTMLQKLNSVHANNKAFLQ PKNIHDARFGIAHFAGEVYYQAEGFLEKNRDVLSTDILTLVYSSKNKFLREIFNLELAET KLGHGTIRQAKAGNHLFKSADSNKRPSTLGSQFKQSLDQLMKILTNCQPYFIRCIKPNEY KKPLLFDRELCLRQLRYSGMMETVHIRKSGFPIRYTFEEFSQRFGVLLPNAMRMQLQGKL RQMTLGITDVWLRTDKDWKAGKTKIFLRDHQDTLLEVQRSQVLDRAALSIQKVLRGYRYR KEFLRQRRAAVTLQAWWRGYCNRRNFKLILVGFERLQAIARSQPLARQYQAMRQRTVQLQ ALCRGYLVRQQVQAKRRAVVVIQAHARGMAARRNFQQRKANAPLVIPAEGQKSQGALPAK KRRSIYDTVTDTEMVEKVFGFLPAMIGGQEGQASPHFEDLESKTQKLLEVDLDTVPMAEE PEEDVDGLAEYTFPKFAVTYFQKSASHTHIRRPLRYPLLYHEDDTDCLAALVIWNVILRF MGDLPEPVLYARSSQQGSSVMRQIHDTLGREHGAQVPQHSRSAQVASQLNIGEEALEPDG LGADRPMSNLEKVHFIVGYAILRPSLRDEIYCQICKQLSENFKTSSLARGWILLSLCLGC FPPSERFMKYLLNFIGQGPATYGPFCAERLRRTYANGVRAEPPTWLELQAVKSKKHIPIQ VILATGESLTVPVDSASTSREMCMHIAHKQGLSDHLGFSLQVAVYDKFWSLGSGRDHMMD AIARCEQMAQERGESQRQSPWRIYFRKEFFTPWHDSREDPVSTELIYRQVLRGVWSGEYS FEKEEELVELLARHCYVQLGASAESKAVQELLPSCIPHKLYRTKPPDRWASLVTAACAKA PYTQKQVTPLAVREQVVDAARLQWPLLFSRLFEVITLSGPRLPKTQLILAVNWKGLCFLD QQEKMLLELSFPEVMGLATNREAQGGQRLLLSTMHEEYEFVSPSSVAIAELVALFLEGLK ERSIFAMALQDRKATDDTTLLAFKKGDLLVLTKKQGLLASENWTLGQNDRTGKTGLVPMA CLYTIPTVTKPSAQLLSLLAMSPEKRKLAAQEGQFTEPRPEEPPKEKLHTLEEFSYEFFR APEKDMVSMAVLPLARARGHLWAYSCEPLRQPLLKRVHANVDLWDIACQIFVAILRYMGD YPSRQAWPTLELTDQIFTLALQHPALQDEVYCQILKQLTHNSNRHSEERGWQLLWLCTGL FPPSKGLLPHAQKFIDTRRGKLLAPDCSRRIQKVLRTGPRKQPPHQVEVEAAEQNVSRIC HKIYFPNDTSEMLEVVANTRVRDVCDSIATRLQLASWEGCSLFIKISDKVISQKEGDFFF DSLREVSDWVKKNKPQKEGAPVTLPYQVYFMRKLWLNISPGKDVNADTILHYHQELPKYL RGFHKCSREDAIHLAGLIYKAQFNNDRSQLASVPKILRELVPENLTRLMSSEEWKKSILL AYDKHKDKTVEEAKVAFLKWICRWPTFGSAFFEVKQTSEPSYPDVILIAINRHGVLLIHP KTKDLLTTYPFTKISSWSSGSTYFHMALGSLGRGSRLLCETSLGYKMDDLLTSYVQQLLS AMNKQRGSKAPALASTL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
