Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878347
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3480nts
Open reading frame: 1 to 3480
ATGTACCTTTCCATCTGTTGCTGCTTTCTTCTATGGGCCCCTGCCCTCACTCTCAAGAAC
CTCAACTACTCCGTGCCGGAGGAGCAAGGGGCCGGCACGGTGATCGGGAACATCGGCAGG
GATGCTCGACTGCAGCCTGGGCTTCCGCCTGCAGAGCGCGGCGGCGGAGGGCGCAGCAAG
TCGGGTAGCTACCGGGTGCTGGAGAACTCCGCACCGCACCTGCTGGACGTGGACGCAGAC
AGCGGGCTCCTCTACACCAAGCAGCGCATCGACCGCGAGTCCCTGTGCCGCCACAATGCC
AAGTGCCAGCTGTCCCTCGAGGTGTTCGCCAACGACAAGGAGATCTGCATGATCAAGGTA
GAGATCCAGGACATCAACGACAACGCGCCCTCCTTCTCCTCGGACCAGATCGAAATGGAC
ATCTCGGAGAACGCTGCTCCGGGCACCCGCTTCCCCCTCACCAGCGCACATGACCCCGAC
GCCGGCGAGAATGGGCTCCGCACCTACCTGCTCACGCGCGACGATCACGGCCTCTTTGGA
CTGGACGTTAAGTCCCGCGGCGACGGCACCAAGTTCCCAGAACTGGTCATCCAGAAGGCT
CTGGACCGCGAGCAACAGAATCACCATACGCTCGTGCTGACTGCCCTGGACGGTGGCGAG
CCTCCACGTTCCGCCACCGTACAGATCAACGTGAAGGTGATTGACTCCAACGACAACAGC
CCGGTCTTCGAGGCGCCATCCTACTTGGTGGAACTGCCCGAGAACGCTCCGCTGGGTACA
GTGGTCATCGATCTGAACGCCACCGACGCCGATGAAGGTCCCAATGGTGAAGTGCTCTAC
TCTTTCAGCAGCTACGTGCCTGACCGCGTGCGGGAGCTCTTCTCCATCGACCCCAAGACC
GGCCTAATCCGTGTGAAGGGCAATCTGGACTATGAGGAAAACGGGATGCTGGAGATTGAC
GTGCAGGCCCGAGACCTGGGGCCTAACCCTATCCCAGCCCACTGCAAAGTCACGGTCAAG
CTCATCGACCGCAACGACAATGCGCCGTCCATCGGTTTCGTCTCCGTGCGCCAGGGGGCG
CTGAGCGAGGCCGCCCCTCCCGGCACCGTCATCGCCCTGGTGCGGGTCACTGACCGGGAC
TCTGGCAAGAACGGACAGCTGCAGTGTCGGGTCCTAGGCGGAGGAGGGACGGGCGGCGGC
GGGGGCCTGGGCGGGCCCGGGGGTTCCGTCCCCTTCAAGCTTGAGGAGAACTACGACAAC
TTCTACACGGTGGTGACTGACCGCCCGCTGGACCGCGAGACACAAGACGAGTACAACGTG
ACCATCGTGGCGCGGGACGGGGGCTCTCCTCCCCTCAACTCCACCAAGTCGTTCGCGATC
AAGATTCTAGACGAGAACGACAACCCGCCTCGGTTCACCAAAGGGCTCTACGTGCTTCAG
GTGCACGAGAACAACATCCCGGGAGAGTACCTGGGCTCTGTGCTCGCCCAGGATCCCGAC
CTGGGCCAGAACGGCACCGTATCCTACTCTATCCTGCCCTCGCACATCGGCGACGTGTCT
ATCTACACCTATGTGTCTGTGAATCCCACGAACGGGGCCATCTACGCCCTGCGCTCCTTT
AACTTCGAGCAGACCAAGGCTTTTGAGTTCAAGGTGCTTGCTAAGGACTCGGGGGCGCCC
GCGCACTTGGAGAGCAACGCCACGGTGAGGGTGACAGTGCTAGACGTGAATGACAACGCG
CCAGTGATCGTGCTCCCCACGCTGCAGAACGACACCGCGGAGCTGCAGGTGCCGCGCAAC
GCTGGCCTGGGCTATCTGGTGAGCACTGTGCGCGCCCTAGACAGCGACTTCGGCGAGAGC
GGGCGTCTCACCTACGAGATCGTGGACGGCAACGACGACCACCTGTTTGAGATCGACCCG
TCCAGCGGCGAGATCCGCACGCTGCACCCTTTCTGGGAGGACGTGACGCCCGTGGTGGAG
CTGGTGGTGAAGGTGACCGACCACGGCAAGCCTACCCTGTCCGCAGTGGCCAAGCTCATC
ATCCGCTCGGTGAGCGGATCCCTTCCCGAGGGGGTACCACGGGTGAATGGCGAGCAGCAC
CACTGGGACATGTCGCTGCCGCTCATCGTGACTCTGAGCACTATCTCCATCATCCTCCTA
GCGGCCATGATCACCATCGCCGTCAAGTGCAAGCGCGAGAACAAGGAGATCCGCACTTAC
AACTGCCGCATCGCCGAGTACAGCCACCCGCAGCTGGGTGGGGGCAAGGGCAAGAAGAAG
AAGATCAACAAAAATGATATCATGCTGGTGCAGAGCGAAGTGGAGGAGAGGAACGCCATG
AACGTCATGAACGTGGTGAGCAGCCCCTCCCTGGCCACCTCCCCCATGTACTTCGACTAC
CAGACCCGCCTGCCCCTCAGCTCGCCCCGGTCGGAGGTGATGTATCTCAAACCGGCCTCC
AACAACCTGACTGTCCCTCAGGGGCACGCGGGCTGCCACACCAGCTTCACCGGACAAGGG
ACTAATGCAAGCGAGACCCCTGCCACTCGGATGTCCATAATTCAGACAGACAATTTTCCC
GCAGAGCCCAATTACATGGGCAGCAGGCAGCAGTTTGTTCAAAGTAGCTCCACGTTTAAG
GACCCAGAAAGAGCCAGCCTGAGAGACAGTGGGCACGGGGACAGTGATCAGGCTGACAGT
GACCAAGACACTAACAAAGGCTCCTGCTGTGACATGTCTGTTAGGGAGGCACTCAAGATG
AAAACTACTTCAACTAAAAGCCAACCACTTGAACAAGAACCAGAAGAGTGTGTTAATTGC
ACAGATGAATGCCGAGTGCTTGGTCATTCTGACAGGTGCTGGATGCCACAGTTCCCTGCA
GCCAATCAGGCTGAAAATGCAGATTACCGCACAAATCTCTTTGTACCTACAGTTGAAGCT
AATGTTGAGACTGAGACTTACGAAACTGTGAATCCCACTGGGAAAAAGACTTTTTGTACA
TTTGGAAAAGACAAGCGAGAGCACACTATTCTCATTGCCAACGTTAAACCTTATTTAAAA
GCCAAACGTGCCCTGAGCCCTCTCCTCCAAGAGGTCCCCTCAGCATCAAGCAGCCCAACC
AAGGCGTGCATCGAGCCTTGCACCTCAACAAAAGGCTCCCTGGATGGCTGTGAAGCAAAA
CCAGGAGCCCTGGCTGAAGCAAGCAGTCAGTACTTGCCCACTGACAGTCAATATCTGTCA
CCTAGTAAGCAACCAAGAGACCCTCCCTTCATGGCTTCCGATCAGATGGCAAGGGTCTTT
GCAGATGTGCATTCCAGAGCCAGCCGGGATTCCAGTGAGATGGGTGCTGTTCTTGAGCAG
CTTGACCACCCCAACAGGGATCTGGGCAGAGAGTCTGTGGATGCAGAGGAAGTTGTGAGA
GAAATTGATAAGCTTTTGCAAGACTGCCGGGGAAACGACCCTGTGGCTGTGAGAAAGTTG
Protein sequence predicted based on nucleotide sequence
MYLSICCCFLLWAPALTLKNLNYSVPEEQGAGTVIGNIGRDARLQPGLPPAERGGGGRSK SGSYRVLENSAPHLLDVDADSGLLYTKQRIDRESLCRHNAKCQLSLEVFANDKEICMIKV EIQDINDNAPSFSSDQIEMDISENAAPGTRFPLTSAHDPDAGENGLRTYLLTRDDHGLFG LDVKSRGDGTKFPELVIQKALDREQQNHHTLVLTALDGGEPPRSATVQINVKVIDSNDNS PVFEAPSYLVELPENAPLGTVVIDLNATDADEGPNGEVLYSFSSYVPDRVRELFSIDPKT GLIRVKGNLDYEENGMLEIDVQARDLGPNPIPAHCKVTVKLIDRNDNAPSIGFVSVRQGA LSEAAPPGTVIALVRVTDRDSGKNGQLQCRVLGGGGTGGGGGLGGPGGSVPFKLEENYDN FYTVVTDRPLDRETQDEYNVTIVARDGGSPPLNSTKSFAIKILDENDNPPRFTKGLYVLQ VHENNIPGEYLGSVLAQDPDLGQNGTVSYSILPSHIGDVSIYTYVSVNPTNGAIYALRSF NFEQTKAFEFKVLAKDSGAPAHLESNATVRVTVLDVNDNAPVIVLPTLQNDTAELQVPRN AGLGYLVSTVRALDSDFGESGRLTYEIVDGNDDHLFEIDPSSGEIRTLHPFWEDVTPVVE LVVKVTDHGKPTLSAVAKLIIRSVSGSLPEGVPRVNGEQHHWDMSLPLIVTLSTISIILL AAMITIAVKCKRENKEIRTYNCRIAEYSHPQLGGGKGKKKKINKNDIMLVQSEVEERNAM NVMNVVSSPSLATSPMYFDYQTRLPLSSPRSEVMYLKPASNNLTVPQGHAGCHTSFTGQG TNASETPATRMSIIQTDNFPAEPNYMGSRQQFVQSSSTFKDPERASLRDSGHGDSDQADS DQDTNKGSCCDMSVREALKMKTTSTKSQPLEQEPEECVNCTDECRVLGHSDRCWMPQFPA ANQAENADYRTNLFVPTVEANVETETYETVNPTGKKTFCTFGKDKREHTILIANVKPYLK AKRALSPLLQEVPSASSSPTKACIEPCTSTKGSLDGCEAKPGALAEASSQYLPTDSQYLS PSKQPRDPPFMASDQMARVFADVHSRASRDSSEMGAVLEQLDHPNRDLGRESVDAEEVVR EIDKLLQDCRGNDPVAVRKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
