Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878363
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6093nts
Open reading frame: 1 to 6093
ATGTGGACCCCCACGGAGGAGGAGAAATACGGCGTAGTGATATGCAGCTTTCGAGGATCT
GTCCCTCAAGGGTTGGTCTTAGAAATAGGAGAAACAGTCCAGATTCTTGAAAAATGTGAA
GGTTGGTACAGAGGAGTTTCAACAAAGAAGCCAAATGTGAAGGGGATCTTTCCTGCAAAT
TACATTCACTTGAAAAAGGCAATTGTCAGTAATAGGGGGCAGTATGAAACTGTGGTTCCA
CTTGAAGATTCTATTGTGACTGAGGTTACAGCAACTCTACAAGAATGGGCAAGTTTGTGG
AAACAGTTGTATGTGAAACACAAAGTAGATCTTTTCTACAAACTACGCCATGTGATGAAT
GAACTTATTGACCTGCGAAGGCAGCTACTGTCTGGTCACCTGACTCAGGATCAGGTGCGG
GAGGTTAAGCGGCACATCACCGTGCGCCTGGACTGGGGTAATGAACATTTGGGCCTGGAC
CTGGTGCCTCGGAAGGACTTTGAAGTAGTGGACTCGGACCAGATTAGTGTCTCAGATCTC
TATAAGATGCATTTATCTAGCCGGCAGAGTGTACAGCAAAGCACATCCCAGGTAGATACA
ATGCGCCCACGTCATGGGGAAACATGTCGGATGCCAGTGCCACATCACTTCTTCCTCAGC
CTGAAGAGTTTCACTTACAATACTATTGGGGAAGATACCGATGTCTTCTTTTCCTTATAT
GACATGAGGGAAGGCAAGCAGATCAGTGAGCGGTTTCTGGTAAGACTGAACAAGAATGGT
GGGCCGAGGAACCCAGAGAAGATAGAACGAATGTGTGCCCTTTTTACAGATCTGAGCAGC
AAAGATATGAAGAGAGATTTGTATATCGTTGCCCATGTGATCCGAATAGGCCGGATGCTC
CTGAACGACTCAAAGAAAGGTCCTCCTCACCTGCACTACAGGCGACCATATGGCTGTGCG
GTCCTAAGCATCTTGGATGTCCTACAGTCACTCACAGAAGTAAAGGAAGAAAAGGATTTT
GTTCTTAAGGTTTACACGTGCAACAACGAGAGTGAGTGGTCCCAGATCCACGAGAACATC
ATCCGAAAGTCCAGTGCCAAGTACTCTGCCCCCAGCGCCAGCCATGGTCTTATCATTTCT
CTGCAGCTTCTTCGTGGAGACATGGAACAGATTCGGAGAGAAAATCCCATGATATTTAAT
AGGGGATTGGCAATTACAAGAAAATTGGGATTTCCTGATGTCATTATGCCAGGTGATATC
CGCAATGACCTGTACCTAACCCTGGAGAAGGGGGATTTCGAGAGAGGAGGAAAGAGTGTA
CAAAAGAATATTGAAGTGACCATGTATGTGCTTTATGCAGATGGAGAAATCTTGAAGGAT
TGCATCAGCTTGGGTTCAGGAGAGCCAAATAGGAGTTCCTACCACTCCTTTGTCCTCTAC
CACAGTAATAGTCCTCGCTGGGGAGAAATTATCAAATTGCCTATCCCCATTGACCGGTTC
CGGGGCTCCCACCTGCGCTTTGAGTTCAGACATTGTTCCACAAAGGACAAAGGGGAAAAG
AAACTCTTTGGCTTTGCATTCTCAACCCTGATGCGTGATGATGGCACCACCCTCTCAGAT
GATATTCACGAGCTTTATGTGTACAAGTGTGATGAGAATAGCACGTTTAATAACCATGCT
CTGTACCTGGGCCTGCCCTGCTGCAAAGAGGACTACAATGGCTGCCCTAATATTCCTTCT
AGCCTCATCTTCCAGCGCAGCACCAAAGAGTCTTTCTTCATCTCCACTCAGCTCTCCTCT
ACCAAACTCACCCAGAATGTGGACCTCCTAGCTCTGCTGAAGTGGAAAGCCTTCCCCGAC
CGGATCATGGATGTACTAGGGCGGCTGCGGCATGTCAGTGGGGAGGAAATTGTTAAGTTT
CTGCAGGACATCTTAGATACACTCTTTGTGATTTTGGATGATAATACAGAGAAGTACGGC
CTGTTGGTTTTTCAGTCTCTGGTGTTCATCATCAACCTGCTCCGAGACATCAAGTATTTT
CACTTTCGACCTGTGATGGACACGTATATCCAGAAGCACTTTGCTGGAGCTCTGGCATAC
AAGGAGCTCATCCGCTGTTTGAAGTGGTATATGGACTGCTCAGCAGAACTGATTCGACAG
GACCACATTCAAGAAGCTATGCGGGCCTTGGAGTACCTTTTCAAGTTCATTGTACAGTCA
CGGATCCTGTACTCACGAGCCACTTGTGGAATGGAAGAGGAACAATTCAGATCCAGTATC
CAAGAACTTTTCCAGTCCATCCGGTTTGTGCTCAGTCTGGACAGCCGAAACTCAGAAACA
CTCCTTTTTACTCAGGCTGCACTCCTCAATTCTTTCCCAACCATCTTTGATGAGCTTCTG
CAAATGTTCACCGTGCAAGAGGTGGCAGAGTTTGTGAGAGGGACACTGGGGAGCATGCCC
AGCACTGTGCACATTGGGCAGTCAATGGACGTGGTCAAGCTGCAGTCCATTGCCAGGACA
GTGGATAGCCGCCTGTTTTCTTTCTCAGAATCCCGCCGCATCCTGCTTCCTGTGGTTCTC
CATCACATTCACCTTCACCTGAGGCAGCAGAAAGAGCTGCTAATTTGCTCAGGGATTCTT
GGCAGCATCTTCTCCATCGTCAAGACCAGCTCTCTGGAGGCAGATGTCATGGAGGAAGTA
GAGATGATGGTGGAGAGCCTCCTGGACGTGCTCTTGCAGACTCTGCTCACCATCATGAGC
AAATCGCACGCTCAGGAGGCGGTAAGAGGGCAGCGGTGCCCGCAGTGCACAGCCGAGATC
ACTGGCGAGTATGTGTCCTGCCTTCTCTCACTGCTCCGCCAGATGTGTGACACCCATTTC
CAGCACCTCCTGGACAACTTCCAGAGCAAAGATGAACTCAAGGAATTTCTGCTGAAGATT
TTTTGCGTGTTCCGGAACCTGATGAAGATGAGTGTCTTCCCTCGGGACTGGATGGTAATG
AGACTGCTCACAAGCAATATTATAGTCACTACTGTCCAGTACCTGTCCTCTGCACTGCAC
AAGAATTTCACAGAGACTGACTTTGACTTTAAGGTGTGGAATTCTTACTTTAGCCTGGCA
GTTCTATTCATAAATCAGCCAAGCCTTCAGCTAGAAATTATCACCTCAGCCAAAAGGAAG
AAGATTCTAGATAAGTATGGGGACATGCGTGTAATGATGGCCTATGAACTGTTCAGCATG
TGGCAGAATTTGGGTGAACATAAGATCCACTTTATTCCGGGAATGATTGGTCCTTTTCTG
GGTGTGACACTGGTCCCACAGCCAGAAGTACGGAATATCATGATTCCCATCTTTCATGAC
ATGATGGACTGGGAGCAGAGAAAAAATGGCAACTTCAAACAGGTGGAGGCCGAGTTGATT
GACAAGCTGGACAGCATGGTGTCAGAAGGGAAAGGTGACGAGAGCTACAGGGAGCTCTTC
AGCCTACTAACCCAGCTGTTTGGGCCCTACCCCAGCCTGCTGGAGAAGGTTGAACAAGAA
ACATGGCGCGAGACCGGCATTTCCTTTGTGACCTCAGTCACCCGCCTCATGGAACGTCTT
CTTGACTACAGGGACTGCATGAAAGGAGAGGAAACAGAGAATAAGAAGATAGGCTGCACT
GTTAACCTGATGAATTTTTACAAATCTGAGATTAACAAGGAAGAAATGTATATCCGCTAC
ATCCATAAGCTTTGTGACATGCACTTGCAGGCCGAAAACTACACAGAGGCCGCATTTACC
CTGCTCCTTTACTGTGAGCTGCTGCAGTGGGAGGACCGGCCACTACGGGAATTCCTCCAC
TACCCATCGCAGACAGAGTGGCAGCGGAAGGAGGGACTGTGCCGGAAGATCATTCACTAC
TTCAACAAAGGCAAGAGCTGGGAGTTTGGGATCCCACTGTGCAGGGAGCTGGCGTGTCAG
TACGAGAGCCTCTATGATTACCAGAGCCTCAGCTGGATTCGGAAAATGGAGGCCAGCTAC
TATGACAACATTATGGAGCAGCAACGCCTGGAGCCTGAGTTCTTTCGGGTCGGCTTCTAT
GGCAGGAAGTTTCCTTTCTTTCTTCGGAACAAAGAATACGTGTGCCGTGGCCATGACTAC
GAGAGGCTGGAGGCCTTCCAGCAGAGGATGCTCAGTGAGTTTCCGCAGGCTGTCGCCATG
CAGCACCCCAACCATCCTGATGACGCCATCCTACAGTGCGATGCCCAGTACTTGCAGATC
TATGCAGTGACGCCCATTCCAGATTATGTGGATGTTCTGCAGATGGATAGGGTACCAGAT
CGAGTCAAGAGCTTCTATCGCGTCAACAATGTGAGGAAGTTCCGGTATGACAGGCCTTTT
CACAAAGGCCCCAAGGACAAGGAGAATGAATTCAAGAGCCTGTGGATTGAACGTACCACA
CTGACCCTGACCCACAGCTTGCCTGGCATCTCTCGGTGGTTTGAAGTGGAGAGGAGGGAA
CTGGTGGAGGTGAGCCCTCTGGAGAATGCCATCCAAGTGGTTGAGAATAAGAACCAGGAG
CTACGCTCCCTGATCAGCCAGTATCAACACAAGCAGGTGCATGGCAACATTAACCTGCTA
AGCATGTGCCTGAATGGTGTCATTGATGCAGCTGTCAATGGAGGCATTGCACGCTATCAG
GAGGCCTTCTTTGATAAAGATTACATCAACAAGCACCCAGGAGATGCTGAGAAGATCACC
CAGCTCAAGGAGCTTATGCAGGAGCAGGTTCATGTCCTTGGAGTTGGGCTAGCAGTTCAT
GAGAAGTTTGTGCACCCAGAAATGCGGCCTCTGCATAAGAAGCTAATTGATCAGTTCCAG
ATGATGCGGGCCAGTCTCTACCATGAGTTTCCAGGTTTGGATAAGCTAAGTCCTGCATGT
TCAGGCACCAGCACCCCACGGGGAAATGTTCTGGCATCCCATAGCCCCATGAGTCCGGAG
AGCATCAAGATGACCCACCGGCACAGCCCCATGAACTTGATGGGCACAGGCCGCCATTCA
TCATCCTCTCTCTCCTCACATGCGTCTAGTGAAGCAGGAAACATGGTGATGCTGGGTGAC
GGCTCCATGGGTGATGCTCCTGAGGACCTGTACCACCACATGCAGCTCGCGTATCCCAAC
CCCAGGTACCAAGGCTCAGTCACCAACGTCTCTGTTCTGTCCTCGTCCCAGGCAAGCCCT
TCTTCCTCCAGCCTGAGTTCCACTCACTCAGCACCATCCCAGATGATTACCTCTGCCCCT
TCCAGTGCCCGAGGCTCTCCCTCTCTGCCAGATAAGTACCGCCATGCCCGTGAAATGATG
TTGTTGCTGCCCACATACCGGGACCGCCCAAGCAGTGCCATGTATCCAGCAGCCATCCTG
GAGAACGGACAGCCGCCGAATTTCCAGCGAGCCCTGTTCCAGCAAGTGGTCGGAGCCTGC
AAACCCTGCAGTGATCCCAATCTGTCTGTGGCTGAAAAAGGTCATTACTCCCTACACTTT
GACGCCTTCCACCACCCTCTGGGTGATACCCCCCCAGCCCTCCCTGCCCGGACCCTGCGC
AAGTCTCCTCTCCACCCTATCCCAGCCTCCCCCACAAGCCCCCAGTCAGGTCTGGACGGC
AGCAACTCTACGCTGTCCGGCAGTGCCAGCAGCGGCGTGTCCTCCTTGAGTGAGAGTAAC
TTTGGGCACTCCTCGGAGGCCCCACCTCGCACTGACACCATGGACTCCATGCCAAGTCAG
GCCTGGAATGCTGACGAAGATCTTGAGCCACCCTACCTCCCTGTCCACTACAGCCTCTCT
GAGTCTGCCGTCCTGGACTCCATCAAGGCCCAGCCATGCCGAAGCCACTCAGCCCCAGGG
TGCGTCATCCCTCAGGACCCCATGGACCCGCCTGCGCTGCCGCCCAAGCCCTACCACCCC
CGCCTGCCGGCCCTGGAGCACGATGAGGGGGTGCTGCTGCGTGAAGAGACTGAGAGGCCT
CGAGGCCTGCACCGCAAGGCTCCATTGCCTCCTGGGAGCGCTAAGGAGGAGCAGGCCCGC
ATGGCCTGGGAGCACGGCCGAGGGGAGCAGTTG
Protein sequence predicted based on nucleotide sequence
MWTPTEEEKYGVVICSFRGSVPQGLVLEIGETVQILEKCEGWYRGVSTKKPNVKGIFPAN YIHLKKAIVSNRGQYETVVPLEDSIVTEVTATLQEWASLWKQLYVKHKVDLFYKLRHVMN ELIDLRRQLLSGHLTQDQVREVKRHITVRLDWGNEHLGLDLVPRKDFEVVDSDQISVSDL YKMHLSSRQSVQQSTSQVDTMRPRHGETCRMPVPHHFFLSLKSFTYNTIGEDTDVFFSLY DMREGKQISERFLVRLNKNGGPRNPEKIERMCALFTDLSSKDMKRDLYIVAHVIRIGRML LNDSKKGPPHLHYRRPYGCAVLSILDVLQSLTEVKEEKDFVLKVYTCNNESEWSQIHENI IRKSSAKYSAPSASHGLIISLQLLRGDMEQIRRENPMIFNRGLAITRKLGFPDVIMPGDI RNDLYLTLEKGDFERGGKSVQKNIEVTMYVLYADGEILKDCISLGSGEPNRSSYHSFVLY HSNSPRWGEIIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLFGFAFSTLMRDDGTTLSD DIHELYVYKCDENSTFNNHALYLGLPCCKEDYNGCPNIPSSLIFQRSTKESFFISTQLSS TKLTQNVDLLALLKWKAFPDRIMDVLGRLRHVSGEEIVKFLQDILDTLFVILDDNTEKYG LLVFQSLVFIINLLRDIKYFHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDCSAELIRQ DHIQEAMRALEYLFKFIVQSRILYSRATCGMEEEQFRSSIQELFQSIRFVLSLDSRNSET LLFTQAALLNSFPTIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIART VDSRLFSFSESRRILLPVVLHHIHLHLRQQKELLICSGILGSIFSIVKTSSLEADVMEEV EMMVESLLDVLLQTLLTIMSKSHAQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMCDTHF QHLLDNFQSKDELKEFLLKIFCVFRNLMKMSVFPRDWMVMRLLTSNIIVTTVQYLSSALH KNFTETDFDFKVWNSYFSLAVLFINQPSLQLEIITSAKRKKILDKYGDMRVMMAYELFSM WQNLGEHKIHFIPGMIGPFLGVTLVPQPEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELI DKLDSMVSEGKGDESYRELFSLLTQLFGPYPSLLEKVEQETWRETGISFVTSVTRLMERL LDYRDCMKGEETENKKIGCTVNLMNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFT LLLYCELLQWEDRPLREFLHYPSQTEWQRKEGLCRKIIHYFNKGKSWEFGIPLCRELACQ YESLYDYQSLSWIRKMEASYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEYVCRGHDY ERLEAFQQRMLSEFPQAVAMQHPNHPDDAILQCDAQYLQIYAVTPIPDYVDVLQMDRVPD RVKSFYRVNNVRKFRYDRPFHKGPKDKENEFKSLWIERTTLTLTHSLPGISRWFEVERRE LVEVSPLENAIQVVENKNQELRSLISQYQHKQVHGNINLLSMCLNGVIDAAVNGGIARYQ EAFFDKDYINKHPGDAEKITQLKELMQEQVHVLGVGLAVHEKFVHPEMRPLHKKLIDQFQ MMRASLYHEFPGLDKLSPACSGTSTPRGNVLASHSPMSPESIKMTHRHSPMNLMGTGRHS SSSLSSHASSEAGNMVMLGDGSMGDAPEDLYHHMQLAYPNPRYQGSVTNVSVLSSSQASP SSSSLSSTHSAPSQMITSAPSSARGSPSLPDKYRHAREMMLLLPTYRDRPSSAMYPAAIL ENGQPPNFQRALFQQVVGACKPCSDPNLSVAEKGHYSLHFDAFHHPLGDTPPALPARTLR KSPLHPIPASPTSPQSGLDGSNSTLSGSASSGVSSLSESNFGHSSEAPPRTDTMDSMPSQ AWNADEDLEPPYLPVHYSLSESAVLDSIKAQPCRSHSAPGCVIPQDPMDPPALPPKPYHP RLPALEHDEGVLLREETERPRGLHRKAPLPPGSAKEEQARMAWEHGRGEQL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
