Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878373
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6144nts
Open reading frame: 1 to 6144
ATGGCTGCCTCCGAGCGCCGCGCCTTCGCGCACAAGATCAACAGGACGGTGGCCGCAGAG
GTGCGGAAGCAGGTGTCCCGGGAACGCAGTGGCTCCCCCCACTCCAGCAGGCGCTGCAGC
AGCTCCCTGGGGGTCCCACTGACTGAAGTTGTCGAGCCCCTGGACTTTGAGGATGTACTT
CTGAGCCGGCCACCAGATGCTGAGCCCGGGCCCCTCAGGGACCTGGTAGAATTCCCAGCT
GATGACTTGGAGCTGCTGCTGCAGCCCCGGGAATGCCGGACCACGGAGCCCGGGATCCCC
AAGGATGAAAAACTGGATGCCCAGGTGAGGGCCGCGGTGGAGATGTATATTGAGGACTGG
GTCATTGTCCACAGAAGGTATCAGTACCTGAGTGCAGCATACAGCCCCGTCACCACAGAC
ACACAGCGGGAGCGACAGAAGGGCCTCCCCCGCCAGGTCTTTGAGCAGGATGCTTCTGGA
GACGAGAGGTCCGGCCCTGAGGACTCGAATGACTCCCGGCGTGGCTCGGGCTCCCCGGAA
GACACCCCTCGAAGCAGTGGTGCCTCTAGCATCTTCGACCTGAGGAACCTGGCAGCTGAC
TCATTGCTGCCCTCTCTGCTAGAGCGGGCGGCCCCAGAAGATGTGGACCGGCGCAATGAA
ACCCTTCGACGGCAGCACCGGCCCCCGGCCCTGCTCACCCTCTACCCGGCACCTGACGAG
GATGAAGCCGTGGAACGCTGTAGCCGCCCAGAGCCACCCCGCGAGCACTTTGGACAAAGG
ATCTTGGTCAAGTGTCTGTCGCTCAAGTTCGAGATTGAAATTGAGCCCATCTTTGGGATC
TTGGCTCTGTATGATGTGCGGGAGAAAAAGAAGATCTCGGAGAACTTCTACTTCGACCTG
AACTCGGACTCCATGAAGGGGCTGCTTCGGGCTCATGGCACCCACCCTGCCATCTCCACC
CTGGCCCGCTCTGCCATCTTCTCTGTGACCTACCCCTCACCTGACATCTTCCTGGTCATC
AAGTTGGAGAAGGTGCTTCAGCAAGGGGACATCAGTGAGTGCTGTGAGCCTTACATGGTG
TTGAAAGAAGTGGACACAGCCAAGAACAAAGAGAAGCTAGAGAAGCTGCGCCTGGCGGCC
GAGCAGTTCTGCACCCGCCTGGGCCGCTACCGCATGCCCTTCGCCTGGACGGCCGTGCAC
TTGGCCAACATCGTGAGCAGCGCTGGGCAGCTGGACCGGGACTCTGACTCGGAGGGCGAG
CGCCGGCCAGCCTGGACAGACCGCCGCCGTCGGGGGCCCCAGGACCGGGCGAGTAGTGGG
GACGACGCCTGCAGCTTCTCTGGCTTCCGTCCAGCCACGCTAACTGTCACAAACTTCTTT
AAGCAGGAGGCTGAGCGACTCAGTGACGAGGACCTCTTCAAGTTCCTGGCTGACATGAGG
CGCCCGTCGTCCCTGCTGCGGCGACTACGTCCTGTGACTGCCCAGCTCAAGATCGACATT
TCTCCGGCTCCTGAAAATCCCCACTTCTGCCTCTCCCCTGAGCTGCTTCATATCAAGCCC
TACCCGGACCCCAGGGGCCGGCCCACCAAGGAGATTCTGGAGTTCCCCGCCCGCGAAGTC
TATGCCCCCCATACCAGCTACAGGAACCTGCTGTACGTGTACCCGCACAGCCTCAACTTC
AGCAGCCGCCAGGGCTCCGTGCGCAACCTTGCTGTGCGAGTGCAGTACATGACAGGCGAG
GACCCCAGCCAGGCTCTGCCGGTCATCTTTGGCAAGTCCAGCTGCAGTGAATTTACCCGC
GAGGCCTTCACACCGGTGGTCTACCATAACAAGTCCCCCGAGTTCTACGAGGAGTTCAAG
CTGCATCTTCCAGCCTGCGTGACAGAGAACCATCACCTGCTGTTCACCTTCTACCATGTC
AGCTGCCAGCCCCGGCCGGGCACTGCCCTGGAGACACCCGTGGGCTTTACTTGGATCCCA
CTGCTGCAGCACGGGCGCCTGAGGACCGGCCCCTTCTGTCTCCCAGTGTCTGTGGACCAG
CCGCCGCCCAGCTATTCCGTGCTCACACCCGATGTGGCGCTTCCGGGCATGCGCTGGGTG
GACGGTCACAAGGGCGTGTTCAGTGTGGAGCTCACAGCCGTGTCCTCTGTGCACCCCCAG
GACCCCTACCTGGACAAATTCTTCACCCTGGTGCACGTCCTGGAGGAGGGAGCCTTCCCA
TTCCGGCTCAAGGACACTGTGCTGAGCGAGGGCAACGTGGAGCAGGAGCTGCGGGCCAGT
CTTGCAGCACTGCGCCTGGCCAGCCCCGAACCCCTTGTGGCCTTCTCCCACCACGTGCTG
GACAAGCTCGTGCGTCTGGTCATCAGGCCCCCGATCATCAGTGGCCAGATTGTGAACCTG
GGCCGTGGAGCCTTTGAAGCAATGGCCCATGTAGTCAGCCTTGTTCACCGGAGCCTGGAG
GCAGCCCAGGATGCCCGCGGTCACTGCCCACAGCTGGCTGCCTACGTCCACTACGCCTTT
CGCCTTCCTGGCACTGAGCCCAGCCTCCCGGATGGGGCCCCTCCAGTGACAGTGCAGGCT
GCCACACTGGCCCGTGGCTCTGGTCGCCCCGCAAGCCTCTACCTGGCGCGTTCCAAGAGC
ATCAGCAGCAGCAACCCTGACCTCGCCGTGGCCCCTGGCTCTGTGGATGACGAGGTTTCC
CGCATCCTGGCCAGCAAGCTGCTTCACGAGGAGCTGGCTCTGCAGTGGGTGGTCAGCAGC
AGTGCCGTACGCGAGGCCATCCTCCAGCACGCCTGGTTCTTCTTCCAGCTCATGGTGAAG
AGTATGGCGCTGCACCTGCTGCTTGGCCAGCGACTAGACACACCCCGCAAGCTGCGCTTC
CCCGGACGCTTCCTGGACGACATCACTGCCTTGGTGGGCTCTGTGGGCCTGGAGGTCATC
ACCCGTGTCCACAAGGATGTGGAGCTGGCCGAGCACCTCAACGCCAGCCTGGCTTTCTTC
CTCAGTGACCTTCTGTCCCTGGTGGACCGGGGCTTTGTCTTCAGCCTGGTCCGGGCCCAC
TACAAGCAGGTGGCCACGCGGCTCCAGTCGTCCCCTAATCCAGCAGCCCTGCTGACCCTG
CGCATGGAATTCACCCGCATCCTGTGCAGCCACGAGCACTACGTGACCCTCAACCTCCCC
TGCTGCCCCCTGTCACCTCCAGCCTCGCCCTCCCCCTCTGTGTCCTCCACCACCTCCCAG
AGCTCCACCTTCTCCAGCCAAGCCCCGGACCCCAAGGTGACCAGCATGTTCGAACTGAGT
GGACCATTCCGGCAGCAGCACTTCCTAGCTGGGCTCCTGCTGACGGAGCTGGCACTGGCC
CTCGAACCTGAGGCTGAAGGGGCATTCCTGTTGCACAAGAAGGCCATCAGTGCTGTGCAC
AGCCTGCTATGTGGCCATGACACTGACCCCCGCTACGCCGAGGCCACTGTGAAGGCTCGT
GTGGCCGAGCTGTACCTGCCACTGCTATCGATTGCACGGGATACCTTGCCACGGCTGCAT
GACTTTGCTGAGGGCCCAGGTCAGCGGTCAAGACTGGCCTCAATGCTTGACTCAGACACA
GAAGGCGAAGGGGACATTGCGGGTACCATCAACCCCTCTGTGGCCATGGCCATTGCTGGT
GGCCCCCTAGCCCCTGGCTCCCGGGCCAGCATCTCCCAGGGGCCACCAACGGCTTCTCGC
GCAGGCTGTGCCCTCTCTGCTGAGTCAAGCCGGACCTTGCTGGCGTGTGTGCTGTGGGTG
CTGAAAAACACCGAGCCGGCGCTCCTGCAGCGCTGGGCCACTGACCTGACACTCCCCCAG
CTGGGACGTCTGTTGGATTTGCTGTACCTTTGCCTAGCTGCCTTTGAGTACAAGGGGAAA
AAGGCCTTTGAACGCATCAACAGCCTCACATTCAAAAAATCTCTGGATATGAAGGCGCGG
CTAGAGGAAGCCATTCTGGGTACCATCGGAGCTCGACAAGAAATGGTTCGGCGAAGTCGT
GAGAGGAGCCCGTTTGGGAATCCGGAGAATGTGCGCTGGCGGAAGAGCGTCACACACTGG
AAGCAAACCTCAGACCGCGTGGACAAGACCAAGGATGAAATGGAACACGAGGCCTTGGTG
GAAGGGAACCTGGCAACCGAGGCAAGCCTAGTGGTTCTGGACACACTGGAGATCATCGTG
CAGACGGTGATGCTTTCAGAAGCCCGGGAGAGCGTCTTGGGGGCAGTGCTGAAGGTTGTG
CTGTACAGCCTGGGCAGTGCCCAGAGTGCCCTCTTCTTGCAGCATGGCCTGGCCACCCAG
AGGGCCCTTGTGTCCAAGTTCCCGGAGCTGCTGTTCGAGGAGGACACGGAGCTGTGTGCC
GACCTGTGCCTGAGGCTCCTACGACACTGTGGCAGCCGCATCAGCACCATCCGCACGCAC
GCCAGCGCCTCGCTGTACCTGCTCATGCGACAGAACTTCGAGATCGGCCACAACTTTGCC
CGTGTGAAGATGCAGGTCACCATGTCTCTCTCGTCCCTGGTGGGGACGACGCAGAACTTC
AGTGAAGAGCACCTGCGACGTTCACTCAAAACCATCCTCACCTATGCTGAGGAGGACATG
GGGCTGCGGGACAGCACCTTCGCAGAGCAGGTCCAGGACCTGATGTTCAACCTGCACATG
ATCCTGACGGACACGGTGAAGATGAAGGAACACCAGGAGGACCCTGAGATGCTCATCGAC
CTCATGTACAGAATTGCCCGGGGCTACCAGGGCTCACCGGACCTTCGGCTGACCTGGTTG
CAGAACATGGCCGGGAAGCACGCGGAGCTGGGCAACCACGCCGAGGCCGCCCAGTGCATG
GTGCACGCGGCCGCCCTCGTGGCTGAGTACCTCGCCCTGCTCGAGGACCACCGCCACCTG
CCCGTGGGCTGCGTTTCCTTCCAGAACATCTCATCCAACGTGCTAGAGGAGTCCGCCATC
TCCGACGACATCCTGTCGCCCGACGAGGAGGGCTTCTGCTCCGGGAAGCACTTCACTGAG
CTGGGGCTGGTAGGGTTGCTGGAACAGGCAGCCGGCTACTTCACCATGGGCGGGCTCTAC
GAGGCGGTGAATGAGGTCTACAAGAACCTCATCCCCATCCTGGAAGCCCACCGTGACTAC
AAGAAGCTGGCCGCGGTGCACGGCAAACTGCAGGAGGCCTTCACCAAGATCATGCACCAG
AGTTCCGGCTGGGAGCGCGTGTTCGGGACGTATTTCCGCGTGGGCTTCTACGGCGCCCAC
TTCGGTGACCTGGATGAGCAGGAGTTTGTGTACAAGGAGCCATCGATCACGAAGCTGGCA
GAGATCTCACACCGGCTGGAGGAGTTCTACACGGAGAGATTTGGCGACGACGTCGTTGAG
ATTATCAAAGACTCTAACCCTGTGGACAAGTCCAAGCTTGACTCACAAAAGGCCTACATC
CAGATCACGTATGTGGAACCGTACTTTGATACCTACGAGCTCAAGGACCGGGTGACCTAC
TTTGACCGCAACTATGGGCTTCGCACATTCCTGTTCTGCACGCCGTTCACGCCGGATGGG
CGCGCACACGGGGAGCTGCCCGAGCAACACAAGCGTAAGACGCTGCTCAGCACCGACCAC
GCCTTCCCCTACATCAAGACTCGCATCCGTGTGTGCCACCGGGAGGAGACGGTGCTGACG
CCAGTGGAGGTGGCCATCGAGGACATGCAGAAGAAGACACGGGAGCTGGCCTTTGCCACC
GAGCAGGACCCACCAGATGCTAAGATGCTACAGATGGTGCTTCAGGGCTCTGTAGGGCCC
ACCGTGAACCAGGGTCCCCTGGAGGTGGCCCAGGTGTTTTTAGCAGAGATCCCGGAAGAC
CCCAAGCTCTTCCGGCATCACAACAAATTGCGGCTCTGCTTCAAGGACTTCTGCAAGAAA
TGTGAGGATGCGCTGCGGAAAAATAAGGCCCTGATTGGGCCGGACCAGAAGGAGTACCAC
CGTGAGCTGGAGCGCAACTACTGCCGCCTGCGGGAGGCTCTGCAGCCCCTGCTTACCCAG
CGCCTGCCCCAGCTGATGGCACCCACCCCACCCGGCCTCAGGAACTCCTTGAACAGAGCA
AGTTTCCGAAAGGCAGACCTCTTG
Protein sequence predicted based on nucleotide sequence
MAASERRAFAHKINRTVAAEVRKQVSRERSGSPHSSRRCSSSLGVPLTEVVEPLDFEDVL LSRPPDAEPGPLRDLVEFPADDLELLLQPRECRTTEPGIPKDEKLDAQVRAAVEMYIEDW VIVHRRYQYLSAAYSPVTTDTQRERQKGLPRQVFEQDASGDERSGPEDSNDSRRGSGSPE DTPRSSGASSIFDLRNLAADSLLPSLLERAAPEDVDRRNETLRRQHRPPALLTLYPAPDE DEAVERCSRPEPPREHFGQRILVKCLSLKFEIEIEPIFGILALYDVREKKKISENFYFDL NSDSMKGLLRAHGTHPAISTLARSAIFSVTYPSPDIFLVIKLEKVLQQGDISECCEPYMV LKEVDTAKNKEKLEKLRLAAEQFCTRLGRYRMPFAWTAVHLANIVSSAGQLDRDSDSEGE RRPAWTDRRRRGPQDRASSGDDACSFSGFRPATLTVTNFFKQEAERLSDEDLFKFLADMR RPSSLLRRLRPVTAQLKIDISPAPENPHFCLSPELLHIKPYPDPRGRPTKEILEFPAREV YAPHTSYRNLLYVYPHSLNFSSRQGSVRNLAVRVQYMTGEDPSQALPVIFGKSSCSEFTR EAFTPVVYHNKSPEFYEEFKLHLPACVTENHHLLFTFYHVSCQPRPGTALETPVGFTWIP LLQHGRLRTGPFCLPVSVDQPPPSYSVLTPDVALPGMRWVDGHKGVFSVELTAVSSVHPQ DPYLDKFFTLVHVLEEGAFPFRLKDTVLSEGNVEQELRASLAALRLASPEPLVAFSHHVL DKLVRLVIRPPIISGQIVNLGRGAFEAMAHVVSLVHRSLEAAQDARGHCPQLAAYVHYAF RLPGTEPSLPDGAPPVTVQAATLARGSGRPASLYLARSKSISSSNPDLAVAPGSVDDEVS RILASKLLHEELALQWVVSSSAVREAILQHAWFFFQLMVKSMALHLLLGQRLDTPRKLRF PGRFLDDITALVGSVGLEVITRVHKDVELAEHLNASLAFFLSDLLSLVDRGFVFSLVRAH YKQVATRLQSSPNPAALLTLRMEFTRILCSHEHYVTLNLPCCPLSPPASPSPSVSSTTSQ SSTFSSQAPDPKVTSMFELSGPFRQQHFLAGLLLTELALALEPEAEGAFLLHKKAISAVH SLLCGHDTDPRYAEATVKARVAELYLPLLSIARDTLPRLHDFAEGPGQRSRLASMLDSDT EGEGDIAGTINPSVAMAIAGGPLAPGSRASISQGPPTASRAGCALSAESSRTLLACVLWV LKNTEPALLQRWATDLTLPQLGRLLDLLYLCLAAFEYKGKKAFERINSLTFKKSLDMKAR LEEAILGTIGARQEMVRRSRERSPFGNPENVRWRKSVTHWKQTSDRVDKTKDEMEHEALV EGNLATEASLVVLDTLEIIVQTVMLSEARESVLGAVLKVVLYSLGSAQSALFLQHGLATQ RALVSKFPELLFEEDTELCADLCLRLLRHCGSRISTIRTHASASLYLLMRQNFEIGHNFA RVKMQVTMSLSSLVGTTQNFSEEHLRRSLKTILTYAEEDMGLRDSTFAEQVQDLMFNLHM ILTDTVKMKEHQEDPEMLIDLMYRIARGYQGSPDLRLTWLQNMAGKHAELGNHAEAAQCM VHAAALVAEYLALLEDHRHLPVGCVSFQNISSNVLEESAISDDILSPDEEGFCSGKHFTE LGLVGLLEQAAGYFTMGGLYEAVNEVYKNLIPILEAHRDYKKLAAVHGKLQEAFTKIMHQ SSGWERVFGTYFRVGFYGAHFGDLDEQEFVYKEPSITKLAEISHRLEEFYTERFGDDVVE IIKDSNPVDKSKLDSQKAYIQITYVEPYFDTYELKDRVTYFDRNYGLRTFLFCTPFTPDG RAHGELPEQHKRKTLLSTDHAFPYIKTRIRVCHREETVLTPVEVAIEDMQKKTRELAFAT EQDPPDAKMLQMVLQGSVGPTVNQGPLEVAQVFLAEIPEDPKLFRHHNKLRLCFKDFCKK CEDALRKNKALIGPDQKEYHRELERNYCRLREALQPLLTQRLPQLMAPTPPGLRNSLNRA SFRKADLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
