MEGF11 (H. sapiens )
In pANT7_cGST (GST-tagged
In vitro expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format: 
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878375
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3135nts
Open reading frame: 1 to 3135

ATGGTGCTCTCCCTGACGGGGCTCATTGCCTTCTCCTTCCTGCAAGCCACCCTTGCCCTG
AACCCCGAGGACCCCAACGTGTGCAGCCACTGGGAGAGCTATGCTGTGACTGTCCAGGAA
TCGTATGCACACCCCTTCGATCAGATCTATTACACACGATGCACAGACATCCTCAACTGG
TTCAAGTGCACCAGGCACCGGATCAGTTATAAGACGGCGTATCGGAGAGGCCTCCGGACC
ATGTACCGGCGGAGGTCCCAGTGCTGCCCTGGCTACTATGAGAGCGGAGACTTCTGCATA
CCCCTGTGTACGGAGGAGTGTGTGCACGGCCGCTGCGTTTCCCCGGACACCTGCCACTGC
GAGCCTGGCTGGGGAGGGCCCGACTGCTCCAGCGGCTGCGACAGCGACCACTGGGGGCCC
CACTGCAGCAACCGGTGCCAGTGCCAGAACGGCGCCCTGTGTAACCCCATCACAGGCGCC
TGCGTGTGCGCCGCCGGCTTCCGTGGATGGCGCTGCGAGGAGCTCTGCGCACCTGGCACC
CACGGCAAGGGATGCCAGCTGCCGTGCCAGTGCCGACACGGTGCCAGCTGCGACCCCCGC
GCCGGCGAGTGCCTCTGCGCACCTGGCTACACCGGCGTCTACTGCGAGGAGCTGTGCCCT
CCTGGGAGCCATGGAGCTCACTGTGAGCTGCGCTGCCCCTGTCAGAATGGGGGCACCTGC
CACCACATCACTGGCGAGTGTGCCTGCCCCCCAGGCTGGACGGGAGCAGTGTGTGCCCAG
CCCTGCCCACCAGGGACATTTGGCCAGAACTGCAGCCAGGATTGTCCTTGCCACCATGGA
GGGCAGTGTGACCACGTGACTGGACAGTGCCACTGTACAGCTGGATACATGGGGGACAGG
TGCCAAGAGGAGTGCCCCTTCGGGTCCTTCGGCTTCCAGTGCTCACAGCACTGTGACTGC
CACAATGGGGGGCAGTGTTCACCCACCACGGGTGCCTGCGAGTGTGAGCCTGGCTACAAG
GGCCCACGCTGCCAGGAGCGACTGTGCCCGGAGGGCCTGCATGGCCCAGGCTGCACCCTG
CCCTGCCCCTGTGACGCTGACAACACCATCAGCTGCCACCCAGTAACTGGAGCTTGTACC
TGCCAGCCAGGCTGGTCTGGTCACCACTGCAATGAATCCTGCCCTGTTGGCTACTATGGC
GATGGCTGCCAGCTGCCTTGCACCTGTCAGAATGGCGCCGACTGCCACAGCATCACTGGG
GGCTGCACTTGTGCTCCGGGCTTCATGGGAGAGGTCTGTGCCGTTTCCTGTGCAGCAGGG
ACCTATGGCCCCAACTGCTCGTCCATCTGTAGCTGTAACAATGGTGGCACCTGCTCCCCA
GTAGATGGCTCCTGTACCTGCAAGGAAGGGTGGCAGGGCCTGGACTGCACCCTGCCATGT
CCCAGTGGGACGTGGGGCCTGAACTGCAACGAGAGCTGCACCTGTGCCAATGGGGCAGCC
TGCAGCCCCATAGACGGCTCCTGCTCCTGCACTCCTGGCTGGCTGGGAGACACCTGTGAG
CTGCCTTGCCCGGATGGCACATTTGGGCTGAACTGCAGTGAACACTGTGACTGCAGCCAT
GCTGATGGATGTGACCCCGTCACAGGCCACTGCTGCTGCCTGGCCGGATGGACAGGCATC
CGCTGTGACAGCACGTGTCCACCTGGCCGCTGGGGCCCCAACTGCTCTGTCTCCTGCAGC
TGTGAGAATGGAGGCTCCTGCTCCCCAGAGGATGGGAGCTGCGAGTGTGCCCCTGGCTTC
CGAGGACCCTTATGCCAGAGAATCTGCCCCCCTGGGTTCTATGGCCACGGCTGCGCCCAG
CCATGCCCCCTCTGCGTGCACAGCAGCAGGCCCTGCCACCACATCAGCGGCATCTGTGAG
TGCCTCCCAGGATTCTCTGGAGCTCTCTGCAACCAAGTGTGTGCTGGAGGATACTTTGGG
CAGGACTGTGCCCAGCTCTGCTCCTGTGCCAACAACGGGACCTGCAGCCCTATCGATGGC
TCCTGCCAGTGCTTTCCTGGATGGATTGGCAAGGACTGCTCACAGGCTTGCCCACCCGGG
TTCTGGGGCCCCGCCTGCTTCCACGCATGCAGCTGCCACAACGGGGCGAGCTGCAGCGCC
GAGGACGGGGCCTGCCACTGCACCCCTGGCTGGACTGGACTCTTCTGCACACAGCGCTGC
CCAGCAGCATTTTTTGGGAAGGACTGTGGGCGCGTATGCCAGTGTCAGAATGGCGCCAGC
TGTGACCACATCAGTGGCAAGTGCACCTGCCGCACAGGCTTCACCGGGCAACACTGTGAG
CAGAGATGTGCCCCAGGAACCTTTGGCTATGGGTGTCAGCAGCTATGTGAGTGCATGAAC
AACTCCACCTGTGACCATGTCACCGGCACCTGTTACTGCAGCCCTGGCTTCAAAGGAATC
AGGTGTGACCAAGCTGCCCTCATGATGGAGGAGCTGAATCCCTACACCAAGATCAGCCCA
GCACTGGGTGCAGAGCGGCACTCGGTGGGTGCTGTCACAGGCATCATGCTCCTGTTATTC
CTCATTGTGGTGCTGCTGGGCCTATTTGCCTGGCATCGGCGGCGGCAGAAAGAGAAGGGC
CGAGACCTGGCTCCCCGTGTCTCCTACACACCTGCCATGAGGATGACCAGCACCGACTAC
TCCCTCTCAGGTGCTTGTGGAATGGATAGACGTCAGAACACATACATTATGGACAAAGGC
TTCAAAGATTACATGAAAGAATCCGTGTGCAGTTCTAGTACTTGTTCCTTGAATAGCAGT
GAAAACCCTTACGCCACAATTAAGGACCCACCCATCCTCACCTGCAAGCTTCCAGAAAGC
AGCTATGTAGAAATGAAGTCGCCTGTGCACATGGGGTCTCCGTACACAGATGTGCCATCC
TTGTCGACATCTAATAAAAATATATATGAAGTTGAGCCCACAGTCAGTGTGGTCCAAGAA
GGTTGCGGTCATAACTCCAGCTATATCCAGAATGCATACGACCTACCTAGGAACAGCCAT
ATTCCTGGTCATTATGACCTCCTCCCAGTAAGACAGAGCCCTGCCAATGGGCCGTCCCAG
GACAAGCAATCTTTG

Protein sequence predicted based on nucleotide sequence

MVLSLTGLIAFSFLQATLALNPEDPNVCSHWESYAVTVQESYAHPFDQIYYTRCTDILNW
FKCTRHRISYKTAYRRGLRTMYRRRSQCCPGYYESGDFCIPLCTEECVHGRCVSPDTCHC
EPGWGGPDCSSGCDSDHWGPHCSNRCQCQNGALCNPITGACVCAAGFRGWRCEELCAPGT
HGKGCQLPCQCRHGASCDPRAGECLCAPGYTGVYCEELCPPGSHGAHCELRCPCQNGGTC
HHITGECACPPGWTGAVCAQPCPPGTFGQNCSQDCPCHHGGQCDHVTGQCHCTAGYMGDR
CQEECPFGSFGFQCSQHCDCHNGGQCSPTTGACECEPGYKGPRCQERLCPEGLHGPGCTL
PCPCDADNTISCHPVTGACTCQPGWSGHHCNESCPVGYYGDGCQLPCTCQNGADCHSITG
GCTCAPGFMGEVCAVSCAAGTYGPNCSSICSCNNGGTCSPVDGSCTCKEGWQGLDCTLPC
PSGTWGLNCNESCTCANGAACSPIDGSCSCTPGWLGDTCELPCPDGTFGLNCSEHCDCSH
ADGCDPVTGHCCCLAGWTGIRCDSTCPPGRWGPNCSVSCSCENGGSCSPEDGSCECAPGF
RGPLCQRICPPGFYGHGCAQPCPLCVHSSRPCHHISGICECLPGFSGALCNQVCAGGYFG
QDCAQLCSCANNGTCSPIDGSCQCFPGWIGKDCSQACPPGFWGPACFHACSCHNGASCSA
EDGACHCTPGWTGLFCTQRCPAAFFGKDCGRVCQCQNGASCDHISGKCTCRTGFTGQHCE
QRCAPGTFGYGCQQLCECMNNSTCDHVTGTCYCSPGFKGIRCDQAALMMEELNPYTKISP
ALGAERHSVGAVTGIMLLLFLIVVLLGLFAWHRRRQKEKGRDLAPRVSYTPAMRMTSTDY
SLSGACGMDRRQNTYIMDKGFKDYMKESVCSSSTCSLNSSENPYATIKDPPILTCKLPES
SYVEMKSPVHMGSPYTDVPSLSTSNKNIYEVEPTVSVVQEGCGHNSSYIQNAYDLPRNSH
IPGHYDLLPVRQSPANGPSQDKQSL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable