Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878778
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 9042nts
Open reading frame: 1 to 9042
ATGTCAAACATTACGATTGACCCAGATGTCAAACCTGGTGAATATGTCATCAAGAGCCTC
TTTGCAGAATTTGCTGTTCAAGCTGAAAAGAAAATTGAAGTTGTAATGGCCGAACCCTTG
GAGAAGCTATTGTCCAGATCTCTTCAGAGGGGTGAAGATCTTCAGTTTGATCAGTTGATA
AGCTCTATGAGCTCAGTAGCAGAGCACTGTCTCCCTTCCTTACTTCGCACCTTGTTTGAC
TGGTACAGACGCCAAAATGGAACGGAAGATGAATCTTATGAATATAGGCCTCGGTCTAGC
ACAAAGTCTAAGGGGGATGAACAGCAACGTGAAAGAGATTATCTTCTTGAAAGGAGGGAC
TTAGCAGTAGACTTCATTTTTTGTTTAGTTTTAGTTGAAGTTCTAAAGCAGATTCCTGTT
CATCCTGTACCCGATCCCTTAGTTCATGAAGTTCTAAACTTAGCTTTTAAGCACTTTAAA
CATAAGGAAGGATATTCAGGAACCAACACTGGGAATGTGCATATTATTGCTGATTTATAT
GCAGAGGTGATAGGGGTTCTTGCCCAATCAAAGTTTCAGGCTGTAAGGAAGAAGTTTGTG
ACAGAATTAAAAGAACTGCGACAAAAGGAACAAAGCCCACATGTGGTACAAAGTGTCATC
AGCTTAATAATGGGAATGAAATTTTTTCGAGTAAAAATGTATCCTGTAGAAGATTTTGAA
GCATCATTTCAATTTATGCAGGAATGTGCTCAGTATTTCTTAGAAGTGAAAGATAAAGAT
ATAAAACATGCACTTGCTGGTTTATTTGTGGAGATTCTTATCCCTGTAGCTGCTGCTGTT
AAAAATGAAGTGAATGTTCCCTGTTTGAAAAATTTTGTGGAGATGCTTTATCAGACTACT
TTTGAACTGAGCTCGAGAAAGAAGCATTCATTGGCTTTATATCCACTAATTACCTGCCTT
TTATGTGTCAGTCAGAAACAATTTTTTTTAAATAACTGGCATATTTTCCTACAGAACTGT
TTGTCACATTTAAAGAATAAAGATCCGAAAATGTCTCGAGTTGCACTGGAATCTTTGTAT
AGATTATTGTGGGTTTATGTAATTAGAATAAAATGTGAAAGCAACACTGTAACTCAAAGT
CGTCTTATGAGCATAGTGTCAGCACTTTTTCCAAAAGGCTCACGAAGTGTGGTTCCTCGT
GACACACCTCTCAATATATTTGTGAAGATTATTCAGTTCATTGCTCAGGAACGCTTGGAT
TTTGCAATGAAAGAAATAATATTTGATCTTCTCAGTGTTGGAAAATCTACTAAAACTTTC
ACCATTAATCCAGAGAGAATGAACATAGGTCTCAGAGTCTTCCTTGTAATAGCAGACAGT
TTGCAGCAGAAAGATGGTGAACCACCCATGCCAACAACAGGAGTTATTCTTCCCTCAGGA
AATACTCTTCGTGTAAAGAAAATATTTCTCAATAAAACCTTGACAGATGAAGAAGCAAAA
GTCATAGGAATGTCAGTTTACTATCCTCAAGTAAGAAAAGCATTAGATAGCATCCTCAGA
CATTTGGACAAAGAAGTTGGGAGACCAATGTGTATGACCAGTGTGCAGATGTCTAATAAG
GAGCCTGAAGACATGATTACGGGGGAAAGAAAACCCAAGATTGATTTGTTTAGAACTTGT
ATTGCTGCGATTCCAAGGTTGATTCCTGACGGTATGAGCAGAACTGACCTGATTGAATTG
TTAGCAAGGCTCACAATTCATATGGATGAAGAACTGCGTGCTCTGGCTTTCAATACTCTG
CAGGCACTAATGCTTGATTTTCCAGATTGGCGGGAGGATGTTCTTTCAGGATTTGTTTAT
TTTATTGTTCGTGAAGTGACTGATGTCCATCCCACACTTCTTGATAATGCCGTAAAGATG
TTGGTACAATTAATAAATCAGTGGAAACAAGCAGCCCAAATGCATAATAAAAACCAGGAC
ACTCAGCATGGTGTAGCTAATGGAGCTTCTCATCCCCCTCCTCTGGAAAGGAGCCCATAT
TCCAATGTATTCCATGTGGTTGAAGGCTTTGCGCTTGTCATTCTCTGTAGCAGTCGACCT
GCCACTAGGAGACTAGCCGTCAGTGTCCTTAGAGAAATACGGGCTTTATTTGCACTTCTG
GAAATACCTAAGGGTGATGATGAATTAGCCATAGATGTGATGGACAGGCTAAGCCCATCC
ATTCTTGAGAGCTTCATACATCTCACTGGGGCTGATCAGACTACTTTGCTCTATTGCCCT
AGCTCGATAGATTTACAAACTTTAGCAGAATGGAACTCTTCTCCTATTAGCCACCAGTTT
GATGTGATTAGTCCATCACATATATGGATATTTGCACATGTGACCCAAGGCCAAGACCCA
TGGATTATAAGTCTCTCCAGTTTTTTAAAGCAAGAAAATCTTCCTAAACACTGCTCTACA
GCTGTGAGCTATGCTTGGATGTTTGCATACACAAGACTTCAGTTGTTGTCCCCTCAGGTC
GATATAAATAGCCCCATCAATGCTAAGAAAGTAAATACCACCACAAGCAGTGACTCATAC
ATTGGCCTGTGGAGAAACTATCTGATCCTTTGCTGCAGTGCAGCAACATCGTCATCTTCC
ACATCTGCAGGTTCTGTGAGATGTTCTCCTCCTGAGACGCTGGCGTCTACCCCAGATAGC
GGCTATAGCATTGATTCTAAAATTATTGGCATCCCATCCCCTTCATCCTTGTTTAAGCAC
ATAGTTCCAATGATGCGTTCTGAGAGCATGGAAATCACAGAATCCCTTGTTCTAGGTCTT
GGCAGGACCAACCCAGGAGCTTTTAGGGAACTAATAGAGGAATTACATCCCATAATTAAA
GAAGCACTCGAAAGAAGGCCAGAGAATATGAAACGGCGCAGGCGTCGAGACATTTTACGA
GTACAACTGGTACGAATATTTGAACTGCTGGCAGATGCTGGTGTCATTAGTCACAGTGCA
AGTGGTGGCCTTGATAATGAAACACATTTTCTCAACAACACTTTATTGGAATATGTAGAT
TTAACTAGACAACTCCTGGAAGCAGAAAATGAAAAAGACTCTGACACACTGAAGGATATA
CGATGCCATTTTAGTGCCTTAGTGGCGAATATTATTCAGAATGTTCCAGTGCACCAGAGA
AGAAGTATTTTTCCTCAACAGAGCCTTCGTCACAGTCTATTTATGCTGTTCAGTCACTGG
GCAGGTCCTTTTAGCATCATGTTTACGCCCTTGGACAGATACAGTGATAGAAATATGCAA
ATTAATAGACATCAATACTGTGCGTTAAAGGCTATGTCTGCTGTACTGTGTTGTGGCCCT
GTTGCAGATAATGTAGGACTTTCATCAGATGGCTATTTGTACAAATGGTTGGATAACATT
TTGGATTCTCTGGACAAAAAGGTTCACCAGCTGGGCTGTGAAGCAGTTACGTTGTTACTG
GAGCTGAACCCTGATCAGAGCAACCTGATGTACTGGGCTGTGGACCGCTGCTACACGGGC
TCCGGGAGGGTGGCGGCCGGCTGCTTTAAAGCCATTGCTAATGTTTTCCAGAACAGGGAT
TATCAATGTGACACAGTGATGCTTCTAAATCTGATACTGTTTAAAGCAGCTGATTCTTCT
AGAAGTATCTATGAAGTTGCTATGCAACTTTTACAGATTCTGGAACCGAAGATGTTTCGC
TATGCTCACAAATTGGAGGTTCAGAGAACAGATGGAGTACTCAGCCAGCTGTCTCCTCTA
CCACATCTCTATTCTGTTTCATATTATCAGTTGTCCGAGGAACTAGCAAGGGCGTATCCT
GAGCTAACTCTCGCCATATTCTCAGAGATAAGCCAGAGAATCCAGACAGCTCACCCTGCT
GGGCGGCAGGTGATGCTGCACTACCTGCTACCATGGATGAACAACATCGAGCTGGTGGAC
TTAAAACCTCTCCCCACAGCAAGGCGACATGATGAAGATGAAGATGATTCCTTAAAAGAC
CGAGAACTTATGGTGACTAGTAGGCGCTGGTTACGGGGAGAAGGATGGGGATCTCCACAA
GCCACTGCAATGGTTTTGAACAATCTGATGTATATGACAGCAAAGTATGGCGATGAACTG
GCCTGGTCGGAGGTGGAGAATGTGTGGACCACACTTGCAGATGGCTGGCCCAAAAACCTG
AAAATAATTTTGCACTTTTTGATCAGCATTTGTGGGGTGAATAGCGAACCAAGCCTCTTG
CCTTACGTGAAGAAGGTCATTGTATATTTAGGTAGAGATAAAACAATGCAGTTGCTAGAA
GAGCTGGTGAGTGAGCTTCAGCTGACCGATCCTGTCAGTTCAGGGGTCACTCACATGGAT
AATCCCCCGTATTATCGCATCACTTCCAGCTATAAAATCCCTTCTGTCACCTCAGGAACT
ACTTCCAGTAGCAATACAATGGTAGCTCCCACAGATGGCAATCCTGATAATAAGCCCATT
AAAGAGAATATTGAAGAGAGCTATGTGCACCTGGACATTTACAGTGGACTAAACAGTCAT
TTGAATCGGCAACATCACAGACTAGAATCCCGATACAGTAGCAGCTCTGGAGGATCTTAT
GAAGAAGAAAAAAGTGATTCAATGCCACTTTATTCTAATTGGCGACTGAAAGTGATGGAG
CATAACCAAGGAGAGCCACTGCCCTTCCCACCAGCTGGAGGCTGCTGGTCACCACTGGTG
GATTACGTGCCTGAAACGTCATCACCTGGATTACCTCTTCACAGGTGTAACATAGCAGTG
ATCCTTTTGACTGATCTCATCATTGATCATAGTGTGAAGGTGGAATGGGGAAGCTACCTC
CATCTTCTTCTTCATGCAATTTTTATAGGGTTTGACCACTGCCACCCTGAGGTGTATGAA
CATTGTAAACGCCTGCTTCTGCACTTATTAATAGTAATGGGACCCAATAGTAACATCCGA
ACTGTTGCTTCTGTCCTTCTCAGGAACAAGGAGTTTAATGAGCCCAGGGTGCTTACAGTC
AAACAAGTTGCACACTTAGATTATAATTTCACAGCAGGCATTAACGATTTTATACCTGAT
TACCAGCCCTCCCCTATGACTGACTCAGGGCTTAGCTCAAGTTCTACCTCTTCTAGTATC
AGCTTAGGAAATAACAGTGCTGCCATTTCACATCTGCACACCACTATCCTCAATGAGGTT
GACATCTCAGTGGAGCAGGATGGAAAAGTCAAAACCCTCATGGAATTCATTACCTCAAGA
AAAAGAGGGCCCCTTTGGAACCATGAGGATGTTTCTGCCAAGAATCCTAGCATAAAGAGT
GCTGAACAGTTAACTACATTTTTGAAACATGTGGTTTCTGTTTTTAAGCAGTCAAGCTCA
GAAGGAATTCATCTGGAACATCATCTTAGTGAAGTTGCTCTGCAAACAGCACTTTCCTGT
TCTTCTCGACACTATGCTGGGAGATCCTTTCAGATTTTCAGGGCCCTAAAGCAGCCTCTC
ACTGCAACTACACTTTCTGATGTTCTCTCCAGACTTGTAGAAACTGTAGGGGATCCAGGA
GAAGATGCACAGGGATTTGTGATTGAGCTTCTTCTCACATTGGAATCTGCAATTGATACT
TTGGCTGAAACCATGAAGCATTATGATCTTCTTTCTGCCCTTTCTCAAACCTCATATCAT
GATCCTATAATGGGAAACAAGTATGCAGCTAACAGGAAAAGCACTGGACAACTCAATCTA
AGCACAAGTCCCATTAATAGTAGCAGTTATTTGGGATATAACAGTAATGCAAGAAGTAAC
TCTTTGAGATTAAGTTTGATTGGTGACCGACGAGGTGACCGGCGGCGGAGTAACACACTG
GATATAATGGATGGACGGATAAACCATAGCAGTAGTTTAGCAAGGACTAGAAGCCTTTCC
TCTCTAAGAGAGAAAGGAATGTATGACGTGCAGTCCACTACTGAGCCTACCAACTTGATG
GCCACCATTTTTTGGATAGCAGCATCTTTATTAGAATCAGATTATGAATATGAATACCTC
CTGGCTCTCAGGCTTCTCAACAAACTGCTTATCCATTTGCCTTTGGATAAATCAGAGAGT
CGAGAGAAGATTGAAAATGTACAAAGCAAATTGAAATGGACTAATTTTCCAGGACTTCAG
CAGCTCTTCCTTAAGGGTTTTACCTCAGCATCTACACAAGAAATGACCGTGCACCTCCTC
AGTAAACTCATTTCTGTCTCCAAACATACATTGGTGGATCCTTCCCAATTGTCAGGCTTT
CCTCTTAACATCCTTTGCTTATTGCCTCACTTAATCCAGCATTTTGACAGCCCAACTCAG
TTTTGCAAAGAAACAGCTAGTCGAATAGCAAAGGTTTGTGCAGAAGAAAAATGCCCAACA
CTTGTCAATCTGGCACACATGATGAGTTTGTACAGTACACACACGTATTCCAGAGACTGT
TCTAACTGGATCAATGTCGTGTGCAGATACCTGCATGACTCCTTCTCAGATACAACATTT
AATCTTGTGACTTATCTTGCAGAGCTGTTAGAGAAAGGATTGTCCAGTATGCAGCAATCA
TTACTACAGATTATTTATAGTCTATTGAGTCATATTGACCTGTCTGCAGCCCCAGCCAAG
CAGTTTAATCTGGAGATCATAAAGATTATTGGCAAATATGTACAGAGTCCTTACTGGAAG
GAAGCCCTTAACATATTAAAGCTGGTGGTGTCACGCTCTGCGAGTCTTGTCGTACCCAGT
GATATCCCCAAGACCTATGGAGGAGATACAGGTTCTCCTGAAATATCCTTCACTAAAATT
TTTAATAATGTTTCTAAGGAGTTGCCTGGGAAGACCTTAGATTTTCATTTTGATATATCT
GAGACACCAATTATTGGAAACAAATATGGTGATCAGCACAGTGCGGCTGGAAGAAATGGG
AAACCAAAAGTTATTGCTGTCACTAGAAGTACTTCCTCAACTTCTTCTGGTTCTAATTCT
AATGCCTTGGTTCCTGTTAGTTGGAAAAGGCCACAGTTATCACAGCGAAGAACAAGAGAA
AAGCTAATGAATGTGCTTTCTCTCTGTGGTCCAGAATCTGGCCTCCCAAAGAACCCATCA
GTTGTATTTTCTTCTAATGAGGATTTGGAAGTCGGTGACCAACAGACTAGCCTAATTTCT
ACAACAGAAGACATAAATCAAGAGGAAGAAGTAGCTGTGGAAGATAATAGCAGTGAACAA
CAGTTTGGTGTTTTTAAGGATTTTGACTTTTTAGATGTTGAATTGGAAGATGCAGAGGGT
GAAAGTATGGACAATTTCAACTGGGGAGTTCGCAGGCGCTCACTGGACAGTATTGACAAA
GGGGACACTCCATCCCTCCAGGAGTACCAGTGCTCTAGTAGCACCCCCAGCCTGAACCTC
ACCAATCAGGAGGATACAGATGAGTCCTCGGAAGAAGAAGCGGCACTTACAGCAAGCCAG
ATACTCTCACGCACACAGATGTTAAACAGTGATTCTGCCACTGATGAAACAATACCAGAC
CATCCTGACTTACTTCTCCAGTCTGAAGATTCCACTGGCAGCATCACAACAGAGGAAGTG
CTTCAAATCAGGGATGAGACCCCAACTTTGGAGGCTTCTCTAGATAATGCTAACAGCCGG
CTGCCTGAGGATACAACTTCAGTATTAAAGGAGGAACATGTTACAACCTTTGAAGATGAA
GGATCCTATATAATTCAAGAACAGCAGGAATCTCTTGTGTGTCAAGGAATTCTTGATTTA
GAAGAAACTGAAATGCCAGAGCCTCTAGCTCCTGAAAGTTACCCCGAGTCAGTCTGTGAA
GAGGATGTTACCTTAGCTCTGAAAGAGCTAGATGAAAGATGTGAAGAAGAAGAAGCGGAT
TTCTCCGGACTGTCTAGTCAAGATGAAGAAGAGCAAGATGGTTTTCCAGAAGTACAGACG
TCGCCTCTGCCGTCACCATTTCTTTCTGCCATCATAGCCGCCTTTCAGCCCGTGGCATAT
GATGATGAAGAGGAAGCCTGGCGCTGCCACGTCAATCAGATGCTGTCTGACACCGACGGG
TCCTCTGCAGTGTTTACTTTTCATGTGTTTTCTAGGCTGTTTCAGACAATTCAAAGAAAG
TTTGGAGAAATAACTAATGAGGCAGTCAGCTTTCTTGGTGATAGTCTGCAACGCATTGGT
ACCAAATTTAAAAGTTCCTTGGAAGTGATGATGCTGTGTTCAGAATGCCCAACAGTCTTT
GTGGATGCTGAAACACTGATGTCATGTGGTTTGCTGGAAACACTCAAGTTTGGTGTTTTG
GAGTTGCAAGAACACCTGGATACATACAATGTGAAAAGAGAAGCCGCTGAGCAGTGGCTA
GATGATTGTAAGAGGACATTTGGTGCCAAAGAAGACATGTATAGGATAAACACAGATGCA
CAACAAATGGAAATACTAGCAGAATTGGAGCTCTGCCGAAGATTATACAAATTGCATTTT
CAATTGCTGCTTCTGTTCCAGGCCTACTGTAAACTTATCAACCAAGTAAATACGATAAAA
AATGAAGCAGAGGTCATCAACATGTCAGAGGAACTTGCCCAACTGGAAAGTATCCTCAAA
GAAGCTGAGTCCGCTTCCGAAAACGAAGAAATTGACATTTCCAAAGCTGCACAAACTACT
ATAGAAACTGCCATTCATTCTTTAATTGAAACTTTGAAAAATAAAGAATTTATATCAGCT
GTAGCACAAGTCAAAGCTTTCAGATCTCTCTGGCCCAGTGATATCTTTGGCAGTTGTGAA
GATGACCCTGTACAGACACTGTTACATATATATTTCCATCATCAGACGCTGGGCCAGACA
GGAAGCTTTGCAGTTATAGGCTCTAACCTGGACATGTCAGAAGCCAACTACAAACTGATG
GAACTTAATCTGGAAATAAGAGAGTCTCTACGCATGGTGCAATCATACCAACTTCTAGCA
CAGGCCAAACCAATGGGAAATATGGTGAGCACTGGATTCTTG
Protein sequence predicted based on nucleotide sequence
MSNITIDPDVKPGEYVIKSLFAEFAVQAEKKIEVVMAEPLEKLLSRSLQRGEDLQFDQLI SSMSSVAEHCLPSLLRTLFDWYRRQNGTEDESYEYRPRSSTKSKGDEQQRERDYLLERRD LAVDFIFCLVLVEVLKQIPVHPVPDPLVHEVLNLAFKHFKHKEGYSGTNTGNVHIIADLY AEVIGVLAQSKFQAVRKKFVTELKELRQKEQSPHVVQSVISLIMGMKFFRVKMYPVEDFE ASFQFMQECAQYFLEVKDKDIKHALAGLFVEILIPVAAAVKNEVNVPCLKNFVEMLYQTT FELSSRKKHSLALYPLITCLLCVSQKQFFLNNWHIFLQNCLSHLKNKDPKMSRVALESLY RLLWVYVIRIKCESNTVTQSRLMSIVSALFPKGSRSVVPRDTPLNIFVKIIQFIAQERLD FAMKEIIFDLLSVGKSTKTFTINPERMNIGLRVFLVIADSLQQKDGEPPMPTTGVILPSG NTLRVKKIFLNKTLTDEEAKVIGMSVYYPQVRKALDSILRHLDKEVGRPMCMTSVQMSNK EPEDMITGERKPKIDLFRTCIAAIPRLIPDGMSRTDLIELLARLTIHMDEELRALAFNTL QALMLDFPDWREDVLSGFVYFIVREVTDVHPTLLDNAVKMLVQLINQWKQAAQMHNKNQD TQHGVANGASHPPPLERSPYSNVFHVVEGFALVILCSSRPATRRLAVSVLREIRALFALL EIPKGDDELAIDVMDRLSPSILESFIHLTGADQTTLLYCPSSIDLQTLAEWNSSPISHQF DVISPSHIWIFAHVTQGQDPWIISLSSFLKQENLPKHCSTAVSYAWMFAYTRLQLLSPQV DINSPINAKKVNTTTSSDSYIGLWRNYLILCCSAATSSSSTSAGSVRCSPPETLASTPDS GYSIDSKIIGIPSPSSLFKHIVPMMRSESMEITESLVLGLGRTNPGAFRELIEELHPIIK EALERRPENMKRRRRRDILRVQLVRIFELLADAGVISHSASGGLDNETHFLNNTLLEYVD LTRQLLEAENEKDSDTLKDIRCHFSALVANIIQNVPVHQRRSIFPQQSLRHSLFMLFSHW AGPFSIMFTPLDRYSDRNMQINRHQYCALKAMSAVLCCGPVADNVGLSSDGYLYKWLDNI LDSLDKKVHQLGCEAVTLLLELNPDQSNLMYWAVDRCYTGSGRVAAGCFKAIANVFQNRD YQCDTVMLLNLILFKAADSSRSIYEVAMQLLQILEPKMFRYAHKLEVQRTDGVLSQLSPL PHLYSVSYYQLSEELARAYPELTLAIFSEISQRIQTAHPAGRQVMLHYLLPWMNNIELVD LKPLPTARRHDEDEDDSLKDRELMVTSRRWLRGEGWGSPQATAMVLNNLMYMTAKYGDEL AWSEVENVWTTLADGWPKNLKIILHFLISICGVNSEPSLLPYVKKVIVYLGRDKTMQLLE ELVSELQLTDPVSSGVTHMDNPPYYRITSSYKIPSVTSGTTSSSNTMVAPTDGNPDNKPI KENIEESYVHLDIYSGLNSHLNRQHHRLESRYSSSSGGSYEEEKSDSMPLYSNWRLKVME HNQGEPLPFPPAGGCWSPLVDYVPETSSPGLPLHRCNIAVILLTDLIIDHSVKVEWGSYL HLLLHAIFIGFDHCHPEVYEHCKRLLLHLLIVMGPNSNIRTVASVLLRNKEFNEPRVLTV KQVAHLDYNFTAGINDFIPDYQPSPMTDSGLSSSSTSSSISLGNNSAAISHLHTTILNEV DISVEQDGKVKTLMEFITSRKRGPLWNHEDVSAKNPSIKSAEQLTTFLKHVVSVFKQSSS EGIHLEHHLSEVALQTALSCSSRHYAGRSFQIFRALKQPLTATTLSDVLSRLVETVGDPG EDAQGFVIELLLTLESAIDTLAETMKHYDLLSALSQTSYHDPIMGNKYAANRKSTGQLNL STSPINSSSYLGYNSNARSNSLRLSLIGDRRGDRRRSNTLDIMDGRINHSSSLARTRSLS SLREKGMYDVQSTTEPTNLMATIFWIAASLLESDYEYEYLLALRLLNKLLIHLPLDKSES REKIENVQSKLKWTNFPGLQQLFLKGFTSASTQEMTVHLLSKLISVSKHTLVDPSQLSGF PLNILCLLPHLIQHFDSPTQFCKETASRIAKVCAEEKCPTLVNLAHMMSLYSTHTYSRDC SNWINVVCRYLHDSFSDTTFNLVTYLAELLEKGLSSMQQSLLQIIYSLLSHIDLSAAPAK QFNLEIIKIIGKYVQSPYWKEALNILKLVVSRSASLVVPSDIPKTYGGDTGSPEISFTKI FNNVSKELPGKTLDFHFDISETPIIGNKYGDQHSAAGRNGKPKVIAVTRSTSSTSSGSNS NALVPVSWKRPQLSQRRTREKLMNVLSLCGPESGLPKNPSVVFSSNEDLEVGDQQTSLIS TTEDINQEEEVAVEDNSSEQQFGVFKDFDFLDVELEDAEGESMDNFNWGVRRRSLDSIDK GDTPSLQEYQCSSSTPSLNLTNQEDTDESSEEEAALTASQILSRTQMLNSDSATDETIPD HPDLLLQSEDSTGSITTEEVLQIRDETPTLEASLDNANSRLPEDTTSVLKEEHVTTFEDE GSYIIQEQQESLVCQGILDLEETEMPEPLAPESYPESVCEEDVTLALKELDERCEEEEAD FSGLSSQDEEEQDGFPEVQTSPLPSPFLSAIIAAFQPVAYDDEEEAWRCHVNQMLSDTDG SSAVFTFHVFSRLFQTIQRKFGEITNEAVSFLGDSLQRIGTKFKSSLEVMMLCSECPTVF VDAETLMSCGLLETLKFGVLELQEHLDTYNVKREAAEQWLDDCKRTFGAKEDMYRINTDA QQMEILAELELCRRLYKLHFQLLLLFQAYCKLINQVNTIKNEAEVINMSEELAQLESILK EAESASENEEIDISKAAQTTIETAIHSLIETLKNKEFISAVAQVKAFRSLWPSDIFGSCE DDPVQTLLHIYFHHQTLGQTGSFAVIGSNLDMSEANYKLMELNLEIRESLRMVQSYQLLA QAKPMGNMVSTGFL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
