Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878876
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 8994nts
Open reading frame: 1 to 8994
ATGGCAGATCCAGGAATGATGAGTCTTTTTGGCGAGGATGGGAATATTTTCAGTGAAGGT
CTTGAAGGCCTCGGAGAATGTGGTTACCCGGAAAATCCAGTAAATCCTATGGGTCAGCAA
ATGCCAATAGACCAAGGCTTTGCCTCTTTACAGCCATCCCTTCATCATCCTTCAACTAAT
CAAAATCAAACAAAGCTGACACATTTTGATCACTATAATCAGTATGAACAACAAAAGATG
CATCTGATGGATCAGCCGAACAGAATGATGAGCAACACCCCTGGGAACGGACTCGCGTCT
CCGCACTCGCAGTATCACACCCCTCCCGTTCCTCAGGTGCCCCATGGTGGCAGTGGTGGC
GGTCAGATGGGTGTCTACCCTGGCATGCAGAATGAGAGGCATGGGCAATCCTTTGTGGAC
AGCAGCTCCATGTGGGGCCCCAGGGCTGTTCAGGTACCAGACCAGATACGAGCCCCCTAC
CAGCAGCAGCAGCCACAGCCGCAGCCACCGCAGCCGGCTCCGTCGGGGCCCCCTGCACAG
GGCCACCCTCAGCACATGCAGCAGATGGGCAGCTATATGGCACGTGGGGATTTTTCCATG
CAGCAGCATGGTCAGCCACAGCAGAGGATGAGCCAGTTTTCCCAAGGCCAAGAGGGCCTC
AATCAGGGAAATCCTTTTATTGCCACCTCAGGACCTGGCCACTTGTCCCACGTGCCCCAG
CAGAGTCCCAGCATGGCACCTTCCTTGCGTCACTCGGTGCAGCAGTTCCATCACCACCCC
TCTACTGCTCTCCATGGAGAATCCGTTGCCCACAGTCCCAGATTCTCCCCGAATCCTCCC
CAACAAGGGGCTGTTAGGCCGCAAACCCTTAACTTTAGTTCTCGGAGCCAGACAGTCCCC
TCTCCTACTATAAACAACTCAGGGCAGTATTCTCGATATCCTTACAGTAACCTAAATCAG
GGATTAGTTAACAATACAGGGATGAATCAAAATTTAGGCCTTACAAATAATACTCCAATG
AATCAGTCCGTACCAAGATACCCCAATGCTGTAGGATTCCCATCAAACAGTGGTCAAGGA
CTAATGCACCAGCAGCCCATCCACCCCAGTGGCTCACTTAACCAAATGAACACACAAACT
ATGCATCCTTCACAGCCTCAGGGAACTTATGCCTCTCCACCTCCCATGTCACCCATGAAA
GCAATGAGTAATCCAGCAGGCACTCCTCCTCCACAAGTCAGGCCGGGAAGTGCTGGGATA
CCAATGGAAGTTGGCAGTTATCCAAATATGCCCCATCCTCAGCCATCTCACCAGCCCCCT
GGTGCCATGGGAATCGGACAGAGGAATATGGGCCCCAGAAACATGCAGCAGTCTCGTCCA
TTTATAGGCATGTCCTCGGCACCAAGGGAATTGACTGGGCACATGAGGCCAAATGGTTGT
CCTGGTGTTGGCCTTGGAGACCCACAAGCAATCCAGGAACGACTGATACCTGGCCAACAA
CATCCTGGTCAACAGCCATCTTTTCAGCAGTTGCCAACCTGTCCTCCACTGCAGCCTCAC
CCGGGCTTGCACCACCAGTCTTCACCTCCACACCCTCATCACCAGCCTTGGGCACAGCTC
CACCCATCACCCCAGAACACCCCGCAGAAAGTGCCTGTGCATCAGCATTCCCCGTCGGAG
CCCTTTCTAGAGAAACCAGTGCCGGATATGACTCAGGTTAGTGGACCGAATGCTCAGCTA
GTGAAGAGTGATGATTACCTGCCATCAATAGAACAGCAGCCACAACAAAAGAAGAAGAAA
AAGAAAAACAACCACATTGTAGCAGAGGATCCCAGTAAAGGTTTTGGTAAAGATGACTTC
CCTGGTGGGGTAGATAACCAAGAACTAAATAGGAACTCACTGGATGGGTCCCAAGAAGAA
AAAAAGAAAAAGAAAAGGTCAAAGGCAAAAAAAGACCCGAAGGAACCGAAAGAACCCAAG
GAGAAAAAAGAGCCCAAGGAACCCAAGACCCCGAAAGCCCCTAAGATTCCCAAAGAGCCA
AAGGAAAAGAAAGCAAAAACTGCCACGCCAAAACCCAAATCCAGCAAAAAGTCAAGTAAT
AAGAAACCTGACTCAGAAGCAAGTGCTTTGAAGAAAAAGGTCAACAAGGGAAAAACAGAA
GGTTCTGAAAATTCAGACTTAGACAAAACACCCCCACCATCTCCTCCTCCTGAAGAAGAT
GAGGACCCAGGTGTTCAGAAGAGACGGTCCAGCAGACAGGTGAAGAGAAAGCGCTACACT
GAAGACCTGGAGTTCAAGATTTCTGATGAGGAGGCAGATGATGCAGATGCTGCTGGGAGG
GATTCCCCCTCCAACACCTCCCAGTCAGAACAGCAGGAATCTGTTGATGCAGAAGGCCCA
GTGGTAGAAAAAATTATGAGCAGTCGTTCAGTAAAAAAGCAGAAGGAATCTGGAGAGGAG
GTAGAAATTGAGGAATTCTATGTGAAATACAAAAACTTCTCTTATCTTCATTGTCAGTGG
GCATCTATAGAAGATCTGGAAAAAGATAAGAGAATTCAGCAAAAAATTAAACGATTTAAG
GCAAAGCAGGGCCAGAACAAGTTCCTTTCAGAGATTGAGGATGAGCTTTTTAATCCAGAT
TATGTGGAGGTTGACCGGATAATGGACTTTGCACGTAGCACAGATGACCGGGGAGAGCCT
GTGACTCACTATCTGGTGAAGTGGTGTTCACTTCCTTATGAAGACAGCACGTGGGAGCGG
AGGCAGGACATAGATCAAGCAAAGATCGAGGAGTTTGAGAAACTAATGTCCAGGGAGCCG
GAAACAGAGCGTGTGGAGCGACCTCCTGCTGATGATTGGAAGAAATCGGAGAGTTCCAGG
GAGTATAAAAACAATAACAAACTCAGGGAATACCAGTTGGAGGGAGTAAACTGGCTACTT
TTCAATTGGTACAACATGCGAAACTGCATTTTAGCAGATGAAATGGGTTTGGGAAAAACT
ATCCAGTCCATTACATTTCTCTATGAGATATATTTGAAAGGAATCCATGGCCCTTTTTTA
GTAATTGCCCCATTGTCCACAATCCCCAACTGGGAAAGGGAATTCCGAACCTGGACAGAG
TTGAACGTGGTTGTGTATCATGGGAGTCAAGCTAGTCGTCGGACCATTCAGTTGTATGAA
ATGTACTTCAAAGATCCCCAGGGTCGAGTGATAAAGGGGTCCTATAAGTTTCATGCCATC
ATCACTACATTTGAGATGATTTTGACTGATTGTCCTGAGCTGCGGAATATTCCATGGCGC
TGTGTAGTCATTGATGAAGCCCACAGGCTGAAGAACAGGAACTGCAAGCTGTTGGAGGGA
CTCAAGATGATGGACTTGGAACACAAAGTGCTGCTGACGGGAACCCCACTCCAGAACACT
GTGGAAGAACTCTTCAGCTTGCTTCATTTCTTGGAACCAAGTCGCTTCCCTTCAGAAACC
ACATTTATGCAAGAATTTGGTGATCTAAAAACAGAAGAGCAGGTGCAAAAACTTCAAGCT
ATTCTAAAGCCAATGATGTTGAGACGTCTCAAAGAGGATGTAGAAAAGAACTTGGCCCCC
AAAGAAGAAACTATTATTGAAGTTGAGCTAACAAACATTCAGAAGAAATATTACCGAGCC
ATCCTTGAGAAGAATTTCACATTTCTTTCCAAAGGCGGTGGTCAAGCTAACGTACCTAAC
CTATTAAACACTATGATGGAATTGCGGAAGTGCTGCAATCATCCGTACCTTATCAATGGT
GCTGAAGAGAAAATTTTGGAAGAGTTTAAAGAAACACACAATGCAGAGTCTCCAGATTTT
CAGCTCCAGGCAATGATCCAGGCTGCTGGCAAGCTAGTGCTGATTGACAAGCTGCTGCCA
AAACTGAAGGCTGGTGGCCACAGGGTGCTTATCTTTTCCCAGATGGTGCGCTGCTTGGAC
ATACTGGAAGACTACCTCATTCAAAGACGGTACCCATATGAAAGGATCGACGGCCGAGTA
AGAGGCAACCTCCGCCAGGCAGCTATCGACAGATTCTCCAAACCTGATTCTGATAGGTTT
GTTTTCCTCCTGTGTACAAGGGCAGGAGGTTTAGGCATTAACCTCACTGCTGCTGATACC
TGCATCATCTTTGATTCAGACTGGAATCCCCAAAATGACCTCCAGGCTCAGGCTAGATGT
CATAGAATAGGACAGAGCAAATCTGTGAAAATCTACAGGCTGATTACAAGAAATTCCTAT
GAAAGGGAAATGTTCGACAAGGCTAGTTTGAAACTGGGCCTGGATAAAGCTGTGCTACAG
TCTATGAGTGGAAGAGAAAATGCTACCAATGGGGTACAACAGCTTTCCAAGAAAGAAATA
GAGGATCTTCTACGAAAAGGGGCCTATGGTGCACTCATGGATGAGGAGGATGAAGGGTCT
AAATTCTGTGAAGAAGATATTGATCAGATCCTCCTACGTCGAACCCACACCATTACCATT
GAGTCAGAAGGGAAAGGTTCCACATTTGCTAAGGCCAGTTTTGTTGCATCTGGAAATAGG
ACAGATATTTCCTTGGATGATCCAAATTTCTGGCAAAAGTGGGCTAAGAAGGCTGAATTG
GATATTGATGCCTTAAATGGGAGGAACAACCTGGTTATTGATACTCCAAGAGTGAGAAAG
CAGACCAGGCTCTACAGTGCAGTGAAGGAAGATGAGCTGATGGAGTTCTCAGACTTGGAA
AGTGATTCTGAAGAAAAGCCCTGTGCAAAGCCACGGCGTCCCCAGGATAAGTCACAGGGC
TATGCAAGGAGTGAATGTTTCAGGGTGGAGAAGAATCTGCTTGTCTATGGTTGGGGACGG
TGGACAGACATTCTTTCCCACGGACGCTATAAACGCCAACTCACTGAGCAAGATGTAGAA
ACCATCTGCAGAACCATCCTGGTGTACTGTCTTAATCATTACAAAGGGGATGAGAATATC
AAAAGCTTCATCTGGGATCTGATCACACCCACAGCGGATGGCCAGACTCGAGCCTTGGTC
AACCATTCCGGTTTGTCAGCTCCTGTGCCAAGGGGAAGGAAGGGAAAGAAGGTGAAAGCC
CAGAGCACACAGCCGGTGGTGCAGGATGCCGACTGGCTGGCCAGCTGCAACCCAGATGCC
CTGTTCCAGGAGGACAGCTACAAGAAACACCTGAAGCATCACTGTAACAAGGTCCTGCTG
CGTGTCCGCATGCTGTACTACCTAAGACAAGAAGTGATAGGAGACCAGGCGGATAAGATC
TTAGAGGGTGCTGACTCAAGTGAAGCCGATGTGTGGATCCCTGAACCTTTCCATGCTGAA
GTTCCTGCAGATTGGTGGGATAAGGAAGCAGACAAATCCCTCTTAATTGGAGTGTTCAAA
CATGGCTATGAGAAGTACAACTCCATGCGAGCTGACCCCGCGCTGTGCTTTCTGGAACGA
GTCGGTATGCCTGATGCCAAGGCCATAGCTGCCGAGCAAAGAGGAACAGACATGCTAGCA
GATGGTGGTGACGGGGGAGAATTTGATAGAGAAGATGAAGACCCAGAATATAAACCAACC
AGAACACCGTTCAAAGATGAAATAGATGAATTTGCAAATTCTCCTTCAGAGGATAAGGAA
GAATCCATGGAAATACATGCCACAGGCAAGCACAGTGAGAGTAATGCTGAGTTAGGCCAA
CTTTACTGGCCTAACACTTCAACCCTGACTACACGTCTGCGCCGGCTCATTACTGCCTAT
CAGCGCAGCTATAAAAGGCAACAGATGAGGCAAGAGGCCCTAATGAAGACTGACCGGCGC
AGACGGCGGCCTCGAGAGGAAGTGAGAGCTCTGGAAGCGGAAAGGGAAGCTATTATATCT
GAGAAGCGGCAAAAGTGGACAAGAAGAGAAGAGGCTGATTTTTACCGTGTGGTATCCACC
TTTGGGGTTATTTTTGACCCTGTGAAACAGCAATTTGACTGGAACCAATTTAGAGCCTTT
GCCAGGCTTGACAAAAAATCTGATGAGAGTTTGGAGAAATACTTCAGTTGTTTTGTGGCC
ATGTGTAGGCGAGTATGTCGAATGCCCGTCAAGCCAGATGATGAACCGCCCGACCTCTCC
TCCATAATTGAGCCGATCACAGAGGAGCGAGCCTCTCGAACTCTGTACCGCATTGAGCTG
CTACGGAAGATCCGCGAGCAGGTTCTCCATCACCCCCAGCTGGGAGAGAGGCTTAAGCTC
TGCCAGCCAAGCTTGGATCTGCCAGAGTGGTGGGAGTGTGGACGGCATGACCGAGACTTG
CTGGTTGGTGCTGCTAAACACGGGGTCAGTCGGACGGATTATCACATCCTCAATGACCCT
GAGTTATCCTTCTTGGATGCACATAAAAACTTTGCTCAAAACAGAGGGGCAGGTAATACA
TCTTCCTTGAACCCACTGGCAGTTGGATTTGTCCAGACTCCTCCAGTCATCTCATCTGCT
CATATTCAAGATGAGAGGGTACTGGAACAAGCCGAAGGCAAAGTGGAGGAGCCTGAAAAC
CCAGCTGCCAAGGAGAAATGTGAGGGCAAAGAAGAGGAAGAAGAAACCGATGGCAGCGGG
AAGGAGAGCAAGCAGGAATGTGAGGCAGAGGCCAGCTCTGTGAAAAATGAACTGAAAGGT
GTTGAGGTCGGCGCAGACACTGGGTCCAAATCTATTTCAGAGAAAGGTTCCGAAGAGGAT
GAAGAGGAAAAGCTGGAGGATGACGATAAGTCGGAAGAGTCTTCCCAGCCCGAAGCAGGA
GCTGTCTCTAGAGGGAAGAATTTTGATGAAGAAAGCAATGCTTCCATGAGCACTGCTAGA
GATGAAACCCGAGATGGATTCTACATGGAGGACGGAGATCCTTCAGTAGCTCAGCTCCTT
CATGAAAGAACATTTGCCTTCTCGTTTTGGCCTAAGGATAGAGTAATGATAAACCGCTTA
GACAACATCTGTGAAGCAGTGTTGAAAGGCAAATGGCCAGTAAATAGGCGCCAGATGTTT
GATTTCCAAGGCCTCATCCCAGGTTACACACCCACCACAGTGGACAGCCCCTTGCAGAAG
AGGAGCTTTGCTGAGCTCTCCATGGTCGGCCAAGCCAGCATTAGTGGGAGTGAGGACATC
ACTACGTCTCCTCAGTTGTCAAAGGAAGATGCCCTCAACCTCTCTGTCCCTCGCCAGCGG
AGGAGGAGGAGGAGAAAAATCGAAATTGAGGCCGAAAGAGCTGCCAAGAGGCGAAATCTC
ATGGAGATGGTTGCCCAGCTTCGAGAGTCTCAGGTGGTCTCAGAAAATGGACAAGAAAAA
GTTGTAGATTTATCAAAGGCCTCAAGAGAGGCAACAAGCTCTACCTCAAATTTTTCATCT
CTTTCTTCAAAGTTTATCTTGCCTAATGTCTCAACACCAGTGTCTGATGCCTTTAAGACT
CAAATGGAACTGCTCCAAGCAGGCCTTTCGCGCACACCCACAAGGCATCTCCTTAATGGC
TCCCTAGTGGATGGAGAGCCTCCCATGAAGAGGAGGCGGGGAAGGAGGAAAAATGTGGAG
GGACTTGATCTGCTTTTCATGAGCCACAAACGGACGTCATTGAGTGCAGAGGATGCTGAG
GTGACCAAAGCTTTTGAAGAAGATATAGAGACCCCACCAACAAGAAACATTCCTTCTCCC
GGACAGCTGGACCCAGACACACGGATCCCTGTTATCAATCTTGAAGATGGGACTAGGCTG
GTGGGGGAAGATGCTCCTAAAAATAAGGATTTAGTTGAATGGCTGAAGCTGCACCCTACT
TACACTGTTGATATGCCAAGTTATGTACCAAAGAATGCAGATGTGCTGTTTTCCTCATTT
CAGAAACCGAAACAGAAACGACATAGATGTCGAAACCCTAATAAATTGGATATAAACACT
TTGACAGGAGAAGAAAGGGTGCCTGTTGTCAATAAACGAAATGGGAAGAAGATGGGTGGA
GCTATGGCGCCTCCAATGAAGGATCTACCCAGGTGGCTGGAAGAAAATCCTGAATTTGCA
GTTGCTCCAGACTGGACTGATATAGTTAAGCAGTCTGGTTTTGTTCCTGAGTCGATGTTT
GACCGCCTTCTCACTGGGCCTGTAGTGCGGGGAGAGGGAGCGAGCAGAAGAGGAAGAAGG
CCCAAAAGTGAGATCGCCAGAGCAGCCGCGGCCGCCGCTGCTGTGGCCTCCACGTCAGGG
ATCAACCCTTTGCTGGTGAACAGCCTGTTTGCTGGAATGGACCTGACGAGCCTTCAGAAT
CTCCAGAATCTCCAGTCGCTCCAGCTGGCAGGCCTCATGGGCTTCCCTCCAGGACTGGCA
ACAGCTGCCACCGCCGGAGGCGATGCGAAGAACCCTGCTGCTGTGCTGCCCCTGATGCTG
CCAGGAATGGCGGGCCTGCCCAACGTGTTTGGCTTGGGCGGGCTGTTGAATAACCCTCTG
TCAGCTGCTACTGGAAACACCACTACTGCTTCTAGTCAAGGAGAACCGGAAGACAGCACT
TCAAAAGGAGAGGAGAAAGGAAATGAGAATGAAGACGAGAACAAAGACTCTGAGAAAAGC
ACAGATGCTGTTTCGGCTGCTGACTCTGCGAATGGATCTGTTGGTGCTGCTACTGCCCCG
GCTGGATTGCCCTCAAACCCGCTAGCCTTCAACCCTTTCCTCCTGTCCACAATGGCCCCG
GGCCTCTTCTACCCATCCATGTTTCTACCTCCAGGACTGGGGGGATTGACGCTGCCTGGG
TTCCCAGCATTGGCAGGACTTCAGAATGCCGTGGGCTCCAGCGAAGAAAAGGCTGCTGAC
AAGGCTGAGGGAGGACCCTTTAAAGATGGAGAGACCCTTGAAGGCAGCGATGCCGAGGAG
AGCCTGGATAAGACTGCAGAGTCCTCCCTCTTAGAAGACGAAATAGCACAGGGTGAAGAG
CTAGACTCACTTGATGGGGGGGATGAAATAGAAAACAATGAAAATGATGAATTG
Protein sequence predicted based on nucleotide sequence
MADPGMMSLFGEDGNIFSEGLEGLGECGYPENPVNPMGQQMPIDQGFASLQPSLHHPSTN QNQTKLTHFDHYNQYEQQKMHLMDQPNRMMSNTPGNGLASPHSQYHTPPVPQVPHGGSGG GQMGVYPGMQNERHGQSFVDSSSMWGPRAVQVPDQIRAPYQQQQPQPQPPQPAPSGPPAQ GHPQHMQQMGSYMARGDFSMQQHGQPQQRMSQFSQGQEGLNQGNPFIATSGPGHLSHVPQ QSPSMAPSLRHSVQQFHHHPSTALHGESVAHSPRFSPNPPQQGAVRPQTLNFSSRSQTVP SPTINNSGQYSRYPYSNLNQGLVNNTGMNQNLGLTNNTPMNQSVPRYPNAVGFPSNSGQG LMHQQPIHPSGSLNQMNTQTMHPSQPQGTYASPPPMSPMKAMSNPAGTPPPQVRPGSAGI PMEVGSYPNMPHPQPSHQPPGAMGIGQRNMGPRNMQQSRPFIGMSSAPRELTGHMRPNGC PGVGLGDPQAIQERLIPGQQHPGQQPSFQQLPTCPPLQPHPGLHHQSSPPHPHHQPWAQL HPSPQNTPQKVPVHQHSPSEPFLEKPVPDMTQVSGPNAQLVKSDDYLPSIEQQPQQKKKK KKNNHIVAEDPSKGFGKDDFPGGVDNQELNRNSLDGSQEEKKKKKRSKAKKDPKEPKEPK EKKEPKEPKTPKAPKIPKEPKEKKAKTATPKPKSSKKSSNKKPDSEASALKKKVNKGKTE GSENSDLDKTPPPSPPPEEDEDPGVQKRRSSRQVKRKRYTEDLEFKISDEEADDADAAGR DSPSNTSQSEQQESVDAEGPVVEKIMSSRSVKKQKESGEEVEIEEFYVKYKNFSYLHCQW ASIEDLEKDKRIQQKIKRFKAKQGQNKFLSEIEDELFNPDYVEVDRIMDFARSTDDRGEP VTHYLVKWCSLPYEDSTWERRQDIDQAKIEEFEKLMSREPETERVERPPADDWKKSESSR EYKNNNKLREYQLEGVNWLLFNWYNMRNCILADEMGLGKTIQSITFLYEIYLKGIHGPFL VIAPLSTIPNWEREFRTWTELNVVVYHGSQASRRTIQLYEMYFKDPQGRVIKGSYKFHAI ITTFEMILTDCPELRNIPWRCVVIDEAHRLKNRNCKLLEGLKMMDLEHKVLLTGTPLQNT VEELFSLLHFLEPSRFPSETTFMQEFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAP KEETIIEVELTNIQKKYYRAILEKNFTFLSKGGGQANVPNLLNTMMELRKCCNHPYLING AEEKILEEFKETHNAESPDFQLQAMIQAAGKLVLIDKLLPKLKAGGHRVLIFSQMVRCLD ILEDYLIQRRYPYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADT CIIFDSDWNPQNDLQAQARCHRIGQSKSVKIYRLITRNSYEREMFDKASLKLGLDKAVLQ SMSGRENATNGVQQLSKKEIEDLLRKGAYGALMDEEDEGSKFCEEDIDQILLRRTHTITI ESEGKGSTFAKASFVASGNRTDISLDDPNFWQKWAKKAELDIDALNGRNNLVIDTPRVRK QTRLYSAVKEDELMEFSDLESDSEEKPCAKPRRPQDKSQGYARSECFRVEKNLLVYGWGR WTDILSHGRYKRQLTEQDVETICRTILVYCLNHYKGDENIKSFIWDLITPTADGQTRALV NHSGLSAPVPRGRKGKKVKAQSTQPVVQDADWLASCNPDALFQEDSYKKHLKHHCNKVLL RVRMLYYLRQEVIGDQADKILEGADSSEADVWIPEPFHAEVPADWWDKEADKSLLIGVFK HGYEKYNSMRADPALCFLERVGMPDAKAIAAEQRGTDMLADGGDGGEFDREDEDPEYKPT RTPFKDEIDEFANSPSEDKEESMEIHATGKHSESNAELGQLYWPNTSTLTTRLRRLITAY QRSYKRQQMRQEALMKTDRRRRRPREEVRALEAEREAIISEKRQKWTRREEADFYRVVST FGVIFDPVKQQFDWNQFRAFARLDKKSDESLEKYFSCFVAMCRRVCRMPVKPDDEPPDLS SIIEPITEERASRTLYRIELLRKIREQVLHHPQLGERLKLCQPSLDLPEWWECGRHDRDL LVGAAKHGVSRTDYHILNDPELSFLDAHKNFAQNRGAGNTSSLNPLAVGFVQTPPVISSA HIQDERVLEQAEGKVEEPENPAAKEKCEGKEEEEETDGSGKESKQECEAEASSVKNELKG VEVGADTGSKSISEKGSEEDEEEKLEDDDKSEESSQPEAGAVSRGKNFDEESNASMSTAR DETRDGFYMEDGDPSVAQLLHERTFAFSFWPKDRVMINRLDNICEAVLKGKWPVNRRQMF DFQGLIPGYTPTTVDSPLQKRSFAELSMVGQASISGSEDITTSPQLSKEDALNLSVPRQR RRRRRKIEIEAERAAKRRNLMEMVAQLRESQVVSENGQEKVVDLSKASREATSSTSNFSS LSSKFILPNVSTPVSDAFKTQMELLQAGLSRTPTRHLLNGSLVDGEPPMKRRRGRRKNVE GLDLLFMSHKRTSLSAEDAEVTKAFEEDIETPPTRNIPSPGQLDPDTRIPVINLEDGTRL VGEDAPKNKDLVEWLKLHPTYTVDMPSYVPKNADVLFSSFQKPKQKRHRCRNPNKLDINT LTGEERVPVVNKRNGKKMGGAMAPPMKDLPRWLEENPEFAVAPDWTDIVKQSGFVPESMF DRLLTGPVVRGEGASRRGRRPKSEIARAAAAAAAVASTSGINPLLVNSLFAGMDLTSLQN LQNLQSLQLAGLMGFPPGLATAATAGGDAKNPAAVLPLMLPGMAGLPNVFGLGGLLNNPL SAATGNTTTASSQGEPEDSTSKGEEKGNENEDENKDSEKSTDAVSAADSANGSVGAATAP AGLPSNPLAFNPFLLSTMAPGLFYPSMFLPPGLGGLTLPGFPALAGLQNAVGSSEEKAAD KAEGGPFKDGETLEGSDAEESLDKTAESSLLEDEIAQGEELDSLDGGDEIENNENDEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
