Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878879
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4674nts
Open reading frame: 1 to 4674
ATGCAGCGAATGATCCAACAATTTGCTGCTGAATATACCTCAAAAAATAGCTCTACTCAG
GACCCCAGCCAGCCCAATAGCACAAAGAACCAAAGCCTGCCGAAAGCATCTCCAGTCACC
ACCTCTCCCACGGCTGCAACTACTCAGAACCCTGTGCTCAGCAAACTTCTCATGGCTGAC
CAAGACTCACCTCTGGACCTTACTGTCAGAAAGTCTCAGTCAGAACCTAGCGAACAAGAC
GGTGTACTTGATCTGTCCACTAAGAAAAGTCCATGTGCTGGCAGCACTTCCCTGAGCCAC
TCTCCAGGCTGCTCCAGTACTCAAGGGAACGGTGAGAACTCAACAGAGGCAAAAGCAGTA
GATTCTAACAATCAGTCGAAGTCCCCACTGGAGAAATTTATGGTCAAACTGTGTACTCAT
CATCAAAAGCAATTCATTCGTGTTCTGAACGACCTGTACACTGAATCTCAACCAGGCACT
GAGGACCTGCAGCCTTCTGATTCGGGAGCAATGGATGTATCCACTTGCAATGCTGGCTGT
GCCCAGCTCAGCACCAAACATAAGGAAAAAGATGCTCTGTGTCTCGATATGAAGTCTTCT
GCTTCTGTAGATTTGTTCGTAGACTCGTCAGACTCTCACAGCCCTCTACACTTGACGGAA
CAGACCCCGAAGAAGCCTCCTCCTGAGATAAACCCTGTAGATGGAAGAGAGAATGCCTTG
ACTGTTGTCCAGAAAGATTCCTCTGAACTTCCAACCACTAAATCGAATTCTATTAATAGC
AGTTCAGTGGATAGTTTCACTCCGGGATACCTCACTGCATCTAATTGTTCCTCAGTGAAC
TTCCACCACATCCCTAAAATCTTGGAGGGGCAGACCACTGGACAAGAGCAAGACACAAAT
GTGAACATATGTGAGGATGGTAAAGACCATATGCAGAGTTCAGCTTTAGTAGAAAGTCTA
ATTACAGTAAAAATGGCAGCTGAGAATAGTGAGGAAGGCAATACCTGTATTATTCCTCAA
AGAAATTTGTTCAAAGCTTTATCAGAAGAGGCTTGGAACTCAGGGTTTATGGGGAACTCA
TCTAGAACTGCTGACAAAGAGAATACTTTACAGTGTCCAAAAACACCTTTGCGCCAGGAT
TTAGAGGCAAATGAACAAGATGCAAGGCCAAAGCAAGAGAACCATCTTCACTCTCTGGGA
AGAAATAAGGTGGGTTACCATTTACATCCCAGTGATAAGGGCCAGTTTGATCATTCCAAA
GATGGTTGGTTAGGCCCCGGCCCTATGCCAGCTGTACACAAAGCGGCAAATGGACACTCA
AGAACCAAGATGATATCAACCTCCATCAAGACAGCTCGGAAAAGTAAAAGGGCATCAGGG
CTGAGGATAAATGATTATGATAACCAGTGTGATGTTGTTTATATCAGTCAACCAATAACA
GAATGCCACTTTGAGAATCAAAAATCAATATTATCTTCTCGGAAAACAGCCAGAAAGAGT
ACTCGAGGATACTTTTTCAATGGTGACTGTTGTGAGCTGCCAACTGTTCGTACACTGGCC
AGAAATTTACACTCCCAGGAAAAAGCAAGCTGCTCAGCATTGGCATCAGAGGCAGTTTTC
ACTCCTAAGCAGACCCTTACAATTCCAGCCCCTAGACATACAGTAGATGTGCAGCTTCCC
AGAGAAGACAACCCTGAAGAACCTAGCAAGGAAATCACCTCTCACGAGGAAGGAGGTGGA
GACGTTTCACCTCGAAAAGAACCTCAAGAGCCTGAGGTTTGCCCCACAAAGATTAAGCCG
AACCTGAGCAGCTCCCCTAGGTCAGAGGAAACGACAGCCTCCAGCCTGGTGTGGCCTCTC
CCTGCTCACCTTCCTGAAGAGGACCTGCCAGAAGGTGGCTCCACAGTCTCAGCTCCCACA
GCAAGTGGGATGTCTTCTCCTGAACACAACCAACCACCAGTTGCACTGTTGGATACGGAG
GAGATGAGTGTACCCCAGGACTGTCACCTCCTTCCCTCCACTGAAAGCTTTTCCGGGGGA
GTCAGTGAAGATGTCATTTCTAGGCCTCATTCTCCTCCTGAAATAGTCAGTAGAGAAGAA
AGTCCTCAGTGCTCAGAAAATCAGAGTTCCCCAATGGGCTTGGAGCCCCCCATGAGTCTG
GGAAAGGCTGAGGACAACCAAAGCATCAGTGCTGAGGTTGAGTCTGGAGACACCCAGGAG
CTAAATGTCGACCCACTCTTGAAGGAAAGCAGCACTTTTACTGATGAAAACCCCAGTGAA
ACTGAGGAAAGTGAGGCAGCAGGTGGTATAGGAAAATTAGAGGGAGAGGACGGTGATGTA
AAATGCCTGTCAGAAAAAGACACGTATGATACAAGCATTGACTCACTCGAAGAGAATTTG
GACAAGAAGAAAAAAGGTAAAAAATTCCCTGAGGCCTCTGATAGGTGCCTAAGAAGTCAA
CTTTCGGATTCTTCCTCTGCTGACAGATGCCTAAGAAATCAGAGTTCAGATTCTTCCTCA
GCTTGTCTTGAAATCAAAGTTCCTAAAAATCCTAGTGCAAAACGTTCAAAAAAAGAAGGG
CACCCTGGTGGGACAACACCTAAGGGCCTTCTACCTGACAGTTTCCACACGGAAACTCTG
GAGGACACAGAAAAGCCAAGTGTCAATGAACGCCCCTCTGAGAAAGATGCTGAGCAGGAG
GGCGAAGGCGGGGGGATCATCACCAGGCAGACTTTGAAAAACATGCTGGACAAAGAAGTC
AAGGAGTTACGAGGAGAGATTTTCCCCAGCAGGGACCCCATAACCACAGCTGGACAGCCA
CTGCCTGGAGAGAGATTGGAAATCTATGTTCAGTCTAAAATGGATGAGAAGAATGCTCAT
ATCCCCTCAGAAAGTATTGCTTGTAAGAGGGACCCAGAACAGGCAAAAGAAGAGCCAGGG
CATATTCCCACACAGCATGTGGAGGAGGCTGTGAATGAGGTAGACAACGAAAACACCCAG
CAGAAAGATGATGAGAGTGATGCCCCATGCAGCTCTCTTGGGTTGTCGAGTAGTGGAAGT
GGTGATGCTGCTAGGGCACCAAAATCGGTGCCAAGGCCTAAAAGATTGACCTCTTCAACC
TACAACCTAAGACACGCTCATTCTCTGGGCTCCTTGGATGCTTCAAAAGTGACTTCAGAA
AAGGAAGCTGCACAAGTAAACCCCATAATGCCAAAGGAAAATGGAGCTTCAGAGAGTGGA
GACCCCCTAGATGAGGACGATGTTGACACCGTGGTAGATGAACAGCCAAAGTTTATGGAA
TGGTGTGCTGAGGAGGAGAACCAAGAGCTCATCGCCAACTTCAATGCCCAGTACATGAAA
GTTCAGAAGGGCTGGATCCAGTTGGAGAAAGAAGGACAGCCAACACCAAGAGCAAGGAAC
AAATCAGATAAACTGAAAGAGATTTGGAAAAGCAAGAAAAGGTCACGGAAATGTAGGAGT
TCATTGGAGAGTCAGAAGTGTTCTCCTGTTCAGATGCTCTTTATGACAAACTTTAAATTA
TCTAATGTTTGTAAATGGTTCTTAGAGACAACTGAAACCCGGTCTCTAGTCATTGTGAAG
AAGCTCAATACTCGCCTTCCAGGAGACGTTCCCCCTGTCAAGCATCCTCTTCAGAAATAC
GCTCCTTCCAGCCTATATCCCAGTTCACTACAGGCTGAGCGCTTGAAAAAGCACTTGAAG
AAATTTCCTGGAGCTACCCCTGCTAAGAATAATTGGAAAATGCAGAAGCTCTGGGCCAAA
TTTCGAGAGAATCCTGATCAAGTGGAGCCAGAAGATGGCAGTGATGTCAGCCCCGGCCCT
AATTCTGAAGACAGCATAGAGGAAGTCAAGGAAGATAGAAACAGTCATCCTCCAGCAAAC
CTGCCCACTCCAGCCAGTACCCGGATTCTTAGAAAATATTCCAATATTCGAGGAAAGCTC
AGAGCCCAGCAACGTTTAATCAAGAATGAGAAAATGGAATGCCCAGATGCTCTGGCTGTG
GAAAGTAAGCCAAGTCGTAAGAGCGTATGCATCAACCCTCTGATGTCCCCCAAGCTTGCC
CTGCAAGTGGATGCAGATGGGTTTCCTGTTAAGCCCAAGAGTACTGAAGGAATGAAGGGA
AGGAAGGGGAAGCAGGTGTCTGAAATCTTGCCTAAAGCAGAAGTTCAGAGTAAACGCAAG
AGAACAGAAGGCAGCAGCCCTCCAGATAGTAAGAACAAGGGGCCTACGGTGAAAGCCAGC
AAAGAAAAGCATGCTGATGGAGCCACCAAAACCCCTGCTGCCAAGAGGCCAGCTGCAAGG
GACAGAAGCAGCCAACCCCCCAAAAAGACGTCTTTGAAAGAGAATAAAGTGAAGATCCCT
AAAAAGTCCGCTGGGAAGAGCTGCCCTCCCTCCAGGAAAGAAAAAGAGAATACAAACAAA
AGGCCTTCCCAGTCTATTGCCTCGGAAACACTGACGAAACCTGCAAAACAGAAGGGGGCC
GGTGAATCCTCTTCAAGGCCTCAGAAAGCCACGAATAGGAAGCAGAGTAGTGGAAAGACT
CGGGCCAGACCCTCAACGAAAACCCCAGAGAGCAGTGCAGCTCAGAGAAAGCGAAAGCTG
AAGGCAAAGCTGGACTGTTCGCACAGCAAACGGAGGCGGCTGGATGCAAAGTTG
Protein sequence predicted based on nucleotide sequence
MQRMIQQFAAEYTSKNSSTQDPSQPNSTKNQSLPKASPVTTSPTAATTQNPVLSKLLMAD QDSPLDLTVRKSQSEPSEQDGVLDLSTKKSPCAGSTSLSHSPGCSSTQGNGENSTEAKAV DSNNQSKSPLEKFMVKLCTHHQKQFIRVLNDLYTESQPGTEDLQPSDSGAMDVSTCNAGC AQLSTKHKEKDALCLDMKSSASVDLFVDSSDSHSPLHLTEQTPKKPPPEINPVDGRENAL TVVQKDSSELPTTKSNSINSSSVDSFTPGYLTASNCSSVNFHHIPKILEGQTTGQEQDTN VNICEDGKDHMQSSALVESLITVKMAAENSEEGNTCIIPQRNLFKALSEEAWNSGFMGNS SRTADKENTLQCPKTPLRQDLEANEQDARPKQENHLHSLGRNKVGYHLHPSDKGQFDHSK DGWLGPGPMPAVHKAANGHSRTKMISTSIKTARKSKRASGLRINDYDNQCDVVYISQPIT ECHFENQKSILSSRKTARKSTRGYFFNGDCCELPTVRTLARNLHSQEKASCSALASEAVF TPKQTLTIPAPRHTVDVQLPREDNPEEPSKEITSHEEGGGDVSPRKEPQEPEVCPTKIKP NLSSSPRSEETTASSLVWPLPAHLPEEDLPEGGSTVSAPTASGMSSPEHNQPPVALLDTE EMSVPQDCHLLPSTESFSGGVSEDVISRPHSPPEIVSREESPQCSENQSSPMGLEPPMSL GKAEDNQSISAEVESGDTQELNVDPLLKESSTFTDENPSETEESEAAGGIGKLEGEDGDV KCLSEKDTYDTSIDSLEENLDKKKKGKKFPEASDRCLRSQLSDSSSADRCLRNQSSDSSS ACLEIKVPKNPSAKRSKKEGHPGGTTPKGLLPDSFHTETLEDTEKPSVNERPSEKDAEQE GEGGGIITRQTLKNMLDKEVKELRGEIFPSRDPITTAGQPLPGERLEIYVQSKMDEKNAH IPSESIACKRDPEQAKEEPGHIPTQHVEEAVNEVDNENTQQKDDESDAPCSSLGLSSSGS GDAARAPKSVPRPKRLTSSTYNLRHAHSLGSLDASKVTSEKEAAQVNPIMPKENGASESG DPLDEDDVDTVVDEQPKFMEWCAEEENQELIANFNAQYMKVQKGWIQLEKEGQPTPRARN KSDKLKEIWKSKKRSRKCRSSLESQKCSPVQMLFMTNFKLSNVCKWFLETTETRSLVIVK KLNTRLPGDVPPVKHPLQKYAPSSLYPSSLQAERLKKHLKKFPGATPAKNNWKMQKLWAK FRENPDQVEPEDGSDVSPGPNSEDSIEEVKEDRNSHPPANLPTPASTRILRKYSNIRGKL RAQQRLIKNEKMECPDALAVESKPSRKSVCINPLMSPKLALQVDADGFPVKPKSTEGMKG RKGKQVSEILPKAEVQSKRKRTEGSSPPDSKNKGPTVKASKEKHADGATKTPAAKRPAAR DRSSQPPKKTSLKENKVKIPKKSAGKSCPPSRKEKENTNKRPSQSIASETLTKPAKQKGA GESSSRPQKATNRKQSSGKTRARPSTKTPESSAAQRKRKLKAKLDCSHSKRRRLDAKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
