Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878888
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6987nts
Open reading frame: 1 to 6987
ATGACATCGGCCACAGAGTTTGAAAATGTGGGCAACCAGCCACCTTACAGCAGGATCAAT
GCCCGCTGGGACGCCCCAGACGACGAGCTGGATAATGACAACAGCTCAGCCAGGCTCTTT
GAGAGGTCCCGGATAAAGGCCTTGGCAGATGAGCGGGAAGTTGTTCAGAAAAAGACCTTC
ACGAAATGGGTGAACTCGCACCTGGCTCGAGTGTCCTGCCGCATCACCGATCTCTACAAG
GACCTGCGGGATGGGCGCATGCTCATCAAGCTGCTGGAGGTGCTCTCTGGAGAGATGCTG
CCAAAGCCCACCAAGGGGAAGATGCGCATCCACTGCCTGGAGAATGTGGACAAGGCTCTC
CAGTTCCTCAAGGAGCAGCGTGTACACCTGGAGAACATGGGCTCCCACGACATTGTAGAT
GGCAACCACCGCCTGGTCCTGGGCCTCATCTGGACCATCATCCTCCGCTTCCAGATTCAG
GACATTGTGGTCCAAACTCAGGAAGGTCGTGAAACACGCTCAGCCAAGGATGCGTTGCTG
TTGTGGTGTCAGATGAAGACGGCAGGCTACCCTCATGTTAATGTCACCAACTTTACCTCC
AGCTGGAAGGATGGCTTGGCCTTTAATGCCCTGATACACAAGCACCGGCCCGACCTGATC
GACTTTGATAAGCTGAAGGACTCCAATGCCCGGCACAACCTGGAGCACGCATTCAATGTG
GCTGAGCGCCAGCTGGGCATCATCCCGCTCCTCGACCCCGAAGATGTCTTTACGGAAAAC
CCTGATGAGAAATCCATCATCACCTATGTGGTGGCCTTTTACCACTACTTCTCCAAGATG
AAGGTGCTGGCAGTGGAGGGCAAGCGTGTCGGCAAGGTTATTGACCATGCCATTGAGACT
GAGAAGATGATTGAAAAGTACAGCGGGCTAGCCTCGGACCTGCTCACCTGGATCGAGCAG
ACCATCACTGTCCTGAACAGCCGCAAGTTTGCCAACTCGCTGACGGGCGTCCAGCAGCAG
CTGCAGGCCTTCAGCACCTACCGCACCGTGGAGAAGCCGCCCAAGTTTCAAGAGAAGGGG
AATCTGGAAGTTCTACTTTTTACCATCCAGTCCCGGATGAGAGCCAACAATCAGAAAGTG
TACACACCCCACGATGGGAAACTAGTGTCTGACATCAACAGGGCCTGGGAAAGCCTGGAG
GAAGCTGAGTATCGGCGGGAGCTGGCCCTGAGAAATGAGCTCATTCGGCAGGAGAAGCTA
GAGCAACTGGCCCGGCGCTTTGACCGGAAGGCCGCAATGAGAGAGACCTGGCTCAGTGAA
AACCAGCGCCTCGTGGCCCAGGATAACTTTGGGTATGACCTGGCAGCTGTGGAGGCCGCC
AAGAAGAAGCATGAGGCCATCGAGACCGACACGGCTGCCTACGAGGAGCGGGTGAGAGCC
CTGGAGGACCTGGCTCAGGAGCTGGAGAAAGAGAACTACCATGACCAGAAGCGCATCACG
GCCCGCAAGGACAATATACTGCGCCTATGGAGCTACCTGCAGGAGCTGCTGCAGTCCCGG
CGCCAGAGGCTCGAGACCACCCTGGCACTGCAGAAGCTCTTCCAGGACATGCTGCACAGC
ATCGACTGGATGGATGAGATCAAGGCTCACCTCTTGTCTGCCGAGTTTGGGAAGCACTTG
TTGGAGGTTGAAGACCTGCTACAGAAGCACAAGTTGATGGAAGCTGACATCGCCATCCAA
GGGGACAAAGTGAAGGCCATCACCGCAGCCACCCTGAAGTTCACCGAGGGGAAAGGGTAC
CAGCCTTGTGACCCCCAGGTCATCCAGGACCGCATCAGCCACTTGGAGCAGTGCTTTGAG
GAGCTGAGCAACATGGCAGCTGGGCGGAAGGCCCAACTGGAGCAGTCCAAACGACTCTGG
AAGTTCTTCTGGGAGATGGATGAGGCTGAGAGCTGGATCAAGGAGAAGGAGCAGATCTAT
TCTTCCCTGGACTATGGCAAAGACCTGACCAGTGTGCTCATCTTACAGCGCAAGCACAAG
GCCTTTGAGGATGAGCTCCGTGGGCTGGATGCTCACCTGGAGCAGATCTTCCAGGAGGCT
CATGGCATGGTTGCGCGCAAGCAGTTTGGGCACCCGCAGATCGAGGCCCGCATAAAGGAG
GTGTCGGCACAGTGGGACCAGCTGAAGGACCTGGCTGCCTTCTGCAAGAAGAACCTCCAG
GATGCTGAGAACTTTTTCCAGTTCCAGGGCGATGCGGATGACCTGAAGGCTTGGCTGCAA
GACGCCCACCGGCTGCTCTCTGGTGAAGATGTGGGGCAGGACGAAGGGGCCACGCGGGCC
CTGGGGAAAAAGCACAAGGACTTCCTGGAGGAGCTGGAGGAGAGCCGTGGGGTGATGGAG
CACCTGGAGCAGCAGGCCCAGGGATTCCCCGAAGAGTTTCGGGATTCCCCAGATGTGACC
CATCGGCTGCAGGCCCTGCGGGAGCTCTACCAACAGGTGGTGGCCCAGGCGGACCTGCGT
CAGCAGAGGCTGCAGGAAGCCCTGGACCTGTACACGGTGTTCGGGGAGACAGACGCCTGT
GAGCTGTGGATGGGAGAGAAGGAGAAGTGGCTGGCCGAGATGGAAATGCCAGACACCCTG
GAGGACCTGGAGGTCGTGCAGCACAGGTTCGACATCCTGGACCAGGAGATGAAGACCTTG
ATGACTCAGATTGATGGTGTGAACCTCGCTGCCAACAGCTTGGTAGAGAGTGGCCACCCA
CGCAGCAGGGAGGTGAAGCAGTACCAGGACCATCTGAACACCAGGTGGCAGGCATTTCAG
ACCCTGGTGTCGGAGCGGCGGGAGGCTGTGGACTCAGCCCTCCGAGTGCACAACTACTGC
GTAGATTGCGAGGAGACCAGCAAGTGGATCACGGACAAGACAAAGGTAGTGGAGTCCACA
AAAGACCTGGGGCGGGACCTGGCAGGTATCATCGCCATCCAGAGGAAGTTGTCAGGGCTG
GAGCGTGACGTGGCCGCCATCCAGGCCCGTGTGGATGCCCTGGAGCGTGAGTCCCAGCAG
CTGATGGACTCGCACCCTGAGCAGAAGGAGGATATTGGTCAGCGGCAAAAACACTTGGAG
GAGCTGTGGCAGGGCCTGCAGCAATCCCTGCAGGGCCAGGAGGACTTGCTGGGGGAAGTC
AGCCAGCTGCAGGCCTTCCTGCAGGATCTGGATGACTTCCAGGCCTGGCTCTCCATCACC
CAGAAGGCTGTGGCCTCTGAGGACATGCCCGAATCCCTCCCAGAGGCTGAGCAGCTCCTG
CAGCAGCATGCAGGTATCAAGGATGAGATTGACGGGCACCAAGACAGCTACCAGCGTGTT
AAGGAGTCTGGGGAGAAAGTGATCCAAGGCCAGACGGACCCAGAGTATCTGCTTCTGGGC
CAGCGGCTGGAGGGCCTGGATACTGGCTGGAATGCCCTGGGCAGGATGTGGGAGAGCCGC
AGCCACACCCTCGCTCAGTGCCTTGGCTTCCAGGAGTTCCAGAAAGATGCCAAGCAGGCT
GAAGCCATCCTCAGCAACCAGGAATACACTCTGGCTCACTTGGAGCCCCCAGACTCCCTG
GAAGCTGCAGAGGCTGGGATCCGGAAGTTTGAGGATTTCTTGGGGTCTATGGAGAACAAC
CGGGATAAGGTCTTGAGTCCTGTGGACTCTGGAAACAAGCTGGTAGCTGAGGGAAACCTA
TACTCAGACAAGATCAAGGAGAAGGTGCAGCTGATTGAGGACAGGCACAGGAAGAACAAC
GAGAAGGCCCAGGAGGCCTCTGTCCTACTGAGAGACAACCTGGAGCTACAGAACTTCCTC
CAGAACTGCCAGGAGCTCACTCTCTGGATCAACGACAAGCTGCTGACATCTCAGGATGTC
TCCTATGATGAAGCACGAAACCTTCACAATAAATGGCTAAAGCACCAGGCGTTTGTGGCA
GAGCTGGCTTCCCATGAAGGGTGGCTAGAGAACATCGATGCGGAAGGAAAGCAGCTGATG
GATGAGAAGCCCCAGTTTACAGCCCTGGTGTCCCAAAAGCTGGAAGCCCTGCACCGGCTC
TGGGACGAGCTGCAGGCCACCACAAAGGAGAAGACCCAGCACCTCTCGGCTGCCAGGAGC
TCCGACCTGCGCTTGCAGACCCATGCTGACCTCAACAAGTGGATCAGCGCCATGGAGGAC
CAGCTGCGGTCAGACGACCCGGGCAAGGACCTGACCAGTGTCAATCGGATGTTGGCTAAG
CTGAAGCGAGTGGAGGACCAAGTGAATGTGCGAAAAGAGGAGCTGGGGGAGCTGTTTGCC
CAGGTGCCTTCAATGGGAGAGGAGGGAGGAGATGCAGACTTGAGCATCGAGAAGCGGTTC
CTGGACCTCCTGGAACCCCTAGGAAGGAGGAAGAAGCAGCTGGAATCATCCAGAGCCAAG
CTGCAGATCAGCCGGGACTTAGAGGATGAGACGCTTTGGGTGGAGGAGAGGCTGCCTCTG
GCCCAGTCAGCCGACTATGGCACTAATCTGCAAACTGTGCAACTGTTCATGAAGAAGAAC
CAGACACTGCAGAATGAGATTCTGGGCCATACGCCGCGGGTTGAGGATGTGCTGCAGAGA
GGGCAGCAGCTGGTGGAGGCGGCGGAGATCGACTGCCAGGACCTTGAGGAGCGCCTGGGG
CACCTGCAGAGCTCCTGGGACAGGCTGCGGGAGGCAGCGGCCGGGAGGCTGCAGCGACTG
AGGGACGCCAACGAGGCACAGCAGTACTACCTGGATGCAGACGAGGCTGAGGCCTGGATT
GGCGAGCAGGAGCTCTACGTCATCTCCGATGAGATCCCCAAGGATGAAGAGGGCGCCATT
GTGATGCTGAAGCGACATTTGCGGCAGCAGCGTGCGGTGGAGGACTACGGCCGGAACATC
AAGCAGCTGGCCAGCCGGGCCCAGGGCCTGCTGTCTGCAGGCCACCCTGAGGGGGAACAG
ATCATCAGACTTCAGGGGCAAGTGGACAAGCACTACGCAGGGCTGAAGGACGTGGCGGAA
GAGCGCAAGCGCAAGCTGGAGAACATGTACCACCTGTTCCAGCTCAAGCGGGAGACCGAC
GACCTGGAGCAGTGGATTTCAGAAAAGGAGCTAGTGGCCTCTTCCCCGGAAATGGGGCAA
GACTTTGACCACGTGACTCTTCTGCGGGACAAGTTCCGGGACTTTGCCCGGGAGACCGGG
GCGATTGGGCAGGAGCGGGTGGACAATGTGAATGCCTTCATCGAGCGACTCATCGACGCG
GGCCACAGCGAGGCGGCCACCATCGCCGAGTGGAAGGACGGGCTGAACGAGATGTGGGCA
GACCTCCTGGAGCTCATTGACACGCGCATGCAGCTGCTGGCCGCCTCCTATGACCTGCAC
CGCTACTTCTACACGGGTGCCGAGATCCTGGGCCTCATCGACGAGAAGCACCGCGAGCTG
CCCGAGGACGTGGGGCTGGACGCCAGCACGGCCGAGTCCTTCCACCGGGTGCACACAGCC
TTCGAGCGGGAGCTCCACCTGCTGGGTGTCCAGGTGCAGCAGTTCCAGGACGTGGCCACC
CGTCTGCAGACAGCATATGCTGGGGAGAAGGCAGAGGCCATCCAGAACAAGGAGCAGGAG
GTGTCTGCCGCGTGGCAGGCGCTGCTCGATGCCTGTGCCGGGCGCCGGACCCAGCTAGTG
GACACGGCGGATAAATTCCGCTTCTTCAGCATGGCCCGTGACCTCCTCTCCTGGATGGAG
AGCATCATCCGGCAGATCGAGACCCAGGAGAGGCCCAGGGATGTCTCCTCTGTGGAACTG
CTCATGAAGTATCACCAGGGCATCAATGCAGAGATTGAAACCCGGAGCAAGAACTTCAGT
GCCTGCCTGGAGCTTGGCGAGTCCCTGCTGCAGCGGCAGCACCAGGCCTCAGAGGAGATC
CGCGAGAAACTGCAGCAGGTGATGTCCAGGAGGAAAGAGATGAATGAGAAGTGGGAAGCC
CGCTGGGAGCGGCTCCGCATGTTGCTGGAGGTGTGCCAGTTCTCGAGGGATGCCTCTGTG
GCTGAGGCGTGGCTGATTGCCCAGGAGCCCTACCTGGCCAGCGGGGACTTTGGACACACA
GTGGACAGTGTGGAGAAGCTCATCAAGAGGCATGAGGCTTTTGAGAAGTCCACGGCCAGC
TGGGCAGAGCGCTTTGCTGCCCTGGAGAAGCCCACCACGCTTGAGCTGAAAGAACGCCAG
ATTGCAGAGAGACCCGCAGAGGAGACTGGGCCTCAAGAGGAGGAAGGCGAGACAGCAGGG
GAGGCTCCAGTTTCCCACCATGCGGCCACCGAGAGAACGTCCCCGGGGGAAGAAGAGGGA
ACGTGGCCTCAGAACCTGCAGCAGCCACCACCGCCGGGTCAGCACAAGGATGGGCAGAAA
TCCACTGGGGATGAGAGGCCCACCACGGAGCCCCTCTTTAAGGTCCTAGATACGCCTCTG
AGCGAGGGTGATGAGCCCGCAACGCTGCCGGCCCCGCGGGACCATGGGCAGAGTGTGCAG
ATGGAAGGCTACCTGGGCCGCAAGCATGACCTGGAGGGGCCCAACAAGAAGGCTTCCAAC
AGGTCCTGGAACAACCTGTACTGTGTGCTCAGGAACAGTGAGCTAACCTTCTACAAGGAT
GCCAAGAACCTGGCCCTGGGGATGCCCTACCATGGGGAGGAACCCCTGGCCCTGAGACAT
GCCATCTGTGAGATTGCTGCCAACTACAAGAAGAAGAAGCACGTCTTTAAGCTGAGGCTG
AGTAATGGCAGCGAGTGGCTCTTCCATGGCAAGGATGAGGAGGAGATGCTGTCCTGGCTG
CAGGGCGTGAGCACCGCCATCAACGAGTCCCAGAGCATCCGCGTCAAGGCGCAGAGCCTG
CCCCTGCCTTCCCTCTCCGGCCCCGACGCCAGCCTCGGCAAGAAAGACAAGGAGAAGAGA
TTCAGCTTCTTCCCCAAAAAGAAGTTG
Protein sequence predicted based on nucleotide sequence
MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTF TKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKAL QFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALL LWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNV AERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIET EKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKG NLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKL EQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRA LEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHS IDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGY QPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIY SSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKE VSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRA LGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLR QQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTL MTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYC VDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQ LMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSIT QKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLG QRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSL EAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNN EKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVA ELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARS SDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFA QVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPL AQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLG HLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAI VMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAE ERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETG AIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLH RYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVAT RLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWME SIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEI REKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHT VDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAG EAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPL SEGDEPATLPAPRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKD AKNLALGMPYHGEEPLALRHAICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWL QGVSTAINESQSIRVKAQSLPLPSLSGPDASLGKKDKEKRFSFFPKKKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
