Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00879079
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6039nts
Open reading frame: 1 to 6039
ATGTGGATACTGGCTCTCTCCTTGTTCCAGAGCTTCGCGAATGTTTTCAGTGAAGACCTA
CACTCCAGCCTCTACTTTGTCAATGCATCTCTGCAAGAGGTAGTGTTTGCCAGCACCACG
GGGACTCTGGTGCCCTGCCCCGCAGCAGGCATCCCTCCTGTGACTCTCAGATGGTACCTA
GCCACGGGCGAGGAGATCTACGATGTCCCCGGGATCCGCCACGTCCACCCCAACGGCACT
CTCCAAATTTTCCCCTTCCCTCCTTCAAGCTTCAGTACCTTAATCCATGATAATACTTAT
TATTGCACAGCTGAAAATCCTTCAGGGAAAATTAGAAGTCAGGATGTCCACATCAAGGCT
GTTTTACGGGAGCCCTATACAGTCCGTGTGGAGGACCAGAAAACCATGAGAGGCAATGTT
GCGGTCTTCAAGTGCATTATCCCCTCCTCGGTGGAGGCGTACATCACTGTCGTCTCATGG
GAGAAAGACACTGTTTCACTTGTCTCAGGATCTAGATTTCTCATCACATCCACGGGAGCC
TTGTATATTAAAGATGTACAGAATGAAGATGGATTGTATAACTACCGCTGCATCACGCGG
CATCGATACACCGGAGAGACGAGGCAGAGCAACAGCGCCAGACTTTTTGTATCAGACCCA
GCGAACTCAGCCCCATCCATACTGGATGGGTTTGACCATCGCAAAGCCATGGCTGGGCAG
CGTGTGGAGCTGCCTTGCAAAGCGCTCGGGCACCCTGAGCCAGATTACCGCTGGCTGAAG
GACAACATGCCCCTGGAACTTTCAGGGAGGTTCCAGAAGACCGTGACGGGGCTGCTCATT
GAGAACATTCGCCCCTCGGACTCAGGCAGCTATGTTTGTGAAGTGTCCAACAGATACGGA
ACTGCTAAGGTGATAGGCCGCCTGTACGTGAAACAGCCACTGAAAGCCACCATCAGTCCC
AGGAAGGTTAAAAGCAGCGTGGGTAGCCAAGTTTCCTTGTCCTGCAGCGTGACAGGAACT
GAGGACCAGGAACTCTCCTGGTACCGCAATGGTGAAATCCTCAACCCTGGAAAAAATGTG
AGGATCACAGGGATCAACCACGAAAACCTTATAATGGATCACATGGTCAAAAGTGACGGG
GGCGCATACCAGTGCTTTGTGCGCAAGGACAAGCTGTCCGCTCAAGACTATGTGCAGGTG
GTCCTTGAAGATGGAACTCCCAAAATTATTTCTGCCTTTAGTGAAAAGGTGGTGAGTCCA
GCAGAGCCGGTTTCCCTTATGTGCAACGTGAAGGGAACACCTTTGCCCACGATCACGTGG
ACCCTGGACGATGACCCGATTCTCAAGGGTGGCAGTCACCGCATCAGCCAGATGATCACG
TCGGAGGGGAACGTGGTCAGCTACCTGAACATCTCCAGCTCCCAGGTCCGGGACGGGGGA
GTCTACCGCTGCACTGCCAACAACTCGGCGGGAGTCGTCCTGTACCAGGCTCGAATAAAC
GTAAGAGGGCCTGCAAGCATTCGACCAATGAAAAACATCACAGCAATAGCAGGACGGGAC
ACATACATTCACTGTCGTGTGATTGGCTATCCGTATTACTCCATTAAATGGTACAAGAAC
TCTAACCTGCTTCCTTTCAACCACCGCCAAGTGGCATTTGAGAACAATGGAACTCTTAAA
CTTTCAGATGTGCAAAAGGAAGTGGACGAGGGGGAGTACACGTGCAACGTGTTGGTTCAA
CCACAACTCTCCACCAGCCAGAGCGTCCACGTGACCGTGAAAGTTCCGCCTTTCATACAA
CCCTTTGAGTTTCCAAGATTCTCCATTGGGCAGCGGGTCTTCATCCCCTGTGTTGTGGTC
TCAGGGGACTTACCCATCACGATCACCTGGCAGAAGGATGGCCGGCCAATCCCTGGGAGC
CTTGGGGTGACCATTGACAATATTGACTTCACGAGCTCCTTGAGGATTTCCAATCTCTCG
CTCATGCACAATGGGAATTACACCTGCATAGCCCGGAATGAGGCCGCCGCTGTGGAGCAC
CAAAGCCAGTTGATTGTCAGAGTTCCTCCCAAGTTTGTGGTTCAGCCACGGGACCAGGAC
GGGATTTATGGCAAAGCAGTCATCCTCAATTGTTCTGCTGAGGGTTACCCTGTACCTACC
ATCGTGTGGAAATTCTCTAAAGGTGCTGGGGTTCCCCAGTTCCAGCCAATTGCCCTAAAT
GGCCGAATCCAAGTTCTCAGCAATGGGTCGTTGCTGATCAAGCATGTCGTGGAGGAAGAC
AGTGGCTACTACCTCTGCAAGGTCAGCAACGATGTGGGCGCAGACGTCAGCAAGTCCATG
TACCTCACGGTTAAAATTCCTGCGATGATAACATCCTATCCAAATACTACCCTGGCCACG
CAGGGGCAGAAAAAGGAGATGAGCTGCACGGCGCATGGTGAGAAGCCCATTATAGTCCGC
TGGGAGAAGGAGGACCGAATCATTAACCCTGAGATGGCCCGTTATCTTGTGTCCACCAAG
GAGGTGGGAGAAGAGGTGATTTCTACTCTGCAGATTTTGCCAACTGTGAGAGAAGATTCT
GGTTTCTTTTCCTGCCATGCTATTAATTCTTATGGGGAGGACCGTGGAATAATTCAGCTC
ACAGTGCAAGAGCCCCCAGACCCTCCCGAAATTGAGATCAAAGATGTCAAAGCACGCACA
ATTACGCTCAGGTGGACCATGGGGTTTGATGGAAACAGTCCCATCACAGGCTACGATATT
GAATGCAAAAATAAATCAGACTCCTGGGATTCTGCTCAGAGAACCAAAGATGTTTCCCCT
CAGCTGAACTCGGCCACCATCATTGATATCCACCCTTCCTCCACCTACAGCATCCGCATG
TACGCCAAGAACCGGATTGGCAAGAGCGAGCCCAGCAACGAGCTCACCATCACGGCGGAC
GAGGCAGCTCCTGATGGTCCACCTCAGGAAGTTCACCTGGAGCCTATATCATCTCAGAGC
ATCAGGGTCACATGGAAGGCTCCCAAGAAACATTTGCAAAATGGGATTATCCGTGGCTAC
CAAATAGGTTACCGAGAGTACAGCACTGGGGGTAACTTCCAATTCAACATTATCAGTGTC
GACACCAGCGGGGACAGTGAGGTTTACACCCTGGACAACCTGAATAAGTTCACTCAGTAC
GGCCTGGTGGTGCAGGCCTGTAACCGGGCCGGCACGGGGCCTTCTTCTCAGGAAATCATC
ACCACCACTCTCGAGGATGTGCCCAGTTACCCCCCCGAAAATGTCCAAGCCATAGCAACA
TCACCAGAAAGCATATCAATATCCTGGTCCACACTTTCCAAGGAAGCCTTGAATGGAATT
CTCCAGGGGTTCAGAGTCATTTACTGGGCCAACCTCATGGACGGAGAGCTGGGTGAGATT
AAAAACATCACCACCACACAGCCTTCACTGGAGCTGGACGGGCTGGAAAAGTACACCAAC
TACAGCATCCAGGTGCTGGCCTTCACCCGCGCAGGAGACGGGGTCAGGAGTGAGCAGATC
TTCACCCGGACCAAAGAGGATGTTCCAGGTCCTCCCGCGGGTGTGAAGGCAGCGGCGGCC
TCAGCCTCCATGGTCTTTGTGTCCTGGCTTCCCCCTCTCAAGCTGAACGGCATCATCCGA
AAGTACACTGTATTCTGCTCCCACCCCTATCCCACAGTGATCAGCGAGTTTGAGGCCTCT
CCCGACTCGTTTTCCTACAGAATTCCCAACCTGAGTAGGAATCGTCAGTACAGCGTCTGG
GTGGTGGCTGTTACTTCAGCCGGAAGAGGCAACAGCAGTGAAATCATCACAGTCGAGCCA
CTAGCAAAAGCTCCTGCACGAATCCTGACCTTCAGTGGGACAGTGACTACTCCATGGATG
AAAGACATTGTCTTGCCTTGTAAGGCTGTTGGGGACCCTTCTCCTGCAGTCAAATGGATG
AAAGACAGTAACGGGACACCCAGTCTAGTAACGATTGATGGGCGGAGGAGCATCTTTAGC
AACGGAAGCTTCATTATTCGCACGGTGAAAGCAGAAGACTCCGGCTATTACAGCTGCATT
GCCAATAACAACTGGGGATCTGATGAAATTATTTTAAACTTACAAGTACAAGTTCCACCA
GATCAGCCTCGGCTTACAGTCTCCAAGACCACGTCTTCCTCCATCACCCTTTCTTGGCTC
CCTGGAGACAACGGGGGCAGCTCTATCAGAGGATACATACTGCAGTACTCCGAGGACAAT
AGTGAGCAGTGGGGGAGTTTTCCAATCAGCCCCAGCGAACGTTCCTATCGCTTGGAAAAT
CTCAAATGTGGGACTTGGTATAAGTTCACACTGACAGCCCAAAATGGAGTGGGCCCAGGG
CGCATAAGTGAAATCATAGAAGCAAAGACCTTAGGAAAAGAGCCCCAGTTCTCAAAGGAG
CAGGAGCTGTTTGCCAGCATCAACACCACACGCGTGAGGCTGAACCTCATTGGCTGGAAT
GATGGCGGCTGCCCCATCACCTCCTTCACACTAGAGTACAGGCCCTTTGGGACCACAGTT
TGGACCACAGCTCAGAGGACCTCTCTCTCCAAGTCCTACATCCTGTATGACCTGCAGGAA
GCCACCTGGTATGAGCTGCAGATGCGGGTGTGCAACAGTGCGGGCTGCGCGGAGAAGCAG
GCCAACTTCGCTACGCTGAACTACGATGGCAGTACAATTCCTCCACTCATTAAGTCAGTT
GTCCAAAACGAAGAAGGGCTGACGACCAACGAGGGGCTCAAGATGCTGGTGACCATCTCC
TGTATCCTGGTGGGGGTCTTGCTGCTGTTTGTGCTCCTGCTGGTTGTGCGGAGGAGGCGG
CGGGAGCAGAGGCTAAAGAGGCTGCGAGATGCAAAGAGTTTAGCTGAAATGCTCATGAGT
AAGAATACCCGGACTTCAGATACGTTAAGCAAGCAACAGCAGACCCTGCGAATGCACATC
GACATACCCAGGGCTCAGCTTTTGATTGAAGAGAGAGACACGATGGAGACCATTGATGAT
CGCTCCACGGTTCTGTTGACGGATGCTGACTTTGGAGAGGCAGCTAAGCAGAAGTCCCTG
ACGGTCACTCACACGGTCCATTACCAATCGGTGTCTCAGGCCACTGGGCCCTTAGTGGAT
GTTTCAGACGCTCGGCCGGGAACGAATCCCACCACCAGGAGGAATGCCAAGGCTGGGCCC
ACAGCGAGAAACCGCTATGCCAGCCAGTGGACCCTCAACCGACCCCACCCCACCATCTCA
GCACACACCCTCACCACAGACTGGAGGCTGCCAACACCCAGGGCTGCAGGATCAGTAGAC
AAAGAGAGCGACAGTTACAGCGTCAGCCCCTCGCAAGACACAGATCGAGCAAGAAGCAGC
ATGGTCTCCACAGAAAGTGCCTCCTCCACTTACGAAGAACTGGCCAGGGCCTACGAACAC
GCCAAGATGGAAGAGCAACTGAGGCACGCCAAGTTCACCATCACGGAGTGCTTCATATCA
GACACGTCATCGGAGCAGTTGACGGCAGGGACAAATGAGTACACGGACAGTCTGACCTCC
AGCACCCCTTCCGAATCGGGAATCTGCAGGTTCACTGCATCTCCCCCCAAACCTCAGGAT
GGAGGAAGAGTAATGAATATGGCAGTTCCAAAGGCACATCGGCCAGGTGACCTCATACAT
TTGCCTCCATACCTTAGAATGGACTTTTTGTTAAACCGAGGTGGTCCAGGCACCAGCAGG
GACCTGAGCTTAGGACAAGCATGCTTGGAACCTCAGAAAAGCCGGACCCTGAAGCGCCCC
ACGGTCCTGGAGCCCATCCCGATGGAAGCCGCCTCCTCCGCCTCCTCCACGAGAGAAGGA
CAGTCGTGGCAGCCGGGGGCCGTGGCCACATTACCTCAGCGGGAGGGAGCAGAGCTGGGA
CAGGCAGCTAAAATGAGCAGCTCCCAAGAATCACTGCTCGACTCCCGGGGCCATTTGAAA
GGAAACAATCCTTACGCAAAATCTTACACCCTGGTATTG
Protein sequence predicted based on nucleotide sequence
MWILALSLFQSFANVFSEDLHSSLYFVNASLQEVVFASTTGTLVPCPAAGIPPVTLRWYL ATGEEIYDVPGIRHVHPNGTLQIFPFPPSSFSTLIHDNTYYCTAENPSGKIRSQDVHIKA VLREPYTVRVEDQKTMRGNVAVFKCIIPSSVEAYITVVSWEKDTVSLVSGSRFLITSTGA LYIKDVQNEDGLYNYRCITRHRYTGETRQSNSARLFVSDPANSAPSILDGFDHRKAMAGQ RVELPCKALGHPEPDYRWLKDNMPLELSGRFQKTVTGLLIENIRPSDSGSYVCEVSNRYG TAKVIGRLYVKQPLKATISPRKVKSSVGSQVSLSCSVTGTEDQELSWYRNGEILNPGKNV RITGINHENLIMDHMVKSDGGAYQCFVRKDKLSAQDYVQVVLEDGTPKIISAFSEKVVSP AEPVSLMCNVKGTPLPTITWTLDDDPILKGGSHRISQMITSEGNVVSYLNISSSQVRDGG VYRCTANNSAGVVLYQARINVRGPASIRPMKNITAIAGRDTYIHCRVIGYPYYSIKWYKN SNLLPFNHRQVAFENNGTLKLSDVQKEVDEGEYTCNVLVQPQLSTSQSVHVTVKVPPFIQ PFEFPRFSIGQRVFIPCVVVSGDLPITITWQKDGRPIPGSLGVTIDNIDFTSSLRISNLS LMHNGNYTCIARNEAAAVEHQSQLIVRVPPKFVVQPRDQDGIYGKAVILNCSAEGYPVPT IVWKFSKGAGVPQFQPIALNGRIQVLSNGSLLIKHVVEEDSGYYLCKVSNDVGADVSKSM YLTVKIPAMITSYPNTTLATQGQKKEMSCTAHGEKPIIVRWEKEDRIINPEMARYLVSTK EVGEEVISTLQILPTVREDSGFFSCHAINSYGEDRGIIQLTVQEPPDPPEIEIKDVKART ITLRWTMGFDGNSPITGYDIECKNKSDSWDSAQRTKDVSPQLNSATIIDIHPSSTYSIRM YAKNRIGKSEPSNELTITADEAAPDGPPQEVHLEPISSQSIRVTWKAPKKHLQNGIIRGY QIGYREYSTGGNFQFNIISVDTSGDSEVYTLDNLNKFTQYGLVVQACNRAGTGPSSQEII TTTLEDVPSYPPENVQAIATSPESISISWSTLSKEALNGILQGFRVIYWANLMDGELGEI KNITTTQPSLELDGLEKYTNYSIQVLAFTRAGDGVRSEQIFTRTKEDVPGPPAGVKAAAA SASMVFVSWLPPLKLNGIIRKYTVFCSHPYPTVISEFEASPDSFSYRIPNLSRNRQYSVW VVAVTSAGRGNSSEIITVEPLAKAPARILTFSGTVTTPWMKDIVLPCKAVGDPSPAVKWM KDSNGTPSLVTIDGRRSIFSNGSFIIRTVKAEDSGYYSCIANNNWGSDEIILNLQVQVPP DQPRLTVSKTTSSSITLSWLPGDNGGSSIRGYILQYSEDNSEQWGSFPISPSERSYRLEN LKCGTWYKFTLTAQNGVGPGRISEIIEAKTLGKEPQFSKEQELFASINTTRVRLNLIGWN DGGCPITSFTLEYRPFGTTVWTTAQRTSLSKSYILYDLQEATWYELQMRVCNSAGCAEKQ ANFATLNYDGSTIPPLIKSVVQNEEGLTTNEGLKMLVTISCILVGVLLLFVLLLVVRRRR REQRLKRLRDAKSLAEMLMSKNTRTSDTLSKQQQTLRMHIDIPRAQLLIEERDTMETIDD RSTVLLTDADFGEAAKQKSLTVTHTVHYQSVSQATGPLVDVSDARPGTNPTTRRNAKAGP TARNRYASQWTLNRPHPTISAHTLTTDWRLPTPRAAGSVDKESDSYSVSPSQDTDRARSS MVSTESASSTYEELARAYEHAKMEEQLRHAKFTITECFISDTSSEQLTAGTNEYTDSLTS STPSESGICRFTASPPKPQDGGRVMNMAVPKAHRPGDLIHLPPYLRMDFLLNRGGPGTSR DLSLGQACLEPQKSRTLKRPTVLEPIPMEAASSASSTREGQSWQPGAVATLPQREGAELG QAAKMSSSQESLLDSRGHLKGNNPYAKSYTLVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
