Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00879088
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 9228nts
Open reading frame: 1 to 9228
ATGCGCGGGGGCGTGCTCCTGGTCTTGCTGCTGTGTGTCGCCGCGCAGTGCCGGCAGAGA
GGCCTGTTTCCTGCCATTCTCAATCTTGCCAGCAATGCTCACATCAGCACCAATGCCACC
TGTGGCGAGAAGGGGCCGGAGATGTTCTGCAAACTTGTGGAGCATGTGCCAGGTCGGCCC
GTCCGAAACCCACAGTGCCGGATCTGTGATGGCAACAGCGCAAACCCCAGAGAACGCCAT
CCAATATCACATGCCATAGATGGCACCAATAACTGGTGGCAAAGTCCCAGCATTCAGAAT
GGGAGAGAATATCACTGGGTCACAATCACTCTGGACTTAAGACAGGTCTTTCAAGTTGCA
TATGTCATCATTAAAGCTGCCAATGCCCCTCGACCTGGAAACTGGATTTTGGAGCGTTCT
CTGGATGGCACCACGTTCAGCCCCTGGCAGTATTATGCAGTCAGCGACTCAGAGTGTTTG
TCTCGTTACAATATAACTCCAAGACGAGGGCCACCCACCTACAGGGCTGATGATGAAGTG
ATCTGCACCTCCTATTATTCCAGATTGGTGCCACTTGAGCATGGAGAGATTCATACATCA
CTCATCAATGGCAGACCAAGCGCTGACGATCTTTCACCCAAGTTGTTGGAATTCACTTCT
GCACGATATATTCGCCTTCGCTTGCAACGCATTAGAACGCTCAATGCAGATCTCATGACC
CTTAGCCACCGGGAACCTAAAGAACTGGATCCTATTGTTACCAGACGCTATTATTATTCA
ATAAAGGACATTTCTGTTGGAGGCATGTGTATCTGCTATGGCCATGCTAGTAGCTGCCCA
TGGGATGAAACTACAAAGAAACTGCAGTGTCAATGTGAGCATAATACTTGCGGGGAGAGC
TGTAACAGGTGCTGTCCTGGGTACCATCAGCAGCCCTGGAGGCCGGGAACCGTGTCCTCC
GGCAATACATGTGAAGCATGTAATTGTCACAATAAAGCCAAAGACTGTTACTATGATGAA
AGTGTTGCAAAGCAGAAGAAAAGTTTGAATACTGCTGGACAGTTCAGAGGAGGAGGGGTT
TGCATAAATTGCTTGCAGAACACCATGGGAATCAACTGTGAAACCTGTATTGATGGATAT
TATAGACCACACAAAGTGTCTCCTTATGAGGATGAGCCTTGCCGCCCCTGTAATTGTGAC
CCTGTGGGGTCCCTCAGTTCTGTCTGTATTAAGGATGACCTCCATTCTGACTTACACAAT
GGGAAGCAGCCAGGTCAGTGCCCATGTAAGGAAGGTTATACAGGAGAAAAATGTGATCGC
TGCCAACTTGGCTATAAGGATTACCCGACCTGTGTCTCCTGTGGGTGCAACCCAGTGGGC
AGTGCCAGTGATGAGCCCTGCACAGGGCCCTGTGTTTGTAAGGAAAACGTTGAGGGGAAG
GCCTGTGATCGCTGCAAGCCAGGATTCTATAACTTGAAGGAAAAAAACCCCCGGGGCTGC
TCCGAGTGCTTCTGCTTTGGCGTTTCTGATGTCTGCAGCAGCCTCTCTTGGCCTGTTGGT
CAGGTAAACAGTATGTCCGGGTGGCTGGTCACCGACTTGATCAGTCCCAGGAAGATCCCG
TCTCAGCAAGATGCACTAGGCGGGCGCCATCAGGTCAGCATCAACAACACCGCGGTCATG
CAGAGACTGGCTCCCAAGTACTACTGGGCAGCCCCCGAGGCCTACCTTGGAAATAAGCTG
ACTGCGTTTGGCGGATTCCTGAAATACACGGTGTCCTACGATATTCCGGTAGAGACGGTA
GACAGTAACCTCATGTCGCATGCTGACGTCATCATTAAGGGAAACGGACTCACTTTAAGC
ACACAGGCTGAGGGTCTGTCATTGCAGCCTTATGAAGAGTACCTAAACGTGGTTAGACTT
GTGCCTGAAAACTTCCAAGATTTTCACAGCAAAAGGCAGATTGATCGTGACCAGCTGATG
ACTGTCCTTGCCAATGTGACACATCTTTTGATCAGAGCCAACTACAATTCTGCAAAAATG
GCTCTTTACAGGTTGGAGTCCGTCTCTCTGGACATAGCCAGCTCTAATGCCATCGACCTG
GTGGTGGCCGCTGATGTGGAGCACTGTGAATGTCCGCAAGGCTACACAGGGACCTCCTGT
GAGTCGTGCCTCTCTGGCTATTACCGCGTGGATGGAATACTCTTTGGAGGAATTTGTCAA
CCCTGTGAATGCCACGGCCATGCAGCTGAGTGTAATGTTCACGGCGTTTGCATTGCGTGT
GCGCACAACACCACCGGCGTCCACTGTGAGCAGTGCTTGCCCGGCTTCTACGGGGAGCCT
TCCCGAGGGACACCTGGGGACTGCCAGCCCTGCGCCTGCCCTCTCACCATAGCCTCCAAC
AATTTCAGCCCCACCTGCCACCTCAATGATGGAGATGAAGTGGTCTGTGACTGGTGTGCC
CCGGGCTACTCAGGAGCTTGGTGTGAGAGATGTGCAGATGGTTACTATGGAAACCCAACA
GTGCCTGGCGAATCTTGTGTTCCCTGTGACTGCAGCGGCAACGTGGACCCCTCGGAGGCT
GGTCACTGTGACTCAGTCACCGGGGAGTGCCTGAAGTGCCTGGGGAACACAGATGGCGCC
CACTGTGAAAGGTGTGCTGACGGGTTCTATGGGGACGCTGTGACAGCCAAGAACTGCCGC
GCCTGTGAATGCCATGTGAAAGGCTCCCATTCTGCCGTGTGCCATCTTGAGACCGGGCTC
TGTGACTGCAAACCAAACGTGACTGGACAGCAGTGTGACCAGTGCTTGCATGGCTATTAT
GGGCTGGACTCAGGCCATGGCTGCCGGCCCTGCAACTGCAGCGTGGCAGGCTCCGTGTCA
GATGGCTGCACGGATGAAGGCCAGTGTCACTGTGTCCCAGGTGTGGCAGGGAAAAGGTGT
GACAGGTGTGCCCATGGCTTCTACGCCTACCAGGATGGTAGCTGTACACCCTGTGACTGC
CCACACACTCAGAATACCTGCGACCCAGAAACTGGAGAGTGTGTCTGCCCCCCTCACACA
CAGGGTGTGAAGTGTGAAGAATGTGAGGATGGGCACTGGGGCTACGATGCGGAGGTGGGG
TGCCAGGCCTGCAATTGCAGTCTCGTGGGGTCGACTCATCATCGGTGCGATGTGGTCACC
GGCCATTGCCAGTGCAAGTCAAAATTTGGTGGCCGGGCCTGCGATCAGTGTTCCTTGGGT
TACAGAGACTTTCCCGACTGTGTTCCCTGTGACTGTGACCTGAGGGGGACGTCGGGGGAC
GCCTGCAACCTGGAGCAGGGTCTCTGCGGCTGTGTGGAGGAAACCGGGGCCTGCCCTTGC
AAGGAAAATGTCTTTGGTCCTCAGTGCAACGAATGTCGAGAGGGCACCTTCGCTCTCCGC
GCAGACAACCCCCTGGGCTGCAGCCCGTGCTTCTGCTCCGGGCTGTCCCACCTCTGCTCA
GAGCTGGAGGACTACGTGAGGACCCCAGTAACGCTGGGCTCCGATCAGCCTCTTCTGCGT
GTGGTTTCTCAGAGTAACTTGAGGGGCACGACCGAGGGGGTTTACTACCAGGCCCCCGAC
TTCCTGCTGGATGCCGCCACCGTCCGGCAGCACATCCGTGCAGAGCCGTTTTACTGGCGG
CTGCCGCAGCAGTTCCAAGGAGACCAGCTCATGGCCTATGGTGGCAAACTGAAGTACAGC
GTGGCCTTCTATTCTTTGGATGGCGTCGGCACCTCCAATTTTGAGCCTCAAGTTCTCATC
AAAGGTGGTCGGATCAGAAAGCAAGTCATTTACATGGATGCACCAGCCCCAGAGAATGGA
GTGAGACAGGAACAAGAAGTAGCAATGAGAGAGAATTTTTGGAAATATTTTAACTCTGTT
TCTGAAAAACCTGTCACGCGAGAGGATTTTATGTCTGTCCTCAGCGATATTGAGTACATC
CTCATCAAGGCATCGTATGGTCAAGGATTACAGCAGAGCAGAATCTCAGACATTTCAATG
GAGGTTGGCAGAAAGGCTGAAAAGCTGCACCCAGAAGAAGAGGTTGCATCTCTTTTAGAG
AATTGTGTCTGTCCTCCTGGCACTGTGGGATTCTCATGTCAGGACTGCGCCCCTGGGTAC
CACAGAGGGAAGCTCCCAGCAGGGAGTGACAGGGGACCACGCCCTCTGGTTGCTCCTTGT
GTTCCCTGCAGTTGCAACAACCACAGTGACACCTGTGACCCCAACACCGGGAAGTGTCTG
AACTGTGGCGATAACACAGCAGGTGACCATTGTGATGTGTGTACTTCTGGCTACTACGGG
AAGGTGACTGGCTCAGCAAGTGACTGTGCTCTGTGTGCCTGTCCTCACAGCCCTCCTGCC
AGTTTTAGTCCCACTTGTGTCTTGGAAGGGGACCACGATTTCCGTTGTGACGCCTGTCTC
CTGGGCTATGAAGGAAAACACTGTGAAAGGTGCTCCTCAAGCTATTATGGGAACCCTCAA
ACACCAGGTGGCAGTTGCCAGAAGTGTGACTGCAACCCGCACGGCTCTGTCCACGGTGAC
TGTGACCGCACATCTGGGCAGTGCGTTTGCAGGCTGGGGGCCTCGGGGCTCCGGTGCGAT
GAGTGTGAACCGAGGCACATTCTGATGGAAACAGATTGTGTTTCCTGTGATGATGAGTGT
GTAGGTGTGCTGCTGAATGACTTGGATGAGATTGGTGATGCCGTTCTTTCTCTGAACCTC
ACTGGCATTATCCCTGTCCCATATGGAATTTTGTCAAACCTGGAAAATACAACTAAATAT
CTCCAGGAATCTTTATTAAAAGAAAATATGCAAAAGGACCTGGGAAAAATTAAGCTTGAA
GGTGTTGCAGAAGAAACGGACAACCTGCAAAAGAAGCTCACTAGGATGTTAGCGAGTACC
CAAAAGGTGAATAGGGCAACTGAGAGAATCTTCAAGGAGAGTCAAGACCTGGCCATAGCC
ATTGAGAGGCTGCAGATGAGCATCACAGAAATTATGGAAAAGACAACTTTAAATCAGACT
TTGGATGAAGATTTCCTACTACCCAATTCTACTCTTCAGAACATGCAACAGAATGGTACA
TCTTTGCTAGAAATCATGCAGATAAGAGACTTCACACAGTTGCACCAAAATGCCACCCTT
GAACTCAAGGCTGCTGAAGATTTATTGTCACAAATTCAGGAAAATTACCAGAAGCCGCTG
GAAGAATTGGAGGTATTGAAAGAAGCAGCAAGCCACGTCCTTTCAAAGCACAACAATGAA
CTAAAGGCGGCTGAGGCGCTCGTGAGGGAAGCTGAGGCAAAGATGCAGGAAAGCAACCAC
CTGCTGCTCATGGTCAATGCTAATCTGAGAGAATTCAGTGATAAAAAGCTGCATGTTCAA
GAAGAACAAAATCTGACCTCAGAGCTCATTGTCCAAGGAAGAGGATTGATAGATGCTGCT
GCTGCACAAACAGATGCTGTACAAGATGCTCTAGAGCACTTAGAGGATCACCAGGATAAG
CTACTTTTATGGTCTGCCAAAATCAGGCACCACATAGATGACCTGGTCATGCACATGTCC
CAAAGGAACGCAGTCGACCTGGTCTACAGAGCTGAGGACCATGCCGCTGAGTTCCAGAGA
CTAGCAGATGTTCTGTACAGTGGCCTTGAAAACATCAGAAATGTGTCCCTGAATGCCACC
AGTGCAGCCTATGTCCATTACAACATCCAGAGCCTGATTGAAGAATCGGAGGAACTGGCC
AGAGATGCTCACAGGACTGTGACTGAGACGAGCCTGCTCTCAGAATCCCTTGTTTCTAAC
GGGAAAGCGGCCGTGCAGCGCAGCTCCAGATTTCTAAAAGAAGGCAACAACCTCAGCAGG
AAGCTTCCAGGTATTGCATTGGAACTGAGTGAATTGAGAAATAAGACAAACAGATTTCAA
GAGAATGCTGTTGAAATTACCAGGCAAACCAATGAATCACTCTTGATACTTAGAGCAATT
CCTAAAGGTATAAGAGACAAGGGAGCCAAAACCAAAGAGCTGGCCACGTCTGCAAGCCAG
AGCGCGGTGAGCACGCTGAGGGACGTGGCGGGGCTGAGCCAGGAGCTGCTGAACACATCT
GCCAGCCTGTCCAGGGTCAACACCACATTACGAGAGACACACCAGCTTCTGCAGGACTCC
ACCATGGCCACTCTGTTGGCTGGAAGAAAAGTCAAAGACGTGGAAATTCAAGCCAACCTT
TTGTTTGATCGGTTGAAGCCTTTGAAGATGTTAGAGGAGAATCTGAGCAGAAACCTATCA
GAAATTAAACTGTTGATCAGCCAGGCCCGCAAACAAGCAGCTTCTATTAAAGTCGCCGTG
TCTGCAGACAGAGATTGCATCCGGGCCTACCAGCCTCAGATTTCCTCTACCAACTACAAT
ACCTTAACACTAAATGTTAAGACACAGGAACCCGATAATCTTCTCTTCTACCTCGGTAGC
AGCACCGCTTCTGATTTCCTTGCAGTGGAGATGCGGCGAGGGAGAGTGGCCTTCCTGTGG
GACCTGGGCTCCGGGTCCACACGCTTGGAGTTTCCAGACTTTCCCATTGATGACAACAGA
TGGCACAGTATCCATGTAGCCAGATTTGGAAACATTGGTTCACTGAGTGTAAAGGAAATG
AGCTCAAATCAAAAGTCACCAACAAAAACAAGTAAATCCCCTGGGACAGCTAATGTTCTG
GATGTAAACAATTCAACACTCATGTTTGTTGGAGGTCTTGGAGGACAAATCAAGAAATCT
CCTGCTGTGAAGGTTACTCATTTTAAAGGCTGCTTGGGGGAGGCCTTCCTGAATGGAAAA
TCCATAGGCCTATGGAACTATATTGAAAGGGAAGGCAAGTGCCGTGGGTGCTTCGGAAGC
TCCCAGAATGAAGACCCTTCCTTCCATTTTGACGGGAGTGGGTACTCTGTCGTGGAGAAG
TCACTTCCGGCTACCGTGACCCAGATAATCATGCTTTTTAATACCTTTTCACCTAATGGA
CTTCTTCTCTACCTGGGTTCATACGGCACAAAAGACTTTTTATCCATCGAGCTGTTTCGT
GGCAGAGTGAAGGTTATGACTGACCTGGGTTCAGGACCCATTACCCTTTTGACAGACAGA
CGTTATAACAATGGAACCTGGTACAAAATTGCCTTCCAGCGAAACCGGAAGCAAGGAGTG
CTAGCAGTTATCGATGCCTATAACACCAGTAATAAAGAAACCAAGCAGGGCGAGACTCCG
GGAGCATCTTCTGACCTCAACCGCCTAGACAAGGACCCGATTTATGTGGGTGGATTACCA
AGGTCAAGAGTTGTAAGGAGAGGTGTCACCACCAAAAGCTTTGTGGGCTGCATCAAGAAC
CTGGAAATATCCAGATCAACCTTTGACTTACTCAGAAATTCCTATGGAGTGAGAAAAGGC
TGTTTACTGGAGCCCATCCGGAGTGTTAGCTTCCTGAAAGGCGGCTACATTGAATTGCCA
CCCAAATCTTTGTCACCAGAATCAGAATGGCTGGTAACATTTGCCACCACGAACAGCAGT
GGCATCATCCTGGCTGCCCTCGGCGGGGATGTGGAGAAGCGGGGTGATCGTGAGGAAGCA
CACGTGCCCTTCTTTTCCGTCATGCTGATCGGAGGCAACATTGAGGTACATGTCAATCCT
GGGGATGGGACAGGCCTGAGAAAAGCTCTCCTGCACGCTCCCACGGGTACCTGCAGTGAT
GGACAAGCGCATTCCATCTCCTTGGTCAGGAATCGGAGAATTATCACTGTCCAATTGGAT
GAGAACAATCCTGTGGAAATGAAGTTGGGCACATTAGTAGAAAGCAGGACGATAAATGTG
TCCAATCTGTACGTCGGGGGAATTCCAGAGGGAGAGGGGACGTCACTGCTCACAATGAGA
AGATCGTTCCATGGCTGTATCAAAAACCTGATCTTCAATTTGGAACTTTTGGATTTCAAC
AGTGCAGTTGGCCATGAGCAAGTCGACCTGGACACCTGCTGGCTGTCAGAAAGGCCTAAG
CTGGCTCCCGATGCAGAGGACAGCAAGCTCTTGCCAGAGCCCCGGGCTTTTCCAGAACAG
TGTGTGGTGGATGCAGCTCTGGAGTACGTTCCCGGCGCTCACCAGTTTGGTCTCACACAA
AACAGCCATTTCATCTTGCCTTTTAATCAGTCGGCTGTCAGAAAGAAGCTCTCGGTTGAG
CTAAGCATCCGCACGTTCGCCTCCAGCGGCCTGATTTACTACATGGCTCATCAGAACCAA
GCAGACTACGCTGTGCTCCAGCTGCACGGGGGCCGCCTCCACTTCATGTTTGACCTTGGC
AAAGGCAGAACAAAGGTCTCTCACCCTGCACTGCTCAGTGATGGCAAGTGGCACACGGTC
AAGACAGACTATGTTAAAAGAAAAGGCTTCATAACTGTCGACGGCCGAGAGTCTCCCATG
GTGACTGTGGTGGGAGATGGAACCATGCTGGATGTGGAGGGTTTGTTCTACCTAGGAGGC
CTGCCCTCCCAGTACCAGGCCAGGAAAATTGGAAATATCACCCACAGCATCCCTGCCTGC
ATTGGGGATGTGACGGTTAACAGCAAACAGCTGGACAAGGACAGCCCGGTGTCTGCCTTC
ACGGTGAACAGGTGCTACGCAGTGGCCCAGGAAGGAACATACTTTGACGGAAGCGGATAT
GCAGCTCTTGTCAAAGAGGGCTACAAAGTCCAGTCAGATGTGAACATCACACTGGAGTTT
CGAACCTCCTCGCAGAATGGCGTCCTCCTGGGGATCAGCACTGCCAAAGTGGATGCCATT
GGACTAGAGCTTGTGGACGGCAAGGTCTTGTTCCATGTCAACAATGGTGCTGGCAGGATA
ACAGCTGCATATGAGCCCAAAACCGCCACTGTGCTCTGTGATGGAAAATGGCACACTCTT
CAAGCTAACAAAAGCAAACACCGTATCACTCTGATTGTTGACGGGAACGCAGTTGGCGCT
GAAAGTCCACACACCCAGTCTACCTCAGTGGACACCAACAATCCCATTTATGTTGGTGGC
TATCCTGCTGGTGTGAAGCAAAAATGCCTGCGCAGCCAGACCTCGTTCCGCGGGTGTTTG
AGGAAGCTAGCTCTGATTAAGAGCCCGCAGGTGCAGTCCTTTGACTTCAGCAGAGCGTTC
GAACTGCACGGAGTTTTCCTTCATTCCTGTCCTGGGACCGAGTCCTTG
Protein sequence predicted based on nucleotide sequence
MRGGVLLVLLLCVAAQCRQRGLFPAILNLASNAHISTNATCGEKGPEMFCKLVEHVPGRP VRNPQCRICDGNSANPRERHPISHAIDGTNNWWQSPSIQNGREYHWVTITLDLRQVFQVA YVIIKAANAPRPGNWILERSLDGTTFSPWQYYAVSDSECLSRYNITPRRGPPTYRADDEV ICTSYYSRLVPLEHGEIHTSLINGRPSADDLSPKLLEFTSARYIRLRLQRIRTLNADLMT LSHREPKELDPIVTRRYYYSIKDISVGGMCICYGHASSCPWDETTKKLQCQCEHNTCGES CNRCCPGYHQQPWRPGTVSSGNTCEACNCHNKAKDCYYDESVAKQKKSLNTAGQFRGGGV CINCLQNTMGINCETCIDGYYRPHKVSPYEDEPCRPCNCDPVGSLSSVCIKDDLHSDLHN GKQPGQCPCKEGYTGEKCDRCQLGYKDYPTCVSCGCNPVGSASDEPCTGPCVCKENVEGK ACDRCKPGFYNLKEKNPRGCSECFCFGVSDVCSSLSWPVGQVNSMSGWLVTDLISPRKIP SQQDALGGRHQVSINNTAVMQRLAPKYYWAAPEAYLGNKLTAFGGFLKYTVSYDIPVETV DSNLMSHADVIIKGNGLTLSTQAEGLSLQPYEEYLNVVRLVPENFQDFHSKRQIDRDQLM TVLANVTHLLIRANYNSAKMALYRLESVSLDIASSNAIDLVVAADVEHCECPQGYTGTSC ESCLSGYYRVDGILFGGICQPCECHGHAAECNVHGVCIACAHNTTGVHCEQCLPGFYGEP SRGTPGDCQPCACPLTIASNNFSPTCHLNDGDEVVCDWCAPGYSGAWCERCADGYYGNPT VPGESCVPCDCSGNVDPSEAGHCDSVTGECLKCLGNTDGAHCERCADGFYGDAVTAKNCR ACECHVKGSHSAVCHLETGLCDCKPNVTGQQCDQCLHGYYGLDSGHGCRPCNCSVAGSVS DGCTDEGQCHCVPGVAGKRCDRCAHGFYAYQDGSCTPCDCPHTQNTCDPETGECVCPPHT QGVKCEECEDGHWGYDAEVGCQACNCSLVGSTHHRCDVVTGHCQCKSKFGGRACDQCSLG YRDFPDCVPCDCDLRGTSGDACNLEQGLCGCVEETGACPCKENVFGPQCNECREGTFALR ADNPLGCSPCFCSGLSHLCSELEDYVRTPVTLGSDQPLLRVVSQSNLRGTTEGVYYQAPD FLLDAATVRQHIRAEPFYWRLPQQFQGDQLMAYGGKLKYSVAFYSLDGVGTSNFEPQVLI KGGRIRKQVIYMDAPAPENGVRQEQEVAMRENFWKYFNSVSEKPVTREDFMSVLSDIEYI LIKASYGQGLQQSRISDISMEVGRKAEKLHPEEEVASLLENCVCPPGTVGFSCQDCAPGY HRGKLPAGSDRGPRPLVAPCVPCSCNNHSDTCDPNTGKCLNCGDNTAGDHCDVCTSGYYG KVTGSASDCALCACPHSPPASFSPTCVLEGDHDFRCDACLLGYEGKHCERCSSSYYGNPQ TPGGSCQKCDCNPHGSVHGDCDRTSGQCVCRLGASGLRCDECEPRHILMETDCVSCDDEC VGVLLNDLDEIGDAVLSLNLTGIIPVPYGILSNLENTTKYLQESLLKENMQKDLGKIKLE GVAEETDNLQKKLTRMLASTQKVNRATERIFKESQDLAIAIERLQMSITEIMEKTTLNQT LDEDFLLPNSTLQNMQQNGTSLLEIMQIRDFTQLHQNATLELKAAEDLLSQIQENYQKPL EELEVLKEAASHVLSKHNNELKAAEALVREAEAKMQESNHLLLMVNANLREFSDKKLHVQ EEQNLTSELIVQGRGLIDAAAAQTDAVQDALEHLEDHQDKLLLWSAKIRHHIDDLVMHMS QRNAVDLVYRAEDHAAEFQRLADVLYSGLENIRNVSLNATSAAYVHYNIQSLIEESEELA RDAHRTVTETSLLSESLVSNGKAAVQRSSRFLKEGNNLSRKLPGIALELSELRNKTNRFQ ENAVEITRQTNESLLILRAIPKGIRDKGAKTKELATSASQSAVSTLRDVAGLSQELLNTS ASLSRVNTTLRETHQLLQDSTMATLLAGRKVKDVEIQANLLFDRLKPLKMLEENLSRNLS EIKLLISQARKQAASIKVAVSADRDCIRAYQPQISSTNYNTLTLNVKTQEPDNLLFYLGS STASDFLAVEMRRGRVAFLWDLGSGSTRLEFPDFPIDDNRWHSIHVARFGNIGSLSVKEM SSNQKSPTKTSKSPGTANVLDVNNSTLMFVGGLGGQIKKSPAVKVTHFKGCLGEAFLNGK SIGLWNYIEREGKCRGCFGSSQNEDPSFHFDGSGYSVVEKSLPATVTQIIMLFNTFSPNG LLLYLGSYGTKDFLSIELFRGRVKVMTDLGSGPITLLTDRRYNNGTWYKIAFQRNRKQGV LAVIDAYNTSNKETKQGETPGASSDLNRLDKDPIYVGGLPRSRVVRRGVTTKSFVGCIKN LEISRSTFDLLRNSYGVRKGCLLEPIRSVSFLKGGYIELPPKSLSPESEWLVTFATTNSS GIILAALGGDVEKRGDREEAHVPFFSVMLIGGNIEVHVNPGDGTGLRKALLHAPTGTCSD GQAHSISLVRNRRIITVQLDENNPVEMKLGTLVESRTINVSNLYVGGIPEGEGTSLLTMR RSFHGCIKNLIFNLELLDFNSAVGHEQVDLDTCWLSERPKLAPDAEDSKLLPEPRAFPEQ CVVDAALEYVPGAHQFGLTQNSHFILPFNQSAVRKKLSVELSIRTFASSGLIYYMAHQNQ ADYAVLQLHGGRLHFMFDLGKGRTKVSHPALLSDGKWHTVKTDYVKRKGFITVDGRESPM VTVVGDGTMLDVEGLFYLGGLPSQYQARKIGNITHSIPACIGDVTVNSKQLDKDSPVSAF TVNRCYAVAQEGTYFDGSGYAALVKEGYKVQSDVNITLEFRTSSQNGVLLGISTAKVDAI GLELVDGKVLFHVNNGAGRITAAYEPKTATVLCDGKWHTLQANKSKHRITLIVDGNAVGA ESPHTQSTSVDTNNPIYVGGYPAGVKQKCLRSQTSFRGCLRKLALIKSPQVQSFDFSRAF ELHGVFLHSCPGTESL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
