Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00879148
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5106nts
Open reading frame: 1 to 5106
ATGGGCAACTCCGACAGTCAGTACACCCTTCAAGGATCTAAAAATCATAGCAATACTATT
ACTGGTGCTAAGCAAATTCCTTGCTCCCTGAAAATACGTGGCATTCATGCAAAAGAGGAA
AAGTCATTGCATGGATGGGGTCACGGAAGCAACGGAGCAGGTTACAAGTCCAGGTCCCTG
GCCCGAAGCTGCCTTTCTCACTTTAAGAGTAACCAGCCTTACGCATCGAGACTCGGTGGC
CCCACATGCAAGGTCTCCAGAGGTGTTGCCTACTCCACGCACAGGACAAATGCCCCAGGG
AAGGATTTCCAGGGCATCAGTGCTGCTTTCTCAACTGAGAATGGCTTCCACTCTGTTGGC
CACGAGCTGGCAGATAACCACATCACCTCCAGAGACTGCAACGGACACCTTCTCAACTGC
TACGGGAGGAATGAGAGCATTGCCTCCACCCCACCGGGCGAAGACCGCAAGAGCCCCCGA
GTGCTCATCAAAACGCTGGGGAAGCTGGATGGGTGTTTAAGGGTCGAGTTCCACAATGGT
GGCAACCCCAGCAAAGTGCCTGCAGAGGACTGCAGTGAGCCGGTGCAGCTGCTGAGGTAC
TCACCTACCTTAGCATCGGAAACCTCCCCTGTGCCTGAAGCCAGGAGGGGGTCCAGCGCC
GATTCCCTGCCCAGCCATCGCCCCTCTCCCACGGACTCTCGCCTGCGGTCCAGCAAAGGC
AGCTCCCTGAGTTCTGAGTCATCCTGGTACGACTCCCCTTGGGGCAATGCTGGAGAGCTG
AGCGAGGCTGAGGGCTCCTTCCTGGCCCCCGGCATGCCTGACCCCAGTCTCCATGCCAGC
TTCCCACCTGGCGATGCCAAAAAGCCTTTCAACCAAAGCTCTTCCCTCTCCTCCCTCCGG
GAACTGTACAAAGATGCCAACCTGGGGAGCCTCTCCCCCTCAGGTATCCGCCTTTCTGAT
GAATACATGGGCACGCATGCCAGCCTGAGCAACCGTGTCTCTTTTGCTTCCGACATTGAT
GTGCCCTCCAGAGTGGCACACGGGGACCCCATCCAGTACAGTTCCTTCACTCTCCCCTGT
CGGAAGCCCAAAGCCTTTGTTGAGGATACTGCGAAGAAGGACTCCCTCAAAGCCAGGATG
CGACGGATCAGTGACTGGACGGGAAGCCTCTCAAGGAAGAAAAGGAAACTCCAGGAGCCG
AGGTCCAAGGAGGGCAGTGACTACTTTGACAGTCGCTCTGATGGACTGAATACAGATGTG
CAGGGATCCTCCCAGGCATCTGCTTTTCTGTGGTCAGGGGGCTCTACTCAGATCCTGTCT
CAGAGAAGTGAATCCACACATGCGATTGGCAGCGATCCCCTCCGGCAGAACATTTATGAG
AATTTCATGCGAGAGTTGGAAATGAGCAGGACCAACACTGAGAACATAGAAACATCTACA
GAAACCGCCGAGTCCAGCAGCGAGTCACTCAGCTCTCTGGAACAGCTGGATCTGCTCTTT
GAGAAGGAACAGGGGGTGGTCCGGAAGGCCGGGTGGCTCTTCTTCAAGCCCCTGGTCACT
GTGCAGAAGGAAAGGAAGCTTGAGCTGGTGGCACGAAGGAAATGGAAACAGTACTGGGTA
ACGCTGAAAGGATGCACGCTGCTGTTTTATGAGACCTATGGGAAGAATTCCATGGATCAG
AGCAGTGCCCCTCGGTGTGCTCTGTTTGCAGAAGACAGCATAGTGCAGTCTGTTCCAGAG
CATCCCAAGAAAGAAAATGTGTTCTGCCTCAGCAACTCCTTTGGAGATGTCTACCTTTTC
CAGGCCACCAGCCAGACAGATCTAGAAAACTGGGTCACTGCTGTACACTCTGCTTGTGCA
TCCCTTTTTGCAAAGAAGCATGGGAAAGAGGACACGCTGCGGCTGCTGAAGAACCAGACC
AAAAACCTGCTTCAGAAGATAGACATGGACAGCAAGATGAAGAAGATGGCAGAGCTGCAG
CTGTCCGTGGTGAGCGACCCAAAGAACAGGAAAGCCATAGAGAACCAGATCCAGCAATGG
GAGCAGAATCTTGAGAAATTTCACATGGATCTGTTCAGGATGCGCTGCTATCTGGCCAGC
CTACAAGGTGGGGAGTTACCGAACCCAAAGAGTCTCCTTGCAGCCGCCAGCCGCCCCTCC
AAGCTGGCCCTCGGCAGGCTGGGCATCTTGTCTGTTTCCTCTTTCCATGCTCTGGTATGT
TCTAGAGATGACTCTGCTCTCCGGAAAAGGACACTGTCACTGACCCAGCGAGGGAGAAAC
AAGAAGGGAATATTTTCTTCGTTAAAAGGGCTGGACACACTGGCCAGAAAAGGCAAGGAG
AAGAGACCTTCTATAACTCAGGTCGATGAACTTCTGCATATATATGGTTCAACAGTAGAC
GGTGTTCCCCGAGACAATGCATGGGAAATCCAGACTTATGTCCACTTTCAGGACAATCAC
GGAGTTACTGTAGGGATCAAGCCAGAGCACAGAGTAGAAGATATTTTGACTTTGGCATGC
AAGATGAGGCAGTTGGAACCCAGCCATTATGGCCTACAGCTTCGAAAATTAGTAGATGAC
AATGTTGAGTATTGCATCCCTGCACCATATGAATATATGCAACAACAGGTTTATGATGAA
ATAGAAGTCTTTCCACTAAATGTTTATGACGTGCAGCTCACGAAGACTGGGAGTGTGTGT
GACTTTGGGTTTGCAGTTACAGCGCAGGTGGATGAGCGTCAGCATCTCAGCCGGATATTT
ATAAGCGACGTTCTTCCCGATGGCCTGGCGTATGGGGAAGGGCTGAGAAAGGGCAATGAG
ATCATGACCTTAAATGGGGAAGCTGTGTCTGATCTTGACCTTAAGCAGATGGAGGCCCTG
TTTTCTGAGAAGAGCGTCGGACTCACTCTGATTGCCCGGCCTCCGGACACAAAAGCAACC
CTGTGTACATCCTGGTCAGACAGTGACCTGTTCTCCAGGGACCAGAAGAGTCTGCTGCCC
CCTCCTAACCAGTCCCAACTGCTGGAGGAATTCCTGGATAACTTTAAAAAGAATACAGCC
AATGATTTCAGCAACGTCCCTGATATCACAACAGGTCTGAAAAGGAGTCAGACAGATGGC
ACTCTGGATCAGGTTTCCCACAGGGAGAAAATGGAGCAGACATTCAGGAGTGCTGAGCAG
ATCACTGCACTGTGCAGGAGTTTTAACGACAGTCAGGCCAACGGCATGGAAGGACCGCGG
GAGAATCAGGATCCTCCTCCGAGGCCTCTGGCCCGCCACCTGTCTGATGCAGACCGCCTC
CGCAAAGTCATCCAGGAGCTTGTGGACACAGAGAAGTCCTACGTGAAGGATTTGAGCTGC
CTCTTTGAATTATACTTGGAGCCACTTCAGAATGAGACCTTTCTTACCCAAGATGAGATG
GAGTCACTTTTTGGAAGTTTGCCAGAGATGCTTGAGTTTCAGAAGGTGTTTCTGGAGACC
CTGGAGGATGGGATTTCAGCATCATCTGACTTTAACACCCTAGAAACCCCCTCACAGTTT
AGAAAATTACTGTTTTCCCTTGGAGGCTCTTTCCTTTATTACGCGGACCACTTTAAACTG
TACAGTGGATTCTGTGCTAACCATATCAAAGTACAGAAGGTTCTGGAGCGAGCTAAAACT
GACAAAGCCTTCAAGGCTTTTCTGGACGCCCGGAACCCCACCAAGCAGCATTCCTCCACG
CTGGAGTCCTACCTCATCAAGCCGGTTCAGAGAGTGCTCAAGTACCCGCTGCTGCTCAAG
GAGCTGGTGTCCCTGACGGACCAGGAGAGCGAGGAGCACTACCACCTGACGGAAGCACTA
AAGGCAATGGAGAAAGTAGCGAGCCACATCAATGAGATGCAGAAGATCTATGAGGATTAT
GGGACCGTGTTTGACCAGCTAGTAGCTGAGCAGAGCGGAACAGAGAAGGAGGTAACAGAA
CTTTCGATGGGAGAGCTTCTGATGCACTCTACGGTTTCCTGGTTGAATCCATTTCTGTCT
CTAGGAAAAGCTAGAAAGGACCTTGAGCTCACAGTATTTGTTTTTAAGAGAGCCGTCATA
CTGGTTTATAAAGAAAACTGCAAACTGAAAAAGAAATTGCCCTCGAATTCCCGGCCTGCA
CACAACTCTACTGACTTGGACCCATTTAAATTCCGCTGGTTGATCCCCATCTCCGCGCTT
CAAGTCAGACTGGGGAATCCAGCAGGGACAGAAAATAATTCCATATGGGAACTGATCCAT
ACGAAGTCAGAAATAGAAGGACGGCCAGAAACCATCTTTCAGTTGTGTTGCAGTGACAGT
GAAAGCAAAACCAACATTGTTAAGGTGATTCGTTCTATTCTGAGGGAGAACTTCAGGCGT
CACATAAAGTGTGAATTACCACTGGAGAAAACGTGTAAGGATCGCCTGGTACCTCTTAAG
AACCGAGTTCCTGTTTCGGCCAAATTAGCTTCATCCAGGTCTTTAAAAGTCCTGAAGAAT
TCCTCCAGCAACGAGTGGACCGGTGAGACTGGCAAGGGAACCTTGCTGGACTCTGACGAG
GGCAGCTTGAGCAGCGGCACCCAGAGCAGCGGCTGCCCCACGGCTGAGGGCAGGCAGGAC
TCCAAGAGCACTTCTCCCGGGAAATACCCACACCCCGGCTTGGCAGATTTTGCCGACAAT
CTCATCAAAGAGAGTGACATCCTGAGCGATGAAGATGATGACCACCGTCAGACTGTGAAG
CAGGGCAGCCCTACTAAAGACATCGAAATTCAGTTCCAGAGACTGAGGATTTCCGAGGAC
CCAGACGTTCACCCCGAGGCTGAGCAGCAGCCTGGCCCGGAGTCGGGTGAGGGTCAGAAA
GGAGGAGAGCAGCCCAAACTGGTCCGGGGGCACTTCTGCCCCATTAAACGAAAAGCCAAC
AGCACCAAGAGGGACAGAGGAACTTTGCTCAAGGCGCAGATCCGTCACCAGTCCCTTGAC
AGTCAGTCTGAAAATGCCACCATCGACCTAAATTCTGTTCTAGAGCGAGAATTCAGTGTC
CAGAGTTTAACATCTGTTGTCAGTGAGGAGTGTTTTTATGAAACAGAGAGCCACGGAAAA
TCATTG
Protein sequence predicted based on nucleotide sequence
MGNSDSQYTLQGSKNHSNTITGAKQIPCSLKIRGIHAKEEKSLHGWGHGSNGAGYKSRSL ARSCLSHFKSNQPYASRLGGPTCKVSRGVAYSTHRTNAPGKDFQGISAAFSTENGFHSVG HELADNHITSRDCNGHLLNCYGRNESIASTPPGEDRKSPRVLIKTLGKLDGCLRVEFHNG GNPSKVPAEDCSEPVQLLRYSPTLASETSPVPEARRGSSADSLPSHRPSPTDSRLRSSKG SSLSSESSWYDSPWGNAGELSEAEGSFLAPGMPDPSLHASFPPGDAKKPFNQSSSLSSLR ELYKDANLGSLSPSGIRLSDEYMGTHASLSNRVSFASDIDVPSRVAHGDPIQYSSFTLPC RKPKAFVEDTAKKDSLKARMRRISDWTGSLSRKKRKLQEPRSKEGSDYFDSRSDGLNTDV QGSSQASAFLWSGGSTQILSQRSESTHAIGSDPLRQNIYENFMRELEMSRTNTENIETST ETAESSSESLSSLEQLDLLFEKEQGVVRKAGWLFFKPLVTVQKERKLELVARRKWKQYWV TLKGCTLLFYETYGKNSMDQSSAPRCALFAEDSIVQSVPEHPKKENVFCLSNSFGDVYLF QATSQTDLENWVTAVHSACASLFAKKHGKEDTLRLLKNQTKNLLQKIDMDSKMKKMAELQ LSVVSDPKNRKAIENQIQQWEQNLEKFHMDLFRMRCYLASLQGGELPNPKSLLAAASRPS KLALGRLGILSVSSFHALVCSRDDSALRKRTLSLTQRGRNKKGIFSSLKGLDTLARKGKE KRPSITQVDELLHIYGSTVDGVPRDNAWEIQTYVHFQDNHGVTVGIKPEHRVEDILTLAC KMRQLEPSHYGLQLRKLVDDNVEYCIPAPYEYMQQQVYDEIEVFPLNVYDVQLTKTGSVC DFGFAVTAQVDERQHLSRIFISDVLPDGLAYGEGLRKGNEIMTLNGEAVSDLDLKQMEAL FSEKSVGLTLIARPPDTKATLCTSWSDSDLFSRDQKSLLPPPNQSQLLEEFLDNFKKNTA NDFSNVPDITTGLKRSQTDGTLDQVSHREKMEQTFRSAEQITALCRSFNDSQANGMEGPR ENQDPPPRPLARHLSDADRLRKVIQELVDTEKSYVKDLSCLFELYLEPLQNETFLTQDEM ESLFGSLPEMLEFQKVFLETLEDGISASSDFNTLETPSQFRKLLFSLGGSFLYYADHFKL YSGFCANHIKVQKVLERAKTDKAFKAFLDARNPTKQHSSTLESYLIKPVQRVLKYPLLLK ELVSLTDQESEEHYHLTEALKAMEKVASHINEMQKIYEDYGTVFDQLVAEQSGTEKEVTE LSMGELLMHSTVSWLNPFLSLGKARKDLELTVFVFKRAVILVYKENCKLKKKLPSNSRPA HNSTDLDPFKFRWLIPISALQVRLGNPAGTENNSIWELIHTKSEIEGRPETIFQLCCSDS ESKTNIVKVIRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKLASSRSLKVLKN SSSNEWTGETGKGTLLDSDEGSLSSGTQSSGCPTAEGRQDSKSTSPGKYPHPGLADFADN LIKESDILSDEDDDHRQTVKQGSPTKDIEIQFQRLRISEDPDVHPEAEQQPGPESGEGQK GGEQPKLVRGHFCPIKRKANSTKRDRGTLLKAQIRHQSLDSQSENATIDLNSVLEREFSV QSLTSVVSEECFYETESHGKSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
