Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00879170
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4995nts
Open reading frame: 1 to 4995
ATGGCGGCGCGCCGGGGACGGAGAGACGGAGTCGCGCCGCCCCCGAGTGGGGGCCCCGGT
CCGGACCCTGGCGGGGGAGCCCGCGGCAGTGGTTGGGGAAGTCGAAGCCAAGCGCCGTAT
GGGACTTTGGGCGCTGTGAGCGGCGGCGAGCAGGTGCTGCTGCATGAGGAGGCGGGTGAT
TCTGGCTTTGTCAGTCTCTCTCGGCTGGGCCCATCTCTGAGGGACAAGGACCTGGAAATG
GAGGAGCTAATGCTGCAGGATGAGACACTGCTGGGGACCATGCAGAGCTACATGGATGCC
TCCCTTATCTCCCTCATTGAGGATTTTGGGAGCCTTGGAGAGAGCAGGTTATCTCTGGAG
GACCAGAATGAAGTGTCGCTGCTCACGGCTCTGACGGAGATCTTGGACAATGCAGATTCT
GAGAACCTTTCTCCATTTGACAGCATTCCTGATTCGGAGCTGCTTGTGTCACCCCGGGAG
GGCTCCTCTCTGCACAAGCTGCTTACTCTCTCTCGGACACCCCCAGAACGTGACCTCATC
ACCCCAGTTGACCCACTGGGGCCCAGTACAGGCAGCAGTAGAGGGAGTGGGGTTGAAATG
TCTCTTCCAGATCCCTCTTGGGACTTCTCCCCACCCTCTTTCTTAGAGACCTCTTCCCCC
AAGCTTCCTAGCTGGAGACCCCCAAGATCAAGACCACGCTGGGGCCAATCCCCACCTCCC
CAGCAGCGCAGTGATGGAGAAGAAGAGGAGGAGGTGGCCAGCTTCAGTGGCCAGATTCTT
GCCGGGGAGCTTGACAACTGTGTGAGCAGTATCCCGGACTTCCCCATGCATTTGGCCTGC
CCTGAGGAGGAAGATAAAGCAACAGCAGCAGAGATGGCAGTGCCAGCAGCTGGTGATGAG
AGCATCTCCTCCCTGAGTGAGCTGGTGCGGGCCATGCACCCATACTGCCTGCCCAACCTC
ACCCACCTGGCATCACTTGAGGATGAGCTTCAGGAGCAGCCAGATGATTTGACACTGCCT
GAGGGCTGCGTAGTGCTGGAGATTGTGGGGCAGGCAGCCACAGCTGGCGATGACCTGGAG
ATCCCAGTTGTGGTGCGACAGGTCTCTCCTGGACCCCGGCCTGTGCTCCTGGATGACTCG
CTAGAGACTAGTTCTGCCTTGCAGCTGCTTATGCCTACACTGGAGTCAGAGACAGAGGCT
GCTGTGCCCAAGGTAACCCTCTGCTCTGAGAAAGAGGGGTTGTCATTGAACTCAGAGGAG
AAGCTGGACTCAGCCTGCTTATTGAAGCCCAGGGAGGTCGTGGAGCCGGTGGTGCCCAAG
GAGCCTCAGAACCCACCTGCCAATGCAGCACCAGGTTCCCAGAGAGCTCGAAAGGGCAGG
AAGAAGAAGAGCAAGGAGCAGCCAGCAGCCTGTGTGGAAGGCTATGCCAGGAGGCTGAGG
TCATCTTCTCGCGGGCAGTCTACTGTAGGTACAGAAGTGACCTCTCAGGTAGACAACTTG
CAGAAACAGCCTCAGGAAGAACTTCAAAAAGAGTCTGGGCCTCTCCAGGGTAAGGGGAAG
CCCCGGGCTTGGGCTCGGGCCTGGGCAGCTGCCTTGGAGAATTCTAGCCCTAAGAACTTG
GAGAGAAGTGCTGGACAAAGTAGTCCTGCTAAAGAAGGCCCTCTAGACCTCTACCCAAAG
CTGGCTGACACTATCCAAACCAATCCTATACCAACCCATCTCTCATTGGTCGACTCTGCC
CAAGCCAGCCCCATGCCAGTTGACTCTGTTGAAGCTGATCCCACTGCAGTTGGCCCTGTT
CTAGCTGGCCCTGTACCTGTTGACCCTGGGTTGGTTGACCTTGCTTCAACCAGCTCAGAA
CTGGTTGAGCCTCTCCCGGCTGAGCCAGTGCTGATCAACCCAGTCCTGGCTGACTCAGCA
GCAGTTGACCCTGCAGTGGTTCCCATCTCAGATAACTTGCCACCAGTTGATGCTGTCCCG
TCTGGCCCAGCACCAGTTGATCTAGCACTGGTTGACCCTGTTCCTAATGACCTGACTCCA
GTTGACCCAGTGCTAGTTAAGTCCAGACCAACTGATCCCAGACGTGGTGCAGTGTCATCA
GCCCTGGGGGGTTCAGCACCCCAGCTCCTCGTGGAGTCAGAGTCCTTGGACCCACCAAAG
ACCATCATCCCTGAAGTCAAAGAGGTTGTGGATTCTCTGAAAATTGAAAGTGGTACCAGT
GCTACAACCCATGAAGCCAGACCTCGGCCTCTCAGCTTATCTGAGTACCGGCGACGAAGG
CAGCAACGCCAAGCAGAAACAGAAGAGAGAAGTCCACAGCCCCCAACTGGGAAGTGGCCT
AGCCTTCCAGAGACTCCCACAGGGCTGGCAGACATCCCTTGTCTTGTCATCCCACCAGCC
CCAGCCAAGAAGACAGCTCTGCAGAGAAGCCCTGAAACACCCCTTGAGATTTGCCTTGTG
CCTGTAGGTCCCAGCCCTGCTTCTCCTAGTCCTGAGCCACCTGTAAGCAAACCTGTGGCC
TCATCTCCCACTGAGCAGGTGCCATCCCAGGAGATGCCACTGTTGGCGAGACCTTCCCCT
CCTGTGCAGTCTGTGTCCCCTGCTGTGCCCACACCTCCCTCGATGTCTGCTGCCCTGCCT
TTCCCTGCAGGTGGGCTTGGCATGCCCCCCAGTCTGCCCCCACCTCCCTTGCAGCCTCCT
AGTCTTCCATTGTCTATGGGGCCAGTACTACCTGATCCGTTTACTCACTATGCCCCCTTG
CCATCCTGGCCTTGTTATCCTCATGTGTCCCCTTCTGGCTATCCTTGCCTGCCCCCCCCA
CCAACGGTGCCCCTAGTGTCTGGTACTCCTGGTGCCTATGCCGTGCCTCCCACTTGCAGT
GTGCCTTGGGCACCCCCTCCTGCCCCAGTCTCACCTTACAGTTCCACATGTACCTATGGG
CCCTTGGGATGGGGCCCAGGGCCTCAACATGCTCCATTCTGGTCTACTGTTCCCCCACCT
CCTTTGCCTCCAGCCTCCATTGGGAGAGCTGTTCCCCAACCTAAAATGGAGTCTAGGGGC
ACTCCAGCTGGCCCTCCTGAAAATGTACTTCCCTTGTCGATGGCTCCTCCCCTCAGTCTT
GGGCTACCTGGCCATGGAGCTCCTCAGACAGAGCCTACCAAGGTGGAGGTCAAGCCAGTG
CCTGCATCTCCCCATCCGAAACACAAGGTGTCTGCCCTGGTGCAAAGTCCCCAGATGAAG
GCTCTAGCATGTGTGTCTGCTGAAGGTGTGACTGTTGAGGAGCCTGCATCAGAGAGGCTA
AAGCCTGAGACCCAAGAGACCAGGCCCAGGGAGAAGCCCCCCTTGCCTGCTACCAAGGCT
GTTCCCACACCAAGGCAGAGCACTGTCCCCAAGCTGCCTGCTGTCCACCCAGCCCGTCTA
AGGAAGCTGTCCTTCCTGCCTACCCCACGTACTCAGGGTTCTGAAGATGTGGTACAGGCT
TTCATCAGTGAGATTGGAATTGAGGCATCGGACCTGTCCAGTCTGCTGGAGCAGTTTGAG
AAATCAGAAGCCAAAAAGGAGTGTCCTCCTCCGGCTCCTGCTGACAGCTTGGCTGTAGGA
AACTCAGGCGGCGTTGACATTCCCCAGGAGAAGAGGCCCCTAGACCGGTTACAAGCCCCA
GAACTGGCCAACGTGGCAGGGCTCACCCCTCCAGCTACCCCTCCCCACCAGTTATGGAAG
CCCCTGGCTGCTGTCTCACTGCTGGCCAAAGCCAAATCTCCTAAGTCCACCGCCCAGGAG
GGAACCCTGAAGCCTGAAGGAGTTACGGAGGCCAAACATCCAGCTGCAGTTCGCCTCCAA
GAAGGGGTCCATGGCCCTAGTCGAGTCCATGTGGGCTCTGGGGACCATGACTATTGTGTC
CGGAGCAGGACCCCCCCAAAAAAGATGCCTGCCCTAGTCATTCCAGAGGTGGGCTCCCGA
TGGAATGTCAAGCGCCATCAGGACATCACCATCAAACCTGTCTTGTCCTTGGGCCCAGCT
GCCCCTCCGCCCCCATGCATAGCTGCCTCCCGGGAGCCGCTTGATCACAGGACTAGCAGT
GAGCAGGCAGATCCCTCAGCACCCTGCCTTGCCCCATCCAGCTTGCTGTCCCCTGAGGCC
TCACCCTGCCGGAATGACATGAACACTAGGACTCCCCCTGAACCCTCAGCCAAGCAGCGG
TCAATGCGCTGTTACCGAAAAGCCTGCAGGTCAGCCAGCCCCTCAAGCCAGGGCTGGCAG
GGCCGCCGAGGCCGCAACAGCCGTTCTGTCAGCTCTGGGTCCAACCGGACTAGCGAAGCA
TCTTCCTCATCCTCATCATCGTCTTCCTCATCCCGATCTCGGTCCAGGTCCCTCTCCCCC
CCACACAAGAGGTGGCGAAGGTCCAGCTGTAGTTCCTCTGGACGTTCTCGAAGATGCTCT
TCCTCTTCTTCGTCATCATCTTCCTCTTCGTCTTCCTCATCCTCATCATCCAGTTCTCGA
AGCCGCTCACGATCCCCATCCCCCCGCCGGAGAAGTGACAGGAGGCGGCGGTACAGCTCT
TATCGTTCACATGACCATTACCAAAGGCAAAGAGTGCTACAAAAGGAGCGTGCAATAGAA
GAAAGAAGGGTGGTCTTCATTGGAAAGATACCTGGCCGCATGACTCGATCAGAGCTGAAA
CAGAGGTTCTCCGTTTTTGGAGAGATTGAGGAGTGCACCATCCACTTCCGTGTCCAAGGG
GACAACTACGGCTTCGTCACTTATCGCTATGCTGAGGAGGCATTTGCAGCCATTGAGAGT
GGCCACAAGCTGCGGCAGGCAGATGAGCAGCCCTTTGATCTCTGCTTTGGGGGCCGAAGG
CAGTTCTGCAAGAGGAGCTATTCTGATCTTGACTCCAACCGGGAAGACTTTGACCCAGCA
CCTGTAAAGAGCAAATTTGATTCTCTTGACTTTGACACATTGTTGAAACAGGCCCAGAAG
AACCTCAGGAGGTTG
Protein sequence predicted based on nucleotide sequence
MAARRGRRDGVAPPPSGGPGPDPGGGARGSGWGSRSQAPYGTLGAVSGGEQVLLHEEAGD SGFVSLSRLGPSLRDKDLEMEELMLQDETLLGTMQSYMDASLISLIEDFGSLGESRLSLE DQNEVSLLTALTEILDNADSENLSPFDSIPDSELLVSPREGSSLHKLLTLSRTPPERDLI TPVDPLGPSTGSSRGSGVEMSLPDPSWDFSPPSFLETSSPKLPSWRPPRSRPRWGQSPPP QQRSDGEEEEEVASFSGQILAGELDNCVSSIPDFPMHLACPEEEDKATAAEMAVPAAGDE SISSLSELVRAMHPYCLPNLTHLASLEDELQEQPDDLTLPEGCVVLEIVGQAATAGDDLE IPVVVRQVSPGPRPVLLDDSLETSSALQLLMPTLESETEAAVPKVTLCSEKEGLSLNSEE KLDSACLLKPREVVEPVVPKEPQNPPANAAPGSQRARKGRKKKSKEQPAACVEGYARRLR SSSRGQSTVGTEVTSQVDNLQKQPQEELQKESGPLQGKGKPRAWARAWAAALENSSPKNL ERSAGQSSPAKEGPLDLYPKLADTIQTNPIPTHLSLVDSAQASPMPVDSVEADPTAVGPV LAGPVPVDPGLVDLASTSSELVEPLPAEPVLINPVLADSAAVDPAVVPISDNLPPVDAVP SGPAPVDLALVDPVPNDLTPVDPVLVKSRPTDPRRGAVSSALGGSAPQLLVESESLDPPK TIIPEVKEVVDSLKIESGTSATTHEARPRPLSLSEYRRRRQQRQAETEERSPQPPTGKWP SLPETPTGLADIPCLVIPPAPAKKTALQRSPETPLEICLVPVGPSPASPSPEPPVSKPVA SSPTEQVPSQEMPLLARPSPPVQSVSPAVPTPPSMSAALPFPAGGLGMPPSLPPPPLQPP SLPLSMGPVLPDPFTHYAPLPSWPCYPHVSPSGYPCLPPPPTVPLVSGTPGAYAVPPTCS VPWAPPPAPVSPYSSTCTYGPLGWGPGPQHAPFWSTVPPPPLPPASIGRAVPQPKMESRG TPAGPPENVLPLSMAPPLSLGLPGHGAPQTEPTKVEVKPVPASPHPKHKVSALVQSPQMK ALACVSAEGVTVEEPASERLKPETQETRPREKPPLPATKAVPTPRQSTVPKLPAVHPARL RKLSFLPTPRTQGSEDVVQAFISEIGIEASDLSSLLEQFEKSEAKKECPPPAPADSLAVG NSGGVDIPQEKRPLDRLQAPELANVAGLTPPATPPHQLWKPLAAVSLLAKAKSPKSTAQE GTLKPEGVTEAKHPAAVRLQEGVHGPSRVHVGSGDHDYCVRSRTPPKKMPALVIPEVGSR WNVKRHQDITIKPVLSLGPAAPPPPCIAASREPLDHRTSSEQADPSAPCLAPSSLLSPEA SPCRNDMNTRTPPEPSAKQRSMRCYRKACRSASPSSQGWQGRRGRNSRSVSSGSNRTSEA SSSSSSSSSSSRSRSRSLSPPHKRWRRSSCSSSGRSRRCSSSSSSSSSSSSSSSSSSSSR SRSRSPSPRRRSDRRRRYSSYRSHDHYQRQRVLQKERAIEERRVVFIGKIPGRMTRSELK QRFSVFGEIEECTIHFRVQGDNYGFVTYRYAEEAFAAIESGHKLRQADEQPFDLCFGGRR QFCKRSYSDLDSNREDFDPAPVKSKFDSLDFDTLLKQAQKNLRRL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
