Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00879199
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6663nts
Open reading frame: 1 to 6663
ATGATTCGAATTGCAGCCTTAAATGCCAGCTCCACCATTGAGGATGATCATGAAGGAAGC
TTTAAAAGTCACAAAACCCAGACAAAGGAGGCTCAGGAAGCAGAGGCTTTTGCATTGTAC
CACAAGGCCCTTGATCTGCAGAAACATGACCGGTTTGAGGAGTCTGCCAAAGCCTACCAT
GAGCTCTTGGAGGCGAGCCTGCTGCGGGAGGCAGTTTCATCCGGTGATGAGAAAGAGGGG
TTGAAACACCCTGGGCTGATACTGAAATATTCCACTTATAAGAACTTGGCCCAGCTGGCA
GCCCAGCGGGAGGATCTGGAGACAGCCATGGAGTTCTACTTAGAGGCAGTGATGCTGGAC
TCCACAGATGTCAACCTCTGGTATAAGATTGGACATGTGGCCCTGAGGCTCATCCGGATC
CCCCTGGCTCGCCATGCTTTTGAGGAAGGGCTGCGGTGCAATCCTGACCACTGGCCCTGT
TTGGATAACCTAATCACTGTCCTGTACACCCTCAGTGATTACACAACATGTCTGTACTTC
ATCTGCAAAGCTTTGGAGAAGGATTGCCGGTACAGCAAAGGGCTGGTCCTCAAGGAGAAG
ATTTTTGAGGAGCAGCCTTGTCTCCGGAAGGACTCTCTCAGAATGTTCCTCAAATGTGAC
ATGTCGATTCACGATGTTTCGGTGAGTGCAGCTGAGACACAGGCGATTGTAGATGAGGCC
TTGGGGCTGCGAAAAAAGAGGCAAGCGCTGATTGTGCGGGAGAAGGAGCCGGACCTGAAA
CTTGTGCAGCCCATTCCTTTCTTCACCTGGAAGTGCCTCGGAGAGAGCTTGCTGGCCATG
TACAATCATCTCACCACCTGTGAGCCCCCACGTCCCAGCCTTGGCAAAAGGATTGATTTG
TCGGACTACCAGGACCCCAGCCAGCCTCTTGAGTCCTCCATGGTGGTGACGCCAGTTAAC
GTGATCCAGCCAAGCACTGTCAGCACCAACCCAGCTGTGGCTGTCGCCGAGCCTGTGGTC
TCCTACACCTCTGTGGCTACAACCAGCTTCCCACTGCACAGTCCTGGTCTGTTGGAGACA
GGCGCTCCTGTGGGTGATATTTCTGGGGGAGATAAATCCAAGAAAGGGGTAAAACGGAAG
AAGATTTCAGAAGAGAGTGGAGAAACAGCAAAGCGGCGGTCTGCCCGTGTCCGAAACACC
AAGTGCAAAAAAGAAGAGAAAGTAGACTTCCAGGAGCTTCTGATGAAGTTCTTGCCGTCC
AGGTTAAGAAAGCTGGACCCTGAGGAGGAAGATGATTCCTTTAATAACTATGAAGTCCAG
TCAGAAGCCAAACTGGAAAGCTTCCCAAGCATTGGGCCTCAAAGGCTGTCATTTGACTCA
GCCACATTCATGGAATCTGAAAAGCAGGACGTGCATGAGTTCCTGCTGGAGAACCTAACC
AACGGGGGCATCCTGGAGCTGATGATGCGCTACCTGAAAGCCATGGGCCACAAGTTCTTG
GTAAGGTGGCCTCCAGGCTTGGCGGAGGTCGTGCTCAGCGTCTACCACAGCTGGAGGAGG
CACAGCACCAGCCTGCCCAACCCGCTGCTGAGGGACTGCAGCAACAAGCACATCAAGGAC
ATGATGCTGATGTCTCTCTCCTGCATGGAACTCCAGCTGGACCAGTGGCTGCTGACCAAA
GGCAGAAGCTCTGCAGTGTCTCCTCGGAACTGCCCTGCTGGTATGGTGAATGGCAGATTT
GGACCTGACTTCCCAGGGACCCACTGCCTGGGTGACCTCCTACAGCTGTCATTTGCCTCG
TCCCAGCGCGACCTGTTCGAGGATGGTTGGCTGGAGTTTGTGGTCCGTGTTTACTGGCTG
AAGGCTCGCTTCCTGGCGCTGCAGGGAGACATGGAGCAGGCCCTGGAGAACTATGACATC
TGCACAGAAATGCTCCAGAGTTCCACCGCCATCCAGGTGGAGGCAGGGGCTGAACGAAGA
GACATTGTCATCCGGCTGCCCAACCTCCATAATGACTCTGTGGTTTCCCTGGAGGAGATT
GATAAGAACCTGAAGTCGCTGGAGCGGTGCCAGTCCCTGGAGGAGATTCAGCGGCTGTAT
GAAGCAGGCGACTACAAGGCTGTTGTGCATCTGCTCCGCCCCACTTTGTGCACCAGTGGG
TTTGACCGGGCCAAACACCTGGAGTTTATGACTTCCATTCCTGAGAGGCCAGCCCAGCTG
CTTCTTCTGCAGGACTCCTTGCTCCGGCTGAAGGACTATCGGCAGTGTTTTGAGTGTTCC
GATGTGGCTCTGAACGAGGCTGTCCAGCAGATGGTGAACTCAGGTGAGGCTGCCGCCAAG
GAGGAGTGGGTGGCCACAGTGACCCAACTGCTGATGGGCATCGAGCAGGCCCTCTCTGCG
GACAGCAGTGGTAGCATCCTGAAGGTATCATCCTCCACCACTGGCCTTGTGCGGCTCACC
AACAACCTCATCCAGGTCATTGACTGCAGCATGGCTGTGCAGGAGGAGGCCAAGGAGCCC
CACGTCTCTTCAGTGCTACCCTGGATCATTCTACACCGGATCATCTGGCAGGAGGAAGAC
ACCTTCCATTCTCTGTGCCACCAGCAGCAGCTCCAAAACCCAGCGGAGGAAGGGATGTCA
GAGACGCCCATGCTCCCATCCTCCCTCATGCTGCTGAACACAGCCCACGAGTATTTGGGC
AGAAGGTCCTGGTGCTGCAATTCAGATGGGGCTCTGCTGCGATTCTATGTGCGAGTACTC
CAGAAGGAACTGGCTGCATCCACCTCTGAAGACACGCACCCTTACAAGGAGGAGCTGGAG
ACAGCCTTGGAGCAGTGCTTCTACTGCCTGTACAGCTTCCCCAGCAAGAAGAGTAAGGCC
AGGTACCTGGAGGAACACTCGGCCCAGCAGGTGGATCTTATATGGGAGGATGCACTGTTC
ATGTTTGAGTATTTTAAGCCCAAGACCCTTCCTGAATTTGACAGCTATAAGACCAGCACC
GTGTCTGCTGACTTGGCCAACCTACTGAAGAGAATTGCCACCATTGTGCCTCGCACAGAG
AGGCCAGCCCTTAGCCTGGACAAAGTCTCTGCCTACATTGAGGGAACTTCAACTGAGGTA
CCCTGCCTCCCAGAGGGGGCTGACCCCTCCCCTCCAGTGGTGAACGAGCTTTACTACCTC
CTGGCTGATTATCATTTCAAAAACAAGGAGCAGTCCAAGGCCATCAAGTTCTACATGCAT
GACATCTGCATCTGCCCCAATAGGTTTGATTCCTGGGCAGGCATGGCTCTGGCCCGGGCC
AGCCGCATTCAGGACAAGCTGAACTCCAATGAGCTGAAGAGTGATGGGCCCATTTGGAAG
CATGCCACGCCCGTCTTGAACTGCTTCCGTCGGGCCCTGGAGATTGACAGCTCCAACTTG
TCCCTATGGATTGAGTATGGCACCATGTCCTATGCCTTGCACTCATTCGCCTCACGTCAA
TTGAAGCAGTGGAGAGGCGAGCTGCCCCCTGAGCTCGTGCAGCAGATGGAGGGCCGGCGC
GACAGCATGCTAGAGACAGCCAAGCACTGTTTCACATCAGCAGCCCGCTGCGAGGGTGAT
GGTGACGAGGAGGAGTGGCTCATCCACTACATGCTGGGCAAGGTGGCTGAGAAGCAGCAG
CAGCCACCCACCGTTTACTTGCTGCACTACAGGCAGGCTGGCCACTACCTGCACGAGGAG
GCTGCCCGCTACCCCAAGAAGATCCACTACCACAACCCACCTGAGCTGGCCATGGAGGCC
CTGGAGGTGTACTTTCGGCTCCATGCTTCCATCCTGAAGCTCCTGGGGAAGCCCGATTCT
GGGGTTGGTGCAGAGGTCCTGGTCAACTTTATGAAGGAGGCTGCAGAAGGACCCTTTGCC
AGGGGCGAGGAGAAGAACACACCCAAAGCTTCAGAAAAGGAGAAGGCCTGCCTGGTGGAC
GAGGACTCCCACTCTTCAGCTGGGACACTGCCGGGCCCCGGAGCCTCCCTCCCCTCCTCC
TCTGGCCCAGGTCTGACATCCCCACCTTACACAGCCACTCCGATTGACCACGATTACGTC
AAATGTAAAAAACCCCACCAGCAGGCAACGCCGGACGACCGAAGCCAGGACAGCACAGCC
GTAGCACTCTCAGACTCTAGCTCAACGCAGGACTTCTTTAATGAGCCCACCAGCTTACTG
GAAGGCTCCAGGAAATCCTACACAGAGAAGAGGCTGCCCATTCTCAGTTCCCAAGCAGGA
GCGACGGGTAAAGATCTTCAGGGGGCCACAGAAGAAAGAGGAAAAAACGAGGAGTCATTG
GAGAGTACAGAAGGCTTCCGGGCTGCAGAGCAAGGTGTCCAGAAGCCTGCTGCAGAAACC
CCAGCCTCTGCTTGCATCCCTGGCAAGCCCTCAGCATCCACACCCACCCTGTGGGATGGG
AAGAAGAGAGGGGACCTCCCAGGGGAGCCAGTGGCCTTCCCCCAGGGGCTGCCGGCTGGT
GCTGAGGAGCAGCGGCAGTTTCTCACAGAGCAGTGCATCGCCTCCTTCCGCCTGTGCCTG
AGCCGCTTCCCCCAGCACTATAAGAGTCTCTACCGTCTGGCCTTCCTCTACACCTACAGC
AAGACCCACCGGAACCTCCAGTGGGCCCGCGACGTGTTGCTAGGCAGCAGTATCCCGTGG
CAACAACTGCAGCACATGCCGGCACAGGGGCTCTTCTGCGAGAGGAACAAGACCAATTTC
TTCAACGGCATCTGGCGGATCCCCGTGGACGAGATTGACCGGCCGGGCAGCTTTGCCTGG
CACATGAACCGCTCCATCGTGCTGCTGCTCAAGGTGCTGGCCCAGCTGCGGGACCACAGC
ACCCTGCTGAAGGTGTCCTCCATGCTTCAGCGGACCCCAGACCAGGGCAAGAAGTATCTG
CGAGATGCTGACCGCCAGGTCCTGGCGCAGCGGGCCTTCATCCTCACTGTGAAGGTGCTC
GAAGACACGCTGAGCGAGCTCGCAGAGGGGTCAGAACGCCCAGGGCCCAAGGTCTGTGGC
CTCCCCGGAGCCAGGATGACCACCGATGTCTCACACAAGGCCAGTCCTGAGGATGGCCAG
GAGGGCCTCCCCCAGCCGAAGAAGCCCCCTCTGGCTGATGGCTCAGGGCCAGGGCCCGAG
CCAGGAGGCAAAGTGGGCCTCCTCAACCACCGGCCTGTGGCCATGGATGCAGGAGACAGT
GCAGACCAAAGCGGGGAGCGGAAGGATAAAGAGAGCCCACGGGCAGGGCCCACTGAGCCC
ATGGACACGAGTGAGGCCACTGTTTGCCACTCAGACTTGGAGCGGACACCACCCCTGCTG
CCAGGTCGCCCCGCAAGGGACCGGGGCCCCGAGAGCCGGCCCACTGAGCTGTCCCTGGAG
GAGCTGAGCATCAGTGCCCGGCAGCAGCCCACCCCGCTCACCCCAGCCCAGCCAGCCCCC
GCCCCCGCCCCCGCCACCACCACAGGGACCAGGGCAGGGGGCCACCCGGAGGAGCCGCTC
TCCCGGCTCAGCCGCAAGAGGAAGCTCCTGGAGGACACAGAGTCAGGCAAGACACTTCTG
TTGGATGCCTACCGTGTGTGGCAGCAGGGCCAGAAGGGTGTGGCCTATGACCTGGGCCGT
GTGGAGAGGATCATGTCGGAGACCTACATGCTCATCAAGCAGGTGGATGAGGAGGCTGCG
CTGGAGCAGGCTGTGAAGTTCTGCCAGGTCCATCTTGGGGCTGCCGCCCAGAGACAGGCC
TCGGGGGACACCCCCACCACTCCAAAGCACCCCAAAGACAGCCGAGAGAACTTCTTTCCT
GTGACAGTGGTGCCCACAGCCCCTGACCCTGTGCCAGCTGACTCTGTCCAGCGGCCCAGT
GATGCTCACACCAAGCCTCGCCCTGCACTAGCTGCCGCCACAACTATTATCACCTGCCCT
CCGTCAGCATCAGCTTCCACCCTGGACCAGTCCAAGGACCCTGGGCCTCCCCGGCCACAC
AGGCCTGAAGCTACCCCCAGCATGGCCTCTCTGGGCCCAGAGGGAGAAGAGCTGGCGAGA
GTGGCAGAGGGCACCAGCTTCCCGCCTCAGGAGCCACGGCACAGTCCGCAGGTGAAGATG
GCCCCCACAAGTTCCCCGGCAGAGCCACACTGCTGGCCGGCAGAGGCTGCCCTGGGCACA
GGCGCTGAGCCCACCTGCAGCCAGGAGGGGAAACTGAGGCCTGAGCCGAGAAGGGATGGG
GAGGCTCAGGAGGCTGCGAGTGAGACTCAGCCCCTGAGCTCTCCCCCAACAGCTGCCAGC
TCCAAGGCCCCCAGCAGTGGGAGTGCCCAGCCACCAGAGGGTCACCCAGGCAAGCCTGAG
CCCAGCCGGGCTAAGTCCCGCCCCCTGCCCAACATGCCAAAGCTGGTCATCCCCTCCGCC
GCCACCAAGTTCCCCCCTGAGATCACCGTCACGCCACCCACCCCAACCCTGCTCTCCCCC
AAAGGCAGCATCTCGGAGGAGACCAAGCAGAAGCTGAAGTCAGCCATCCTTTCTGCCCAG
TCTGCTGCCAACGTGAGGAAGGAGAGCCTATGCCAGCCAGCCCTGGAGGTCCTGGAGACA
TCCAGCCAGGAGTCCTCGCTGGAGAGCGAGACAGACGAGGACGACGACTACATGGACATT
TTG
Protein sequence predicted based on nucleotide sequence
MIRIAALNASSTIEDDHEGSFKSHKTQTKEAQEAEAFALYHKALDLQKHDRFEESAKAYH ELLEASLLREAVSSGDEKEGLKHPGLILKYSTYKNLAQLAAQREDLETAMEFYLEAVMLD STDVNLWYKIGHVALRLIRIPLARHAFEEGLRCNPDHWPCLDNLITVLYTLSDYTTCLYF ICKALEKDCRYSKGLVLKEKIFEEQPCLRKDSLRMFLKCDMSIHDVSVSAAETQAIVDEA LGLRKKRQALIVREKEPDLKLVQPIPFFTWKCLGESLLAMYNHLTTCEPPRPSLGKRIDL SDYQDPSQPLESSMVVTPVNVIQPSTVSTNPAVAVAEPVVSYTSVATTSFPLHSPGLLET GAPVGDISGGDKSKKGVKRKKISEESGETAKRRSARVRNTKCKKEEKVDFQELLMKFLPS RLRKLDPEEEDDSFNNYEVQSEAKLESFPSIGPQRLSFDSATFMESEKQDVHEFLLENLT NGGILELMMRYLKAMGHKFLVRWPPGLAEVVLSVYHSWRRHSTSLPNPLLRDCSNKHIKD MMLMSLSCMELQLDQWLLTKGRSSAVSPRNCPAGMVNGRFGPDFPGTHCLGDLLQLSFAS SQRDLFEDGWLEFVVRVYWLKARFLALQGDMEQALENYDICTEMLQSSTAIQVEAGAERR DIVIRLPNLHNDSVVSLEEIDKNLKSLERCQSLEEIQRLYEAGDYKAVVHLLRPTLCTSG FDRAKHLEFMTSIPERPAQLLLLQDSLLRLKDYRQCFECSDVALNEAVQQMVNSGEAAAK EEWVATVTQLLMGIEQALSADSSGSILKVSSSTTGLVRLTNNLIQVIDCSMAVQEEAKEP HVSSVLPWIILHRIIWQEEDTFHSLCHQQQLQNPAEEGMSETPMLPSSLMLLNTAHEYLG RRSWCCNSDGALLRFYVRVLQKELAASTSEDTHPYKEELETALEQCFYCLYSFPSKKSKA RYLEEHSAQQVDLIWEDALFMFEYFKPKTLPEFDSYKTSTVSADLANLLKRIATIVPRTE RPALSLDKVSAYIEGTSTEVPCLPEGADPSPPVVNELYYLLADYHFKNKEQSKAIKFYMH DICICPNRFDSWAGMALARASRIQDKLNSNELKSDGPIWKHATPVLNCFRRALEIDSSNL SLWIEYGTMSYALHSFASRQLKQWRGELPPELVQQMEGRRDSMLETAKHCFTSAARCEGD GDEEEWLIHYMLGKVAEKQQQPPTVYLLHYRQAGHYLHEEAARYPKKIHYHNPPELAMEA LEVYFRLHASILKLLGKPDSGVGAEVLVNFMKEAAEGPFARGEEKNTPKASEKEKACLVD EDSHSSAGTLPGPGASLPSSSGPGLTSPPYTATPIDHDYVKCKKPHQQATPDDRSQDSTA VALSDSSSTQDFFNEPTSLLEGSRKSYTEKRLPILSSQAGATGKDLQGATEERGKNEESL ESTEGFRAAEQGVQKPAAETPASACIPGKPSASTPTLWDGKKRGDLPGEPVAFPQGLPAG AEEQRQFLTEQCIASFRLCLSRFPQHYKSLYRLAFLYTYSKTHRNLQWARDVLLGSSIPW QQLQHMPAQGLFCERNKTNFFNGIWRIPVDEIDRPGSFAWHMNRSIVLLLKVLAQLRDHS TLLKVSSMLQRTPDQGKKYLRDADRQVLAQRAFILTVKVLEDTLSELAEGSERPGPKVCG LPGARMTTDVSHKASPEDGQEGLPQPKKPPLADGSGPGPEPGGKVGLLNHRPVAMDAGDS ADQSGERKDKESPRAGPTEPMDTSEATVCHSDLERTPPLLPGRPARDRGPESRPTELSLE ELSISARQQPTPLTPAQPAPAPAPATTTGTRAGGHPEEPLSRLSRKRKLLEDTESGKTLL LDAYRVWQQGQKGVAYDLGRVERIMSETYMLIKQVDEEAALEQAVKFCQVHLGAAAQRQA SGDTPTTPKHPKDSRENFFPVTVVPTAPDPVPADSVQRPSDAHTKPRPALAAATTIITCP PSASASTLDQSKDPGPPRPHRPEATPSMASLGPEGEELARVAEGTSFPPQEPRHSPQVKM APTSSPAEPHCWPAEAALGTGAEPTCSQEGKLRPEPRRDGEAQEAASETQPLSSPPTAAS SKAPSSGSAQPPEGHPGKPEPSRAKSRPLPNMPKLVIPSAATKFPPEITVTPPTPTLLSP KGSISEETKQKLKSAILSAQSAANVRKESLCQPALEVLETSSQESSLESETDEDDDYMDI L
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
