DNASU Plasmid | TM0460 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0460

Gene Name: peptide ABC transporter substrate-binding protein

Original Clone ID:

7258

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00089289

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1845nts

Open reading frame: 51 to 1895

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGAAGAAAC
TGTTTGTGCTGTTCCTGGCAGTCCTATCAGTTCTGGTACTGGCCGAAGTGAAGAACCCTG
ACACCATAATCGACGCCACCATTGGAGAACCCGACACTCTCGACCCACACTTCGCTTATG
ACACGGCGAGTGGTGAAGTTATCTACAACGTGTACGAGAACCTGATCGCTTACAAAGGAG
AGAGCCTCACAGAATTCGAACCACGCCTTGCGGAAAGATGGGAAATTCTGGACGACGGGA
AAACTTACAAGTTCTACATCAGGAAAGGCGTGAAGTTCCACGAAGGAGGAGATCTCACAC
CAGAAGACGTGGAATACAGCTTTGAAAGAGGTCTCATCTTCGATCCAACAGCGGGTCCCA
TGTGGATGCTCTGGGAAGCCCTGTTCGGTGTGGATTCACTGGAAACTTTCGTCGAGGAAA
AGATCGGCAAGCCTTATAGCGAACTCTTCGACGAAAACGGTGAGCCGCTTCCAGAGTACA
GAGACGCTCTCATAAAGATCTACACGGATTACATCGATCCCGCCATCGAAGTTGAAGGTG
ACGCCGTTGTGTTCCACCTTGTGAGACCCTTCGCACCGTTCATGTACATACTCGCCCAGA
GCGCCAGCTGGAGTGCTGTCCTCGACAAAGAGTGGTGTATAGAGATAGGATGCTGGGACG
GAAGAGCCGATACCTGGTGGAAGTACCACGATATCAGAAAAGAAGATTCTCCTCTCTACG
CGAGAATGAACGGAACTGGACCCTTCAAATTCGTCGAATGGGACAGAGCTCAGCAGAAAG
TCATCCTCGAGCGAAACGACAACTACTGGAGAGAACCCGCGAAGATCAAAAGAGTCATTA
TCTGGGGAATCGACGAGTGGAGCACAAGAAGGGCGATGTTCCTTCAGGGAGACGCCGATA
TCTGTGCTGTCCCAACCCAGTACCTCGAGCAGGTGGAAGGAAAACCCGGTGTCACCGTTG
TAAAGGGACTTCCTGAACTTGCGGTAACATCCCTTCACTTCGCGTGGAACGTTCCCGAAG
ACAGCAAGTACATAGGCTCTGGAAAACTCGACGGAAACGGAATACCACCCGATTTCTTCA
GCGATGAGAATGTGAGAAAAGCCTTCATCTACGCGTTCGACTACGACACATTCATAAACG
AAGTGCTCAAAGGTCTTGGTAGAAAGATACCAACAGACCTTCCAGAAGGACTCCTCGGAT
TCAACGAAGAGCTGCTGAACGATCCCGACGCTCCACACTTCGATATTGTGAAAGCAACGG
AGTACTTCAAGAAGGCATGGAACGGTGAAGTCTGGAAGAAAGGATTCAAGATCACGTTGC
TTTACAACACCGGAAATGAAGTGAGAAGACAAGCCGCAGAAATGCTGAAGGCATACATCG
AGATGATCAATCCGAAGTTTAAAGTTGAAGTGAGAGGCGTTCAGTGGCCTACGTATCTCG
ACGCAACCAAGAGAGGAGAAGTGCCTGCCTTCATCATAGGATGGCTCGCAGATTATCCGG
ATCCTCACAACTTCATCTTCACATACTACCACAGTGCAGGAGTTTACTCTGGAAGACAGG
GTGAAAACTTCAGAAAGTTTGTTTCCACACCACATCCCGACCTTGGTGGCAGAAGTCTCG
ACGAACTCATAGAAGAAGCGATCGCGAAGACCGATCCTGCAGAAAGACAGGCACTCTACG
AAGAGATCCAGAGGTTCGCAATGAAGCACGCCCTCGGTATGCCTCTCTATCAGCCGCTCG
GTGTGAGAGTCCAGAGAAGCTGGGTCAAAGGATGGTACCACAATCCAATGAGACCTGGCG
ACGACTACTACGTGCTCTGGAAAGCAGAAGAGTAAGTTTAAACGGTCTCCAGCTTGGCTG
TTTTGGCGGATGAGAGAAGATTTTC

Protein sequence predicted based on nucleotide sequence

MKKLFVLFLAVLSVLVLAEVKNPDTIIDATIGEPDTLDPHFAYDTASGEVIYNVYENLIA
YKGESLTEFEPRLAERWEILDDGKTYKFYIRKGVKFHEGGDLTPEDVEYSFERGLIFDPT
AGPMWMLWEALFGVDSLETFVEEKIGKPYSELFDENGEPLPEYRDALIKIYTDYIDPAIE
VEGDAVVFHLVRPFAPFMYILAQSASWSAVLDKEWCIEIGCWDGRADTWWKYHDIRKEDS
PLYARMNGTGPFKFVEWDRAQQKVILERNDNYWREPAKIKRVIIWGIDEWSTRRAMFLQG
DADICAVPTQYLEQVEGKPGVTVVKGLPELAVTSLHFAWNVPEDSKYIGSGKLDGNGIPP
DFFSDENVRKAFIYAFDYDTFINEVLKGLGRKIPTDLPEGLLGFNEELLNDPDAPHFDIV
KATEYFKKAWNGEVWKKGFKITLLYNTGNEVRRQAAEMLKAYIEMINPKFKVEVRGVQWP
TYLDATKRGEVPAFIIGWLADYPDPHNFIFTYYHSAGVYSGRQGENFRKFVSTPHPDLGG
RSLDELIEEAIAKTDPAERQALYEEIQRFAMKHALGMPLYQPLGVRVQRSWVKGWYHNPM
RPGDDYYVLWKAEE*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1895

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 282333

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0460

GENEID :

897491

Locus Tag :

TM0460

GI :

15643226

GenBank Accession :

NP_228270.1

Target GenBank:

NP_228270

Protein

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9WYT2

Clone

Cloning Information :

275751

HIP Master Clone ID :

205237

Original Clone ID :

7258

Experimental

TargetTrack - Experimental data :

282333

Organism

Species Specific ID:

897491