DNASU Plasmid | TM0324 (T. maritima ) in pMH1 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0324

Gene Name: hypothetical protein

Original Clone ID:

7172

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH1

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00089365

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1284nts

Open reading frame: 40 to 1323

CCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGATCATAGTTCTCATCGCC
TTTGCTGTTTTTCTGATGCTTGGAATGCCTGTTGCCTTTGCTATAGGAATATCCGGGTTT
CTCTGGTTTCTCCAACATCCTGAACTACCTATCACCATTCCGATACAGAGACTGCTTTCT
CAAACGGTGAATTTCACGTTGCTTGCTATTCCCATGTTCATTGTGGCTGGAAATGTGATG
AATTCTGCTGGAGTGACGAGAAGACTCCTCGATTTTGCCTCCACCCTCGTTGGTCACATG
AGAGGAGGACTCGGTCAGGTTTCAGCTGTTCTTTCCACGTTGATGGGAGGAGTTTCCGGA
TCCAGTATTGCAGACGCAGCTATGGAAACAAGGATGCTCGGTCCAGAGATGCTCAAACGA
GGTTATCCAAGAGGTTTCGCAGTTGCCGTGAATGTCTGGACGTCTCTGATCACCCCGATC
ATTCCCCCTGGTATCGCTTTCATCATTTACGGTACGATCGGTCAGGTCTCTATAGGGAGG
CTCTTCGCAGCAGGAATAGGACCCGGTCTTCTTTTGATGATCGTGTACATGATCACCATA
TGGTTTGTTGCCAAAAGATTGAATCTTGCACCCGAACGAGAGAGACGAGCACCGGTAAAT
GAAGTTTTCAGATCTCTGGGAAAGAGCATCTGGGCTCTTATGTTTCCGGTTTTGCTGATT
GTGGGTCTGAGAGGCGGTATCTTTACCCCTTCAGAGGTTGGTTCCTTCGCGGTTTTGTAC
GGAATGCTGGTGGGATTCATAATACACAGAGAGATGTCTCTTAAAACCTTTTACTGGGAA
ACCCTGGAGAATTCTCTTGGTGATATCGGGAGTGTGATGTTCATACTTTCGATGTCTACA
ATTTTCGGTTACGGTATGATCTGGGAAAGAATACCGGAAAAGCTCGCGGAATTTCTCCTG
GGAATATCTTCCAATCCAAACGTTCTGATGATAATGATCTCTTTATTCTTGGTTTTTGCC
GGTCTGTTTGTCGATGCAACGGCTTTGATTCTCATGCTCACGGCAATCTTGCTTCCTGTG
GCAGAACAGGTGGGAATAGATCCAGTTCACTTCGGTCTGGTATTCATTTTATCCGCTGCT
ATGGGTAACCAGACACCTCCTGTAGGTGCTTCTATGTACGCAGGTTGCTCGGTACTCGAT
GCGACTATGGAAGAGTACATACAGGCATCCTGGCCTTTCTTGTTTGTGACGATACTGGCG
ATACTTATAGTCATCTTCTTCCCACAGATCGTATTGTTCATTCCGAATCTCATCTTTGGA
TAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTC

Protein sequence predicted based on nucleotide sequence

MIIVLIAFAVFLMLGMPVAFAIGISGFLWFLQHPELPITIPIQRLLSQTVNFTLLAIPMF
IVAGNVMNSAGVTRRLLDFASTLVGHMRGGLGQVSAVLSTLMGGVSGSSIADAAMETRML
GPEMLKRGYPRGFAVAVNVWTSLITPIIPPGIAFIIYGTIGQVSIGRLFAAGIGPGLLLM
IVYMITIWFVAKRLNLAPERERRAPVNEVFRSLGKSIWALMFPVLLIVGLRGGIFTPSEV
GSFAVLYGMLVGFIIHREMSLKTFYWETLENSLGDIGSVMFILSMSTIFGYGMIWERIPE
KLAEFLLGISSNPNVLMIMISLFLVFAGLFVDATALILMLTAILLPVAEQVGIDPVHFGL
VFILSAAMGNQTPPVGASMYAGCSVLDATMEEYIQASWPFLFVTILAILIVIFFPQIVLF
IPNLIFG*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1323

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 282199

Recommended expression in:

Not Applicable

Vector Name:

pMH1

Synonyms:

pMH-1

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose inducible expression, adds N-terminal 6xHis tag; amp resistance; restriction enzyme (Pml I/Psi I) or PIPE cloning.

Comments:

None

Size (bp):

4048

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTTGGGCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTTATAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type bacterial origin of replication

1887

2569

inducible trxn activator protein gene

araC

araC cds

3144

4019

promoter

pBAD

pBAD promoter

250

277

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1130

1789

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

794

820

Gene

Gene Symbol :

TM0324

GENEID :

897269

Locus Tag :

TM0324

GI :

15643092

GenBank Accession :

NP_228135.1

Target GenBank:

NP_228135

Protein

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9WYF9

Clone

Cloning Information :

276418

HIP Master Clone ID :

205904

Original Clone ID :

7172

Experimental

TargetTrack - Experimental data :

282199

Organism

Species Specific ID:

897269