DNASU Plasmid | TM0587 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0587

Gene Name: manganese-dependent inorganic pyrophosphatase

Original Clone ID:

7337

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00089402

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1704nts

Open reading frame: 51 to 1754

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGGTCACGT
TCTCGATAAGGATTATATCATCTGATAGAATTTTTTTAGTAGCGGAGGTGAAAGCATTGG
AAAGAGTGTACGTGATAGGCCACAAGAATCCGGACACCGACAGCGTTTGCTCTGCGATAG
GGTACGCGCACTTCAAAAACAATGTGGAAAAGGGAAAAACATTCATTCCAGCTCGAAGCG
GCGATCTCACAAACGAATCCCTCTTCGTGCTCAAATATTTCGGAATGAATCCACCCCTCC
ACATTGAAACGCTCGAACCCACCGTTGAAGATCTCGAGCTGAAAAATCCCATTTTCGTCA
CTCCGGACACATCCGCCTACGATGTGGCCATGCTCATGGAAAGCAGAGGAATAAAAAACG
TTCCGGTGGTTTCAAAGGAGAAAATGATAGGAGTGGTCACTGAAAGCAACATTGCTCGAG
TCTACGTGAGGAGATTGAAGATAGAACCATTGGTTATACACCCGGTTCCGTTCGATCAGC
TAGTCAGGATACTGAAAGCAGAGGTTGTCTGTGACTACATGAAAGAAAAAACCGTATCCG
GAAAGGTTCACATAGCGGTGGATGCCCTTCATGTGCTCCTGGGAAAGATAGAGATAGGTG
ACGTGGTGATCGTGGGAGACAACGAACCGGCCCAGATCGCTCTTCTGGAAAAAGGAGCAA
AACTCATGATAGTCGTGAACAACGCCCCAGTATCAAACAGAGTGCTTGAAATAGCAAAAG
AAAAGAACGCCGCCGTTTTGAGAGTGAAGTTCGACGCGTTCAGCGCCGCAAAGCTCATAA
ACCTTTCACTCCCCGTGACCCTCGTGATGAGCAAGAAATTCCCCACGGTGACGAAGAAGG
ACACACTCGAGGAAGTAAAAGAGATCGTCTTCACCTCGAAGATAAGAGCAGCGTTCGTAG
AAGACGAGAAAGGGCGGCTTTGTGGTGTTATAACTAGGACGGACCTGCTCAAAGATGTGA
GGAAAAAGGTGATCCTTGTGGATCACAACGAGATCACCCAGGCACCGGAAGGAGTCGAGA
AAGCGGAAATCCTCGAGATCATAGACCATCACAGGCTCGGTGGACTGAGCACTCTGAATC
CCGTTTTCTTCTACAACGAACCCGTCGGAAGCACCTCAACAATAGTGGCAGAGTTCTTCT
TGAAAAACGGTGTGAAGATGGAAAGGGAGATAGCCGGAATTCTGCTCTCGGGTATCGTTT
CCGATACACTCTTCTTCAAACTCTCCACGACGACAGAGAAAGACAGGAAGATGGCGAATT
TCCTGGCTGATGTTGCCAAACTCGATCTGGAAAAATTCGCGAAGAAACTGCTGAAGGAAG
GGATGAAGATACCGGAAGACGTCGATCCCGCTGAACTGCTGAAGCGCGACGTGAAAGTCT
ACGAGATGGGAGAAGAATCCTTCGCCGTTTCTCAGATAATGACGTCGGACTTTTCAACAC
TTTTGAAAGAAAAGGAACGTTTCATGAACACACTGAAGACCCTCAAGGGAGAATTCGGTG
TCAAACATTTCTTTGTGCTCTTCACGAATCCTGTGGAAGAAGCGAGTCTTCTGATGATGG
ATGGAGATCAAAAGTTAGTGGAAAAAGCCTTCAACGCGGAAAAGAAGGACGGTCTTTTTC
TGTTGAAGGGGGTTATGTCCAGAAAGAAGGACTTCGTTCCCAAGATCGGTGAGGTGCTGA
GAAGGGAGAGATAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGAT
TTTC

Protein sequence predicted based on nucleotide sequence

MVTFSIRIISSDRIFLVAEVKALERVYVIGHKNPDTDSVCSAIGYAHFKNNVEKGKTFIP
ARSGDLTNESLFVLKYFGMNPPLHIETLEPTVEDLELKNPIFVTPDTSAYDVAMLMESRG
IKNVPVVSKEKMIGVVTESNIARVYVRRLKIEPLVIHPVPFDQLVRILKAEVVCDYMKEK
TVSGKVHIAVDALHVLLGKIEIGDVVIVGDNEPAQIALLEKGAKLMIVVNNAPVSNRVLE
IAKEKNAAVLRVKFDAFSAAKLINLSLPVTLVMSKKFPTVTKKDTLEEVKEIVFTSKIRA
AFVEDEKGRLCGVITRTDLLKDVRKKVILVDHNEITQAPEGVEKAEILEIIDHHRLGGLS
TLNPVFFYNEPVGSTSTIVAEFFLKNGVKMEREIAGILLSGIVSDTLFFKLSTTTEKDRK
MANFLADVAKLDLEKFAKKLLKEGMKIPEDVDPAELLKRDVKVYEMGEESFAVSQIMTSD
FSTLLKEKERFMNTLKTLKGEFGVKHFFVLFTNPVEEASLLMMDGDQKLVEKAFNAEKKD
GLFLLKGVMSRKKDFVPKIGEVLRRER*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1754

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 282460

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0587

GENEID :

897659

Locus Tag :

TM0587

GI :

15643353

GenBank Accession :

NP_228397.1

Target GenBank:

NP_228397

Protein

SMART domain :

CBS : Domain in cystathionine beta-synthase and other proteins.

SMART domain :

DHHA2 :

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9WZ56

Clone

Cloning Information :

276604

HIP Master Clone ID :

206090

Original Clone ID :

7337

Experimental

TargetTrack - Experimental data :

282460

Organism

Species Specific ID:

897659