Gene:
Gene Symbol:
TM1217
Gene Name:
oxidoreductase
Original Clone ID:
7714
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00089478
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1857nts
Open reading frame: 51 to 1907
AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGAGAAAAA
TGGCAGTCCAGAAGCTACCAGAAAAGGATTTCTTTGCTCCTCTGAAGGCATGGAAATTTC
TTGTCAGAAAACCGGTTACAATAGAGGTTCCAAACAAAATCAGGAGAGAAGCTTCGGAAA
GATACAGAGGATTCCACGTGAACGACTGGGGAAAGTGCATAGGGTGCGGTACATGCGCAA
AGATCTGCCCAACCGATGCCATAACCATGGTGGAGGTCCCGGATCTCACTCAAGAAGATG
GAAAACTTCCGCAAAGACCCGTCATAGATTACGGCAGGTGTTCTTTCTGTGCGCTCTGCG
TTGACATCTGCACAACGGGTTCACTCAAGATGACGAGGGAATACATCCACATCTCGGAAG
ATCCGGAAGACTTCATCTTTATGCCAACGGATAAGGGATTGAACGCGAAAAAAGGTCTCT
ACGAATTTGGAAAGGCACCCCTCGGCTGGGTGAGGGATGAGAACTCTGAGCTGCTCGATC
TCGAAAGAATAGAAATGCCCGTGGAACCACCGGAAGTCAGGATCAAATCTTTCATAGAAA
TCGTGAAAGGATACAGTAGAGAACAGGCCATGCAGGAGGCCGCCAGGTGTGTGGAGTGCG
GTGTGTGTACCTACACCTGTCCCGAACACATGGACATACCCCAGTACATAAAATCCGTGT
ACGAGGACAATCTGGAAGAAGGACTGAGATGGCTCTACAGAACGAATCCTCTCTCAATGG
TCTGTGGAAGGGTCTGTACTCACAGGTGTGAAACCGCCTGTTCTGTGGGAGTCAGAGGAG
AACCCGTTGCGATTCAGTGGTTGAAAAGGTACATAGTGGACAGCATTCCTCTCGAGAGAT
ACGGAGAGATCCTGGACATAAAACCGGAGAGAAAAGGAAAGAGTGTGGGAATCATAGGAT
CGGGTCCCGCTGGGCTCGCCGCAGCCTACTTTCTTGCCACCATGGGATACGATGTTACCA
TATACGAATCGGAATCAAAACCTGGTGGTGTGATGAGGTACGGTATTCCAAAATACCGAC
TTCCCGATGAAGCGCTGGACAAGGATATCGCATTCATCGAGGCGCTCGGGGTGAAAATTC
TACTCAACACGAGAGTCGTCCAGGATGTGTCTTTCGAAGATGTCAGAAAGAGACACGATG
TTGTCTTTCTTGCAACAGGCTTCGGTCTTGGAAGGTCAACGGGTGTTCCGGGGACCGATC
ACCCAGATGTGAAACAGGCACTCCCCTTGCTCAAGATGATAAGGGACTACCTGAGGGGAG
ATGGTCCAAAGCCACCGGTCCCAGAGAGACTCGTTGTCATCGGTGGAGGAAACGTCGCGA
TGGATATCTCCAGGTCGATGGCAAGACTTCAGTTCATGGAGTACGGAAGGGTGAACGTTC
AGGTGCTTTCACTTGAAGGATGCTTCGAGGACATGCCGGCTGATATGGAAGAAATAGAAG
AGGCTCTCGAAGAGGGTATCGTCATAAATCCAGGATGGGGACCGATGGAAGTCGTGATAG
AGAACGACAGGATAAAAGGTGTGAAATTCAAAAAATGTGTCAGTGTGTTCGACGGCGAAG
GACGGTTCAATCCACAGTTTGACGAAAAAGAACAAATCTTTGTAGAAGCGGACATGGTCG
TAGAAGCGATTGGACAGGCACCAGATTATTCCTACTTACCCGAAGAAATACGAAGCAAAC
TCGAATTTTTCAGAGGAAGAATCAAAACAAACGAGTACAATCAGACCTCTGTGGAATGGT
TGTTTGCAGGAGGAGACATCGTTCACGGACCGGATATCATTCACGGTATTGCAGATGGAT
ACGCCGCGGCAAAAGGGATAGACATGTACCTGAGAAAGGAAAAATAAGTTTAAACGGTCT
CCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTC
Protein sequence predicted based on nucleotide sequence
VRKMAVQKLPEKDFFAPLKAWKFLVRKPVTIEVPNKIRREASERYRGFHVNDWGKCIGCG TCAKICPTDAITMVEVPDLTQEDGKLPQRPVIDYGRCSFCALCVDICTTGSLKMTREYIH ISEDPEDFIFMPTDKGLNAKKGLYEFGKAPLGWVRDENSELLDLERIEMPVEPPEVRIKS FIEIVKGYSREQAMQEAARCVECGVCTYTCPEHMDIPQYIKSVYEDNLEEGLRWLYRTNP LSMVCGRVCTHRCETACSVGVRGEPVAIQWLKRYIVDSIPLERYGEILDIKPERKGKSVG IIGSGPAGLAAAYFLATMGYDVTIYESESKPGGVMRYGIPKYRLPDEALDKDIAFIEALG VKILLNTRVVQDVSFEDVRKRHDVVFLATGFGLGRSTGVPGTDHPDVKQALPLLKMIRDY LRGDGPKPPVPERLVVIGGGNVAMDISRSMARLQFMEYGRVNVQVLSLEGCFEDMPADME EIEEALEEGIVINPGWGPMEVVIENDRIKGVKFKKCVSVFDGEGRFNPQFDEKEQIFVEA DMVVEAIGQAPDYSYLPEEIRSKLEFFRGRIKTNEYNQTSVEWLFAGGDIVHGPDIIHGI ADGYAAAKGIDMYLRKEK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1907
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DL41
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 283082
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
