DNASU Plasmid | TM0068 (T. maritima ) in pMH2T7 (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TM0068

Gene Name: D-mannonate oxidoreductase

Original Clone ID:

17227

Keyword:

None

Species:

Thermotoga maritima

Type:

cDNA

Vector Name:

pMH2T7

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

TmCD00089856

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1617nts

Open reading frame: 51 to 1667

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGCGTCTCA
ACAGGGAAACGATAAAAGATAGAGCGGCTTGGGAAAAGATAGGTGTCAGGCCTCCGTATT
TCGATCTCGACGAAGTAGAGAAAAATACAAAAGAACAACCGAAATGGGTTCATTTCGGTG
GTGGAAACATATTCAGGGGATTCGTTGCGGCAGTGCTTCAGAATCTCCTCGAAGAGGGAA
AGGAAGACACTGGTATAAATGTGATAGAACTCTTCGACTACGAAGTCATAGACAAAGTTT
ACAAACCGTACGACAATTTATCGATAGCGGTCACAATAAAACCCGACGGAGATTTTGAAA
AGAGAATCATAGCGAGCGTGATGGAAGCTCTCAAGGGAGATCCTTCGCATCCAGACTGGG
AAAGAGCAAAAGAAATCTTCAGAAATCCTTCTCTTCAGCTCGCTTCTCTCACCATCACAG
AAAAAGGCTACAACATAGAAGATCAGGCCGGGAACCTCTTTCCTCAGGTGATGGAGGACA
TGAAAAACGGTCCTGTCTCACCCCAAACATCGATGGGAAAAGTGGCTGCACTTCTTTACG
AAAGATTCAAAGCGGGAAGACTTCCTATCGCTCTTCTCAGCCTCGATAATTTCTCAAGAA
ATGGCGAAAAACTCTACAGCTCTGTGAAAAGAATTTCCGAGGAGTGGGTTAAAAGCGGTC
TGGTGGAAAAAGATTTCATTGATTATCTGGAAAAAGACGTTGCTTTCCCATGGAGTATGA
TAGATAAGATTGTACCGGGTCCTTCAGAATTCATAAAAGAGCACTTAGAAAAACTTGGAA
TAGAGGGAATGGAAATCTTTGTGACATCCAAGAGGACCCACATCGCTCCTTTCGTAAACA
TGGAATGGGCTCAGTATCTGGTGATAGAAGACAGCTTTCCGAACGGCCGGCCAAAACTCG
AAGGAGCAGATAGGAACGTTTTTCTGACAGACAGAGAAACTGTTGAGAAGGCAGAAAGAA
TGAAGGTGACAACCTGTCTCAATCCTCTTCACACAGCGCTGGCCATCTTCGGTTGCCTTC
TGGGATACAAAAAGATCGCAGACGAAATGAAAGACCCCCTCCTGAAAAAACTTGTAGAAG
GTGTGGGAGAAGAGGGAATAAAAGTTGTGGTAGATCCTGGTATCATAAATCCAAGAGAAT
TCTTGAACGAAGTGATAAACATCAGACTTCCCAATCCGTATCTGCCAGACACACCGCAGA
GAATAGCAACGGATACCTCTCAAAAGATGCCTATCAGGTTCGGCGAAACTATAAAGGCAT
ACCACGAAAGACCAGATCTGGATCCAAGGAATCTGAAATACATACCACTTGTGATAGCGG
GATGGTGCAGATACCTCATGGGAATAGACGATGAAGGAAGAGAAATGCAGCTCAGTCCAG
ATCCCCTTCTTGAGAACCTGAGGTCTTACGTTTCAAAGATAAAATTCGGCGATCCCGAAT
CCACGGATGATCATCTGAAACCGATCCTTTCCAGCCAGCAGCTTTTCAGAGTGAACCTGT
ACGAAGTAGGACTTGGAGAAAAAATAGAAGAACTGTTCAAGAAGATGATCACAGGGCCAC
GAGCTGTGAGGAAAACACTCGAAGAAGTCGTTGGGAGGGAAGATGGATAATTAATTAACT
TTAAGAAGGAGATATACATACCCGGGTAAGTTTAAAC

Protein sequence predicted based on nucleotide sequence

VRLNRETIKDRAAWEKIGVRPPYFDLDEVEKNTKEQPKWVHFGGGNIFRGFVAAVLQNLL
EEGKEDTGINVIELFDYEVIDKVYKPYDNLSIAVTIKPDGDFEKRIIASVMEALKGDPSH
PDWERAKEIFRNPSLQLASLTITEKGYNIEDQAGNLFPQVMEDMKNGPVSPQTSMGKVAA
LLYERFKAGRLPIALLSLDNFSRNGEKLYSSVKRISEEWVKSGLVEKDFIDYLEKDVAFP
WSMIDKIVPGPSEFIKEHLEKLGIEGMEIFVTSKRTHIAPFVNMEWAQYLVIEDSFPNGR
PKLEGADRNVFLTDRETVEKAERMKVTTCLNPLHTALAIFGCLLGYKKIADEMKDPLLKK
LVEGVGEEGIKVVVDPGIINPREFLNEVINIRLPNPYLPDTPQRIATDTSQKMPIRFGET
IKAYHERPDLDPRNLKYIPLVIAGWCRYLMGIDDEGREMQLSPDPLLENLRSYVSKIKFG
DPESTDDHLKPILSSQQLFRVNLYEVGLGEKIEELFKKMITGPRAVRKTLEEVVGREDG

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1667

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DL41

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in 281949

Recommended expression in:

Not Applicable

Vector Name:

pMH2T7

Synonyms:

pMH-2-T7

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.

Comments:

None

Size (bp):

4084

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGTGTAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColEI origin of replication

1921

2603

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

selectable marker

AmpR

ampicillin resistance gene

1164

1823

tag

His

N-terminal 6x His tag

367

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

Gene

Gene Symbol :

TM0068

GENEID :

896892

Locus Tag :

TM0068

GI :

15642843

GenBank Accession :

NP_227884.1

Target GenBank:

NP_227884

Protein

Structural Annotation Wiki :

TOPSAN

Swissprot ID :

Q9WXS3

Clone

Cloning Information :

281030

HIP Master Clone ID :

210516

Original Clone ID :

17227

Experimental

TargetTrack - Experimental data :

281949

Organism

Species Specific ID:

896892