Gene:
Gene Symbol:
TM0214
Gene Name:
glycine dehydrogenase subunit 2
Original Clone ID:
17235
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH1
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00089903
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1422nts
Open reading frame: 51 to 1472
AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGACGATAT
TTGAGAGATCTAAAAAGGGAAGAAAAGCATTCCGGCTTCCCGAGAGTGACATTCCAGAAT
ATTCCCTTCCGGATCGATTTCTCAGGAGGACACCTCCGGAGCTTCCCGAGGTGAGCGAGC
CTGATTTGGTGAGGCATTACACCAATCTTGCCAGAAAGAACTACTCTGTGGATCTCGGAA
TCTACCCCCTCGGTTCCTGCACCATGAAGTACAATCCCAAACTGAACGAAAAAGCTGCGA
ATCTCGAAGGATTCAGAGAGATACACCCGTATCAGCCCGTCGAAACTGTTCAGGGCAGTC
TTCGGCTCATGTACGAACTGAAAAAAATGTTGTGCGAGATAACGGGAATGGATGATATGA
CACTTCAACCCGCTGCCGGAGCTCACGGAGAACTCACGGGAATGCTGATAGTGAGAGAGT
ACTTTAAAAACAGAGGGGACACTGGAAGAAAGAAAGTTCTCGTACCGGATTCTGCCCACG
GTACCAACCCAGCATCTGCTTCGATGGTTGGTTTTGAGGTGGTGGAGATAAAGAGCAAAA
ACGGTATGGTGGATGTGGAAGATTTGAAAAAACTACTGGATGAAGAAGTAGCCGCTGTGA
TGCTCACAAATCCAAACACGCTTGGGCTCTTCGAAAAGGACATACTGAAAATAGCTGAAA
TGACGCATGAGTGTGGTGCCCTTCTCTATTACGATGGTGCGAACCTCAACGCGATCATGG
GTAAAGTGAGACCAGGAGACATGGGGTTCGACATCGTCCATTTGAACCTGCACAAGACGT
TTTCCACTCCCCACGGAATGGGAGGGCCCGGATCTGTGCCAGTTGGTGTGAAAAAGCATC
TGGTGGATTTTCTGCCCTTCCCCCAGGTAAAGAAGAACGGAGAACTTTACGAACTCTTCG
TTCCTGAAAAGACCATAGGACGTGTGAGGAGTTTCTTTGGAAATTTCCCGGTTCTCGTAA
AAGCGTACACGTACATTCTCACGATGGGAAGAGACGGCCTTGAAAGAGTGAGTGAAATGG
CCGTTTTGAACGCGAACTACTTGAAGAAAAAAATAGAGAAGTTCCTGGAGATTCCATACA
ATGGGTTCTGCATGCATGAATTCGTGGCGAGCGCGGAGAAAGTGTTCAGGGAAACCGGTG
TGAGAACACTGGATATTGCGAAGCGAATACTGGACTTTGGTGTGCATCCTCCCACAGTTT
ACTTTCCACTCATCGTTCCGGAAGCACTCATGATAGAACCAACTGAGACTGAAAACAAAG
AAACGCTGGATAAGTACGCCGAAATCCTTGAAAGAGTGGTAAAAGAAGCCTATGAGAATC
CTGATGCTTTGAAAAACGCTCCTCACAACACACCGGTTCGAAGGGTCAACGAAGTGCTCG
CTTCGAAGAAACCCGTGTTCAGGTGGAGGGGATAAGTTTAAACGGTCTCCAGCTTGGCTG
TTTTGGCGGATGAGAGAAGATT
Protein sequence predicted based on nucleotide sequence
MTIFERSKKGRKAFRLPESDIPEYSLPDRFLRRTPPELPEVSEPDLVRHYTNLARKNYSV DLGIYPLGSCTMKYNPKLNEKAANLEGFREIHPYQPVETVQGSLRLMYELKKMLCEITGM DDMTLQPAAGAHGELTGMLIVREYFKNRGDTGRKKVLVPDSAHGTNPASASMVGFEVVEI KSKNGMVDVEDLKKLLDEEVAAVMLTNPNTLGLFEKDILKIAEMTHECGALLYYDGANLN AIMGKVRPGDMGFDIVHLNLHKTFSTPHGMGGPGSVPVGVKKHLVDFLPFPQVKKNGELY ELFVPEKTIGRVRSFFGNFPVLVKAYTYILTMGRDGLERVSEMAVLNANYLKKKIEKFLE IPYNGFCMHEFVASAEKVFRETGVRTLDIAKRILDFGVHPPTVYFPLIVPEALMIEPTET ENKETLDKYAEILERVVKEAYENPDALKNAPHNTPVRRVNEVLASKKPVFRWRG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1472
Mutations:
None
Discrepancy:
g666t,A222A;g827t,G276V;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DL41
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 282094
Recommended expression in:
Not Applicable
Vector Name:
pMH1
Synonyms:
pMH-1
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose inducible expression, adds N-terminal 6xHis tag; amp resistance; restriction enzyme (Pml I/Psi I) or PIPE cloning.
Comments:
None
Size (bp):
4048
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1887
2569
inducible trxn activator protein gene
araC
araC cds
3144
4019
promoter
pBAD
pBAD promoter
250
277
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1130
1789
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcription terminator
794
820
