Gene:
Gene Symbol:
TM1134
Gene Name:
hypothetical protein
Original Clone ID:
24483
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7TEV
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00089922
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1257nts
Open reading frame: 51 to 1307
CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGTTCCATG
ATGTGTTGTTTGGTAGCGCTTTACTTCTGGTTCTGATGAAAGATATCAACCTCTACTCTC
TGTTGCTCCTTTCAGGCTCAGCGGTAACGGCGATACTAAAGAGAATCTCCAGAGCCTACC
TGATCGTTCTTCCGGTCTCAGGGATCCTCCTCTGGATACTTTCGAAAGAAAAAGACCTGG
TTTTCGCATCTCTCATAGTTACTTTCTTCCTTGTGTTTTCAGATTTCGAAAATGAAAGGT
CAAAAGTGTATTCGCTGGCCTTCTTTTTCGTGCTTTCGTTGATATTTGAGAAAAATCCAT
CTTCATTCCTTTTTGTCTCCGGAGCTATGGCCCTCTTTTTCCTTCATGAGAAGAAGTTCC
TCTACGCTTCTGTGATTCCACTTCTGGCTTTTGTTCCAGAATTCAGACTTCCATTCCTTC
TTCCAGAAACGCATCAGCCAGAAAGTGGTGAATCGGTCCAACAGATGGTGAAAAACGTTG
TGGTCGTGATAGAAAAATTCACCCAGAGTGGTAGTCTTGCTATGAAACTTCTCTGGGAAG
TCATTTTCTGGGTCGCTGTGGCAGGTGTGGTGCTACTCGTTGTTGTTTTCTTCAAAGAGT
TTCGAATGAATTTAAAGGTGTCACATTACGTCTTTGCCGCTCTCGGTGTTTTTGTGGTTA
GCTTTTATCTGTTCGTGTTGTTCGTGAACATGGTGGTTCAGAACGTGAACCTCGACGTAG
GAATTCCAGAGCTCACACCAGTAGGTCAGATGCTTTCGTCCACCTCTTCCGCATCTGTGA
CCGTCGTTGAAATCGAAAGAAATCCTTCGTGGAATTCGGTAAGATGGGTTTTAACCGCTG
TTTCTCTTTTCGTGGTGGTATTGTTCTTCCACACCACTTTGAAGCTCTTCCTTAGAACGT
TGAAAACGTCGAGCGAAGAAATTGAAACGGAAGAATCGAGAGAGGAAGAAAAAGAAGATA
TTGAAGAAAAGGAACATCACACTGAACGAAGAGTAATACACACGTTAGAAGACGCGTACC
TCTTCCTGAGGTGGAAATATTTTCCAGGAAAGGAACATCTCACTCCGTACGAGTTGATAT
CCGGGAAAAAGTTTCGACACTTTAGAGATTTGACCGAGCTGTACGTCGAAAGTAAATATG
CAGGAAAAGGAATAAAACTGCCTGAGAAAGAAATAGCAAAGATTTTCTCGGAATCAGAGA
AAGAACTGAAAAAGACAAACATTAACCTCCAGAAAAATCTGCATTAAGTTTAAACGGTCT
CCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTC
Protein sequence predicted based on nucleotide sequence
MFHDVLFGSALLLVLMKDINLYSLLLLSGSAVTAILKRISRAYLIVLPVSGILLWILSKE KDLVFASLIVTFFLVFSDFENERSKVYSLAFFFVLSLIFEKNPSSFLFVSGAMALFFLHE KKFLYASVIPLLAFVPEFRLPFLLPETHQPESGESVQQMVKNVVVVIEKFTQSGSLAMKL LWEVIFWVAVAGVVLLVVVFFKEFRMNLKVSHYVFAALGVFVVSFYLFVLFVNMVVQNVN LDVGIPELTPVGQMLSSTSSASVTVVEIERNPSWNSVRWVLTAVSLFVVVLFFHTTLKLF LRTLKTSSEEIETEESREEEKEDIEEKEHHTERRVIHTLEDAYLFLRWKYFPGKEHLTPY ELISGKKFRHFRDLTELYVESKYAGKGIKLPEKEIAKIFSESEKELKKTNINLQKNLH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1307
Mutations:
None
Discrepancy:
ins@1,21;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 283000
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7TEV
Synonyms:
pMH-2-T7-TEV
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; amp resistance; PIPE cloning.
Comments:
None
Size (bp):
4105
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1942
2624
inducible trxn activator protein gene
araC
araC gene
3199
4074
promoter
T7
T7 promoter
290
310
protease cleavage site
TEV
TEV protease cleavage site
382
402
selectable marker
AmpR
ampicillin resistance gene
1185
1844
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcription termination
849
875
