Gene:
Gene Symbol:
TM0554
Gene Name:
3-isopropylmalate dehydratase large subunit
Original Clone ID:
14578
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH4
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00089994
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1254nts
Open reading frame: 51 to 1304
AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGACACTCG
CAGAAAAGATACTCTCTCAAAAAGCGGGAAGAAAGGTGGAACCGGGAGAATTTCTCCTTC
TGGAGCCGGACATCGCTCTCGCGAACGACATAACGGCTCCGCTTGCGATAAAGAAGTTCA
AGGAGTACGGTGGAAAGAAAGTGAAGTATCCAGACAGAGTGGTGCTCGTTCCAGACCATT
TCACCCCCAACAAGGACATAAAGTCCGCCATGCAGGTGAAGATGATGAGAGAATTTGCCA
GAGAGCAGGGGATCGAAAAGTTCTTCGAAATAGGCAGGATGGGTATTGAACACGTGCTGC
TTCCAGAAGAAGGAATCGTGAAATCCGGAGATCTCGTGGTCGGTGCGGATTCACACACCT
GTACCTACGGAGCCCTTGGAGCGTTTGCGACCGGAGTGGGGTCCACCGACATCGCGGGCT
TCTATCTCATAGGAAAGGTATGGTTCCGTGTCCCCGAGAGTATAAAGGTGACTCTTCGCG
GGAAGTTCAAGGATCTGGTGACTGCCAAAGACCTCGTGTTGAAGCTCATCTCGATACTCG
GTGTAGATGGAGCCAACTACAAGGCGATAGAATTCTCTGGACCGGGTGTGAAAGAGATCA
GCATGGACGGCCGTTTTACCATTTCGAACATGGCGATCGAGGCGGGAGGAAAGACGGGAC
TGTTTCCGGTGGACGAGATCACCATTGCCTACGAAAGAGAAAGAGGCATAGAAGTGGAAG
AGATGTACCCGGACGAAGACGCAAAATACGTGAGAGAAGTGGAAATGGACCTTTCTGAGC
TCGAACCTCAGGTTGCATATCCCTTCCTCCCATCCAACGCGAAGGACGTTTCCGAAGCTG
AGAAAGAGAGAATAAAGATAGATCAGGCGGTGATAGGAAGCTGTACCAACGGAAGGATAG
AAGATCTCAGACTTGCCGCGCAGATTCTGAAGGGAAGGACAGTATCACCGGACGTTCGAT
GCATCATAATTCCGGGATCCCAGAAGGTTTACAAACAGGCTCTCAAAGAAGGACTCATCG
ACATATTCATCGACGCGGGCTGCGCCGTTTCCACGCCCACATGTGGACCCTGTCTTGGCG
GACACATGGGAGTGCTCGCAGAAGGAGAGGTTGCGATCTCCACAACGAACAGGAACTTCG
TTGGAAGGATGGGACATCCGAACAGCAAGGTTTTCCTGGCATCTCCTGCAGTGGCGGCGG
CCAGCGCAATAAAAGGATACATCGCAGATCCGAGAAAGCTGTAACGCGACTTAATTAACT
CTAGATAACTGAGAAGGAGATATACATACCCGGG
Protein sequence predicted based on nucleotide sequence
MTLAEKILSQKAGRKVEPGEFLLLEPDIALANDITAPLAIKKFKEYGGKKVKYPDRVVLV PDHFTPNKDIKSAMQVKMMREFAREQGIEKFFEIGRMGIEHVLLPEEGIVKSGDLVVGAD SHTCTYGALGAFATGVGSTDIAGFYLIGKVWFRVPESIKVTLRGKFKDLVTAKDLVLKLI SILGVDGANYKAIEFSGPGVKEISMDGRFTISNMAIEAGGKTGLFPVDEITIAYERERGI EVEEMYPDEDAKYVREVEMDLSELEPQVAYPFLPSNAKDVSEAEKERIKIDQAVIGSCTN GRIEDLRLAAQILKGRTVSPDVRCIIIPGSQKVYKQALKEGLIDIFIDAGCAVSTPTCGP CLGGHMGVLAEGEVAISTTNRNFVGRMGHPNSKVFLASPAVAAASAIKGYIADPRKL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1304
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 282427
Recommended expression in:
Not Applicable
Vector Name:
pMH4
Synonyms:
pMH-4
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
5489
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1 bacterial origin of replication
3331
4013
inducible trxn activator protein gene
araC
araC cds
5463
4585
negative selection marker
ccdB
ccdB negative selection gene (may be gone in with-insert form)
1576
1785
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
153
124
selectable marker
CmR
chloramphenicol resistance gene (CmR)
484
1140
selectable marker
AmpR
ampicillin resistance gene
2574
3233
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcription terminator
2238
2264
