SLC3A1 (Homo sapiens) in pLenti6.3/V5-DEST

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLenti6.3/V5-DEST
Format: 
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00940569
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2058nts
Open reading frame: 77 to 2134

GACCAAGCATTCAGCAAGCCACTCTTCCACCTCCCTTACTGCAGGAAGGCACTCCGAAGA
CATAAGTCGGTGAGACATGGCTGAAGATAAAAGCAAGAGAGACTCCATCGAGATGAGTAT
GAAGGGATGCCAGACAAACAACGGGTTTGTCCATAATGAAGACATTCTGGAGCAGACCCC
GGATCCAGGCAGCTCAACAGACAACCTGAAGCACAGCACCAGGGGCATCCTTGGCTCCCA
GGAGCCCGACTTCAAGGGCGTCCAGCCCTATGCGGGGATGCCCAAGGAGGTGCTGTTCCA
GTTCTCTGGCCAGGCCCGCTACCGCATACCTCGGGAGATCCTCTTCTGGCTCACAGTGGC
TTCTGTGCTGGTGCTCATCGCGGCCACCATAGCCATCATTGCCCTCTCTCCAAAGTGCCT
AGACTGGTGGCAGGAGGGGCCCATGTACCAGATCTACCCAAGGTCTTTCAAGGACAGTAA
CAAGGATGGGAACGGAGATCTGAAAGGTATTCAAGATAAACTGGACTACATCACAGCTTT
AAATATAAAAACTGTTTGGATTACTTCATTTTATAAATCGTCCCTTAAAGATTTCAGATA
TGGTGTTGAAGATTTCCGGGAAGTTGATCCCATTTTTGGAACGATGGAAGATTTTGAGAA
TCTGGTTGCAGCCATACATGATAAAGGTTTAAAATTAATCATCGATTTCATACCAAACCA
CACGAGTGATAAACATATTTGGTTTCAATTGAGTCGGACACGGACAGGAAAATATACTGA
TTATTATATCTGGCATGACTGTACCCATGAAAATGGCAAAACCATTCCACCCAACAACTG
GTTAAGTGTGTATGGAAACTCCAGTTGGCACTTTGACGAAGTGCGAAACCAATGTTATTT
TCATCAGTTTATGAAAGAGCAACCTGATTTAAATTTCCGCAATCCTGATGTTCAAGAAGA
AATAAAAGAAATTTTACGGTTCTGGCTCACAAAGGGTGTTGATGGTTTTAGTTTGGATGC
TGTTAAATTCCTCCTAGAAGCAAAGCACCTGAGAGATGAGATCCAAGTAAATAAGACCCA
AATCCCGGACACGGTCACACAATACTCGGAGCTGTACCATGACTTCACCACCACGCAGGT
GGGAATGCACGACATTGTCCGCAGCTTCCGGCAGACCATGGACCAATACAGCACGGAGCC
CGGCAGATACAGGTTCATGGGGACTGAAGCCTATGCAGAGAGTATTGACAGGACCGTGAT
GTACTATGGATTGCCATTTATCCAAGAAGCTGATTTTCCCTTCAACAATTACCTCAGCAT
GCTAGACACTGTTTCTGGGAACAGCGTGTATGAGGTTATCACATCCTGGATGGAAAACAT
GCCAGAAGGAAAATGGCCTAACTGGATGATTGGTGGACCAGACAGTTCACGGCTGACTTC
GCGTTTGGGGAATCAGTATGTCAACGTGATGAACATGCTTCTTTTCACACTCCCTGGAAC
TCCTATAACTTACTATGGAGAAGAAATTGGAATGGGAAATATTGTAGCCGCAAATCTCAA
TGAAAGCTATGATATTAATACCCTTCGCTCAAAGTCACCAATGCAGTGGGACAATAGTTC
AAATGCTGGTTTTTCTGAAGCTAGTAACACCTGGTTACCTACCAATTCAGATTACCACAC
TGTGAATGTTGATGTCCAAAAGACTCAGCCCAGATCGGCTTTGAAGTTATATCAAGATTT
AAGTCTACTTCATGCCAATGAGCTACTCCTCAACAGGGGCTGGTTTTGCCATTTGAGGAA
TGACAGCCACTATGTTGTGTACACAAGAGAGCTGGATGGCATCGACAGAATCTTTATCGT
GGTTCTGAATTTTGGAGAATCAACACTGTTAAATCTACATAATATGATTTCGGGCCTTCC
CGCTAAAATAAGAATAAGGTTAAGTACCAATTCTGCCGACAAAGGCAGTAAAGTTGATAC
AAGTGGCATTTTTCTGGACAAGGGAGAGGGACTCATCTTTGAACACAACACGAAGAATCT
CCTTCATCGCCAAACAGCTTTCAGAGATAGATGCTTTGTTTCCAATCGAGCATGCTATTC
CAGTGTACTGAACATACTGTATACCTCGTGTTAGGCACCTTTATGAAGAGATGAAGACAC
TGGCATTTCAGTGGGATTGTAAGCATTTGTAATAGCTTCATGTACAGCATGCTGCTTGGT
GAACAATCATTAATTCTTCGATATTTCTGTAGCTTGAATGTAACCGCTTTAAGAAAGGTT
CTCAAATGTTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MAEDKSKRDSIEMSMKGCQTNNGFVHNEDILEQTPDPGSSTDNLKHSTRGILGSQEPDFK
GVQPYAGMPKEVLFQFSGQARYRIPREILFWLTVASVLVLIAATIAIIALSPKCLDWWQE
GPMYQIYPRSFKDSNKDGNGDLKGIQDKLDYITALNIKTVWITSFYKSSLKDFRYGVEDF
REVDPIFGTMEDFENLVAAIHDKGLKLIIDFIPNHTSDKHIWFQLSRTRTGKYTDYYIWH
DCTHENGKTIPPNNWLSVYGNSSWHFDEVRNQCYFHQFMKEQPDLNFRNPDVQEEIKEIL
RFWLTKGVDGFSLDAVKFLLEAKHLRDEIQVNKTQIPDTVTQYSELYHDFTTTQVGMHDI
VRSFRQTMDQYSTEPGRYRFMGTEAYAESIDRTVMYYGLPFIQEADFPFNNYLSMLDTVS
GNSVYEVITSWMENMPEGKWPNWMIGGPDSSRLTSRLGNQYVNVMNMLLFTLPGTPITYY
GEEIGMGNIVAANLNESYDINTLRSKSPMQWDNSSNAGFSEASNTWLPTNSDYHTVNVDV
QKTQPRSALKLYQDLSLLHANELLLNRGWFCHLRNDSHYVVYTRELDGIDRIFIVVLNFG
ESTLLNLHNMISGLPAKIRIRLSTNSADKGSKVDTSGIFLDKGEGLIFEHNTKNLLHRQT
AFRDRCFVSNRACYSSVLNILYTSC*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable